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The map label for this gene is gcvA [H]

Identifier: 59711198

GI number: 59711198

Start: 649287

End: 650195

Strand: Direct

Name: gcvA [H]

Synonym: VF_0591

Alternate gene names: 59711198

Gene position: 649287-650195 (Clockwise)

Preceding gene: 59711195

Following gene: 59711199

Centisome position: 22.41

GC content: 39.16

Gene sequence:

>909_bases
ATGTCTCGAAGATTACCACCTTTAAATTCTTTACGTGTGTTTGAAGCGGCAGCCAGGCACTTAAGCTTCACGCGTGCAGC
AGAAGAATTATTTGTTACACAAGCGGCAGTTAGCCACCAAATCAAAGCATTAGAAGAGTTTCTTGGTCTTAAACTTTTTC
GACGCCGAAATCGCTCTTTGTTATTAACAGAAGAGGGACAGAGCTACTTTTTAGACATTAAAGAAATCTTCTCTTCTTTA
TCTGAAGCAACAGATAAAGTATTGGAAAGAAGTGCCAAAGGGGCACTAACTATTAGTTTACCGCCAAGTTTTGCTATTCA
ATGGTTGGTACCAAGATTATCGGATTTTAATAATCAAGAACCAGATATTGATGTTCGTATTAAAGCCGTTGATATGGATG
AAGGTTCATTAACCGAAGATGTGGATGTTGCTATCTATTATGGTCGAGGTAATTGGCCGGGTCTTCGAGTTGAACTGTTA
TATCAAGAACAGTTATTGCCATTATGTTCACCTCAAGTGCTACTGAATGAAAAGCCATTAGCGACCATTGATGATTTACG
TTTCCATACGTTATTGCATGATACTTCTCGTAAAGACTGGAAGCAGTTTGTTAAGCATTACGAATTAGCTGGTATGAATG
TAAACCAAGGACCTATTTTTAGTCACTCGACTATGGTGTTACAAGCTGCTGCTCACGGGCAAGGTATTGCGTTAGGTAAT
AACGTACTAGCTCAACCTGAATTAGATGCGGGTCGTTTGGTTGCCCCATTTGAAGAAGTATTAGTGAGTAAGAATGCTTT
CTATCTTGTATGTAATGAAAAACAGGCTGATACCGGTCGTATTGCAACGTTTAGAGAGTGGATTTTAAGTAAAGCTCGTA
GCGAACAAGAGATTGTGGATGATGAATAG

Upstream 100 bases:

>100_bases
AAACTACGGGGCTAATATTAGGTTTTATCTGGTAGTTCGACAATGGACAAATTATAATACTTGGCATTAAAAAAACTAAT
GGTTCAAAATGTGAGTTCTT

Downstream 100 bases:

>100_bases
TCGTTTATGTTTATTGATTGCGAGTATTAGTGGAGCACTAACTGTTGGGCTAGGGGCATTTGGTGCTCATGGTTTAAAAG
CGTTTTTATCTCCCTATCTA

Product: DNA-binding transcriptional activator GcvA

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MSRRLPPLNSLRVFEAAARHLSFTRAAEELFVTQAAVSHQIKALEEFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSSL
SEATDKVLERSAKGALTISLPPSFAIQWLVPRLSDFNNQEPDIDVRIKAVDMDEGSLTEDVDVAIYYGRGNWPGLRVELL
YQEQLLPLCSPQVLLNEKPLATIDDLRFHTLLHDTSRKDWKQFVKHYELAGMNVNQGPIFSHSTMVLQAAAHGQGIALGN
NVLAQPELDAGRLVAPFEEVLVSKNAFYLVCNEKQADTGRIATFREWILSKARSEQEIVDDE

Sequences:

>Translated_302_residues
MSRRLPPLNSLRVFEAAARHLSFTRAAEELFVTQAAVSHQIKALEEFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSSL
SEATDKVLERSAKGALTISLPPSFAIQWLVPRLSDFNNQEPDIDVRIKAVDMDEGSLTEDVDVAIYYGRGNWPGLRVELL
YQEQLLPLCSPQVLLNEKPLATIDDLRFHTLLHDTSRKDWKQFVKHYELAGMNVNQGPIFSHSTMVLQAAAHGQGIALGN
NVLAQPELDAGRLVAPFEEVLVSKNAFYLVCNEKQADTGRIATFREWILSKARSEQEIVDDE
>Mature_301_residues
SRRLPPLNSLRVFEAAARHLSFTRAAEELFVTQAAVSHQIKALEEFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSSLS
EATDKVLERSAKGALTISLPPSFAIQWLVPRLSDFNNQEPDIDVRIKAVDMDEGSLTEDVDVAIYYGRGNWPGLRVELLY
QEQLLPLCSPQVLLNEKPLATIDDLRFHTLLHDTSRKDWKQFVKHYELAGMNVNQGPIFSHSTMVLQAAAHGQGIALGNN
VLAQPELDAGRLVAPFEEVLVSKNAFYLVCNEKQADTGRIATFREWILSKARSEQEIVDDE

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=297, Percent_Identity=74.4107744107744, Blast_Score=460, Evalue=1e-131,
Organism=Escherichia coli, GI1788706, Length=294, Percent_Identity=31.6326530612245, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1786448, Length=288, Percent_Identity=34.7222222222222, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI145693193, Length=290, Percent_Identity=27.9310344827586, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1786401, Length=291, Percent_Identity=27.4914089347079, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI157672245, Length=212, Percent_Identity=29.7169811320755, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1787879, Length=130, Percent_Identity=34.6153846153846, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI87081978, Length=270, Percent_Identity=27.7777777777778, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1787128, Length=297, Percent_Identity=24.9158249158249, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787601, Length=173, Percent_Identity=28.3236994219653, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790262, Length=237, Percent_Identity=26.1603375527426, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI145693105, Length=122, Percent_Identity=31.1475409836066, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34135; Mature: 34004

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRLPPLNSLRVFEAAARHLSFTRAAEELFVTQAAVSHQIKALEEFLGLKLFRRRNRSL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LLTEEGQSYFLDIKEIFSSLSEATDKVLERSAKGALTISLPPSFAIQWLVPRLSDFNNQE
EEEECCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCCC
PDIDVRIKAVDMDEGSLTEDVDVAIYYGRGNWPGLRVELLYQEQLLPLCSPQVLLNEKPL
CCEEEEEEEEECCCCCCCCCCEEEEEECCCCCCCEEEEEEEHHHCCCCCCCHHEECCCCC
ATIDDLRFHTLLHDTSRKDWKQFVKHYELAGMNVNQGPIFSHSTMVLQAAAHGQGIALGN
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHEEEECCCCCCEEECC
NVLAQPELDAGRLVAPFEEVLVSKNAFYLVCNEKQADTGRIATFREWILSKARSEQEIVD
CEECCCCCCCCCEECCHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCC
DE
CC
>Mature Secondary Structure 
SRRLPPLNSLRVFEAAARHLSFTRAAEELFVTQAAVSHQIKALEEFLGLKLFRRRNRSL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LLTEEGQSYFLDIKEIFSSLSEATDKVLERSAKGALTISLPPSFAIQWLVPRLSDFNNQE
EEEECCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCCC
PDIDVRIKAVDMDEGSLTEDVDVAIYYGRGNWPGLRVELLYQEQLLPLCSPQVLLNEKPL
CCEEEEEEEEECCCCCCCCCCEEEEEECCCCCCCEEEEEEEHHHCCCCCCCHHEECCCCC
ATIDDLRFHTLLHDTSRKDWKQFVKHYELAGMNVNQGPIFSHSTMVLQAAAHGQGIALGN
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHEEEECCCCCCEEECC
NVLAQPELDAGRLVAPFEEVLVSKNAFYLVCNEKQADTGRIATFREWILSKARSEQEIVD
CEECCCCCCCCCEECCHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCC
DE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]