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The map label for this gene is putP [H]

Identifier: 57238525

GI number: 57238525

Start: 1572627

End: 1574096

Strand: Reverse

Name: putP [H]

Synonym: CJE1675

Alternate gene names: 57238525

Gene position: 1574096-1572627 (Counterclockwise)

Preceding gene: 57238526

Following gene: 57238521

Centisome position: 88.54

GC content: 34.49

Gene sequence:

>1470_bases
ATGGAAGTGGTTCAAATTAATACCCCAATAGCAGTGATGTTTGTAGTGTATGCCTTATTAATGCTTTATATAGGTTTTTA
TTTTTATAAGCAAAATAAAAATTCAGAGGATTATTTTCTAGGTGGGCGTACTATGGGGCCTGTGATATCTGCACTTAGCG
CAGGGGCTTCTGATATGAGTGGTTGGCTTTTAATGGGTTTACCGGGAGCTTTATATGTAAGTGGTTTTATAGATTCTTAT
ATTGCAATTGGGCTTACTATAGGAGCTAGTTTAAATTGGATTTTTGTGGCAAAAAGACTTAGAATTTATACCAGTGTGAT
TGCAAATTCTTTGACTATTCCTGATTATTTTGAAACTCGTTTTGATGATGATAAACATATACTGCGCATCGTATGTGCGG
TGGTAATTTTGATATTTTTTACCTTTTATGTTTCATCAGGGCTTGTAAGCGGAGCAAAACTTTTTGAAAGTACTTTTGGT
ATCAGATACGATTATGCTTTAACTACTGGAACGATTATTATTGTTGCTTATACTTTTTTAGGTGGATATAAGGCGGTTTG
TTGGACGGATATGATACAAGGACTTTTAATGATGATAGCTTTAATTATTGTACCTTTGGTTATGCTTTATCATTTAGGTG
GCTTTGGTGAAGCGATGAATATAGTAAAAGAAATAAAACCACAAGCTTTATCTATGGGCGAAGGAGTAGGAGTTATAAGT
ATTATATCTGCACTTGCTTGGGGTTTGGGTTATTTTGGTCAACCACATATTTTAGTGCGTTTTATGTCGATTCGTTCTAC
TAAAGATATTCCTATGGCCACTTTTATAGGTATTGCTTGGATGGCGGTTTGTCTTTTAAGTGCTTGTATGATAGGTATTT
TGGGCATAGCTTATGTGCATAAATTTGAATTAAGTTTGCAAGATCCTGAAAAGATTTTCATCGTGATGTCGCAGCTTTTA
TTTAATCCTTGGATAGCAGGTATTTTACTCAGTGCTATTTTAGCGGCTATTATGAGTACGGCAAGTTCACAATTGCTGGT
TTCAAGTTCTACTATAGCGGAAGATTTTTATAAAAAGATTTTTAGAGAAGATGCACCAAGTCATATTGTATTAAATTTAG
GTAAATTAGGTGTTTTATTGGTAGCTGTAATAGCGTTTTTGATTTCTACTGATAAAAACTCAAGTGTTTTGAGTATAGTT
TCTTATGCATGGGCTGGATTTGGAGCTTCTTTTGGTTCTGTAATGCTTTTTTCTTTATTTTGGAGTAGGATGACAAGAGT
AGGAGCGATCTTAGGTATGATAACAGGAGCTGCTACTGTGGTGCTTTGGAAGAATTTTGCAAATTCAGGTCTTTATGAAA
TAGTTCCAGGGTTTTTAGCAGCTTCGGCGGTGATTATTATTGCAAGTTTGTTTACAAATGTAAGATCTGGAACTAAAGCA
GCTTATGAAAAGATGTTAAAAGAGCTTTGA

Upstream 100 bases:

>100_bases
AACTTTTAAATTATTTTAATGAAAAAGCTTTAAGTATATCTTTTCATCGTTATGGAAATTTAGGCATTAGAGCAATATCT
TAATGATCAAGGAGAGATTG

Downstream 100 bases:

>100_bases
GAACAAAAGCCTTTTTTAAGGCTTTTGTATTAAAAATCTTAGCGTGGGTCCACTTTGATCGATTTCAAGCACTTTAAAAC
CACGGTTTTTTACATCTTGA

Product: sodium/proline permease

Products: NA

Alternate protein names: Proline permease; Propionate transporter [H]

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MEVVQINTPIAVMFVVYALLMLYIGFYFYKQNKNSEDYFLGGRTMGPVISALSAGASDMSGWLLMGLPGALYVSGFIDSY
IAIGLTIGASLNWIFVAKRLRIYTSVIANSLTIPDYFETRFDDDKHILRIVCAVVILIFFTFYVSSGLVSGAKLFESTFG
IRYDYALTTGTIIIVAYTFLGGYKAVCWTDMIQGLLMMIALIIVPLVMLYHLGGFGEAMNIVKEIKPQALSMGEGVGVIS
IISALAWGLGYFGQPHILVRFMSIRSTKDIPMATFIGIAWMAVCLLSACMIGILGIAYVHKFELSLQDPEKIFIVMSQLL
FNPWIAGILLSAILAAIMSTASSQLLVSSSTIAEDFYKKIFREDAPSHIVLNLGKLGVLLVAVIAFLISTDKNSSVLSIV
SYAWAGFGASFGSVMLFSLFWSRMTRVGAILGMITGAATVVLWKNFANSGLYEIVPGFLAASAVIIIASLFTNVRSGTKA
AYEKMLKEL

Sequences:

>Translated_489_residues
MEVVQINTPIAVMFVVYALLMLYIGFYFYKQNKNSEDYFLGGRTMGPVISALSAGASDMSGWLLMGLPGALYVSGFIDSY
IAIGLTIGASLNWIFVAKRLRIYTSVIANSLTIPDYFETRFDDDKHILRIVCAVVILIFFTFYVSSGLVSGAKLFESTFG
IRYDYALTTGTIIIVAYTFLGGYKAVCWTDMIQGLLMMIALIIVPLVMLYHLGGFGEAMNIVKEIKPQALSMGEGVGVIS
IISALAWGLGYFGQPHILVRFMSIRSTKDIPMATFIGIAWMAVCLLSACMIGILGIAYVHKFELSLQDPEKIFIVMSQLL
FNPWIAGILLSAILAAIMSTASSQLLVSSSTIAEDFYKKIFREDAPSHIVLNLGKLGVLLVAVIAFLISTDKNSSVLSIV
SYAWAGFGASFGSVMLFSLFWSRMTRVGAILGMITGAATVVLWKNFANSGLYEIVPGFLAASAVIIIASLFTNVRSGTKA
AYEKMLKEL
>Mature_489_residues
MEVVQINTPIAVMFVVYALLMLYIGFYFYKQNKNSEDYFLGGRTMGPVISALSAGASDMSGWLLMGLPGALYVSGFIDSY
IAIGLTIGASLNWIFVAKRLRIYTSVIANSLTIPDYFETRFDDDKHILRIVCAVVILIFFTFYVSSGLVSGAKLFESTFG
IRYDYALTTGTIIIVAYTFLGGYKAVCWTDMIQGLLMMIALIIVPLVMLYHLGGFGEAMNIVKEIKPQALSMGEGVGVIS
IISALAWGLGYFGQPHILVRFMSIRSTKDIPMATFIGIAWMAVCLLSACMIGILGIAYVHKFELSLQDPEKIFIVMSQLL
FNPWIAGILLSAILAAIMSTASSQLLVSSSTIAEDFYKKIFREDAPSHIVLNLGKLGVLLVAVIAFLISTDKNSSVLSIV
SYAWAGFGASFGSVMLFSLFWSRMTRVGAILGMITGAATVVLWKNFANSGLYEIVPGFLAASAVIIIASLFTNVRSGTKA
AYEKMLKEL

Specific function: Catalyzes the sodium-dependent uptake of extracellular L-proline. This protein is also capable of using lithium as the transport cation. Also catalyzes the uptake of propionate [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI310128183, Length=474, Percent_Identity=48.1012658227848, Blast_Score=436, Evalue=1e-122,
Organism=Homo sapiens, GI256985183, Length=345, Percent_Identity=25.2173913043478, Blast_Score=76, Evalue=8e-14,
Organism=Homo sapiens, GI4507035, Length=379, Percent_Identity=22.9551451187335, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1787251, Length=487, Percent_Identity=51.7453798767967, Blast_Score=487, Evalue=1e-139,
Organism=Escherichia coli, GI87082237, Length=488, Percent_Identity=26.844262295082, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1790503, Length=457, Percent_Identity=23.8512035010941, Blast_Score=113, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17539284, Length=412, Percent_Identity=25, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24645928, Length=387, Percent_Identity=26.8733850129199, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI221459588, Length=397, Percent_Identity=26.9521410579345, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI221459584, Length=386, Percent_Identity=25.1295336787565, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI221459586, Length=420, Percent_Identity=24.2857142857143, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24651741, Length=460, Percent_Identity=23.9130434782609, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI221459582, Length=415, Percent_Identity=26.2650602409639, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24648033, Length=404, Percent_Identity=21.7821782178218, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI21356865, Length=404, Percent_Identity=21.7821782178218, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI281362918, Length=219, Percent_Identity=27.8538812785388, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 53303; Mature: 53303

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: PS00457 NA_SOLUT_SYMP_2 ; PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVVQINTPIAVMFVVYALLMLYIGFYFYKQNKNSEDYFLGGRTMGPVISALSAGASDMS
CCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHCCCCCCC
GWLLMGLPGALYVSGFIDSYIAIGLTIGASLNWIFVAKRLRIYTSVIANSLTIPDYFETR
CEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHC
FDDDKHILRIVCAVVILIFFTFYVSSGLVSGAKLFESTFGIRYDYALTTGTIIIVAYTFL
CCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHH
GGYKAVCWTDMIQGLLMMIALIIVPLVMLYHLGGFGEAMNIVKEIKPQALSMGEGVGVIS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHCCCCCHHHH
IISALAWGLGYFGQPHILVRFMSIRSTKDIPMATFIGIAWMAVCLLSACMIGILGIAYVH
HHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KFELSLQDPEKIFIVMSQLLFNPWIAGILLSAILAAIMSTASSQLLVSSSTIAEDFYKKI
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
FREDAPSHIVLNLGKLGVLLVAVIAFLISTDKNSSVLSIVSYAWAGFGASFGSVMLFSLF
HHCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHH
WSRMTRVGAILGMITGAATVVLWKNFANSGLYEIVPGFLAASAVIIIASLFTNVRSGTKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
AYEKMLKEL
HHHHHHHHC
>Mature Secondary Structure
MEVVQINTPIAVMFVVYALLMLYIGFYFYKQNKNSEDYFLGGRTMGPVISALSAGASDMS
CCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHCCCCCCC
GWLLMGLPGALYVSGFIDSYIAIGLTIGASLNWIFVAKRLRIYTSVIANSLTIPDYFETR
CEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHC
FDDDKHILRIVCAVVILIFFTFYVSSGLVSGAKLFESTFGIRYDYALTTGTIIIVAYTFL
CCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHH
GGYKAVCWTDMIQGLLMMIALIIVPLVMLYHLGGFGEAMNIVKEIKPQALSMGEGVGVIS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHCCCCCHHHH
IISALAWGLGYFGQPHILVRFMSIRSTKDIPMATFIGIAWMAVCLLSACMIGILGIAYVH
HHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KFELSLQDPEKIFIVMSQLLFNPWIAGILLSAILAAIMSTASSQLLVSSSTIAEDFYKKI
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
FREDAPSHIVLNLGKLGVLLVAVIAFLISTDKNSSVLSIVSYAWAGFGASFGSVMLFSLF
HHCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHH
WSRMTRVGAILGMITGAATVVLWKNFANSGLYEIVPGFLAASAVIIIASLFTNVRSGTKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
AYEKMLKEL
HHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3302614; 8905232; 9278503; 1400239; 3325781; 3053687; 3053649; 1567896; 8119910 [H]