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The map label for this gene is trpB [H]

Identifier: 59711635

GI number: 59711635

Start: 1132491

End: 1133681

Strand: Reverse

Name: trpB [H]

Synonym: VF_1028

Alternate gene names: 59711635

Gene position: 1133681-1132491 (Counterclockwise)

Preceding gene: 59711636

Following gene: 59711634

Centisome position: 39.13

GC content: 42.99

Gene sequence:

>1191_bases
ATGGCAAAATTAGATGCCTACTTCGGCGAATACGGTGGTCAATACGTTCCACAGATTTTAGTTCCAGCTTTAGAGCAACT
AGAACAAGCCTTTATTGATGCACAAGAAGATCCAGACTTTCGTTCTGAGTTCATGACGCTTTTACAAGAATATGCGGGTC
GTCCAACTGCATTGACGTTATGCCGCAATTTAACCAAAGGCACAAAAACAAAGCTATACCTTAAACGGGAAGATCTTCTT
CATGGTGGTGCACATAAAACCAACCAAGTACTTGGCCAAGCACTACTTGCAAAGCGTATGGGCAAAGATGAGATCATTGC
TGAAACAGGTGCAGGCCAACACGGTGTTGCCACAGCACTAGCTTGTGCACTACTTGGGTTAAAATGCCGAGTTTATATGG
GAGCTAAAGACGTAGAACGTCAAAGCCCAAATGTATTCCGTATGAAACTAATGGGTGCAGAAGTCATTCCTGTTCATTCA
GGCTCTGCGACGCTAAAAGATGCATGTAATGAAGCACTACGTGACTGGTCTGCAACCTATGAAACTGCACACTATTTATT
AGGAACAGCGGCAGGCCCTCATCCATTCCCAACCATCGTTCGTGAGTTCCAACGTATGATTGGTGAAGAAACGAAAATGC
AAATCTTAGCGCGTGAAGGTCGTTTACCTGATGCGGTTATTGCTTGCGTGGGTGGCGGTTCAAATGCTATCGGTATGTTT
GCAGATTTCATTGAAGAAGAAAGCGTAAAACTAATTGGTGTTGAGCCTGCAGGTAAAGGTATTGATACCGACCAACACGG
CGCACCACTTAAGCATGGTAAAACAGGCATTTTCTTTGGAATGAAAGCCCCATTAATGCAAGATGAACACGGACAGATTG
AAGAATCATACTCAGTTTCTGCAGGTTTAGACTTCCCATCCGTTGGTCCACAACACGCCCACCTAAATGCTATTGGTCGT
GCTGAATACGGTGCAGTAACAGATGACGAAGCATTAGATGCATTCAAAATTCTTGCAAGAAATGAAGGTATTATTCCAGC
ATTAGAATCTTCTCATGCTTTAGCATATGCATTACAACTTGCACAAGCAGAACCGGAGAAAGAACAACTATTGGTAGTAA
ACTTATCTGGCCGAGGCGATAAAGACATCTTCACAGTACATGACATTTTAAAAGAAAAGGGAGAAATCTAA

Upstream 100 bases:

>100_bases
TTAGATTTCAACTCAGGAGTAGAAAGTACTCCGGGTAAAAAAGATCATAAAAAATTAAGCGCCGTATTTAGCGCATTACG
AGACTATTAAGGAAAATATC

Downstream 100 bases:

>100_bases
TGGATCGTTATCAAGCCCTATTTGCTCAGCTAGAGAAAAAAAATCAAGGAGCCTTTGTTCCATTCGTAACTATTGGCGAT
CCAAACCCTGAGCTATCTTA

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKLDAYFGEYGGQYVPQILVPALEQLEQAFIDAQEDPDFRSEFMTLLQEYAGRPTALTLCRNLTKGTKTKLYLKREDLL
HGGAHKTNQVLGQALLAKRMGKDEIIAETGAGQHGVATALACALLGLKCRVYMGAKDVERQSPNVFRMKLMGAEVIPVHS
GSATLKDACNEALRDWSATYETAHYLLGTAAGPHPFPTIVREFQRMIGEETKMQILAREGRLPDAVIACVGGGSNAIGMF
ADFIEEESVKLIGVEPAGKGIDTDQHGAPLKHGKTGIFFGMKAPLMQDEHGQIEESYSVSAGLDFPSVGPQHAHLNAIGR
AEYGAVTDDEALDAFKILARNEGIIPALESSHALAYALQLAQAEPEKEQLLVVNLSGRGDKDIFTVHDILKEKGEI

Sequences:

>Translated_396_residues
MAKLDAYFGEYGGQYVPQILVPALEQLEQAFIDAQEDPDFRSEFMTLLQEYAGRPTALTLCRNLTKGTKTKLYLKREDLL
HGGAHKTNQVLGQALLAKRMGKDEIIAETGAGQHGVATALACALLGLKCRVYMGAKDVERQSPNVFRMKLMGAEVIPVHS
GSATLKDACNEALRDWSATYETAHYLLGTAAGPHPFPTIVREFQRMIGEETKMQILAREGRLPDAVIACVGGGSNAIGMF
ADFIEEESVKLIGVEPAGKGIDTDQHGAPLKHGKTGIFFGMKAPLMQDEHGQIEESYSVSAGLDFPSVGPQHAHLNAIGR
AEYGAVTDDEALDAFKILARNEGIIPALESSHALAYALQLAQAEPEKEQLLVVNLSGRGDKDIFTVHDILKEKGEI
>Mature_395_residues
AKLDAYFGEYGGQYVPQILVPALEQLEQAFIDAQEDPDFRSEFMTLLQEYAGRPTALTLCRNLTKGTKTKLYLKREDLLH
GGAHKTNQVLGQALLAKRMGKDEIIAETGAGQHGVATALACALLGLKCRVYMGAKDVERQSPNVFRMKLMGAEVIPVHSG
SATLKDACNEALRDWSATYETAHYLLGTAAGPHPFPTIVREFQRMIGEETKMQILAREGRLPDAVIACVGGGSNAIGMFA
DFIEEESVKLIGVEPAGKGIDTDQHGAPLKHGKTGIFFGMKAPLMQDEHGQIEESYSVSAGLDFPSVGPQHAHLNAIGRA
EYGAVTDDEALDAFKILARNEGIIPALESSHALAYALQLAQAEPEKEQLLVVNLSGRGDKDIFTVHDILKEKGEI

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=393, Percent_Identity=81.4249363867684, Blast_Score=677, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6321412, Length=389, Percent_Identity=53.2133676092545, Blast_Score=418, Evalue=1e-118,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 42913; Mature: 42781

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLDAYFGEYGGQYVPQILVPALEQLEQAFIDAQEDPDFRSEFMTLLQEYAGRPTALTL
CCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHH
CRNLTKGTKTKLYLKREDLLHGGAHKTNQVLGQALLAKRMGKDEIIAETGAGQHGVATAL
HHHHCCCCCEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHCCCHHEEEECCCCCCHHHHHH
ACALLGLKCRVYMGAKDVERQSPNVFRMKLMGAEVIPVHSGSATLKDACNEALRDWSATY
HHHHHHHHEEEEECCHHHHHCCCCEEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHH
ETAHYLLGTAAGPHPFPTIVREFQRMIGEETKMQILAREGRLPDAVIACVGGGSNAIGMF
HHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEEEECCCCCHHHHH
ADFIEEESVKLIGVEPAGKGIDTDQHGAPLKHGKTGIFFGMKAPLMQDEHGQIEESYSVS
HHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHCCCCC
AGLDFPSVGPQHAHLNAIGRAEYGAVTDDEALDAFKILARNEGIIPALESSHALAYALQL
CCCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
AQAEPEKEQLLVVNLSGRGDKDIFTVHDILKEKGEI
HHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
AKLDAYFGEYGGQYVPQILVPALEQLEQAFIDAQEDPDFRSEFMTLLQEYAGRPTALTL
CCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHH
CRNLTKGTKTKLYLKREDLLHGGAHKTNQVLGQALLAKRMGKDEIIAETGAGQHGVATAL
HHHHCCCCCEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHCCCHHEEEECCCCCCHHHHHH
ACALLGLKCRVYMGAKDVERQSPNVFRMKLMGAEVIPVHSGSATLKDACNEALRDWSATY
HHHHHHHHEEEEECCHHHHHCCCCEEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHH
ETAHYLLGTAAGPHPFPTIVREFQRMIGEETKMQILAREGRLPDAVIACVGGGSNAIGMF
HHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEEEECCCCCHHHHH
ADFIEEESVKLIGVEPAGKGIDTDQHGAPLKHGKTGIFFGMKAPLMQDEHGQIEESYSVS
HHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHCCCCC
AGLDFPSVGPQHAHLNAIGRAEYGAVTDDEALDAFKILARNEGIIPALESSHALAYALQL
CCCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
AQAEPEKEQLLVVNLSGRGDKDIFTVHDILKEKGEI
HHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1773058; 12620739 [H]