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The map label for this gene is 59711666

Identifier: 59711666

GI number: 59711666

Start: 1168949

End: 1170616

Strand: Direct

Name: 59711666

Synonym: VF_1059

Alternate gene names: NA

Gene position: 1168949-1170616 (Clockwise)

Preceding gene: 59711663

Following gene: 59711667

Centisome position: 40.34

GC content: 41.97

Gene sequence:

>1668_bases
ATGAAACTAAATAGTCTGTGCATTGCTGTTATGCTTGCGACTTCTGCCTATGTAAATGCAGCTGAAATATATAACCAAGA
GCAAAGCTACCAAGCTGGTAGCCAAGTATCATTTAATGGGGATATATATGAAGCTAAATGGTGGGCAAATCCGGGTCAAA
GTCCTGCTGATGTTGCTGCAAATCCATGGGATACACCTTGGGAGCTTATCTCAGAAGGAGATGGTGTAACGCCTCCGCCA
CCCGTTGTTGATCCTGAAGAGCCTGAGGTTGAATTACCTGAAGGCGACTATCCTCAATATAAAGAGGGTGAAAAATACAA
AGGTGGTGATGTTGTTCAAAACGAAAATCAATTATACCGTTGTAAAACAGGCGTTACAGCTACTTGGTGTTCAGGAGCCG
CTTGGGCCTATGCTCCAGGAACAGGTACTGCATGGGTAGACGCTTGGGAAAAAATTACTGAAGATGATCTTGTTCCACCA
GTAGACCCAGAAATTCCACCAGTTGATCCTGAAGTTCCTCCAACGGGCGGAGACTATAAAGTAAATCAATCTGCATTGGA
TGCTAAAGAAGCGGAATTAACAAATTTTGATGTAATGAATGATGTAAAAGCATCCATTGCCACATTAGATAATGAATCTG
TTGAACGCATTATTCCTGGTGCAGAATCAAACCCTGAGAACGTAAAACGTATTGAGCAAATTGTTTCTGAAAGTACATGG
AATTTCTTATTCCCTAAGCGTGCTCCTGAATATACATACCGTCATTTCTTGCAGGCAACGGGTAAATTCCCAGCTTTCTG
TGGTACTTATGAAGATGGACGTGACTCTGATGCTATCTGTAAAAAGTCACTAGCAACTATGTTTGCTCATTTTACTCAAG
AGACCGGTGGACATACTGCTCATTGGGAAGTGCCTGAGTGGCGTCAAGGTTTAGTTCATGTTCGTGAGATGGGCTGGACT
GAAGAAATGCGTGGTGGCTATAACGGTGAATGTAATCCTGACGTATGGCAGGGGAAAACATGGCCATGTGGTACGTTTGA
TAACGGCGAATTTAAATCATACTTTGGCCGTGGAGCGAAGCAGTTAAGTTATAACTACAACTACGGACCGTTTTCTGATG
CGATGTTTGGTACCGTTCGAACTTTGTTAGATAACCCTGAAATGGTGGCAGATACGTGGTTAAACCTGTCTTCAGCGGTC
TTTTTCTTTGTTTATCCACAACCACCAAAGCCAAGCATGTTGCATGTTATTGATGGAACATGGCAACCAAACCAAGCAGA
TTTAAATAATGGCTTAGTTCCTGGGTTTGGTGTAACGACTCAGATCATTAATGGTGGTGTTGAGTGTGGTGGCAGTATTG
AAGTAGCACAATCGTTAAATCGTATTAAATATTACAAAGAAATGGCAAATTACCTAGGTGTGCCAATTGAAGATACCGAA
ATACTTGGTTGTAAAGGCATGAAGCAGTTTGATGCTGCAGGCGCTGGAGCGGTTAATATTTACTGGGAGCAAGATTGGGG
ATGGTCAAGTGAAACGCCAGATGGTAAAGCTTACGCATGTCAATTAGTAGGTTATCAAACACCTCATTCGGCATTTAAGT
TCCGCGATTATACTAAATGTGTAGAAGCGAACTTCCCGAATATTGAAGTAATTAATGATGTAGATTAA

Upstream 100 bases:

>100_bases
TTTATGTCGTCTTATTAGTGATAATAAATTAAATTTCCCTGCTATATTCCGATCAGTCGGTTACCGGCTAAATCTAATAA
TAAGTAAATAAGGGAATTTT

Downstream 100 bases:

>100_bases
TTAGTTAAATAAAGATAAACACCTTCCAGATGATTTTGGAAGGTGTTTTTTATTTTTGCAATATAAATAACCATTTTTAT
ATTGTGGTTATTGAGAGAGT

Product: basic endochitinase

Products: beta-D-Gal-(1->3)-D-GlcNAc; beta-D-Gal-(1->4)-D-Glc; lacto-N-biose; lactose

Alternate protein names: Basic Endochitinase; Endochitinase

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MKLNSLCIAVMLATSAYVNAAEIYNQEQSYQAGSQVSFNGDIYEAKWWANPGQSPADVAANPWDTPWELISEGDGVTPPP
PVVDPEEPEVELPEGDYPQYKEGEKYKGGDVVQNENQLYRCKTGVTATWCSGAAWAYAPGTGTAWVDAWEKITEDDLVPP
VDPEIPPVDPEVPPTGGDYKVNQSALDAKEAELTNFDVMNDVKASIATLDNESVERIIPGAESNPENVKRIEQIVSESTW
NFLFPKRAPEYTYRHFLQATGKFPAFCGTYEDGRDSDAICKKSLATMFAHFTQETGGHTAHWEVPEWRQGLVHVREMGWT
EEMRGGYNGECNPDVWQGKTWPCGTFDNGEFKSYFGRGAKQLSYNYNYGPFSDAMFGTVRTLLDNPEMVADTWLNLSSAV
FFFVYPQPPKPSMLHVIDGTWQPNQADLNNGLVPGFGVTTQIINGGVECGGSIEVAQSLNRIKYYKEMANYLGVPIEDTE
ILGCKGMKQFDAAGAGAVNIYWEQDWGWSSETPDGKAYACQLVGYQTPHSAFKFRDYTKCVEANFPNIEVINDVD

Sequences:

>Translated_555_residues
MKLNSLCIAVMLATSAYVNAAEIYNQEQSYQAGSQVSFNGDIYEAKWWANPGQSPADVAANPWDTPWELISEGDGVTPPP
PVVDPEEPEVELPEGDYPQYKEGEKYKGGDVVQNENQLYRCKTGVTATWCSGAAWAYAPGTGTAWVDAWEKITEDDLVPP
VDPEIPPVDPEVPPTGGDYKVNQSALDAKEAELTNFDVMNDVKASIATLDNESVERIIPGAESNPENVKRIEQIVSESTW
NFLFPKRAPEYTYRHFLQATGKFPAFCGTYEDGRDSDAICKKSLATMFAHFTQETGGHTAHWEVPEWRQGLVHVREMGWT
EEMRGGYNGECNPDVWQGKTWPCGTFDNGEFKSYFGRGAKQLSYNYNYGPFSDAMFGTVRTLLDNPEMVADTWLNLSSAV
FFFVYPQPPKPSMLHVIDGTWQPNQADLNNGLVPGFGVTTQIINGGVECGGSIEVAQSLNRIKYYKEMANYLGVPIEDTE
ILGCKGMKQFDAAGAGAVNIYWEQDWGWSSETPDGKAYACQLVGYQTPHSAFKFRDYTKCVEANFPNIEVINDVD
>Mature_555_residues
MKLNSLCIAVMLATSAYVNAAEIYNQEQSYQAGSQVSFNGDIYEAKWWANPGQSPADVAANPWDTPWELISEGDGVTPPP
PVVDPEEPEVELPEGDYPQYKEGEKYKGGDVVQNENQLYRCKTGVTATWCSGAAWAYAPGTGTAWVDAWEKITEDDLVPP
VDPEIPPVDPEVPPTGGDYKVNQSALDAKEAELTNFDVMNDVKASIATLDNESVERIIPGAESNPENVKRIEQIVSESTW
NFLFPKRAPEYTYRHFLQATGKFPAFCGTYEDGRDSDAICKKSLATMFAHFTQETGGHTAHWEVPEWRQGLVHVREMGWT
EEMRGGYNGECNPDVWQGKTWPCGTFDNGEFKSYFGRGAKQLSYNYNYGPFSDAMFGTVRTLLDNPEMVADTWLNLSSAV
FFFVYPQPPKPSMLHVIDGTWQPNQADLNNGLVPGFGVTTQIINGGVECGGSIEVAQSLNRIKYYKEMANYLGVPIEDTE
ILGCKGMKQFDAAGAGAVNIYWEQDWGWSSETPDGKAYACQLVGYQTPHSAFKFRDYTKCVEANFPNIEVINDVD

Specific function: Unknown

COG id: COG3979

COG function: function code R; Uncharacterized protein contain chitin-binding domain type 3

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Caenorhabditis elegans, GI17563934, Length=347, Percent_Identity=29.3948126801153, Blast_Score=110, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71983294, Length=281, Percent_Identity=31.6725978647687, Blast_Score=82, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI71983301, Length=281, Percent_Identity=31.6725978647687, Blast_Score=82, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17564732, Length=280, Percent_Identity=31.0714285714286, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17563086, Length=280, Percent_Identity=31.0714285714286, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17565884, Length=189, Percent_Identity=35.4497354497354, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.2.1.14

Molecular weight: Translated: 61618; Mature: 61618

Theoretical pI: Translated: 4.16; Mature: 4.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNSLCIAVMLATSAYVNAAEIYNQEQSYQAGSQVSFNGDIYEAKWWANPGQSPADVAA
CCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEECCCEEEEEECCCCCCCHHCCCC
NPWDTPWELISEGDGVTPPPPVVDPEEPEVELPEGDYPQYKEGEKYKGGDVVQNENQLYR
CCCCCHHHHHHCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCEEECCCCEEE
CKTGVTATWCSGAAWAYAPGTGTAWVDAWEKITEDDLVPPVDPEIPPVDPEVPPTGGDYK
EECCCEEEECCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEE
VNQSALDAKEAELTNFDVMNDVKASIATLDNESVERIIPGAESNPENVKRIEQIVSESTW
ECHHHHCHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHHHHHCCCC
NFLFPKRAPEYTYRHFLQATGKFPAFCGTYEDGRDSDAICKKSLATMFAHFTQETGGHTA
CEECCCCCCHHHHHHHHHHCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEE
HWEVPEWRQGLVHVREMGWTEEMRGGYNGECNPDVWQGKTWPCGTFDNGEFKSYFGRGAK
ECCCCHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCC
QLSYNYNYGPFSDAMFGTVRTLLDNPEMVADTWLNLSSAVFFFVYPQPPKPSMLHVIDGT
EEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEEECCCCCCCEEEEECCC
WQPNQADLNNGLVPGFGVTTQIINGGVECGGSIEVAQSLNRIKYYKEMANYLGVPIEDTE
CCCCHHCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
ILGCKGMKQFDAAGAGAVNIYWEQDWGWSSETPDGKAYACQLVGYQTPHSAFKFRDYTKC
EECCCCCHHHHCCCCCEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHH
VEANFPNIEVINDVD
HHCCCCCEEEECCCC
>Mature Secondary Structure
MKLNSLCIAVMLATSAYVNAAEIYNQEQSYQAGSQVSFNGDIYEAKWWANPGQSPADVAA
CCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEECCCEEEEEECCCCCCCHHCCCC
NPWDTPWELISEGDGVTPPPPVVDPEEPEVELPEGDYPQYKEGEKYKGGDVVQNENQLYR
CCCCCHHHHHHCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCEEECCCCEEE
CKTGVTATWCSGAAWAYAPGTGTAWVDAWEKITEDDLVPPVDPEIPPVDPEVPPTGGDYK
EECCCEEEECCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEE
VNQSALDAKEAELTNFDVMNDVKASIATLDNESVERIIPGAESNPENVKRIEQIVSESTW
ECHHHHCHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHHHHHCCCC
NFLFPKRAPEYTYRHFLQATGKFPAFCGTYEDGRDSDAICKKSLATMFAHFTQETGGHTA
CEECCCCCCHHHHHHHHHHCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEE
HWEVPEWRQGLVHVREMGWTEEMRGGYNGECNPDVWQGKTWPCGTFDNGEFKSYFGRGAK
ECCCCHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCC
QLSYNYNYGPFSDAMFGTVRTLLDNPEMVADTWLNLSSAVFFFVYPQPPKPSMLHVIDGT
EEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEEECCCCCCCEEEEECCC
WQPNQADLNNGLVPGFGVTTQIINGGVECGGSIEVAQSLNRIKYYKEMANYLGVPIEDTE
CCCCHHCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
ILGCKGMKQFDAAGAGAVNIYWEQDWGWSSETPDGKAYACQLVGYQTPHSAFKFRDYTKC
EECCCCCHHHHCCCCCEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHH
VEANFPNIEVINDVD
HHCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: beta-D-Gal-(1->3)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-D-Glc; H2O; lacto-N-tetraose

Specific reaction: (1) beta-D-Gal-(1->3)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-D-Glc + H2O = beta-D-Gal-(1->3)-D-GlcNAc + beta-D-Gal-(1->4)-D-Glc; (2) lacto-N-tetraose + H2O = lacto-N-biose + lactose

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA