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The map label for this gene is pepT [H]

Identifier: 59711667

GI number: 59711667

Start: 1170782

End: 1172011

Strand: Direct

Name: pepT [H]

Synonym: VF_1060

Alternate gene names: 59711667

Gene position: 1170782-1172011 (Clockwise)

Preceding gene: 59711666

Following gene: 59711668

Centisome position: 40.41

GC content: 39.67

Gene sequence:

>1230_bases
ATGAATGTAGTAGAACGTTTCTTAGGTTACACAAAGATTAATACGACAACAAACCAAGCTAATGGGGCAGCGGGCATTAT
GCCTTCAAGTGAAGGGCAATATGAATTGGCTTGTTTGGTTAAAAAAGAATTAGAAGCGCTAGGTTGTACAGATATTCTTC
TTCGTGATACCGCTATTCTTACGGCAACTCTGCCATCTAATACCGAAAAAAAATTACCAACCGTTGCTTTCTTTGGGCAC
CTTGATACCAGCTCTGAACGTACAGAAGACACCCATGCCGTAGTGGTTCATTATAAAGGTGGCGATATTCCTCTCGCAAC
AGGACCTGTTTTATCTTTAGCTGAATATCCAGAACTAACTAATTATATTGGTCAAGACATCATTGTAAGTGACGGTCAAT
CATTATTAGGCGCAGATGATAAAGCAGCCATTGCCGCTATCTTAAATGCGTTACAGTTTTTTAAAGACAACCCAGATGTT
GAACATGGAATGGTTAAGGTTGGTTTTGTTCCTGATGAAGAACAAGGTCTTTTAGGGGCGAATGCTTTTGATGTTGACGG
TTTTGGCGCTGATTTTGCGTACACTTTGGATTGCTGTGGTATTGGTGAGTTTGTATGTGAAAACTGGAATGCAGGTAATG
CGGTTGTTACGTTTACAGGGCAGTCTGCACACCCTATGAACTCTAAAGGTAATTTGATTAACTCTTTGCTACTGGCTAAT
TCATTCATGTCCCGTTTTCCTGAGAAAGAAACTCCTGAGCATACAGAAGGAAGAGAAGGCTATTACTGGATGAAGAAGTT
GGCGGGAAATTCTGCCAAAACAGTGCTTAATATGGATATCAGAGATTTCACTGAATCAGGATATATGTATCGTAAAGCCT
TTATTGCTGAACAAGTTCGAATGTTCAATGAAGAAAACAATAACCGTGCAACTCTTGAATTAAGTGATCGTTACAGTAAC
GTTCATAACTTCTTAAAAGGCTCCATGCTGCCAGTTGATCTTGCAATGGAAGCATATAAGGAAAATGACATTGAACCTAA
AGTTATACCGATGCGCGGCGGCTATGATGGTGCAGTTTTGTCTGAAAAAGGTTTGCCATGCCCTAACATTTTTACCGGCG
CACATAACTTCCACTCTATATTTGAATACCTTCCTGTCCCATCTTTAATTGCTGCAAGTAATGTTGTGAAAACAATCATT
ATTAACTTAACGAAACATGAAGAGGTGTAG

Upstream 100 bases:

>100_bases
AATAACCATTTTTATATTGTGGTTATTGAGAGAGTAGTCACTTAAATAAGATGGAGAGTTTGTAATATCGTTGTTTTATC
AATCGTTTTGGATTAAAGCA

Downstream 100 bases:

>100_bases
TCATGGCTTCGTTATTACTTGTTCTTATCGTTGTTGTTATTGCGGCAAGGATGATTTTAAAGGGGTATAAAGCTGAACCT
ACTTTATTAATGTCAGGGAC

Product: peptidase T

Products: NA

Alternate protein names: Aminotripeptidase; Tripeptidase; Tripeptide aminopeptidase [H]

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MNVVERFLGYTKINTTTNQANGAAGIMPSSEGQYELACLVKKELEALGCTDILLRDTAILTATLPSNTEKKLPTVAFFGH
LDTSSERTEDTHAVVVHYKGGDIPLATGPVLSLAEYPELTNYIGQDIIVSDGQSLLGADDKAAIAAILNALQFFKDNPDV
EHGMVKVGFVPDEEQGLLGANAFDVDGFGADFAYTLDCCGIGEFVCENWNAGNAVVTFTGQSAHPMNSKGNLINSLLLAN
SFMSRFPEKETPEHTEGREGYYWMKKLAGNSAKTVLNMDIRDFTESGYMYRKAFIAEQVRMFNEENNNRATLELSDRYSN
VHNFLKGSMLPVDLAMEAYKENDIEPKVIPMRGGYDGAVLSEKGLPCPNIFTGAHNFHSIFEYLPVPSLIAASNVVKTII
INLTKHEEV

Sequences:

>Translated_409_residues
MNVVERFLGYTKINTTTNQANGAAGIMPSSEGQYELACLVKKELEALGCTDILLRDTAILTATLPSNTEKKLPTVAFFGH
LDTSSERTEDTHAVVVHYKGGDIPLATGPVLSLAEYPELTNYIGQDIIVSDGQSLLGADDKAAIAAILNALQFFKDNPDV
EHGMVKVGFVPDEEQGLLGANAFDVDGFGADFAYTLDCCGIGEFVCENWNAGNAVVTFTGQSAHPMNSKGNLINSLLLAN
SFMSRFPEKETPEHTEGREGYYWMKKLAGNSAKTVLNMDIRDFTESGYMYRKAFIAEQVRMFNEENNNRATLELSDRYSN
VHNFLKGSMLPVDLAMEAYKENDIEPKVIPMRGGYDGAVLSEKGLPCPNIFTGAHNFHSIFEYLPVPSLIAASNVVKTII
INLTKHEEV
>Mature_409_residues
MNVVERFLGYTKINTTTNQANGAAGIMPSSEGQYELACLVKKELEALGCTDILLRDTAILTATLPSNTEKKLPTVAFFGH
LDTSSERTEDTHAVVVHYKGGDIPLATGPVLSLAEYPELTNYIGQDIIVSDGQSLLGADDKAAIAAILNALQFFKDNPDV
EHGMVKVGFVPDEEQGLLGANAFDVDGFGADFAYTLDCCGIGEFVCENWNAGNAVVTFTGQSAHPMNSKGNLINSLLLAN
SFMSRFPEKETPEHTEGREGYYWMKKLAGNSAKTVLNMDIRDFTESGYMYRKAFIAEQVRMFNEENNNRATLELSDRYSN
VHNFLKGSMLPVDLAMEAYKENDIEPKVIPMRGGYDGAVLSEKGLPCPNIFTGAHNFHSIFEYLPVPSLIAASNVVKTII
INLTKHEEV

Specific function: Cleaves the N-terminal amino acid of tripeptides [H]

COG id: COG2195

COG function: function code E; Di- and tripeptidases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20B family [H]

Homologues:

Organism=Escherichia coli, GI1787372, Length=403, Percent_Identity=37.9652605459057, Blast_Score=277, Evalue=9e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR002933
- InterPro:   IPR011650
- InterPro:   IPR010161 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.4.11.4 [H]

Molecular weight: Translated: 44791; Mature: 44791

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS00758 ARGE_DAPE_CPG2_1 ; PS00759 ARGE_DAPE_CPG2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVVERFLGYTKINTTTNQANGAAGIMPSSEGQYELACLVKKELEALGCTDILLRDTAIL
CCHHHHHHCCEEECCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHCCCHHHEEECCEEE
TATLPSNTEKKLPTVAFFGHLDTSSERTEDTHAVVVHYKGGDIPLATGPVLSLAEYPELT
EEECCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHCHHHH
NYIGQDIIVSDGQSLLGADDKAAIAAILNALQFFKDNPDVEHGMVKVGFVPDEEQGLLGA
HHCCCCEEEECCCHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCC
NAFDVDGFGADFAYTLDCCGIGEFVCENWNAGNAVVTFTGQSAHPMNSKGNLINSLLLAN
CEECCCCCCCCCEEEEECCCCCHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHH
SFMSRFPEKETPEHTEGREGYYWMKKLAGNSAKTVLNMDIRDFTESGYMYRKAFIAEQVR
HHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCEEEECCHHHHHHCCHHHHHHHHHHHHH
MFNEENNNRATLELSDRYSNVHNFLKGSMLPVDLAMEAYKENDIEPKVIPMRGGYDGAVL
HHCCCCCCEEEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCEE
SEKGLPCPNIFTGAHNFHSIFEYLPVPSLIAASNVVKTIIINLTKHEEV
CCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHEEECCCCCC
>Mature Secondary Structure
MNVVERFLGYTKINTTTNQANGAAGIMPSSEGQYELACLVKKELEALGCTDILLRDTAIL
CCHHHHHHCCEEECCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHCCCHHHEEECCEEE
TATLPSNTEKKLPTVAFFGHLDTSSERTEDTHAVVVHYKGGDIPLATGPVLSLAEYPELT
EEECCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHCHHHH
NYIGQDIIVSDGQSLLGADDKAAIAAILNALQFFKDNPDVEHGMVKVGFVPDEEQGLLGA
HHCCCCEEEECCCHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCC
NAFDVDGFGADFAYTLDCCGIGEFVCENWNAGNAVVTFTGQSAHPMNSKGNLINSLLLAN
CEECCCCCCCCCEEEEECCCCCHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHH
SFMSRFPEKETPEHTEGREGYYWMKKLAGNSAKTVLNMDIRDFTESGYMYRKAFIAEQVR
HHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCEEEECCHHHHHHCCHHHHHHHHHHHHH
MFNEENNNRATLELSDRYSNVHNFLKGSMLPVDLAMEAYKENDIEPKVIPMRGGYDGAVL
HHCCCCCCEEEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCEE
SEKGLPCPNIFTGAHNFHSIFEYLPVPSLIAASNVVKTIIINLTKHEEV
CCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA