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The map label for this gene is pyrI

Identifier: 59711012

GI number: 59711012

Start: 433746

End: 434210

Strand: Reverse

Name: pyrI

Synonym: VF_0405

Alternate gene names: 59711012

Gene position: 434210-433746 (Counterclockwise)

Preceding gene: 59711013

Following gene: 59711011

Centisome position: 14.99

GC content: 36.77

Gene sequence:

>465_bases
ATGGCTAACAAAACTCAATTACGTGTTGAAGCAATCAACAACGGTACGGTAATCGATCATATTCCCGCTAACATTGGGAT
TAAAGTGCTTAAATTATTCCAAATGGATAAAAGTGCAGAGCGTGTGACTGTTGGTTTGAATTTACCTTCATCAGCGCTTG
GAGCAAAAGATTTATTAAAAATTGAAAATACATTTATCACTCCAGAGCAAGCAAGCAAACTCGCATTATATGCACCGCAC
GCAACAGTTAACCAAATTGAAAACTATGAAGTAGTAAAGAAAATACCATTAGTGCTACCAGAGCAAATCACGGGTGTTTT
TGAGTGTCCAAACAGTAACTGTATTACTCACGGTGAACCGGTAGACAGCAGCTTCAAAGTTCTAACAAAGAAAGAAGATA
TTCATCTGAAGTGTAAATACTGTGAGAAAGTATACTCACGTGAAGTAGTCACTGACTTAAATTAA

Upstream 100 bases:

>100_bases
CCACACGCTTACTATTTCCAACAGGCTGAAAATGGTGTTTATGCTCGCCAAGCTCTATTGGCTTTAGTTTTAAATGAAAC
ACTTTAAGGGAGAGAATACC

Downstream 100 bases:

>100_bases
TTAAATTTGATTCTCTTCTCAGCCTCGATATTCTCGGGGCTGTTTTCTTACTGTTATTTGTTTTATATTGAACAATCGTA
ATGCTTCTAAGGCCTATTGC

Product: aspartate carbamoyltransferase regulatory subunit

Products: phosphate; N-carbamoyl-L-aspartate [C]

Alternate protein names: NA

Number of amino acids: Translated: 154; Mature: 153

Protein sequence:

>154_residues
MANKTQLRVEAINNGTVIDHIPANIGIKVLKLFQMDKSAERVTVGLNLPSSALGAKDLLKIENTFITPEQASKLALYAPH
ATVNQIENYEVVKKIPLVLPEQITGVFECPNSNCITHGEPVDSSFKVLTKKEDIHLKCKYCEKVYSREVVTDLN

Sequences:

>Translated_154_residues
MANKTQLRVEAINNGTVIDHIPANIGIKVLKLFQMDKSAERVTVGLNLPSSALGAKDLLKIENTFITPEQASKLALYAPH
ATVNQIENYEVVKKIPLVLPEQITGVFECPNSNCITHGEPVDSSFKVLTKKEDIHLKCKYCEKVYSREVVTDLN
>Mature_153_residues
ANKTQLRVEAINNGTVIDHIPANIGIKVLKLFQMDKSAERVTVGLNLPSSALGAKDLLKIENTFITPEQASKLALYAPHA
TVNQIENYEVVKKIPLVLPEQITGVFECPNSNCITHGEPVDSSFKVLTKKEDIHLKCKYCEKVYSREVVTDLN

Specific function: Involved in allosteric regulation of aspartate carbamoyltransferase

COG id: COG1781

COG function: function code F; Aspartate carbamoyltransferase, regulatory subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyrI family

Homologues:

Organism=Escherichia coli, GI1790692, Length=150, Percent_Identity=60, Blast_Score=191, Evalue=2e-50,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 440 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRI_VIBF1 (Q5E7U6)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_203788.1
- ProteinModelPortal:   Q5E7U6
- SMR:   Q5E7U6
- STRING:   Q5E7U6
- GeneID:   3278191
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_0405
- NMPDR:   fig|312309.3.peg.405
- eggNOG:   COG1781
- HOGENOM:   HBG305327
- OMA:   EGTVIDH
- PhylomeDB:   Q5E7U6
- ProtClustDB:   PRK00893
- BioCyc:   VFIS312309:VF0405-MONOMER
- HAMAP:   MF_00002
- InterPro:   IPR020545
- InterPro:   IPR002801
- InterPro:   IPR020542
- Gene3D:   G3DSA:2.30.30.20
- Gene3D:   G3DSA:3.30.70.140
- ProDom:   PD006194
- TIGRFAMs:   TIGR00240

Pfam domain/function: PF01948 PyrI; PF02748 PyrI_C; SSF54893 Asp_carbamoylTrfase_reg; SSF57825 Asp_transf_reg_C

EC number: NA

Molecular weight: Translated: 17090; Mature: 16959

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANKTQLRVEAINNGTVIDHIPANIGIKVLKLFQMDKSAERVTVGLNLPSSALGAKDLLK
CCCCCEEEEEECCCCEEEEECCCCCCHHHHHHHHCCCCCCEEEEEECCCCHHCCHHHHHH
IENTFITPEQASKLALYAPHATVNQIENYEVVKKIPLVLPEQITGVFECPNSNCITHGEP
HHCEEECCCCCCEEEEECCCCHHHHHCCHHHHHHCCCCCCCCCCEEEECCCCCCEECCCC
VDSSFKVLTKKEDIHLKCKYCEKVYSREVVTDLN
CCCCEEEEECCCCEEEEEHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ANKTQLRVEAINNGTVIDHIPANIGIKVLKLFQMDKSAERVTVGLNLPSSALGAKDLLK
CCCCEEEEEECCCCEEEEECCCCCCHHHHHHHHCCCCCCEEEEEECCCCHHCCHHHHHH
IENTFITPEQASKLALYAPHATVNQIENYEVVKKIPLVLPEQITGVFECPNSNCITHGEP
HHCEEECCCCCCEEEEECCCCHHHHHCCHHHHHHCCCCCCCCCCEEEECCCCCCEECCCC
VDSSFKVLTKKEDIHLKCKYCEKVYSREVVTDLN
CCCCEEEEECCCCEEEEEHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Zn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: carbamoyl phosphate; L-aspartate [C]

Specific reaction: carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate [C]

General reaction: Transferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA