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The map label for this gene is thiL [H]

Identifier: 57238482

GI number: 57238482

Start: 1533942

End: 1534763

Strand: Reverse

Name: thiL [H]

Synonym: CJE1632

Alternate gene names: 57238482

Gene position: 1534763-1533942 (Counterclockwise)

Preceding gene: 57238493

Following gene: 57238481

Centisome position: 86.33

GC content: 29.56

Gene sequence:

>822_bases
ATGAACAAAGAAGATTTTATTATCAAAGCTTTTTTAAATGAAAAAAATGGCGATGATGGAGCTATTATTGATGATTGGTG
TTTTAGTAAGGATTTGTTTTTTGAAAATGTGCATTTTAAAAGAGAGTGGTTTAGCTTAGAGCAAATTGCTACAAAAGCAA
TGCTTGTTAATATCTCAGATGCTATTTCAATGAATGCTGTACCAAAATACGCACTTTTAGGACTTGCTTTACCAAAAAAT
TTAAGCGAAAATGAGATCAAAGCCTTGCAAAAAGGTTTTTTAAAAACAGCTAGGAAATTTAATATAAAAATCATAGGCGG
TGATACTATAAGCAATGATAAAATAGACATTAGTCTTACTATCATTTCAAAGATTAACGATAAAGCTGTTTTTAGAAAAG
GTTTAAAAAAGGGGCATTTACTTGCTTATACGGGAAAATTAGGACGAAGTTTAAAAGGGCTTGAAATTTTACAAAATGGG
GGCACTTTAAAACCAAACCATGTATTTATAAAGCCAAAATTAAGAGCTAGTTTTTTTTATGAAATCGCACCTTTAATTTC
TTGTGCTATGGATATTTCAGATGGTTTAAGCAAGGATTTATCAAGACTTTTAGCCTTAAATAAATGCGGTATTTCTTGGT
TTAAAAAACTTGATGATTATACGCTTTATAGCGGAGAAGAATATGAAATTTTGTTTGCTTTTGATGAAAAAGAGCGTCAA
AATATAAAAACAATAGCCAAAAAACACGGCGTGAAATTAAATATCTTTGGAAAAGCAGTGAAAGGAAAATATGAATTTAG
AGGAAGAGAACACCATTTTTAA

Upstream 100 bases:

>100_bases
TCTAATTTTTTCATGGTTTTTCCTTAAATATTTTTATATTATATAAAATTATTCTAAAATTCACTAGAATTTAAAAGGAA
ATTTTATAAAATCACGCATT

Downstream 100 bases:

>100_bases
GCCTTTGTATAGTTTAAAACACAGTCCTATTAATGCGTATTTTAGTAAAAATAGCGATGATTTTGTAGTGCGTGAAAGGC
CTTTGTATGAATTTAGCGGT

Product: thiamine monophosphate kinase

Products: NA

Alternate protein names: Thiamine-phosphate kinase [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MNKEDFIIKAFLNEKNGDDGAIIDDWCFSKDLFFENVHFKREWFSLEQIATKAMLVNISDAISMNAVPKYALLGLALPKN
LSENEIKALQKGFLKTARKFNIKIIGGDTISNDKIDISLTIISKINDKAVFRKGLKKGHLLAYTGKLGRSLKGLEILQNG
GTLKPNHVFIKPKLRASFFYEIAPLISCAMDISDGLSKDLSRLLALNKCGISWFKKLDDYTLYSGEEYEILFAFDEKERQ
NIKTIAKKHGVKLNIFGKAVKGKYEFRGREHHF

Sequences:

>Translated_273_residues
MNKEDFIIKAFLNEKNGDDGAIIDDWCFSKDLFFENVHFKREWFSLEQIATKAMLVNISDAISMNAVPKYALLGLALPKN
LSENEIKALQKGFLKTARKFNIKIIGGDTISNDKIDISLTIISKINDKAVFRKGLKKGHLLAYTGKLGRSLKGLEILQNG
GTLKPNHVFIKPKLRASFFYEIAPLISCAMDISDGLSKDLSRLLALNKCGISWFKKLDDYTLYSGEEYEILFAFDEKERQ
NIKTIAKKHGVKLNIFGKAVKGKYEFRGREHHF
>Mature_273_residues
MNKEDFIIKAFLNEKNGDDGAIIDDWCFSKDLFFENVHFKREWFSLEQIATKAMLVNISDAISMNAVPKYALLGLALPKN
LSENEIKALQKGFLKTARKFNIKIIGGDTISNDKIDISLTIISKINDKAVFRKGLKKGHLLAYTGKLGRSLKGLEILQNG
GTLKPNHVFIKPKLRASFFYEIAPLISCAMDISDGLSKDLSRLLALNKCGISWFKKLDDYTLYSGEEYEILFAFDEKERQ
NIKTIAKKHGVKLNIFGKAVKGKYEFRGREHHF

Specific function: Thiamine biosynthesis. [C]

COG id: COG0611

COG function: function code H; Thiamine monophosphate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine-monophosphate kinase family [H]

Homologues:

Organism=Escherichia coli, GI1786619, Length=271, Percent_Identity=27.3062730627306, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000728
- InterPro:   IPR010918
- InterPro:   IPR016188
- InterPro:   IPR006283 [H]

Pfam domain/function: PF00586 AIRS; PF02769 AIRS_C [H]

EC number: =2.7.4.16 [H]

Molecular weight: Translated: 30989; Mature: 30989

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKEDFIIKAFLNEKNGDDGAIIDDWCFSKDLFFENVHFKREWFSLEQIATKAMLVNISD
CCCCCEEEEEEECCCCCCCCCEEEHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHEEECHH
AISMNAVPKYALLGLALPKNLSENEIKALQKGFLKTARKFNIKIIGGDTISNDKIDISLT
HHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCEEEEEEE
IISKINDKAVFRKGLKKGHLLAYTGKLGRSLKGLEILQNGGTLKPNHVFIKPKLRASFFY
EEECCCHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHCCCCCCCCEEEEECCHHHHHHH
EIAPLISCAMDISDGLSKDLSRLLALNKCGISWFKKLDDYTLYSGEEYEILFAFDEKERQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCEEECCCCEEEEEEECCHHHH
NIKTIAKKHGVKLNIFGKAVKGKYEFRGREHHF
HHHHHHHHCCCEEEEECCEECCEEEECCCCCCC
>Mature Secondary Structure
MNKEDFIIKAFLNEKNGDDGAIIDDWCFSKDLFFENVHFKREWFSLEQIATKAMLVNISD
CCCCCEEEEEEECCCCCCCCCEEEHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHEEECHH
AISMNAVPKYALLGLALPKNLSENEIKALQKGFLKTARKFNIKIIGGDTISNDKIDISLT
HHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCEEEEEEE
IISKINDKAVFRKGLKKGHLLAYTGKLGRSLKGLEILQNGGTLKPNHVFIKPKLRASFFY
EEECCCHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHCCCCCCCCEEEEECCHHHHHHH
EIAPLISCAMDISDGLSKDLSRLLALNKCGISWFKKLDDYTLYSGEEYEILFAFDEKERQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCEEECCCCEEEEEEECCHHHH
NIKTIAKKHGVKLNIFGKAVKGKYEFRGREHHF
HHHHHHHHCCCEEEEECCEECCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377; 8419296 [H]