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The map label for this gene is trpA

Identifier: 59711634

GI number: 59711634

Start: 1131685

End: 1132491

Strand: Reverse

Name: trpA

Synonym: VF_1027

Alternate gene names: 59711634

Gene position: 1132491-1131685 (Counterclockwise)

Preceding gene: 59711635

Following gene: 59711633

Centisome position: 39.08

GC content: 42.63

Gene sequence:

>807_bases
ATGGATCGTTATCAAGCCCTATTTGCTCAGCTAGAGAAAAAAAATCAAGGAGCCTTTGTTCCATTCGTAACTATTGGCGA
TCCAAACCCTGAGCTATCTTATGACATCATGGAAACCCTGATTGAAGCTGGTGCCGATGCATTAGAATTAGGTATTCCGT
TTTCGGATCCTCTTGCTGATGGCCCTACTATTCAAGGCGCAAACATTCGAGCATTGGATAGCAAAACAACACCAGCAATT
TGTTTTGAGCTCATTACAAAAATTCGCTCTAAATATCCTGATACGCCAATCGGTCTTTTAGTTTATGCGAACCTTGTTTT
CGCAAATGGTATTGATAACTTCTACACAAAATGCCAACAAGCAGGTGTAGACTCTGTATTGATCGCCGATGTACCGACTA
ATGAATCCCAAGAATTCAGAGAAAGTGCAATAGAACATGGTATTCACCCTATTTTCATTGCACCACCATCAGCATCGCCT
GAAACACTAGAAACAGTTGCTAAATTAGGTGGCGGTTACACTTACCTTCTTTCTCGTGCAGGTGTTACTGGTGCCGAAAC
CAAAGCGGGTATGCCTGTTGCTCAATTACTAGAGCGCCTTAATCAATACGATGCACCACCGGCTATTCTTGGTTTTGGTA
TCTCAGAGCCAGCTCAAGTAGAAGAAGCGGTAAAAGCTGGAGCTGCAGGTGCTATCTCAGGCTCTGCAACGGTTAAATTG
ATTGAACAACATCAAGCTAACCCAGAGGCATTATTAAAAGCACTTGCGGACTTCACATCGTCAATGAAAGCAGCAACACA
AAAATAA

Upstream 100 bases:

>100_bases
AACCGGAGAAAGAACAACTATTGGTAGTAAACTTATCTGGCCGAGGCGATAAAGACATCTTCACAGTACATGACATTTTA
AAAGAAAAGGGAGAAATCTA

Downstream 100 bases:

>100_bases
TACATCTAATTAATCATGATTAAAGAGGCTAAATTAAGCCTCTTTTTTTTCCTCGATATATACCAACCTAATTACCCTCT
CCTAAATTGTTAATAAAATA

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MDRYQALFAQLEKKNQGAFVPFVTIGDPNPELSYDIMETLIEAGADALELGIPFSDPLADGPTIQGANIRALDSKTTPAI
CFELITKIRSKYPDTPIGLLVYANLVFANGIDNFYTKCQQAGVDSVLIADVPTNESQEFRESAIEHGIHPIFIAPPSASP
ETLETVAKLGGGYTYLLSRAGVTGAETKAGMPVAQLLERLNQYDAPPAILGFGISEPAQVEEAVKAGAAGAISGSATVKL
IEQHQANPEALLKALADFTSSMKAATQK

Sequences:

>Translated_268_residues
MDRYQALFAQLEKKNQGAFVPFVTIGDPNPELSYDIMETLIEAGADALELGIPFSDPLADGPTIQGANIRALDSKTTPAI
CFELITKIRSKYPDTPIGLLVYANLVFANGIDNFYTKCQQAGVDSVLIADVPTNESQEFRESAIEHGIHPIFIAPPSASP
ETLETVAKLGGGYTYLLSRAGVTGAETKAGMPVAQLLERLNQYDAPPAILGFGISEPAQVEEAVKAGAAGAISGSATVKL
IEQHQANPEALLKALADFTSSMKAATQK
>Mature_268_residues
MDRYQALFAQLEKKNQGAFVPFVTIGDPNPELSYDIMETLIEAGADALELGIPFSDPLADGPTIQGANIRALDSKTTPAI
CFELITKIRSKYPDTPIGLLVYANLVFANGIDNFYTKCQQAGVDSVLIADVPTNESQEFRESAIEHGIHPIFIAPPSASP
ETLETVAKLGGGYTYLLSRAGVTGAETKAGMPVAQLLERLNQYDAPPAILGFGISEPAQVEEAVKAGAAGAISGSATVKL
IEQHQANPEALLKALADFTSSMKAATQK

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=267, Percent_Identity=61.4232209737828, Blast_Score=325, Evalue=2e-90,
Organism=Saccharomyces cerevisiae, GI6321412, Length=241, Percent_Identity=32.3651452282158, Blast_Score=134, Evalue=2e-32,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_VIBF1 (Q5E624)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_204410.1
- ProteinModelPortal:   Q5E624
- SMR:   Q5E624
- STRING:   Q5E624
- GeneID:   3277533
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_1027
- NMPDR:   fig|312309.3.peg.880
- eggNOG:   COG0159
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- PhylomeDB:   Q5E624
- ProtClustDB:   PRK13111
- BioCyc:   VFIS312309:VF1027-MONOMER
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 28410; Mature: 28410

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 49-49 ACT_SITE 60-60

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRYQALFAQLEKKNQGAFVPFVTIGDPNPELSYDIMETLIEAGADALELGIPFSDPLAD
CCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCC
GPTIQGANIRALDSKTTPAICFELITKIRSKYPDTPIGLLVYANLVFANGIDNFYTKCQQ
CCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHH
AGVDSVLIADVPTNESQEFRESAIEHGIHPIFIAPPSASPETLETVAKLGGGYTYLLSRA
CCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEHHHC
GVTGAETKAGMPVAQLLERLNQYDAPPAILGFGISEPAQVEEAVKAGAAGAISGSATVKL
CCCCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHCCCCCCCCCHHHHHH
IEQHQANPEALLKALADFTSSMKAATQK
HHHHCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDRYQALFAQLEKKNQGAFVPFVTIGDPNPELSYDIMETLIEAGADALELGIPFSDPLAD
CCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCC
GPTIQGANIRALDSKTTPAICFELITKIRSKYPDTPIGLLVYANLVFANGIDNFYTKCQQ
CCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHH
AGVDSVLIADVPTNESQEFRESAIEHGIHPIFIAPPSASPETLETVAKLGGGYTYLLSRA
CCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEHHHC
GVTGAETKAGMPVAQLLERLNQYDAPPAILGFGISEPAQVEEAVKAGAAGAISGSATVKL
CCCCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHCCCCCCCCCHHHHHH
IEQHQANPEALLKALADFTSSMKAATQK
HHHHCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA