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The map label for this gene is guaA [H]

Identifier: 59711245

GI number: 59711245

Start: 695666

End: 697219

Strand: Direct

Name: guaA [H]

Synonym: VF_0638

Alternate gene names: 59711245

Gene position: 695666-697219 (Clockwise)

Preceding gene: 59711244

Following gene: 59711246

Centisome position: 24.01

GC content: 40.09

Gene sequence:

>1554_bases
ATGACGACTAATATCCACGATCAACGTATTCTTATTTTAGATTTTGGTTCTCAATATACGCAGCTTGTTGCGCGTCGTAT
TCGTGAAATCGGTGTTTACTGTGAGCTTTGGAGCTGGGATGTAGAAGAATCTGACATCCGTGATTTTAATCCAGACGGTA
TCATTTTATCTGGTGGCCCTGAGAGTGTTACAGAAGAAAACTCTCCTCGTGCACCACAATATGTATTTGATTCAGGTGTT
CCTGTATTTGGTGTGTGTTACGGCATGCAGACAATGGCAGAGCAACTGGGTGGTAAAGTAGCAACATCAACTGAGCGTGA
GTTTGGTTATGCAGCGGTACAAGTAACTGGTGAATCAGCGTTATTTAAAGATCTTGAAGCAACTCAAGATGTTTGGATGA
GTCACGGTGATAAAGTTGTTGAAATCCCATCTGATTTCGTAAAAATTGCAGAGACAGAAACCTGTCCATATGCTGCAATG
GCGAACGAAGAGAAGAAATACTACGGTGTTCAATTCCACCCAGAAGTAACTCACACTAAAAATGGCATGAAAATGCTTGA
GAACTTTGTTCTTAATGTGTGTGGTTGTGAAGGTTTATGGACATCAGCTTCTATTATCGAAGATGCCGTTGCTCGTATTA
AAGAGCAAGTGGGTGATGATGAAGTGATTCTTGGTTTATCTGGTGGCGTTGACTCATCAGTAGTTGCAATGCTTGCTCAC
CGTGCAATTGGTGACAAACTAACGTGTGTATTCGTTGATAACGGTTTATTACGTTTAAATGAAGCTGATCAGGTAATGGA
AATGTTTGGCAATAAATTTGGCCTAAACATTGTACATGTGAATGCTGAACAGCGTTTCCTTGATGCACTAGAAGGTGAAT
CAGATCCTGAAACAAAACGTAAGATCATTGGTCACGTATTCGTAGATATCTTTGATGAAGAATCGAAGAAACTGAAAAAT
GCTAAATGGTTAGCGCAAGGTACTATTTACCCTGATGTTATTGAATCAGCTGCATCTAAAACAGGTAAAGCTCACGTAAT
TAAATCACACCATAACGTTGGCGGCTTGCCAGATGATATGGAAATGGGTCTTGTTGAGCCTCTACGTGAATTATTTAAAG
ATGAAGTTCGTAAGATCGGTCTAGAACTAGGCTTACCTTATAACATGCTTTACCGCCACCCATTCCCGGGTCCAGGTTTA
GGTGTTCGTGTTCTTGGTGAAATTAAGAAAGAGTACTGTGATTTACTTCGCCGTGCAGATGCTATTTTCATTGAAGAATT
ACACAATGCAGATCTTTACAATAAAGTATCTCAAGCATTCACTGTATTCTTACCAGTTCGTTCTGTTGGTGTAATGGGTG
ACGGTCGTAAGTACGATTGGGTTGTTTCTCTACGTGCTGTTGAGACTATCGACTTTATGACTGCGCATTGGGCACATTTA
CCATACGATTTCTTAGGTAAAGTATCTAACCGTATTATTAATGAAGTTAATGGCATTTCTCGTGTAGTTTACGATATTTC
AGGTAAGCCACCAGCAACAATTGAGTGGGAATAA

Upstream 100 bases:

>100_bases
CTTTGAAATATAAAGAAAAAATACTTTGTACTAAATTCAATTTTAAGCGTGTGGTTTATATGAAAGCCACACTAAATAAA
TGATTTTTTGAGAAAGACTA

Downstream 100 bases:

>100_bases
TTCTTAATAATTGTTGATATGAATTTTGTTTATTAAAGCGCCGAAAGGCGCTTTTTTTGTTTCCATTGTAATGTAAATAT
TAACGTTGTTAGATAGATGT

Product: GMP synthase

Products: NA

Alternate protein names: GMP synthetase; Glutamine amidotransferase [H]

Number of amino acids: Translated: 517; Mature: 516

Protein sequence:

>517_residues
MTTNIHDQRILILDFGSQYTQLVARRIREIGVYCELWSWDVEESDIRDFNPDGIILSGGPESVTEENSPRAPQYVFDSGV
PVFGVCYGMQTMAEQLGGKVATSTEREFGYAAVQVTGESALFKDLEATQDVWMSHGDKVVEIPSDFVKIAETETCPYAAM
ANEEKKYYGVQFHPEVTHTKNGMKMLENFVLNVCGCEGLWTSASIIEDAVARIKEQVGDDEVILGLSGGVDSSVVAMLAH
RAIGDKLTCVFVDNGLLRLNEADQVMEMFGNKFGLNIVHVNAEQRFLDALEGESDPETKRKIIGHVFVDIFDEESKKLKN
AKWLAQGTIYPDVIESAASKTGKAHVIKSHHNVGGLPDDMEMGLVEPLRELFKDEVRKIGLELGLPYNMLYRHPFPGPGL
GVRVLGEIKKEYCDLLRRADAIFIEELHNADLYNKVSQAFTVFLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHL
PYDFLGKVSNRIINEVNGISRVVYDISGKPPATIEWE

Sequences:

>Translated_517_residues
MTTNIHDQRILILDFGSQYTQLVARRIREIGVYCELWSWDVEESDIRDFNPDGIILSGGPESVTEENSPRAPQYVFDSGV
PVFGVCYGMQTMAEQLGGKVATSTEREFGYAAVQVTGESALFKDLEATQDVWMSHGDKVVEIPSDFVKIAETETCPYAAM
ANEEKKYYGVQFHPEVTHTKNGMKMLENFVLNVCGCEGLWTSASIIEDAVARIKEQVGDDEVILGLSGGVDSSVVAMLAH
RAIGDKLTCVFVDNGLLRLNEADQVMEMFGNKFGLNIVHVNAEQRFLDALEGESDPETKRKIIGHVFVDIFDEESKKLKN
AKWLAQGTIYPDVIESAASKTGKAHVIKSHHNVGGLPDDMEMGLVEPLRELFKDEVRKIGLELGLPYNMLYRHPFPGPGL
GVRVLGEIKKEYCDLLRRADAIFIEELHNADLYNKVSQAFTVFLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHL
PYDFLGKVSNRIINEVNGISRVVYDISGKPPATIEWE
>Mature_516_residues
TTNIHDQRILILDFGSQYTQLVARRIREIGVYCELWSWDVEESDIRDFNPDGIILSGGPESVTEENSPRAPQYVFDSGVP
VFGVCYGMQTMAEQLGGKVATSTEREFGYAAVQVTGESALFKDLEATQDVWMSHGDKVVEIPSDFVKIAETETCPYAAMA
NEEKKYYGVQFHPEVTHTKNGMKMLENFVLNVCGCEGLWTSASIIEDAVARIKEQVGDDEVILGLSGGVDSSVVAMLAHR
AIGDKLTCVFVDNGLLRLNEADQVMEMFGNKFGLNIVHVNAEQRFLDALEGESDPETKRKIIGHVFVDIFDEESKKLKNA
KWLAQGTIYPDVIESAASKTGKAHVIKSHHNVGGLPDDMEMGLVEPLRELFKDEVRKIGLELGLPYNMLYRHPFPGPGLG
VRVLGEIKKEYCDLLRRADAIFIEELHNADLYNKVSQAFTVFLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLP
YDFLGKVSNRIINEVNGISRVVYDISGKPPATIEWE

Specific function: Catalyzes the synthesis of GMP from XMP [H]

COG id: COG0519

COG function: function code F; GMP synthase, PP-ATPase domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GMP-binding domain [H]

Homologues:

Organism=Homo sapiens, GI4504035, Length=554, Percent_Identity=35.9205776173285, Blast_Score=305, Evalue=6e-83,
Organism=Escherichia coli, GI1788854, Length=525, Percent_Identity=81.5238095238095, Blast_Score=903, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71992717, Length=577, Percent_Identity=34.8353552859619, Blast_Score=312, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI71992710, Length=577, Percent_Identity=34.8353552859619, Blast_Score=312, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI133901714, Length=577, Percent_Identity=34.8353552859619, Blast_Score=312, Evalue=2e-85,
Organism=Saccharomyces cerevisiae, GI6323873, Length=522, Percent_Identity=49.0421455938697, Blast_Score=527, Evalue=1e-150,
Organism=Saccharomyces cerevisiae, GI6322638, Length=235, Percent_Identity=28.5106382978723, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI281365319, Length=554, Percent_Identity=36.101083032491, Blast_Score=295, Evalue=7e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR001674
- InterPro:   IPR004739
- InterPro:   IPR022955
- InterPro:   IPR022310
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00117 GATase; PF00958 GMP_synt_C; PF02540 NAD_synthase [H]

EC number: =6.3.5.2 [H]

Molecular weight: Translated: 57708; Mature: 57577

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTNIHDQRILILDFGSQYTQLVARRIREIGVYCELWSWDVEESDIRDFNPDGIILSGGP
CCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCEEEECCCC
ESVTEENSPRAPQYVFDSGVPVFGVCYGMQTMAEQLGGKVATSTEREFGYAAVQVTGESA
CCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHCCCEECCCCHHCCEEEEEEECCHH
LFKDLEATQDVWMSHGDKVVEIPSDFVKIAETETCPYAAMANEEKKYYGVQFHPEVTHTK
HHHHHHHHHHHHHHCCCEEEECCHHHHHHHCCCCCCCHHHCCCCCCEECEEECCCCCCCH
NGMKMLENFVLNVCGCEGLWTSASIIEDAVARIKEQVGDDEVILGLSGGVDSSVVAMLAH
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHH
RAIGDKLTCVFVDNGLLRLNEADQVMEMFGNKFGLNIVHVNAEQRFLDALEGESDPETKR
HHCCCCEEEEEECCCEEEECCHHHHHHHHCCCCCCEEEEECHHHHHHHHHCCCCCHHHHH
KIIGHVFVDIFDEESKKLKNAKWLAQGTIYPDVIESAASKTGKAHVIKSHHNVGGLPDDM
HHHHHHHHHHHCHHHHHHHCCCEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCHH
EMGLVEPLRELFKDEVRKIGLELGLPYNMLYRHPFPGPGLGVRVLGEIKKEYCDLLRRAD
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
AIFIEELHNADLYNKVSQAFTVFLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHL
HHHHHHHHCCHHHHHHHHHHEEEEEHHHCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHC
PYDFLGKVSNRIINEVNGISRVVYDISGKPPATIEWE
CHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCEEECC
>Mature Secondary Structure 
TTNIHDQRILILDFGSQYTQLVARRIREIGVYCELWSWDVEESDIRDFNPDGIILSGGP
CCCCCCCEEEEEECCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCEEEECCCC
ESVTEENSPRAPQYVFDSGVPVFGVCYGMQTMAEQLGGKVATSTEREFGYAAVQVTGESA
CCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHCCCEECCCCHHCCEEEEEEECCHH
LFKDLEATQDVWMSHGDKVVEIPSDFVKIAETETCPYAAMANEEKKYYGVQFHPEVTHTK
HHHHHHHHHHHHHHCCCEEEECCHHHHHHHCCCCCCCHHHCCCCCCEECEEECCCCCCCH
NGMKMLENFVLNVCGCEGLWTSASIIEDAVARIKEQVGDDEVILGLSGGVDSSVVAMLAH
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHH
RAIGDKLTCVFVDNGLLRLNEADQVMEMFGNKFGLNIVHVNAEQRFLDALEGESDPETKR
HHCCCCEEEEEECCCEEEECCHHHHHHHHCCCCCCEEEEECHHHHHHHHHCCCCCHHHHH
KIIGHVFVDIFDEESKKLKNAKWLAQGTIYPDVIESAASKTGKAHVIKSHHNVGGLPDDM
HHHHHHHHHHHCHHHHHHHCCCEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCHH
EMGLVEPLRELFKDEVRKIGLELGLPYNMLYRHPFPGPGLGVRVLGEIKKEYCDLLRRAD
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
AIFIEELHNADLYNKVSQAFTVFLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHL
HHHHHHHHCCHHHHHHHHHHEEEEEHHHCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHC
PYDFLGKVSNRIINEVNGISRVVYDISGKPPATIEWE
CHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA