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The map label for this gene is emrB [H]

Identifier: 59711486

GI number: 59711486

Start: 964028

End: 965629

Strand: Direct

Name: emrB [H]

Synonym: VF_0879

Alternate gene names: 59711486

Gene position: 964028-965629 (Clockwise)

Preceding gene: 59711485

Following gene: 59711487

Centisome position: 33.27

GC content: 42.2

Gene sequence:

>1602_bases
ATGAGTCAATCGGTAAGTATTCCTGAAGGTGAGGTGGTTGATAAACCACTTGAACCTGAGATCCCACGTCGTCATTGGAT
AGCATTATTTGGTGGTTTGATTGGTGCCTTTATGGCGATCCTTGATATTCAAATCACCAACTCATCTCTAAAAGACATTC
AAGGTGCATTATCGGCAACCATGGATGAGAGTTCTTGGATTTCCACCTCTTATCTTGTTGCTGAAATGATAGCGATCCCA
TTAAGTGGTTGGCTGTCTGTGGCGCTTGGTAAACGTCGTTATCTAACATGGACAACCGTCGTATTTACCGTTGCTTCATT
ACTGTGTTCTTTTTCATGGAACATGGGCTCGATGATTGTGTTTCGTGCCATTCAAGGCTTCAGTGGTGGTGCGTTAATTC
CTCTGGCGTTTTCTCTTGTGGTTCACTTATTACCTGTGAACAAACGTGCGGTGGGGATGGCTCTGTTTGGGGTAACGGCG
ACCTTTGCTCCTTCTATTGGTCCGACGCTTGGTGGTTGGTTAACTGAGCATTTTTCTTGGCACTATATTTTTTATATCAA
CCTGCCACCGGCAATCGCTTTACTATGTATGGTGAATTACGGTCTGGATGATGAGCCATTAAAGCTAGAAAGCCTATTGA
AGGCCGATTGGCTTGGTATTATCACGATGGCGATAGGTTTGGGTTGTTTAGAAGTGGTACTTGAAGAAGGGAACCGTGAA
GAATGGTTTAGCTCAAGCTTTATCATTACGTTATCTATTATCTCAGTAATTAGCTTAACCTATTTTATTATCAATGAGTT
GCAGCATAAAAAACCATTGGTGAACTTGCGGTTATTAAAACAAAGTCAGTTTGCCATGTCGTGTATTGCGTATTTGATTT
TGGGTATGGCGCTGCTGGGTTCTATCTATGTGTTGCCAATGTATATGATCCAAATTCAAGGATATAACGCCTTGGAGATT
GGTGAAGTACTAATGTGGATGGGTTTTCCTCAATTATTGATCTTCCCATTAGTGCCCAAATTAACGCAGATCATTAAAGC
AAAATATTTGGTGTTCTTTGGTTTCTCTATGTTTGGTATTAGCTGCTTTGTAAATACACACATGAGTTATTTATTTGGTG
GTGATCAGCTTATTTTCTCCATGATATTACGTGCGATTGGCAGTCCGTTTATTATGGTTCCTTTGTCGTTGGTGGCAATG
GAAGGGATCCAAAAGCAAGAAGTGGCCGATGCATCAACCATTACTAATGTTCTGAGAAACCTTGGTGGTGCGTTCAGTAT
TGCGTTTATTGCGACGCTATTGGACAACAAAACACGTGAGCATTTGGCGCACATTAAAGAAACGTTAACCAGTGTAAGTA
CGGATGGTTGGTATCAATTAAACCAGAGTGCTGCGATGTTTGTAGCCAAAGGCAGCGATCCGACAACGGCAATGCAGCAA
GCCAAGGCATCATTGACGTTAACTATGCAGCGAGATGCGGCGATCATGGCGTATAATGATGTCTTTTTTATGATGTCCTG
CTTCCTTGCTTTTGCTGCGGTTTTGATGTTTTTTGTTAAATCGAATGGCAAGCCTGATCCTATGGCAGGCGGCGCACACT
AA

Upstream 100 bases:

>100_bases
GTGTGCCGGTTAAGATCAGCATTTTAAATACTGATTTACTGGACGGTAAATTGATCCCGGGTCTGTCTGTTGTTGCGACT
GTTGATACCCGAGGTTAAGC

Downstream 100 bases:

>100_bases
CAAATCTGGGTTGATGCTCGCATCTTTAAAAGGTTTGGGTATAATGCGCCAGAATATTGAAATGAGGTTCTCATGCGTTT
AGATAAATTTTTGTGCGAAA

Product: multidrug efflux system protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 533; Mature: 532

Protein sequence:

>533_residues
MSQSVSIPEGEVVDKPLEPEIPRRHWIALFGGLIGAFMAILDIQITNSSLKDIQGALSATMDESSWISTSYLVAEMIAIP
LSGWLSVALGKRRYLTWTTVVFTVASLLCSFSWNMGSMIVFRAIQGFSGGALIPLAFSLVVHLLPVNKRAVGMALFGVTA
TFAPSIGPTLGGWLTEHFSWHYIFYINLPPAIALLCMVNYGLDDEPLKLESLLKADWLGIITMAIGLGCLEVVLEEGNRE
EWFSSSFIITLSIISVISLTYFIINELQHKKPLVNLRLLKQSQFAMSCIAYLILGMALLGSIYVLPMYMIQIQGYNALEI
GEVLMWMGFPQLLIFPLVPKLTQIIKAKYLVFFGFSMFGISCFVNTHMSYLFGGDQLIFSMILRAIGSPFIMVPLSLVAM
EGIQKQEVADASTITNVLRNLGGAFSIAFIATLLDNKTREHLAHIKETLTSVSTDGWYQLNQSAAMFVAKGSDPTTAMQQ
AKASLTLTMQRDAAIMAYNDVFFMMSCFLAFAAVLMFFVKSNGKPDPMAGGAH

Sequences:

>Translated_533_residues
MSQSVSIPEGEVVDKPLEPEIPRRHWIALFGGLIGAFMAILDIQITNSSLKDIQGALSATMDESSWISTSYLVAEMIAIP
LSGWLSVALGKRRYLTWTTVVFTVASLLCSFSWNMGSMIVFRAIQGFSGGALIPLAFSLVVHLLPVNKRAVGMALFGVTA
TFAPSIGPTLGGWLTEHFSWHYIFYINLPPAIALLCMVNYGLDDEPLKLESLLKADWLGIITMAIGLGCLEVVLEEGNRE
EWFSSSFIITLSIISVISLTYFIINELQHKKPLVNLRLLKQSQFAMSCIAYLILGMALLGSIYVLPMYMIQIQGYNALEI
GEVLMWMGFPQLLIFPLVPKLTQIIKAKYLVFFGFSMFGISCFVNTHMSYLFGGDQLIFSMILRAIGSPFIMVPLSLVAM
EGIQKQEVADASTITNVLRNLGGAFSIAFIATLLDNKTREHLAHIKETLTSVSTDGWYQLNQSAAMFVAKGSDPTTAMQQ
AKASLTLTMQRDAAIMAYNDVFFMMSCFLAFAAVLMFFVKSNGKPDPMAGGAH
>Mature_532_residues
SQSVSIPEGEVVDKPLEPEIPRRHWIALFGGLIGAFMAILDIQITNSSLKDIQGALSATMDESSWISTSYLVAEMIAIPL
SGWLSVALGKRRYLTWTTVVFTVASLLCSFSWNMGSMIVFRAIQGFSGGALIPLAFSLVVHLLPVNKRAVGMALFGVTAT
FAPSIGPTLGGWLTEHFSWHYIFYINLPPAIALLCMVNYGLDDEPLKLESLLKADWLGIITMAIGLGCLEVVLEEGNREE
WFSSSFIITLSIISVISLTYFIINELQHKKPLVNLRLLKQSQFAMSCIAYLILGMALLGSIYVLPMYMIQIQGYNALEIG
EVLMWMGFPQLLIFPLVPKLTQIIKAKYLVFFGFSMFGISCFVNTHMSYLFGGDQLIFSMILRAIGSPFIMVPLSLVAME
GIQKQEVADASTITNVLRNLGGAFSIAFIATLLDNKTREHLAHIKETLTSVSTDGWYQLNQSAAMFVAKGSDPTTAMQQA
KASLTLTMQRDAAIMAYNDVFFMMSCFLAFAAVLMFFVKSNGKPDPMAGGAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=489, Percent_Identity=28.0163599182004, Blast_Score=239, Evalue=3e-64,
Organism=Escherichia coli, GI1788710, Length=489, Percent_Identity=29.6523517382413, Blast_Score=211, Evalue=7e-56,
Organism=Escherichia coli, GI1790195, Length=289, Percent_Identity=28.0276816608997, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI87081983, Length=427, Percent_Identity=25.0585480093677, Blast_Score=99, Evalue=9e-22,
Organism=Escherichia coli, GI1788392, Length=274, Percent_Identity=28.1021897810219, Blast_Score=93, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6325455, Length=159, Percent_Identity=29.559748427673, Blast_Score=81, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6323735, Length=327, Percent_Identity=23.5474006116208, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319770, Length=278, Percent_Identity=26.6187050359712, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6321663, Length=148, Percent_Identity=25, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 58638; Mature: 58507

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSVSIPEGEVVDKPLEPEIPRRHWIALFGGLIGAFMAILDIQITNSSLKDIQGALSAT
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHH
MDESSWISTSYLVAEMIAIPLSGWLSVALGKRRYLTWTTVVFTVASLLCSFSWNMGSMIV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHH
FRAIQGFSGGALIPLAFSLVVHLLPVNKRAVGMALFGVTATFAPSIGPTLGGWLTEHFSW
HHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCE
HYIFYINLPPAIALLCMVNYGLDDEPLKLESLLKADWLGIITMAIGLGCLEVVLEEGNRE
EEEEEEECCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
EWFSSSFIITLSIISVISLTYFIINELQHKKPLVNLRLLKQSQFAMSCIAYLILGMALLG
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SIYVLPMYMIQIQGYNALEIGEVLMWMGFPQLLIFPLVPKLTQIIKAKYLVFFGFSMFGI
HHHHHHHHHHEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SCFVNTHMSYLFGGDQLIFSMILRAIGSPFIMVPLSLVAMEGIQKQEVADASTITNVLRN
HHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
LGGAFSIAFIATLLDNKTREHLAHIKETLTSVSTDGWYQLNQSAAMFVAKGSDPTTAMQQ
CCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEECCCEEEEEECCCCCHHHHHH
AKASLTLTMQRDAAIMAYNDVFFMMSCFLAFAAVLMFFVKSNGKPDPMAGGAH
HHHHEEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SQSVSIPEGEVVDKPLEPEIPRRHWIALFGGLIGAFMAILDIQITNSSLKDIQGALSAT
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHH
MDESSWISTSYLVAEMIAIPLSGWLSVALGKRRYLTWTTVVFTVASLLCSFSWNMGSMIV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHH
FRAIQGFSGGALIPLAFSLVVHLLPVNKRAVGMALFGVTATFAPSIGPTLGGWLTEHFSW
HHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCE
HYIFYINLPPAIALLCMVNYGLDDEPLKLESLLKADWLGIITMAIGLGCLEVVLEEGNRE
EEEEEEECCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
EWFSSSFIITLSIISVISLTYFIINELQHKKPLVNLRLLKQSQFAMSCIAYLILGMALLG
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SIYVLPMYMIQIQGYNALEIGEVLMWMGFPQLLIFPLVPKLTQIIKAKYLVFFGFSMFGI
HHHHHHHHHHEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SCFVNTHMSYLFGGDQLIFSMILRAIGSPFIMVPLSLVAMEGIQKQEVADASTITNVLRN
HHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
LGGAFSIAFIATLLDNKTREHLAHIKETLTSVSTDGWYQLNQSAAMFVAKGSDPTTAMQQ
CCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEECCCEEEEEECCCCCHHHHHH
AKASLTLTMQRDAAIMAYNDVFFMMSCFLAFAAVLMFFVKSNGKPDPMAGGAH
HHHHEEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]