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The map label for this gene is emrK [H]

Identifier: 59711485

GI number: 59711485

Start: 962985

End: 964025

Strand: Direct

Name: emrK [H]

Synonym: VF_0878

Alternate gene names: 59711485

Gene position: 962985-964025 (Clockwise)

Preceding gene: 59711480

Following gene: 59711486

Centisome position: 33.23

GC content: 39.77

Gene sequence:

>1041_bases
GTGACTGAATTAAAAAAGAAGAAAAATAAAGCACCGTTAATCGCAACAGTCATTTTTATTTTATGTGGCGCCTTGGGTGC
CGGTTATTGGTATGGCTACGGTCAGTATTTCCAATCAACGGATAATGCGTACCTTCAAGGCGATATCACAACAATTAGCT
CAAAAGTAGGTGGTTATATTCAACAAACCTTAGTTGAAGATAATCAGAAAGTAAAAGCAGGGGATTTATTAGCAACCATT
GATGATCGTGATTTCTTGGCTGAACAAGAAAAAGCGGAAGCTAATGTTGCTGTTAATGCGGCGGCAATTACGAATTTAAA
TGCTGAGCTTAAACTGCAAGATATCCGAATTCGTCAATCTGCAAGCCAAATTGAGTCGGCACAGGCGGATCTTGAGCGTG
CTCAACAACAAGTAAAACGTACCAAAGCGTTAATTAAAAAGAATTACTCTTCACAAGATGAATTGGATAATAACGTTTCA
CATTTGAAAGTGGCGATTGCGAATCTAGATGCAGCAAAAGCGAATTATCAAGCATCGAAAGAGCAAATCAACGTAATTGA
AAGCCAAATCGGTCAAGCAAATGCAATGCTAAACCAAGCAAAAACGGCGTTAGACCAAGCGAATTTGAATTTATCTTACA
CTAAGATTTACGCGCCAATTGATGGCATCGTTGGTAAACGCAGCTTACGTGAAGGTTTATTAGTTCAAGCAGGAATGCCA
CTACTTAGCTTGGTTCCAACCAAAGAAGTGTGGATTGAAGCGAACTTTAAAGAAACACAGTTAGAAAAAATTCATCATGG
TCAAAAAGTGTTTGTCGATCTTGATGCTTATCCTGATCTGCACCTAAAAGGGATTGTAGAAAGCTTCTCGCCAGCAACTG
GTGCTAAATTTGCACTTCTGCCACCAGAGAACGCAACAGGTAACTTTACGAAAATTGTGCAACGTGTGCCGGTTAAGATC
AGCATTTTAAATACTGATTTACTGGACGGTAAATTGATCCCGGGTCTGTCTGTTGTTGCGACTGTTGATACCCGAGGTTA
A

Upstream 100 bases:

>100_bases
CAACAGTGTTTCCTTTTTTTCCTATCTAATCAATTAATCTTTTTAGTTTTATACTCTGTATCCACAATATTAGAATTTTA
TAAAGATAGGTAAAGCCTCA

Downstream 100 bases:

>100_bases
GCATGAGTCAATCGGTAAGTATTCCTGAAGGTGAGGTGGTTGATAAACCACTTGAACCTGAGATCCCACGTCGTCATTGG
ATAGCATTATTTGGTGGTTT

Product: EmrKY-TolC multidrug resistance efflux pump, membrane fusion protein component

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MTELKKKKNKAPLIATVIFILCGALGAGYWYGYGQYFQSTDNAYLQGDITTISSKVGGYIQQTLVEDNQKVKAGDLLATI
DDRDFLAEQEKAEANVAVNAAAITNLNAELKLQDIRIRQSASQIESAQADLERAQQQVKRTKALIKKNYSSQDELDNNVS
HLKVAIANLDAAKANYQASKEQINVIESQIGQANAMLNQAKTALDQANLNLSYTKIYAPIDGIVGKRSLREGLLVQAGMP
LLSLVPTKEVWIEANFKETQLEKIHHGQKVFVDLDAYPDLHLKGIVESFSPATGAKFALLPPENATGNFTKIVQRVPVKI
SILNTDLLDGKLIPGLSVVATVDTRG

Sequences:

>Translated_346_residues
MTELKKKKNKAPLIATVIFILCGALGAGYWYGYGQYFQSTDNAYLQGDITTISSKVGGYIQQTLVEDNQKVKAGDLLATI
DDRDFLAEQEKAEANVAVNAAAITNLNAELKLQDIRIRQSASQIESAQADLERAQQQVKRTKALIKKNYSSQDELDNNVS
HLKVAIANLDAAKANYQASKEQINVIESQIGQANAMLNQAKTALDQANLNLSYTKIYAPIDGIVGKRSLREGLLVQAGMP
LLSLVPTKEVWIEANFKETQLEKIHHGQKVFVDLDAYPDLHLKGIVESFSPATGAKFALLPPENATGNFTKIVQRVPVKI
SILNTDLLDGKLIPGLSVVATVDTRG
>Mature_345_residues
TELKKKKNKAPLIATVIFILCGALGAGYWYGYGQYFQSTDNAYLQGDITTISSKVGGYIQQTLVEDNQKVKAGDLLATID
DRDFLAEQEKAEANVAVNAAAITNLNAELKLQDIRIRQSASQIESAQADLERAQQQVKRTKALIKKNYSSQDELDNNVSH
LKVAIANLDAAKANYQASKEQINVIESQIGQANAMLNQAKTALDQANLNLSYTKIYAPIDGIVGKRSLREGLLVQAGMPL
LSLVPTKEVWIEANFKETQLEKIHHGQKVFVDLDAYPDLHLKGIVESFSPATGAKFALLPPENATGNFTKIVQRVPVKIS
ILNTDLLDGKLIPGLSVVATVDTRG

Specific function: Unknown

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788711, Length=346, Percent_Identity=34.393063583815, Blast_Score=165, Evalue=4e-42,
Organism=Escherichia coli, GI1789041, Length=342, Percent_Identity=32.4561403508772, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1790519, Length=293, Percent_Identity=28.6689419795222, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI87081951, Length=327, Percent_Identity=26.9113149847095, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1789900, Length=296, Percent_Identity=25.6756756756757, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787013, Length=268, Percent_Identity=29.1044776119403, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 37780; Mature: 37648

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELKKKKNKAPLIATVIFILCGALGAGYWYGYGQYFQSTDNAYLQGDITTISSKVGGYI
CCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCEEEECHHHHHHHHHHHH
QQTLVEDNQKVKAGDLLATIDDRDFLAEQEKAEANVAVNAAAITNLNAELKLQDIRIRQS
HHHHHCCCCCEECCCEEEEECCHHHHHHHHHCCCCEEEEEEEEECCCCEEEEEHHHHHHH
ASQIESAQADLERAQQQVKRTKALIKKNYSSQDELDNNVSHLKVAIANLDAAKANYQASK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHEEEEEECCCHHHCCCHHHH
EQINVIESQIGQANAMLNQAKTALDQANLNLSYTKIYAPIDGIVGKRSLREGLLVQAGMP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECHHHHHHHHHHHCCCEECCCCC
LLSLVPTKEVWIEANFKETQLEKIHHGQKVFVDLDAYPDLHLKGIVESFSPATGAKFALL
HHHHCCCCCEEEECCCCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHCCCCCCCEEEEE
PPENATGNFTKIVQRVPVKISILNTDLLDGKLIPGLSVVATVDTRG
CCCCCCCHHHHHHHHCCEEEEEEECCCCCCCCCCCCEEEEEEECCC
>Mature Secondary Structure 
TELKKKKNKAPLIATVIFILCGALGAGYWYGYGQYFQSTDNAYLQGDITTISSKVGGYI
CCCHHHCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCEEEECHHHHHHHHHHHH
QQTLVEDNQKVKAGDLLATIDDRDFLAEQEKAEANVAVNAAAITNLNAELKLQDIRIRQS
HHHHHCCCCCEECCCEEEEECCHHHHHHHHHCCCCEEEEEEEEECCCCEEEEEHHHHHHH
ASQIESAQADLERAQQQVKRTKALIKKNYSSQDELDNNVSHLKVAIANLDAAKANYQASK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHEEEEEECCCHHHCCCHHHH
EQINVIESQIGQANAMLNQAKTALDQANLNLSYTKIYAPIDGIVGKRSLREGLLVQAGMP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECHHHHHHHHHHHCCCEECCCCC
LLSLVPTKEVWIEANFKETQLEKIHHGQKVFVDLDAYPDLHLKGIVESFSPATGAKFALL
HHHHCCCCCEEEECCCCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHCCCCCCCEEEEE
PPENATGNFTKIVQRVPVKISILNTDLLDGKLIPGLSVVATVDTRG
CCCCCCCHHHHHHHHCCEEEEEEECCCCCCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]