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The map label for this gene is purA [H]

Identifier: 57238521

GI number: 57238521

Start: 1569657

End: 1570907

Strand: Reverse

Name: purA [H]

Synonym: CJE1671

Alternate gene names: 57238521

Gene position: 1570907-1569657 (Counterclockwise)

Preceding gene: 57238525

Following gene: 57238520

Centisome position: 88.36

GC content: 35.01

Gene sequence:

>1251_bases
ATGAGCAAAGCAGATATTATTGTCGGCATTCAATGGGGCGATGAAGGAAAAGGTAAGGTAGTTGATAAATTATGTGAAAA
TTATGATTTTGTTTGCAGAAGTGCAGGTGGACACAATGCAGGACATACGATTTGGGTAAATGGTGTAAGATATGCGCTTC
ATCTTATGCCAAGTGGAGTTTTGCATCCAAGATGTATTAATATTATCGGAAATGGAGTTGTGGTTTCTCCTGAAGTATTG
ATTGCTGAAATGGCTCAATTTGAAAATTTAAAAGGTAGATTATATATCAGCGATAGAGCTCATTTAAATTTAAAACATCA
TTCTTTAATAGACATTGCAAAAGAAAAGCTCAAAGGCAAAAATGCCATAGGAACAACAGGTAAGGGTATAGGACCTTCTT
ATGCAGATAAGATTAATCGTACAGGACATAGAGTTGGAGAGCTTTTAGAACCACAAAGACTTTGCGAGGCTTTGATAAAA
GATTTTGAAGCTAATAAAACTTTTTTTGAAATGCTTGAAATTGAAATTCCAAGTGCTGAAGAATTGCTTGCTGATTTAAA
ACGTTTTAATGAAATTTTAACTCCTTATATTACAGATACTACAAGAATGCTTTGGAAGGCTTTAGATGAAGATAAAAGAG
TACTTTTAGAAGGAGCTCAGGGCTCTATGCTTGATATTGATCATGGAACTTATCCTTATGTTACAAGTTCAAGTACTATT
TCAGCAGGCGCTTTAACAGGACTTGGGTTAAATCCTAAAGAAGCGGGAAATATTATAGGTATTGTAAAAGCTTATGCAAC
GCGTGTGGGAAATGGAGCTTTTCCTACTGAGGATAAGGGCGAAGATGGAGAAAAAATTGCGCAAATTGGAAAAGAGATTG
GCGTAAGCACGGGACGCAAAAGACGCTGTGGATGGTTTGATGCGGTTGCTGTAAGATATACAGCAAGGCTTAATGGTCTT
GATGCTTTATCTTTGATGAAGCTTGATGTTTTAGATGGTTTTGAAAAGATAAAAATTTGTCGTGCTTATGAATATAAGGG
TATGGAAATCGACTATATACCAAGTGATTTAGAAAATGTTCAACCTATTTATGAAGAAATGGATGGTTGGGATAAAGTTT
TTGGTATAAAAGATTATGATTTATTGCCAGAAAATGCTAAAAAATATATAGCAAGACTTGAAGAGCTTGCAGGGGTTAAG
GTAAAGTATATTTCTACTAGCCCTGAAAGAGATGATACTATCATCTTATGA

Upstream 100 bases:

>100_bases
CCACTGACTTCAAATCAGATGAAAGGATAGTTGACTATTCTTTGGGGAGTTCGATTCTCTCATCTTCTCGCCAATATTAA
GTAAAATCAAGGAAATTTTT

Downstream 100 bases:

>100_bases
AAAGTAAATATAATTCTGTTGTAAAGGTAAGAAAACAACAACTTGATAAAGCAGAATCAAATCTTAATCAAGCCAAACAA
AGACAGCTTGAACATGAAAA

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MSKADIIVGIQWGDEGKGKVVDKLCENYDFVCRSAGGHNAGHTIWVNGVRYALHLMPSGVLHPRCINIIGNGVVVSPEVL
IAEMAQFENLKGRLYISDRAHLNLKHHSLIDIAKEKLKGKNAIGTTGKGIGPSYADKINRTGHRVGELLEPQRLCEALIK
DFEANKTFFEMLEIEIPSAEELLADLKRFNEILTPYITDTTRMLWKALDEDKRVLLEGAQGSMLDIDHGTYPYVTSSSTI
SAGALTGLGLNPKEAGNIIGIVKAYATRVGNGAFPTEDKGEDGEKIAQIGKEIGVSTGRKRRCGWFDAVAVRYTARLNGL
DALSLMKLDVLDGFEKIKICRAYEYKGMEIDYIPSDLENVQPIYEEMDGWDKVFGIKDYDLLPENAKKYIARLEELAGVK
VKYISTSPERDDTIIL

Sequences:

>Translated_416_residues
MSKADIIVGIQWGDEGKGKVVDKLCENYDFVCRSAGGHNAGHTIWVNGVRYALHLMPSGVLHPRCINIIGNGVVVSPEVL
IAEMAQFENLKGRLYISDRAHLNLKHHSLIDIAKEKLKGKNAIGTTGKGIGPSYADKINRTGHRVGELLEPQRLCEALIK
DFEANKTFFEMLEIEIPSAEELLADLKRFNEILTPYITDTTRMLWKALDEDKRVLLEGAQGSMLDIDHGTYPYVTSSSTI
SAGALTGLGLNPKEAGNIIGIVKAYATRVGNGAFPTEDKGEDGEKIAQIGKEIGVSTGRKRRCGWFDAVAVRYTARLNGL
DALSLMKLDVLDGFEKIKICRAYEYKGMEIDYIPSDLENVQPIYEEMDGWDKVFGIKDYDLLPENAKKYIARLEELAGVK
VKYISTSPERDDTIIL
>Mature_415_residues
SKADIIVGIQWGDEGKGKVVDKLCENYDFVCRSAGGHNAGHTIWVNGVRYALHLMPSGVLHPRCINIIGNGVVVSPEVLI
AEMAQFENLKGRLYISDRAHLNLKHHSLIDIAKEKLKGKNAIGTTGKGIGPSYADKINRTGHRVGELLEPQRLCEALIKD
FEANKTFFEMLEIEIPSAEELLADLKRFNEILTPYITDTTRMLWKALDEDKRVLLEGAQGSMLDIDHGTYPYVTSSSTIS
AGALTGLGLNPKEAGNIIGIVKAYATRVGNGAFPTEDKGEDGEKIAQIGKEIGVSTGRKRRCGWFDAVAVRYTARLNGLD
ALSLMKLDVLDGFEKIKICRAYEYKGMEIDYIPSDLENVQPIYEEMDGWDKVFGIKDYDLLPENAKKYIARLEELAGVKV
KYISTSPERDDTIIL

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=430, Percent_Identity=42.093023255814, Blast_Score=325, Evalue=6e-89,
Organism=Homo sapiens, GI34577063, Length=441, Percent_Identity=41.4965986394558, Blast_Score=319, Evalue=3e-87,
Organism=Homo sapiens, GI40316944, Length=396, Percent_Identity=41.4141414141414, Blast_Score=288, Evalue=9e-78,
Organism=Escherichia coli, GI1790620, Length=426, Percent_Identity=46.7136150234742, Blast_Score=378, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI25146555, Length=426, Percent_Identity=42.018779342723, Blast_Score=336, Evalue=1e-92,
Organism=Caenorhabditis elegans, GI25146553, Length=426, Percent_Identity=42.018779342723, Blast_Score=336, Evalue=1e-92,
Organism=Saccharomyces cerevisiae, GI6324109, Length=430, Percent_Identity=43.7209302325581, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI21356033, Length=424, Percent_Identity=43.8679245283019, Blast_Score=346, Evalue=2e-95,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 46094; Mature: 45963

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKADIIVGIQWGDEGKGKVVDKLCENYDFVCRSAGGHNAGHTIWVNGVRYALHLMPSGV
CCCCCEEEEEEECCCCCCHHHHHHHCCCCEEEECCCCCCCCCEEEECHHHHHHHHCCCCC
LHPRCINIIGNGVVVSPEVLIAEMAQFENLKGRLYISDRAHLNLKHHSLIDIAKEKLKGK
CCHHHHHHCCCCEEECHHHHHHHHHHHHCCCCEEEEECCCEEEECHHHHHHHHHHHHCCC
NAIGTTGKGIGPSYADKINRTGHRVGELLEPQRLCEALIKDFEANKTFFEMLEIEIPSAE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHEEHHHHCCCCCHH
ELLADLKRFNEILTPYITDTTRMLWKALDEDKRVLLEGAQGSMLDIDHGTYPYVTSSSTI
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCCCEEECCCCC
SAGALTGLGLNPKEAGNIIGIVKAYATRVGNGAFPTEDKGEDGEKIAQIGKEIGVSTGRK
CCCCEECCCCCHHHHCCEEHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC
RRCGWFDAVAVRYTARLNGLDALSLMKLDVLDGFEKIKICRAYEYKGMEIDYIPSDLENV
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEECCCCHHHH
QPIYEEMDGWDKVFGIKDYDLLPENAKKYIARLEELAGVKVKYISTSPERDDTIIL
HHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEC
>Mature Secondary Structure 
SKADIIVGIQWGDEGKGKVVDKLCENYDFVCRSAGGHNAGHTIWVNGVRYALHLMPSGV
CCCCEEEEEEECCCCCCHHHHHHHCCCCEEEECCCCCCCCCEEEECHHHHHHHHCCCCC
LHPRCINIIGNGVVVSPEVLIAEMAQFENLKGRLYISDRAHLNLKHHSLIDIAKEKLKGK
CCHHHHHHCCCCEEECHHHHHHHHHHHHCCCCEEEEECCCEEEECHHHHHHHHHHHHCCC
NAIGTTGKGIGPSYADKINRTGHRVGELLEPQRLCEALIKDFEANKTFFEMLEIEIPSAE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHEEHHHHCCCCCHH
ELLADLKRFNEILTPYITDTTRMLWKALDEDKRVLLEGAQGSMLDIDHGTYPYVTSSSTI
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCCCEEECCCCC
SAGALTGLGLNPKEAGNIIGIVKAYATRVGNGAFPTEDKGEDGEKIAQIGKEIGVSTGRK
CCCCEECCCCCHHHHCCEEHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC
RRCGWFDAVAVRYTARLNGLDALSLMKLDVLDGFEKIKICRAYEYKGMEIDYIPSDLENV
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEECCCCHHHH
QPIYEEMDGWDKVFGIKDYDLLPENAKKYIARLEELAGVKVKYISTSPERDDTIIL
HHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA