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The map label for this gene is rsuA [H]

Identifier: 59711487

GI number: 59711487

Start: 965702

End: 966406

Strand: Direct

Name: rsuA [H]

Synonym: VF_0880

Alternate gene names: 59711487

Gene position: 965702-966406 (Clockwise)

Preceding gene: 59711486

Following gene: 59711488

Centisome position: 33.33

GC content: 39.86

Gene sequence:

>705_bases
ATGCGTTTAGATAAATTTTTGTGCGAAACATTAGGTGCCACTCGTAAAGAAGCAACTCGAATCATTAAAAGTGGTACGGT
TACTGTTGATGGCGTGAAAACAAAAGTAACATCTGTAAAAGTAACAGATGATTCAACAGTTGAATGGGATGGACGTGAAC
TGAAATTCCATGGCCCTCGCTACATTATGCTATTTAAACCAGATGGTTATGTATGTTCTCATGAAGACAGTACGCACCCA
TCTGCACTCTCTTTATTAGATGAAGTGAATCTTGAGAAGCTGCATTTTGCAGGCCGTCTTGATGTAGACACTACCGGTTT
AGTACTTATTACGGATGACGGTCAATGGTCACATAAGATCACATCACCAAAACGTAAGTGTGCGAAAACATACCGTGTAT
GGCTTGCTGATCCAATTGGTGCTGATTACGCTGAGAAATTGGAAGCGGGTATTCAGCTTAAGAGCGAAAAAGATCTAACG
ATGCCAGCAGTAATGGAAATTGTTGATGAAGAAGAACACGAACTTCTATTAACTATCCATGAAGGTAAATACCACCAAGT
TAAGCGTATGTTTGCAGCTTTAGGTAATAAAGTTGAAGGTTTACACCGTGCTCAAATCGGTGGTTTAGGTTTGGATTATT
CTCTAGAACCAGGTGAGTACCGTTACTTAACTGAAGAAGAAGTTGAAGCGGTATTTGGCGAATAA

Upstream 100 bases:

>100_bases
TGATCCTATGGCAGGCGGCGCACACTAACAAATCTGGGTTGATGCTCGCATCTTTAAAAGGTTTGGGTATAATGCGCCAG
AATATTGAAATGAGGTTCTC

Downstream 100 bases:

>100_bases
TTAATCAGCCTTATCAATCCAATATTAAAAACGGCGTGGCATTTATGTCTCGCCGTTTTTTTGTGTAAATTTTATGCAAA
GTGCTCGAATATAAAAGGCA

Product: 16S rRNA pseudouridylate 516 synthase

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A [H]

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MRLDKFLCETLGATRKEATRIIKSGTVTVDGVKTKVTSVKVTDDSTVEWDGRELKFHGPRYIMLFKPDGYVCSHEDSTHP
SALSLLDEVNLEKLHFAGRLDVDTTGLVLITDDGQWSHKITSPKRKCAKTYRVWLADPIGADYAEKLEAGIQLKSEKDLT
MPAVMEIVDEEEHELLLTIHEGKYHQVKRMFAALGNKVEGLHRAQIGGLGLDYSLEPGEYRYLTEEEVEAVFGE

Sequences:

>Translated_234_residues
MRLDKFLCETLGATRKEATRIIKSGTVTVDGVKTKVTSVKVTDDSTVEWDGRELKFHGPRYIMLFKPDGYVCSHEDSTHP
SALSLLDEVNLEKLHFAGRLDVDTTGLVLITDDGQWSHKITSPKRKCAKTYRVWLADPIGADYAEKLEAGIQLKSEKDLT
MPAVMEIVDEEEHELLLTIHEGKYHQVKRMFAALGNKVEGLHRAQIGGLGLDYSLEPGEYRYLTEEEVEAVFGE
>Mature_234_residues
MRLDKFLCETLGATRKEATRIIKSGTVTVDGVKTKVTSVKVTDDSTVEWDGRELKFHGPRYIMLFKPDGYVCSHEDSTHP
SALSLLDEVNLEKLHFAGRLDVDTTGLVLITDDGQWSHKITSPKRKCAKTYRVWLADPIGADYAEKLEAGIQLKSEKDLT
MPAVMEIVDEEEHELLLTIHEGKYHQVKRMFAALGNKVEGLHRAQIGGLGLDYSLEPGEYRYLTEEEVEAVFGE

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=232, Percent_Identity=56.0344827586207, Blast_Score=257, Evalue=5e-70,
Organism=Escherichia coli, GI1787524, Length=231, Percent_Identity=28.1385281385281, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1790453, Length=239, Percent_Identity=28.0334728033473, Blast_Score=78, Evalue=4e-16,
Organism=Escherichia coli, GI87081838, Length=183, Percent_Identity=28.9617486338798, Blast_Score=67, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70

Molecular weight: Translated: 26309; Mature: 26309

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDKFLCETLGATRKEATRIIKSGTVTVDGVKTKVTSVKVTDDSTVEWDGRELKFHGPR
CCHHHHHHHHHCCCHHHHHHHHHCCCEEECCCEEEEEEEEECCCCEEEECCCEEEEECCE
YIMLFKPDGYVCSHEDSTHPSALSLLDEVNLEKLHFAGRLDVDTTGLVLITDDGQWSHKI
EEEEECCCCEEECCCCCCCHHHHHHHHHCCHHHEEECCCCCCCCCCEEEEECCCCCCCCC
TSPKRKCAKTYRVWLADPIGADYAEKLEAGIQLKSEKDLTMPAVMEIVDEEEHELLLTIH
CCHHHHHHHHHEEEEECCCCCHHHHHHHHCEEEECCCCCCHHHHHHHHCCCCCEEEEEEE
EGKYHQVKRMFAALGNKVEGLHRAQIGGLGLDYSLEPGEYRYLTEEEVEAVFGE
CCCHHHHHHHHHHHCCHHCCHHHHHHCCCCCCCCCCCCCEEEECHHHHHHHCCC
>Mature Secondary Structure
MRLDKFLCETLGATRKEATRIIKSGTVTVDGVKTKVTSVKVTDDSTVEWDGRELKFHGPR
CCHHHHHHHHHCCCHHHHHHHHHCCCEEECCCEEEEEEEEECCCCEEEECCCEEEEECCE
YIMLFKPDGYVCSHEDSTHPSALSLLDEVNLEKLHFAGRLDVDTTGLVLITDDGQWSHKI
EEEEECCCCEEECCCCCCCHHHHHHHHHCCHHHEEECCCCCCCCCCEEEEECCCCCCCCC
TSPKRKCAKTYRVWLADPIGADYAEKLEAGIQLKSEKDLTMPAVMEIVDEEEHELLLTIH
CCHHHHHHHHHEEEEECCCCCHHHHHHHHCEEEECCCCCCHHHHHHHHCCCCCEEEEEEE
EGKYHQVKRMFAALGNKVEGLHRAQIGGLGLDYSLEPGEYRYLTEEEVEAVFGE
CCCHHHHHHHHHHHCCHHCCHHHHHHCCCCCCCCCCCCCEEEECHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA