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The map label for this gene is bcr [H]

Identifier: 59711488

GI number: 59711488

Start: 966621

End: 967826

Strand: Direct

Name: bcr [H]

Synonym: VF_0881

Alternate gene names: 59711488

Gene position: 966621-967826 (Clockwise)

Preceding gene: 59711487

Following gene: 59711489

Centisome position: 33.36

GC content: 41.63

Gene sequence:

>1206_bases
ATGTCATCCACTGAAACCGCTCTTTCTGAGCCCCCAAAATTAAGCATACTGCTGGTTATTATTTTAGGTGCAATTTCTGC
ACTCACTCCATTAGCTATTGATATGTATGTACCTGCGATGCCGAGTATTGCTTCTGACTTAGGAGTAACTGCTGGTTCAG
TACAAATGACATTAACCGCTTATACTGCGGGCTTTGCCATTGCCCAATTAATTCATGGCCCGTTAGCTGATAGTTATGGT
CGACGTCCAGTCTTGATTATTGGTACGGTCTTATTTGCTATTTGTGCGGTCATTGGTGCTTTAGTTGGTGGCATTGAATC
GTTGATGTACATTCGTGTTTTACAAGGTGTTGCTGGTGCAGCTTCTGCGGTAGTGGTTCAAGCTATTGTTCGTGACATGT
TTAATAAAGAAGATTTCGCACGAATGATGGCATTCATTACTTTGGTGATGACAGTAGCACCTCTGGTTGCCCCTATGGTG
GGTGGACACATGGCGGTGTGGTTTGGCTGGCGTTCTATTTTTTGGCTGTTAGCGGGATTCGCTGTACTGATTATTTTGGC
TATTTTATGGCGTATTCCTGAAACCTTAGCAGAAGAGAACCGTTCACCGTTTAGGCTAATGACGTCACTACGAAATTACC
GCTCGTTATTTAAAAATCCTGTTTCTCTTGGTTTGATTTTTTCTGGTGCGTTTTCTTTCGCTGGTATGTTTTCATTTTTA
ACCGCTGGTTCGTTTGTTTACATTGATATTTATGGTGTGAGTGTTTCGAACTTTGGTTATCTGTTTGGCCTTAATGTGGT
GTTCTTAATTATTATGACCACGATCAATGGCCGTATTGTTAAGAAAATGGGTTCTCACAATATGGTGAAATTGGGTATCT
CAATCCAATTATTCGCGGGTATTTTGATGGTTGTAGGCCAATTACTCGATTGGGGTTTATGGGGTACTGTGGTTCCAGTG
GTTTTATTTGTTGGCTGTATTTCTACCATAGGTAGTAACACCATGGGCTTATTATTAAGTCATTACCCTAAAATGGCAGG
TACTGCTTCGTCATTAGCGGGTACACTACGTTTTGGTACTGGCTCATTGGTTGGGGCGTGTATTGCAATGTTCCCTTCTG
ATTCGGCTTGGCCTATGGTAAGTTCCATGGCAGCATGTTCTATATTATCTTTTGGTTGTTATTTTGTGTTTGGACGAAAA
GCGTAA

Upstream 100 bases:

>100_bases
ATGATACTATGATAAATCGAATAAGTATCTGATCATTCTTGCTTGAATCGGTATTGTTTGAACTCCTGTCCTGCTGTTTT
ATTTTATTGGTATCGCCGTT

Downstream 100 bases:

>100_bases
ATGAGTAAAGAAAAAAAAGCAGTTGATTATTATCAAGAAATTCAACGAGTAATTAATAGTGCTCTTGGTGATCTACAAGA
GTCTAAAGACAGTGGCCGTT

Product: bicyclomycin/multidrug efflux system

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MSSTETALSEPPKLSILLVIILGAISALTPLAIDMYVPAMPSIASDLGVTAGSVQMTLTAYTAGFAIAQLIHGPLADSYG
RRPVLIIGTVLFAICAVIGALVGGIESLMYIRVLQGVAGAASAVVVQAIVRDMFNKEDFARMMAFITLVMTVAPLVAPMV
GGHMAVWFGWRSIFWLLAGFAVLIILAILWRIPETLAEENRSPFRLMTSLRNYRSLFKNPVSLGLIFSGAFSFAGMFSFL
TAGSFVYIDIYGVSVSNFGYLFGLNVVFLIIMTTINGRIVKKMGSHNMVKLGISIQLFAGILMVVGQLLDWGLWGTVVPV
VLFVGCISTIGSNTMGLLLSHYPKMAGTASSLAGTLRFGTGSLVGACIAMFPSDSAWPMVSSMAACSILSFGCYFVFGRK
A

Sequences:

>Translated_401_residues
MSSTETALSEPPKLSILLVIILGAISALTPLAIDMYVPAMPSIASDLGVTAGSVQMTLTAYTAGFAIAQLIHGPLADSYG
RRPVLIIGTVLFAICAVIGALVGGIESLMYIRVLQGVAGAASAVVVQAIVRDMFNKEDFARMMAFITLVMTVAPLVAPMV
GGHMAVWFGWRSIFWLLAGFAVLIILAILWRIPETLAEENRSPFRLMTSLRNYRSLFKNPVSLGLIFSGAFSFAGMFSFL
TAGSFVYIDIYGVSVSNFGYLFGLNVVFLIIMTTINGRIVKKMGSHNMVKLGISIQLFAGILMVVGQLLDWGLWGTVVPV
VLFVGCISTIGSNTMGLLLSHYPKMAGTASSLAGTLRFGTGSLVGACIAMFPSDSAWPMVSSMAACSILSFGCYFVFGRK
A
>Mature_400_residues
SSTETALSEPPKLSILLVIILGAISALTPLAIDMYVPAMPSIASDLGVTAGSVQMTLTAYTAGFAIAQLIHGPLADSYGR
RPVLIIGTVLFAICAVIGALVGGIESLMYIRVLQGVAGAASAVVVQAIVRDMFNKEDFARMMAFITLVMTVAPLVAPMVG
GHMAVWFGWRSIFWLLAGFAVLIILAILWRIPETLAEENRSPFRLMTSLRNYRSLFKNPVSLGLIFSGAFSFAGMFSFLT
AGSFVYIDIYGVSVSNFGYLFGLNVVFLIIMTTINGRIVKKMGSHNMVKLGISIQLFAGILMVVGQLLDWGLWGTVVPVV
LFVGCISTIGSNTMGLLLSHYPKMAGTASSLAGTLRFGTGSLVGACIAMFPSDSAWPMVSSMAACSILSFGCYFVFGRKA

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=373, Percent_Identity=50.1340482573727, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI48994889, Length=247, Percent_Identity=34.412955465587, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI87082312, Length=351, Percent_Identity=25.3561253561254, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI1790794, Length=288, Percent_Identity=25.6944444444444, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1790146, Length=242, Percent_Identity=27.6859504132231, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1787065, Length=186, Percent_Identity=29.5698924731183, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI2367379, Length=206, Percent_Identity=25.2427184466019, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1790195, Length=136, Percent_Identity=27.2058823529412, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319517, Length=199, Percent_Identity=25.6281407035176, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324264, Length=215, Percent_Identity=23.2558139534884, Blast_Score=67, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6319657, Length=232, Percent_Identity=25.4310344827586, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323000, Length=185, Percent_Identity=26.4864864864865, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6325414, Length=139, Percent_Identity=33.0935251798561, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319480, Length=289, Percent_Identity=23.5294117647059, Blast_Score=63, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42689; Mature: 42557

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTETALSEPPKLSILLVIILGAISALTPLAIDMYVPAMPSIASDLGVTAGSVQMTLTA
CCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCEEEHHH
YTAGFAIAQLIHGPLADSYGRRPVLIIGTVLFAICAVIGALVGGIESLMYIRVLQGVAGA
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
ASAVVVQAIVRDMFNKEDFARMMAFITLVMTVAPLVAPMVGGHMAVWFGWRSIFWLLAGF
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
AVLIILAILWRIPETLAEENRSPFRLMTSLRNYRSLFKNPVSLGLIFSGAFSFAGMFSFL
HHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
TAGSFVYIDIYGVSVSNFGYLFGLNVVFLIIMTTINGRIVKKMGSHNMVKLGISIQLFAG
HCCCEEEEEEEEECHHCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCEEEECHHHHHHHH
ILMVVGQLLDWGLWGTVVPVVLFVGCISTIGSNTMGLLLSHYPKMAGTASSLAGTLRFGT
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHCCCHHHHHHHHHCCC
GSLVGACIAMFPSDSAWPMVSSMAACSILSFGCYFVFGRKA
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHEECCCC
>Mature Secondary Structure 
SSTETALSEPPKLSILLVIILGAISALTPLAIDMYVPAMPSIASDLGVTAGSVQMTLTA
CCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCEEEHHH
YTAGFAIAQLIHGPLADSYGRRPVLIIGTVLFAICAVIGALVGGIESLMYIRVLQGVAGA
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
ASAVVVQAIVRDMFNKEDFARMMAFITLVMTVAPLVAPMVGGHMAVWFGWRSIFWLLAGF
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
AVLIILAILWRIPETLAEENRSPFRLMTSLRNYRSLFKNPVSLGLIFSGAFSFAGMFSFL
HHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
TAGSFVYIDIYGVSVSNFGYLFGLNVVFLIIMTTINGRIVKKMGSHNMVKLGISIQLFAG
HCCCEEEEEEEEECHHCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCEEEECHHHHHHHH
ILMVVGQLLDWGLWGTVVPVVLFVGCISTIGSNTMGLLLSHYPKMAGTASSLAGTLRFGT
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHCCCHHHHHHHHHCCC
GSLVGACIAMFPSDSAWPMVSSMAACSILSFGCYFVFGRKA
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]