Click here to switch to the map view.

The map label for this gene is folD

Identifier: 58617425

GI number: 58617425

Start: 1107929

End: 1108831

Strand: Reverse

Name: folD

Synonym: ERGA_CDS_06980

Alternate gene names: 58617425

Gene position: 1108831-1107929 (Counterclockwise)

Preceding gene: 58617426

Following gene: 58617424

Centisome position: 73.93

GC content: 34.99

Gene sequence:

>903_bases
ATGGAAGGGAATATTGTAAGTGGGAAAGCAGTTGCTGATAATATTACAAACATACTTGCTACATGTATTAGTGATTTAAA
AGTACAGCATAATTTAACACCGTGTTTAATAGTTGTTCTAGTTGGAGATGATCCTGCAAGTCAATTGTATGTGCGTAATA
AGCAACGAAAAGCAGAAATGTTAGGGCTTAGATCTGAAACGATGTTGCTTCCTAGTACAATATCTGAAAGTAGTTTGATT
GAAAAAATACATCAGCTTAATAATGATGATAGTGTTCATGGAATTTTAGTACAGTTACCTGTTCCGAGACATATTGATAA
GAACCTGATTATCAATACTATTGATCCTAAGAAAGATGTTGATGGATTCCATAATGAAAACGTAGGTAGATTATTTACTG
GACAGAAAAAGAATTGCTTAGTACCTTGTACACCGCAGGGTTGTCTCTATTTAATAAAAACTATCACTCGTAATTTATCT
GGTAGTGATGCTGTAGTTATAGGAAGATCAAATATAGTAGGGAAGCCTATGGCTTGTTTACTATTAGGGGAAAATTGTAC
AGTTACTACTGTTCATTCGGCTACTCGTGATTTGCCAGATTATTGTAGAAGAGCTGATATTCTTGTTGCTGCGGTTGGGA
TACCTCGTTTTGTTAAATACTCTTGGGTAAAACATGGGGCAATTGTTATAGATGTAGGTATTAACTCTATAGAAGAAGAT
GGCGTGAAAAAGTTTGTAGGAGATGTCGATTTTGCTGAGGTCAATAAAATTGCTTCAGCCATTACTCCAGTACCTGGTGG
TGTTGGACCTATGACTATTGCGTTTTTAATGGTTAATACCATTATTGCTGCATGTAACCAGTCAGGAATTCATGGTTTTC
TTGAAAAGTATCTTGATTTATAA

Upstream 100 bases:

>100_bases
TCTTATTTGTAGTGGTTATAAAAATATATTCTTAATGTTTATTGAAGTTTTTTGTAAAATGCACTAACCTTTAAGTTATA
CAATAAGATATTGAACTCTT

Downstream 100 bases:

>100_bases
TTCTTTGATTGCAGTTAACGTATTTATAGTTTATAAAGATAATGTAATAGGGCGATTAGCTCAGTTGGTAGAGCATCTCG
TTTACACCGAGGAGGTCAGC

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MEGNIVSGKAVADNITNILATCISDLKVQHNLTPCLIVVLVGDDPASQLYVRNKQRKAEMLGLRSETMLLPSTISESSLI
EKIHQLNNDDSVHGILVQLPVPRHIDKNLIINTIDPKKDVDGFHNENVGRLFTGQKKNCLVPCTPQGCLYLIKTITRNLS
GSDAVVIGRSNIVGKPMACLLLGENCTVTTVHSATRDLPDYCRRADILVAAVGIPRFVKYSWVKHGAIVIDVGINSIEED
GVKKFVGDVDFAEVNKIASAITPVPGGVGPMTIAFLMVNTIIAACNQSGIHGFLEKYLDL

Sequences:

>Translated_300_residues
MEGNIVSGKAVADNITNILATCISDLKVQHNLTPCLIVVLVGDDPASQLYVRNKQRKAEMLGLRSETMLLPSTISESSLI
EKIHQLNNDDSVHGILVQLPVPRHIDKNLIINTIDPKKDVDGFHNENVGRLFTGQKKNCLVPCTPQGCLYLIKTITRNLS
GSDAVVIGRSNIVGKPMACLLLGENCTVTTVHSATRDLPDYCRRADILVAAVGIPRFVKYSWVKHGAIVIDVGINSIEED
GVKKFVGDVDFAEVNKIASAITPVPGGVGPMTIAFLMVNTIIAACNQSGIHGFLEKYLDL
>Mature_300_residues
MEGNIVSGKAVADNITNILATCISDLKVQHNLTPCLIVVLVGDDPASQLYVRNKQRKAEMLGLRSETMLLPSTISESSLI
EKIHQLNNDDSVHGILVQLPVPRHIDKNLIINTIDPKKDVDGFHNENVGRLFTGQKKNCLVPCTPQGCLYLIKTITRNLS
GSDAVVIGRSNIVGKPMACLLLGENCTVTTVHSATRDLPDYCRRADILVAAVGIPRFVKYSWVKHGAIVIDVGINSIEED
GVKKFVGDVDFAEVNKIASAITPVPGGVGPMTIAFLMVNTIIAACNQSGIHGFLEKYLDL

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=301, Percent_Identity=45.1827242524917, Blast_Score=239, Evalue=2e-63,
Organism=Homo sapiens, GI222418558, Length=295, Percent_Identity=44.7457627118644, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI94721354, Length=295, Percent_Identity=45.4237288135593, Blast_Score=217, Evalue=9e-57,
Organism=Homo sapiens, GI36796743, Length=200, Percent_Identity=28, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1786741, Length=283, Percent_Identity=47.3498233215548, Blast_Score=239, Evalue=1e-64,
Organism=Caenorhabditis elegans, GI17568735, Length=290, Percent_Identity=42.0689655172414, Blast_Score=222, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6319558, Length=288, Percent_Identity=46.5277777777778, Blast_Score=260, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6321643, Length=299, Percent_Identity=42.1404682274247, Blast_Score=223, Evalue=3e-59,
Organism=Saccharomyces cerevisiae, GI6322933, Length=322, Percent_Identity=26.7080745341615, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI62472483, Length=300, Percent_Identity=45.3333333333333, Blast_Score=234, Evalue=4e-62,
Organism=Drosophila melanogaster, GI45551871, Length=300, Percent_Identity=45.3333333333333, Blast_Score=234, Evalue=4e-62,
Organism=Drosophila melanogaster, GI24645718, Length=300, Percent_Identity=45.3333333333333, Blast_Score=234, Evalue=6e-62,
Organism=Drosophila melanogaster, GI17137370, Length=300, Percent_Identity=45.3333333333333, Blast_Score=234, Evalue=6e-62,
Organism=Drosophila melanogaster, GI17136816, Length=297, Percent_Identity=44.4444444444444, Blast_Score=228, Evalue=3e-60,
Organism=Drosophila melanogaster, GI17136818, Length=297, Percent_Identity=44.4444444444444, Blast_Score=228, Evalue=4e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_EHRRG (Q5FG00)

Other databases:

- EMBL:   CR925677
- RefSeq:   YP_196624.1
- HSSP:   P11586
- ProteinModelPortal:   Q5FG00
- SMR:   Q5FG00
- STRING:   Q5FG00
- GeneID:   3268399
- GenomeReviews:   CR925677_GR
- KEGG:   erg:ERGA_CDS_06980
- eggNOG:   COG0190
- HOGENOM:   HBG328751
- OMA:   CLMMLRD
- ProtClustDB:   PRK14187
- BioCyc:   ERUM302409:ERGA_CDS_06980-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 32475; Mature: 32475

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGNIVSGKAVADNITNILATCISDLKVQHNLTPCLIVVLVGDDPASQLYVRNKQRKAEM
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHH
LGLRSETMLLPSTISESSLIEKIHQLNNDDSVHGILVQLPVPRHIDKNLIINTIDPKKDV
HCCCCCCEECCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCEEECCCCCHHC
DGFHNENVGRLFTGQKKNCLVPCTPQGCLYLIKTITRNLSGSDAVVIGRSNIVGKPMACL
CCCCCCCCCCEECCCCCCEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEE
LLGENCTVTTVHSATRDLPDYCRRADILVAAVGIPRFVKYSWVKHGAIVIDVGINSIEED
EECCCCEEEEECHHHCCHHHHHHHHHHEEEECCCHHHHHHHHHHCCEEEEEECCCCHHHH
GVKKFVGDVDFAEVNKIASAITPVPGGVGPMTIAFLMVNTIIAACNQSGIHGFLEKYLDL
HHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure
MEGNIVSGKAVADNITNILATCISDLKVQHNLTPCLIVVLVGDDPASQLYVRNKQRKAEM
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHH
LGLRSETMLLPSTISESSLIEKIHQLNNDDSVHGILVQLPVPRHIDKNLIINTIDPKKDV
HCCCCCCEECCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCEEECCCCCHHC
DGFHNENVGRLFTGQKKNCLVPCTPQGCLYLIKTITRNLSGSDAVVIGRSNIVGKPMACL
CCCCCCCCCCEECCCCCCEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEE
LLGENCTVTTVHSATRDLPDYCRRADILVAAVGIPRFVKYSWVKHGAIVIDVGINSIEED
EECCCCEEEEECHHHCCHHHHHHHHHHEEEECCCHHHHHHHHHHCCEEEEEECCCCHHHH
GVKKFVGDVDFAEVNKIASAITPVPGGVGPMTIAFLMVNTIIAACNQSGIHGFLEKYLDL
HHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA