| Definition | Vibrio fischeri ES114 chromosome I, complete genome. |
|---|---|
| Accession | NC_006840 |
| Length | 2,897,536 |
Click here to switch to the map view.
The map label for this gene is topA [H]
Identifier: 59711658
GI number: 59711658
Start: 1158157
End: 1160787
Strand: Direct
Name: topA [H]
Synonym: VF_1051
Alternate gene names: 59711658
Gene position: 1158157-1160787 (Clockwise)
Preceding gene: 59711655
Following gene: 59711659
Centisome position: 39.97
GC content: 41.77
Gene sequence:
>2631_bases ATGGGTAAATCTCTAGTTATCGTGGAGTCACCAGCCAAAGCGAAAACCATCAATAAATACCTTGGCAAAGACTTCATCGT AAAATCCAGTGTAGGTCACGTACGTGATTTGCCTATGGGTTCGACAAGCACTGGTAAAAAGGCAGCAGCGTCGACCAAAG GGTTAAGTGTTGAAGAAAAAGCACGAATTAAAAAAGAAAGAGATAAAAAGAACCTAATTAATAAAATGGGTATTGACCCA TACAATGATTGGGCAGCTAACTACCAAATCCTTCCGGGTAAAGAAAAAGTAGTAGCAGAATTACAAAAATTAGCAGAAAG CGCAGATACCGTTTATCTCGCAACCGATTTGGACCGTGAAGGGGAAGCTATTGCGTGGCACCTTCGCGAGATCATCGGTG GTGATGATTCACGCTTCAAACGTGTGGTTTTTAACGAAATCACGAAAAATGCGATTCAACAGGCATTCGAAACTCCAGGT GAACTAAGCATGCCTGGCGTAAATGCACAACAAGCTCGTCGTTTCTTAGACCGTGTTGTTGGCTTTATGGCATCTCCATT ATTGTGGAAGAAAGTGGCTCGTGGTTTATCTGCTGGCCGTGTGCAATCGGTAGCAGTGAAAGTATTGGTAGAGCGTGAGC GTGAAATCAATGCTTTTATTCCCGAAGAGTTTTGGGATATTCACGCAGACACGTTAACAGCAGATAAAGAAAACTTCCGT CTGCAAGTAGCACAACGTAACGGTTCAGCATTTAAGCCGCTTAATCAAGCAGATACAGAAGCAGCTGTAAGCATTCTTCA AAATGCAGAATACGAAGTGTGTAAACGTGAAGATCGCCCAACGAAAAGTAAGCCATCTGCACCATTTATTACATCAACAC TGCAACAGGCGGCAAGTACACGTCTAGGTTATGGTGTTAAAAAGACGATGATGTTAGCTCAGCGCCTCTATGAGGGCGGT TACATCACTTATATGCGTACTGACTCAACTAACTTAAGTAAAGAAGCTGTAGAGGCGTTACGTGAGTTTATTGGTTCTGA GTATGGCGATAGCTATTTACCAGCAGCTCCTATTGTTTATGGTAGCAAAGAAGGTGCTCAAGAAGCCCACGAAGCGATTC GTCCTTCAAGCGTAGAAGTAAAAGCGGACGACCTACAAGGTATGGAAGCTGATGCGCATAAACTGTACAACTTAATTTGG AATCAATTCGTAGCATGTCAAATGACACCTGCGCAATATGATTCAACAACATTGAGCGTAAAAGCAGACGAATTCACACT AAAAGCTAAAGGACGTATCCTGAAGTTTGATGGTTGGACGCGTGTACAGCGCCCAATGGGTAAAAATGAAGATCAACTGC TTCCTGCTGTGCAACTTGGCGATAAGCTAAAGTTAGAAAAACTGGATCCTAAGCAGCACTTTACTAAGCCACCAGCTCGT TTCACAGAAGCGGCATTAGTTAAAGAACTAGAGAAAAAAGGCATTGGTCGTCCATCGACTTACGCATCAATTATTTCAAC GATTCAAGATCGTGGTTATGTTCGCATTGATAACCGTCGTTTCTACGCTGAAAAAATGGGTGAAATCGTAACTGATCGTC TAAATCAATCATTTGATGATTTAATGGATTACGACTTTACAGCTCGCATGGAAGGCAATCTAGATAGAATTGCAGAAGGC GAAAAAGATTGGAAAGATGTGCTTAATACATTCTTCCAAGATTTTACTGCAGACATTGCAAAAGCAGAGCTGGATGAATC TGAAGGCGGTATGTCTCCAAATAACATCGTTGAGACAGATATTAAATGTCCAACATGTGGTCGCAATATGGGTATCCGTA CTGCATCTACAGGTGTTTTCCTTGGTTGTTCAGGTTATGCATTGCCACCAAAAGAGCGTTGTAAAACAACCATTAACTTA GGTAGTGAAGAGGGCATTGTAAATGTTCTTGAAGAAGACGTAGAAACAGCTGCATTACGTGCGAAGAAACGTTGTCCTAA GTGTGAAACGGCGATGGATCCTTATCTAATTGACCAAGAGCGTAAGATTCACGTTTGTGGTAATAACCCGAACTGTGAAG GTTATGTGGTTGAAAAGGGTGAATTCCAACTTAAAGGATACGATGGTCCTGTTGTTGAATGTGACAAGTGTGGTTCTGAT ATGGAACTGAAAAACGGTCGCTTTGGCAAATACATGGACTGTACGAGTGAAACATGTAAAAACACTCGTAAGATCTTAAA GAATGGTGAAGTTGCTCCACCAAAAGAAGATCCTGTACATCTACCAGAATTAGTATGTGAAAAATCAGATGCTTATTTTG TATTGCGTGATGGTGCGTCAGGTCTATTCTTAGCTGCGAGCACTTTCCCTAAATCACGTGAAACACGTGCACCATTAGTG GAAGAGTTAGTTCGATTTAAGGATCGTTTGTCGCCTAAGTTCTTATACTTAACAGAAGCGCCAACACATGACCCTGATGG TAAACCTGCGGTAGTTCGTTTTAGTCGTAAAACAAAAGAGAACTATGTTCGTTCAGAAGTGGATGGCAAGCCAACAGGTT GGACTGGTTTATATATTGATGGAAAGTGGGCTATTACCGATAAGCGTAAGAAGCCAAAAGCTGAAAAATAA
Upstream 100 bases:
>100_bases CCTCAATAGTAATATATAGATAAACATTAGATTTTAGTTGTGGATTAAACGAAAATCGGCAGCTAAGTAAATTTCAACAC GAGGACGTTAGTGAGACATT
Downstream 100 bases:
>100_bases TCAATAATAAGATATCAAATGAAAGAGCTGCAGAAATGCGGCTCTTTTTTTACGTAATATTTGTCACAAATAGTCATATT TTTAATTATTTCAAAATAAT
Product: DNA topoisomerase I
Products: NA
Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]
Number of amino acids: Translated: 876; Mature: 875
Protein sequence:
>876_residues MGKSLVIVESPAKAKTINKYLGKDFIVKSSVGHVRDLPMGSTSTGKKAAASTKGLSVEEKARIKKERDKKNLINKMGIDP YNDWAANYQILPGKEKVVAELQKLAESADTVYLATDLDREGEAIAWHLREIIGGDDSRFKRVVFNEITKNAIQQAFETPG ELSMPGVNAQQARRFLDRVVGFMASPLLWKKVARGLSAGRVQSVAVKVLVEREREINAFIPEEFWDIHADTLTADKENFR LQVAQRNGSAFKPLNQADTEAAVSILQNAEYEVCKREDRPTKSKPSAPFITSTLQQAASTRLGYGVKKTMMLAQRLYEGG YITYMRTDSTNLSKEAVEALREFIGSEYGDSYLPAAPIVYGSKEGAQEAHEAIRPSSVEVKADDLQGMEADAHKLYNLIW NQFVACQMTPAQYDSTTLSVKADEFTLKAKGRILKFDGWTRVQRPMGKNEDQLLPAVQLGDKLKLEKLDPKQHFTKPPAR FTEAALVKELEKKGIGRPSTYASIISTIQDRGYVRIDNRRFYAEKMGEIVTDRLNQSFDDLMDYDFTARMEGNLDRIAEG EKDWKDVLNTFFQDFTADIAKAELDESEGGMSPNNIVETDIKCPTCGRNMGIRTASTGVFLGCSGYALPPKERCKTTINL GSEEGIVNVLEEDVETAALRAKKRCPKCETAMDPYLIDQERKIHVCGNNPNCEGYVVEKGEFQLKGYDGPVVECDKCGSD MELKNGRFGKYMDCTSETCKNTRKILKNGEVAPPKEDPVHLPELVCEKSDAYFVLRDGASGLFLAASTFPKSRETRAPLV EELVRFKDRLSPKFLYLTEAPTHDPDGKPAVVRFSRKTKENYVRSEVDGKPTGWTGLYIDGKWAITDKRKKPKAEK
Sequences:
>Translated_876_residues MGKSLVIVESPAKAKTINKYLGKDFIVKSSVGHVRDLPMGSTSTGKKAAASTKGLSVEEKARIKKERDKKNLINKMGIDP YNDWAANYQILPGKEKVVAELQKLAESADTVYLATDLDREGEAIAWHLREIIGGDDSRFKRVVFNEITKNAIQQAFETPG ELSMPGVNAQQARRFLDRVVGFMASPLLWKKVARGLSAGRVQSVAVKVLVEREREINAFIPEEFWDIHADTLTADKENFR LQVAQRNGSAFKPLNQADTEAAVSILQNAEYEVCKREDRPTKSKPSAPFITSTLQQAASTRLGYGVKKTMMLAQRLYEGG YITYMRTDSTNLSKEAVEALREFIGSEYGDSYLPAAPIVYGSKEGAQEAHEAIRPSSVEVKADDLQGMEADAHKLYNLIW NQFVACQMTPAQYDSTTLSVKADEFTLKAKGRILKFDGWTRVQRPMGKNEDQLLPAVQLGDKLKLEKLDPKQHFTKPPAR FTEAALVKELEKKGIGRPSTYASIISTIQDRGYVRIDNRRFYAEKMGEIVTDRLNQSFDDLMDYDFTARMEGNLDRIAEG EKDWKDVLNTFFQDFTADIAKAELDESEGGMSPNNIVETDIKCPTCGRNMGIRTASTGVFLGCSGYALPPKERCKTTINL GSEEGIVNVLEEDVETAALRAKKRCPKCETAMDPYLIDQERKIHVCGNNPNCEGYVVEKGEFQLKGYDGPVVECDKCGSD MELKNGRFGKYMDCTSETCKNTRKILKNGEVAPPKEDPVHLPELVCEKSDAYFVLRDGASGLFLAASTFPKSRETRAPLV EELVRFKDRLSPKFLYLTEAPTHDPDGKPAVVRFSRKTKENYVRSEVDGKPTGWTGLYIDGKWAITDKRKKPKAEK >Mature_875_residues GKSLVIVESPAKAKTINKYLGKDFIVKSSVGHVRDLPMGSTSTGKKAAASTKGLSVEEKARIKKERDKKNLINKMGIDPY NDWAANYQILPGKEKVVAELQKLAESADTVYLATDLDREGEAIAWHLREIIGGDDSRFKRVVFNEITKNAIQQAFETPGE LSMPGVNAQQARRFLDRVVGFMASPLLWKKVARGLSAGRVQSVAVKVLVEREREINAFIPEEFWDIHADTLTADKENFRL QVAQRNGSAFKPLNQADTEAAVSILQNAEYEVCKREDRPTKSKPSAPFITSTLQQAASTRLGYGVKKTMMLAQRLYEGGY ITYMRTDSTNLSKEAVEALREFIGSEYGDSYLPAAPIVYGSKEGAQEAHEAIRPSSVEVKADDLQGMEADAHKLYNLIWN QFVACQMTPAQYDSTTLSVKADEFTLKAKGRILKFDGWTRVQRPMGKNEDQLLPAVQLGDKLKLEKLDPKQHFTKPPARF TEAALVKELEKKGIGRPSTYASIISTIQDRGYVRIDNRRFYAEKMGEIVTDRLNQSFDDLMDYDFTARMEGNLDRIAEGE KDWKDVLNTFFQDFTADIAKAELDESEGGMSPNNIVETDIKCPTCGRNMGIRTASTGVFLGCSGYALPPKERCKTTINLG SEEGIVNVLEEDVETAALRAKKRCPKCETAMDPYLIDQERKIHVCGNNPNCEGYVVEKGEFQLKGYDGPVVECDKCGSDM ELKNGRFGKYMDCTSETCKNTRKILKNGEVAPPKEDPVHLPELVCEKSDAYFVLRDGASGLFLAASTFPKSRETRAPLVE ELVRFKDRLSPKFLYLTEAPTHDPDGKPAVVRFSRKTKENYVRSEVDGKPTGWTGLYIDGKWAITDKRKKPKAEK
Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]
COG id: COG0550
COG function: function code L; Topoisomerase IA
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 Toprim domain [H]
Homologues:
Organism=Homo sapiens, GI10835218, Length=614, Percent_Identity=26.0586319218241, Blast_Score=148, Evalue=2e-35, Organism=Homo sapiens, GI4507635, Length=619, Percent_Identity=25.0403877221325, Blast_Score=133, Evalue=6e-31, Organism=Escherichia coli, GI1787529, Length=865, Percent_Identity=70.5202312138728, Blast_Score=1288, Evalue=0.0, Organism=Escherichia coli, GI1788061, Length=537, Percent_Identity=23.8361266294227, Blast_Score=106, Evalue=5e-24, Organism=Caenorhabditis elegans, GI32563869, Length=600, Percent_Identity=25.1666666666667, Blast_Score=127, Evalue=2e-29, Organism=Caenorhabditis elegans, GI17555378, Length=524, Percent_Identity=24.0458015267176, Blast_Score=111, Evalue=2e-24, Organism=Saccharomyces cerevisiae, GI6323263, Length=562, Percent_Identity=24.3772241992883, Blast_Score=102, Evalue=2e-22, Organism=Drosophila melanogaster, GI24585251, Length=627, Percent_Identity=24.5614035087719, Blast_Score=133, Evalue=5e-31, Organism=Drosophila melanogaster, GI24640096, Length=501, Percent_Identity=24.9500998003992, Blast_Score=118, Evalue=2e-26,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR003601 - InterPro: IPR013497 - InterPro: IPR013824 - InterPro: IPR013825 - InterPro: IPR000380 - InterPro: IPR003602 - InterPro: IPR013498 - InterPro: IPR005733 - InterPro: IPR013263 - InterPro: IPR006171 [H]
Pfam domain/function: PF08272 Topo_Zn_Ribbon; PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]
EC number: =5.99.1.2 [H]
Molecular weight: Translated: 97909; Mature: 97778
Theoretical pI: Translated: 7.89; Mature: 7.89
Prosite motif: PS00396 TOPOISOMERASE_I_PROK
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.7 %Cys (Translated Protein) 2.2 %Met (Translated Protein) 3.9 %Cys+Met (Translated Protein) 1.7 %Cys (Mature Protein) 2.1 %Met (Mature Protein) 3.8 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MGKSLVIVESPAKAKTINKYLGKDFIVKSSVGHVRDLPMGSTSTGKKAAASTKGLSVEEK CCCCEEEEECCCHHHHHHHHHCCCEEEECCCCCHHCCCCCCCCCCCHHHCCCCCCCHHHH ARIKKERDKKNLINKMGIDPYNDWAANYQILPGKEKVVAELQKLAESADTVYLATDLDRE HHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHCCCEEEEEECCCCC GEAIAWHLREIIGGDDSRFKRVVFNEITKNAIQQAFETPGELSMPGVNAQQARRFLDRVV CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH GFMASPLLWKKVARGLSAGRVQSVAVKVLVEREREINAFIPEEFWDIHADTLTADKENFR HHHHCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHEECCCCCCE LQVAQRNGSAFKPLNQADTEAAVSILQNAEYEVCKREDRPTKSKPSAPFITSTLQQAAST EEEEECCCCCCCCCCHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH RLGYGVKKTMMLAQRLYEGGYITYMRTDSTNLSKEAVEALREFIGSEYGDSYLPAAPIVY HHCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEE GSKEGAQEAHEAIRPSSVEVKADDLQGMEADAHKLYNLIWNQFVACQMTPAQYDSTTLSV CCCCCHHHHHHHCCCCCEEEEHHHCCCCCHHHHHHHHHHHHHHEEEEECCCCCCCEEEEE KADEFTLKAKGRILKFDGWTRVQRPMGKNEDQLLPAVQLGDKLKLEKLDPKQHFTKPPAR EECCEEEEECCCEEEECCCHHHHCCCCCCHHHCCHHHHCCCCEEECCCCCHHHCCCCCHH FTEAALVKELEKKGIGRPSTYASIISTIQDRGYVRIDNRRFYAEKMGEIVTDRLNQSFDD HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEECCCEEHHHHHHHHHHHHHHCCHHH LMDYDFTARMEGNLDRIAEGEKDWKDVLNTFFQDFTADIAKAELDESEGGMSPNNIVETD HHCCCCEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECC IKCPTCGRNMGIRTASTGVFLGCSGYALPPKERCKTTINLGSEEGIVNVLEEDVETAALR CCCCCCCCCCCCEEECCCEEEECCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH AKKRCPKCETAMDPYLIDQERKIHVCGNNPNCEGYVVEKGEFQLKGYDGPVVECDKCGSD HHHCCCCCHHCCCCEEECCCCEEEEECCCCCCCEEEEECCEEEEECCCCCEEECCCCCCC MELKNGRFGKYMDCTSETCKNTRKILKNGEVAPPKEDPVHLPELVCEKSDAYFVLRDGAS CCCCCCCCCCEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCEEEEECCCC GLFLAASTFPKSRETRAPLVEELVRFKDRLSPKFLYLTEAPTHDPDGKPAVVRFSRKTKE CEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEHHHHHH NYVRSEVDGKPTGWTGLYIDGKWAITDKRKKPKAEK HHHHHHCCCCCCCEEEEEECCEEEECCCCCCCCCCC >Mature Secondary Structure GKSLVIVESPAKAKTINKYLGKDFIVKSSVGHVRDLPMGSTSTGKKAAASTKGLSVEEK CCCEEEEECCCHHHHHHHHHCCCEEEECCCCCHHCCCCCCCCCCCHHHCCCCCCCHHHH ARIKKERDKKNLINKMGIDPYNDWAANYQILPGKEKVVAELQKLAESADTVYLATDLDRE HHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHCCCEEEEEECCCCC GEAIAWHLREIIGGDDSRFKRVVFNEITKNAIQQAFETPGELSMPGVNAQQARRFLDRVV CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH GFMASPLLWKKVARGLSAGRVQSVAVKVLVEREREINAFIPEEFWDIHADTLTADKENFR HHHHCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHEECCCCCCE LQVAQRNGSAFKPLNQADTEAAVSILQNAEYEVCKREDRPTKSKPSAPFITSTLQQAAST EEEEECCCCCCCCCCHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH RLGYGVKKTMMLAQRLYEGGYITYMRTDSTNLSKEAVEALREFIGSEYGDSYLPAAPIVY HHCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEE GSKEGAQEAHEAIRPSSVEVKADDLQGMEADAHKLYNLIWNQFVACQMTPAQYDSTTLSV CCCCCHHHHHHHCCCCCEEEEHHHCCCCCHHHHHHHHHHHHHHEEEEECCCCCCCEEEEE KADEFTLKAKGRILKFDGWTRVQRPMGKNEDQLLPAVQLGDKLKLEKLDPKQHFTKPPAR EECCEEEEECCCEEEECCCHHHHCCCCCCHHHCCHHHHCCCCEEECCCCCHHHCCCCCHH FTEAALVKELEKKGIGRPSTYASIISTIQDRGYVRIDNRRFYAEKMGEIVTDRLNQSFDD HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEECCCEEHHHHHHHHHHHHHHCCHHH LMDYDFTARMEGNLDRIAEGEKDWKDVLNTFFQDFTADIAKAELDESEGGMSPNNIVETD HHCCCCEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECC IKCPTCGRNMGIRTASTGVFLGCSGYALPPKERCKTTINLGSEEGIVNVLEEDVETAALR CCCCCCCCCCCCEEECCCEEEECCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH AKKRCPKCETAMDPYLIDQERKIHVCGNNPNCEGYVVEKGEFQLKGYDGPVVECDKCGSD HHHCCCCCHHCCCCEEECCCCEEEEECCCCCCCEEEEECCEEEEECCCCCEEECCCCCCC MELKNGRFGKYMDCTSETCKNTRKILKNGEVAPPKEDPVHLPELVCEKSDAYFVLRDGAS CCCCCCCCCCEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCEEEEECCCC GLFLAASTFPKSRETRAPLVEELVRFKDRLSPKFLYLTEAPTHDPDGKPAVVRFSRKTKE CEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEHHHHHH NYVRSEVDGKPTGWTGLYIDGKWAITDKRKKPKAEK HHHHHHCCCCCCCEEEEEECCEEEECCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 10952301 [H]