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The map label for this gene is pyrB [H]

Identifier: 59711013

GI number: 59711013

Start: 434224

End: 435153

Strand: Reverse

Name: pyrB [H]

Synonym: VF_0406

Alternate gene names: 59711013

Gene position: 435153-434224 (Counterclockwise)

Preceding gene: 172087660

Following gene: 59711012

Centisome position: 15.02

GC content: 40.75

Gene sequence:

>930_bases
ATGACTAACTCGCTATATCAAAAGCATATCATTTCTATTCCAGAGCTTTCTCGTCAAGAGTTAGAGCTGATCGTTGAAAC
GGCAGGGAAGATAAAAAAAGAACCTCAACCTGATTTATTGAAAAATAAAATCGTGGCGAGTTGTTTCTTCGAAGCATCAA
CTCGTACCCGTCTTTCATTTGAAACTGCCATCCAGCGTTTAGGTGGTTCAGTAATTGGTTTCGATACCGCTGGCAATACC
TCTCTTGCACAAAAAGGGGAAACGCTAGCGGATTCAGTTCAAATCATTACCTCATACGCAGATGCTTACGTTATGCGTCA
TCCACAAGAAGGAGCTGCACGCTTAGCGTCTGAATTCTCAAACGGCACACCAGTAATTAATGCTGGTGACGGTGCAAACC
AACACCCAACTCAAACGCTCTTAGATCTTTACACCATTTATGAAACTCAAGGTCGCTTAGATAATCTAAACGTCGCTTTC
GTTGGTGATTTGAAATACGGTCGTACTGTCCACTCTCTGACCCAAGCATTAGCAAAATTTGAAGGCATTAAATTCTTCTT
TATTGCTCCTGAAGTATTAGCGATGCCTGACTACATTTGCGAAGAACTTGATGAACTAGGTATTGAGTACCAATTGGTTG
AAAGCATGGATGATGCAATTCCTGAACTTGATATTCTATACATGACTCGAGTACAGAAAGAGCGTTTTGATGAATCTGAA
TACGCACACATCAAGTCAGCATATATTCTTTCAGCTGAAAACTTACAACCTGCACGCGAAAATCTAAAAGTACTGCATCC
ACTACCACGTGTTGATGAAATTGATACCGATGTAGACGCAACGCCACACGCTTACTATTTCCAACAGGCTGAAAATGGTG
TTTATGCTCGCCAAGCTCTATTGGCTTTAGTTTTAAATGAAACACTTTAA

Upstream 100 bases:

>100_bases
CCCTATTTTTTGATTTTCCTAATCAACCCAATTTTTAAAGCCTCCTATTTATTAGGGGGCTTTTTTTTGATTTCAATATA
ATACATAAGGGAAGAGAGAC

Downstream 100 bases:

>100_bases
GGGAGAGAATACCATGGCTAACAAAACTCAATTACGTGTTGAAGCAATCAACAACGGTACGGTAATCGATCATATTCCCG
CTAACATTGGGATTAAAGTG

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MTNSLYQKHIISIPELSRQELELIVETAGKIKKEPQPDLLKNKIVASCFFEASTRTRLSFETAIQRLGGSVIGFDTAGNT
SLAQKGETLADSVQIITSYADAYVMRHPQEGAARLASEFSNGTPVINAGDGANQHPTQTLLDLYTIYETQGRLDNLNVAF
VGDLKYGRTVHSLTQALAKFEGIKFFFIAPEVLAMPDYICEELDELGIEYQLVESMDDAIPELDILYMTRVQKERFDESE
YAHIKSAYILSAENLQPARENLKVLHPLPRVDEIDTDVDATPHAYYFQQAENGVYARQALLALVLNETL

Sequences:

>Translated_309_residues
MTNSLYQKHIISIPELSRQELELIVETAGKIKKEPQPDLLKNKIVASCFFEASTRTRLSFETAIQRLGGSVIGFDTAGNT
SLAQKGETLADSVQIITSYADAYVMRHPQEGAARLASEFSNGTPVINAGDGANQHPTQTLLDLYTIYETQGRLDNLNVAF
VGDLKYGRTVHSLTQALAKFEGIKFFFIAPEVLAMPDYICEELDELGIEYQLVESMDDAIPELDILYMTRVQKERFDESE
YAHIKSAYILSAENLQPARENLKVLHPLPRVDEIDTDVDATPHAYYFQQAENGVYARQALLALVLNETL
>Mature_308_residues
TNSLYQKHIISIPELSRQELELIVETAGKIKKEPQPDLLKNKIVASCFFEASTRTRLSFETAIQRLGGSVIGFDTAGNTS
LAQKGETLADSVQIITSYADAYVMRHPQEGAARLASEFSNGTPVINAGDGANQHPTQTLLDLYTIYETQGRLDNLNVAFV
GDLKYGRTVHSLTQALAKFEGIKFFFIAPEVLAMPDYICEELDELGIEYQLVESMDDAIPELDILYMTRVQKERFDESEY
AHIKSAYILSAENLQPARENLKVLHPLPRVDEIDTDVDATPHAYYFQQAENGVYARQALLALVLNETL

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=311, Percent_Identity=44.6945337620579, Blast_Score=270, Evalue=1e-72,
Organism=Homo sapiens, GI38788445, Length=280, Percent_Identity=28.9285714285714, Blast_Score=75, Evalue=7e-14,
Organism=Escherichia coli, GI2367364, Length=309, Percent_Identity=69.57928802589, Blast_Score=467, Evalue=1e-133,
Organism=Escherichia coli, GI1786469, Length=345, Percent_Identity=25.5072463768116, Blast_Score=61, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI193204318, Length=326, Percent_Identity=41.1042944785276, Blast_Score=221, Evalue=3e-58,
Organism=Saccharomyces cerevisiae, GI6322331, Length=309, Percent_Identity=39.4822006472492, Blast_Score=235, Evalue=7e-63,
Organism=Saccharomyces cerevisiae, GI6322373, Length=326, Percent_Identity=25.4601226993865, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24642586, Length=312, Percent_Identity=42.9487179487179, Blast_Score=258, Evalue=5e-69,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 34525; Mature: 34394

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNSLYQKHIISIPELSRQELELIVETAGKIKKEPQPDLLKNKIVASCFFEASTRTRLSF
CCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHH
ETAIQRLGGSVIGFDTAGNTSLAQKGETLADSVQIITSYADAYVMRHPQEGAARLASEFS
HHHHHHHCCCEEEECCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHC
NGTPVINAGDGANQHPTQTLLDLYTIYETQGRLDNLNVAFVGDLKYGRTVHSLTQALAKF
CCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHH
EGIKFFFIAPEVLAMPDYICEELDELGIEYQLVESMDDAIPELDILYMTRVQKERFDESE
CCCEEEEECCHHHHCCHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHH
YAHIKSAYILSAENLQPARENLKVLHPLPRVDEIDTDVDATPHAYYFQQAENGVYARQAL
HHHHHHHEEEECCCCCCHHHCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHH
LALVLNETL
HHHHHHHCC
>Mature Secondary Structure 
TNSLYQKHIISIPELSRQELELIVETAGKIKKEPQPDLLKNKIVASCFFEASTRTRLSF
CCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHH
ETAIQRLGGSVIGFDTAGNTSLAQKGETLADSVQIITSYADAYVMRHPQEGAARLASEFS
HHHHHHHCCCEEEECCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHC
NGTPVINAGDGANQHPTQTLLDLYTIYETQGRLDNLNVAFVGDLKYGRTVHSLTQALAKF
CCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHH
EGIKFFFIAPEVLAMPDYICEELDELGIEYQLVESMDDAIPELDILYMTRVQKERFDESE
CCCEEEEECCHHHHCCHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHH
YAHIKSAYILSAENLQPARENLKVLHPLPRVDEIDTDVDATPHAYYFQQAENGVYARQAL
HHHHHHHEEEECCCCCCHHHCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHH
LALVLNETL
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA