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The map label for this gene is spoT [H]

Identifier: 59710711

GI number: 59710711

Start: 122442

End: 124565

Strand: Reverse

Name: spoT [H]

Synonym: VF_0104

Alternate gene names: 59710711

Gene position: 124565-122442 (Counterclockwise)

Preceding gene: 59710712

Following gene: 59710710

Centisome position: 4.3

GC content: 43.13

Gene sequence:

>2124_bases
TTGGATTTATTCGATAGCCTGAAAGATGTTGCCAAAGATTACCTGCCAGAGCCTCACATTGAGGCTCTTCGTCGATCTTA
TCTGGTAGCAAAAGAAGCCCATGAAGGGCAAACTCGCTCAAGTGGCGAACCTTATATCATCCACCCAGTAGCTGTGGCTC
GAATCCTTGCAGAAATGAAGCTCGACCACAAAACGCTGATGGCTGCGCTGCTTCATGATGTTATTGAAGACACTGCAGTT
AGCAAAGAAGAGCTCGCTGAGCAGTTTGGTGATACGGTTGCCGAATTAGTGGATGGTGTTTCTAAGCTGGACAAACTTAA
GTTCCGCGATAAAAAAGAAGCACAGGCTGAAAACTTCCGAAAAATGGTCATGGCGATGGTGCAAGACATCCGCGTTATAT
TGATCAAACTTGCTGACCGAACTCATAACATGCGCACGTTGGGTGCTTTACGCCCTGATAAACGTCGCCGCATTGCTCGT
GAAACTTTAGAAATCTTCTCTCCTTTGGCGCATCGCCTTGGTATCCACAATATTAAAACTGAACTTGAAGAGTTAGGTTT
TGAAGCCCTTTACCCTAATCGCTATCGCGTATTAAAAGAAGTGGTGAAGTCAGCTCGTGGTAACCGTAAAGAGATGATCC
AAAAAATTCACTCTGAAATTGAAGGTCGTATTGCTGAAGCTGGCATCACTGGCCGAGTGATTGGTAGAGAGAAAAACCTG
TTCGCTATTTACAATAAAATGAAAAATAAAGAACAGCGTTTCCATACCATTATGGATATCTATGCGTTCCGAGTAATTAC
CGATAATGTCGATACTTGTTATCGAGCATTAGGCCAAGTTCACAGCATTTACAAACCTCGTCCGGGTCGTATTAAAGACT
ATATTGCTGTACCAAAAGCCAATGGCTACCAATCGCTTCATACCTCTATGGTTGGCCCTCACGGTGTTCCTGTTGAAGTA
CAAATTCGTACTGAAGATATGGAGCAAATGGCGAACAAAGGTGTTGCTGCACATTGGTCGTACAAAGAAGGCGATAACTC
TAGCACAACGGCTCAAATCCGAGCACAACGCTGGATGCAAAGCTTACTTGAACTTCAACAAAGTGCAGGTAGCTCATTTG
AATTTATTGAAAACGTAAAATCGGATCTCTTCCCGAACGAAATTTACGTTTTCACTCCAAAAGGCCGTATTTTTGAACTG
CCTGTTGGCGCAACGGCTGTAGATTTTGCTTACGCCGTTCACTCTGATGTTGGTAACAGTTGTGTTGGTTCACGTGTTAA
CCGTCAACCTTACCCACTAAGTAAACCACTTAAAAATGGTCAGACGGTTGAAATCATCAGTGCTCCTGGTGCTCGTCCAA
ATGCAGCATGGCTGAACTACGTAGTAACATCAAAAGCACGTACGAAGATCCGCCAAGTGCTAAAAACCATGCGCAGAGAA
GACTCTGTAGTATTAGGTCGTCGCCTATTGAATCACGCTTTAGGTCATAACGTATCACTAGACGATATATCTCAAGAGAA
TATTGATCACGTTTTATCTGATCTTAAATTAGCAGAACTGGATGACTTACTGGCCGCTATCGGCTTAGGTGAGTTGATGA
GTATTGTTATTGCTCGTCGCCTATTAGGCTCTGCTGATGAATTAACGGAACAAACCTCAAACATTGAAACCACCACTGGT
AAAAAACTACCAATCCGTGGTGCTGATGGCATCTTACTGACATTTGCAAACTGTTGTCGCCCTATTCCGGGTGATCACAT
TATTGCTCACGTAAGTCCTGGCCGTGGCCTTGTGGTTCATATGGAAGAGTGTGCAAATGTTCGTGGTTATCAAAAAGAAC
TTGATAAGTACATGGGCGTTGAATGGGCTGATGAATTCGATCAAGAATTTGATACGGCATTAGATATTGATATCCAAAAC
CACCAAGGTGCATTAGCTGATTTGGCGAATGTGATTGCAGGTACAGGATCAAATATCCGTGGTATTTCAACAGAAGAAAA
AGATGGACGCTTATATACAGTAACCATCGAGCTTACGGTGAAAGATCGTAAACATTTAGCTGAAATAATGCGTCGTATCC
GTGTTATGCCACACGCACTTAAAGTACGTAGACATAAGAACTAG

Upstream 100 bases:

>100_bases
AAGACGCAGCTGAATTAGCAGCAGTAAGCTCAATCACACACAATCGTTAATCTTTATAATCTCTTGTAGATTGTAAACAG
CAAACCTGACGGGGCGAAAT

Downstream 100 bases:

>100_bases
ATGCGAGGGACGAGGTTCGAGTACGGACCTCGTCCTTTTAGTTAGATCTATACACTACGCTAACTAGAACGCTTCGCTCT
AGAAACTATAAAATCAAAAG

Product: bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 707; Mature: 707

Protein sequence:

>707_residues
MDLFDSLKDVAKDYLPEPHIEALRRSYLVAKEAHEGQTRSSGEPYIIHPVAVARILAEMKLDHKTLMAALLHDVIEDTAV
SKEELAEQFGDTVAELVDGVSKLDKLKFRDKKEAQAENFRKMVMAMVQDIRVILIKLADRTHNMRTLGALRPDKRRRIAR
ETLEIFSPLAHRLGIHNIKTELEELGFEALYPNRYRVLKEVVKSARGNRKEMIQKIHSEIEGRIAEAGITGRVIGREKNL
FAIYNKMKNKEQRFHTIMDIYAFRVITDNVDTCYRALGQVHSIYKPRPGRIKDYIAVPKANGYQSLHTSMVGPHGVPVEV
QIRTEDMEQMANKGVAAHWSYKEGDNSSTTAQIRAQRWMQSLLELQQSAGSSFEFIENVKSDLFPNEIYVFTPKGRIFEL
PVGATAVDFAYAVHSDVGNSCVGSRVNRQPYPLSKPLKNGQTVEIISAPGARPNAAWLNYVVTSKARTKIRQVLKTMRRE
DSVVLGRRLLNHALGHNVSLDDISQENIDHVLSDLKLAELDDLLAAIGLGELMSIVIARRLLGSADELTEQTSNIETTTG
KKLPIRGADGILLTFANCCRPIPGDHIIAHVSPGRGLVVHMEECANVRGYQKELDKYMGVEWADEFDQEFDTALDIDIQN
HQGALADLANVIAGTGSNIRGISTEEKDGRLYTVTIELTVKDRKHLAEIMRRIRVMPHALKVRRHKN

Sequences:

>Translated_707_residues
MDLFDSLKDVAKDYLPEPHIEALRRSYLVAKEAHEGQTRSSGEPYIIHPVAVARILAEMKLDHKTLMAALLHDVIEDTAV
SKEELAEQFGDTVAELVDGVSKLDKLKFRDKKEAQAENFRKMVMAMVQDIRVILIKLADRTHNMRTLGALRPDKRRRIAR
ETLEIFSPLAHRLGIHNIKTELEELGFEALYPNRYRVLKEVVKSARGNRKEMIQKIHSEIEGRIAEAGITGRVIGREKNL
FAIYNKMKNKEQRFHTIMDIYAFRVITDNVDTCYRALGQVHSIYKPRPGRIKDYIAVPKANGYQSLHTSMVGPHGVPVEV
QIRTEDMEQMANKGVAAHWSYKEGDNSSTTAQIRAQRWMQSLLELQQSAGSSFEFIENVKSDLFPNEIYVFTPKGRIFEL
PVGATAVDFAYAVHSDVGNSCVGSRVNRQPYPLSKPLKNGQTVEIISAPGARPNAAWLNYVVTSKARTKIRQVLKTMRRE
DSVVLGRRLLNHALGHNVSLDDISQENIDHVLSDLKLAELDDLLAAIGLGELMSIVIARRLLGSADELTEQTSNIETTTG
KKLPIRGADGILLTFANCCRPIPGDHIIAHVSPGRGLVVHMEECANVRGYQKELDKYMGVEWADEFDQEFDTALDIDIQN
HQGALADLANVIAGTGSNIRGISTEEKDGRLYTVTIELTVKDRKHLAEIMRRIRVMPHALKVRRHKN
>Mature_707_residues
MDLFDSLKDVAKDYLPEPHIEALRRSYLVAKEAHEGQTRSSGEPYIIHPVAVARILAEMKLDHKTLMAALLHDVIEDTAV
SKEELAEQFGDTVAELVDGVSKLDKLKFRDKKEAQAENFRKMVMAMVQDIRVILIKLADRTHNMRTLGALRPDKRRRIAR
ETLEIFSPLAHRLGIHNIKTELEELGFEALYPNRYRVLKEVVKSARGNRKEMIQKIHSEIEGRIAEAGITGRVIGREKNL
FAIYNKMKNKEQRFHTIMDIYAFRVITDNVDTCYRALGQVHSIYKPRPGRIKDYIAVPKANGYQSLHTSMVGPHGVPVEV
QIRTEDMEQMANKGVAAHWSYKEGDNSSTTAQIRAQRWMQSLLELQQSAGSSFEFIENVKSDLFPNEIYVFTPKGRIFEL
PVGATAVDFAYAVHSDVGNSCVGSRVNRQPYPLSKPLKNGQTVEIISAPGARPNAAWLNYVVTSKARTKIRQVLKTMRRE
DSVVLGRRLLNHALGHNVSLDDISQENIDHVLSDLKLAELDDLLAAIGLGELMSIVIARRLLGSADELTEQTSNIETTTG
KKLPIRGADGILLTFANCCRPIPGDHIIAHVSPGRGLVVHMEECANVRGYQKELDKYMGVEWADEFDQEFDTALDIDIQN
HQGALADLANVIAGTGSNIRGISTEEKDGRLYTVTIELTVKDRKHLAEIMRRIRVMPHALKVRRHKN

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance [H]

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=707, Percent_Identity=73.1258840169731, Blast_Score=1085, Evalue=0.0,
Organism=Escherichia coli, GI1789147, Length=687, Percent_Identity=31.7321688500728, Blast_Score=386, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI25143118, Length=139, Percent_Identity=33.8129496402878, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 79586; Mature: 79586

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLFDSLKDVAKDYLPEPHIEALRRSYLVAKEAHEGQTRSSGEPYIIHPVAVARILAEMK
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECHHHHHHHHHHHH
LDHKTLMAALLHDVIEDTAVSKEELAEQFGDTVAELVDGVSKLDKLKFRDKKEAQAENFR
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
KMVMAMVQDIRVILIKLADRTHNMRTLGALRPDKRRRIARETLEIFSPLAHRLGIHNIKT
HHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHH
ELEELGFEALYPNRYRVLKEVVKSARGNRKEMIQKIHSEIEGRIAEAGITGRVIGREKNL
HHHHCCCHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHCCCCEEEECCCCCH
FAIYNKMKNKEQRFHTIMDIYAFRVITDNVDTCYRALGQVHSIYKPRPGRIKDYIAVPKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCCC
NGYQSLHTSMVGPHGVPVEVQIRTEDMEQMANKGVAAHWSYKEGDNSSTTAQIRAQRWMQ
CCHHHHHHHHCCCCCCCEEEEECHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHH
SLLELQQSAGSSFEFIENVKSDLFPNEIYVFTPKGRIFELPVGATAVDFAYAVHSDVGNS
HHHHHHHHCCCCHHHHHHHHHHCCCCCEEEECCCCEEEECCCCCHHHHHHHHHHHHCCCH
CVGSRVNRQPYPLSKPLKNGQTVEIISAPGARPNAAWLNYVVTSKARTKIRQVLKTMRRE
HHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
DSVVLGRRLLNHALGHNVSLDDISQENIDHVLSDLKLAELDDLLAAIGLGELMSIVIARR
CHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
LLGSADELTEQTSNIETTTGKKLPIRGADGILLTFANCCRPIPGDHIIAHVSPGRGLVVH
HHCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCEEEE
MEECANVRGYQKELDKYMGVEWADEFDQEFDTALDIDIQNHQGALADLANVIAGTGSNIR
HHHHCCCCHHHHHHHHHHCCCHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHCCCCCCC
GISTEEKDGRLYTVTIELTVKDRKHLAEIMRRIRVMPHALKVRRHKN
CCCCCCCCCEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDLFDSLKDVAKDYLPEPHIEALRRSYLVAKEAHEGQTRSSGEPYIIHPVAVARILAEMK
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECHHHHHHHHHHHH
LDHKTLMAALLHDVIEDTAVSKEELAEQFGDTVAELVDGVSKLDKLKFRDKKEAQAENFR
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
KMVMAMVQDIRVILIKLADRTHNMRTLGALRPDKRRRIARETLEIFSPLAHRLGIHNIKT
HHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHH
ELEELGFEALYPNRYRVLKEVVKSARGNRKEMIQKIHSEIEGRIAEAGITGRVIGREKNL
HHHHCCCHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHCCCCEEEECCCCCH
FAIYNKMKNKEQRFHTIMDIYAFRVITDNVDTCYRALGQVHSIYKPRPGRIKDYIAVPKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCCC
NGYQSLHTSMVGPHGVPVEVQIRTEDMEQMANKGVAAHWSYKEGDNSSTTAQIRAQRWMQ
CCHHHHHHHHCCCCCCCEEEEECHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHH
SLLELQQSAGSSFEFIENVKSDLFPNEIYVFTPKGRIFELPVGATAVDFAYAVHSDVGNS
HHHHHHHHCCCCHHHHHHHHHHCCCCCEEEECCCCEEEECCCCCHHHHHHHHHHHHCCCH
CVGSRVNRQPYPLSKPLKNGQTVEIISAPGARPNAAWLNYVVTSKARTKIRQVLKTMRRE
HHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
DSVVLGRRLLNHALGHNVSLDDISQENIDHVLSDLKLAELDDLLAAIGLGELMSIVIARR
CHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
LLGSADELTEQTSNIETTTGKKLPIRGADGILLTFANCCRPIPGDHIIAHVSPGRGLVVH
HHCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCEEEE
MEECANVRGYQKELDKYMGVEWADEFDQEFDTALDIDIQNHQGALADLANVIAGTGSNIR
HHHHCCCCHHHHHHHHHHCCCHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHCCCCCCC
GISTEEKDGRLYTVTIELTVKDRKHLAEIMRRIRVMPHALKVRRHKN
CCCCCCCCCEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]