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The map label for this gene is truD [H]

Identifier: 57238481

GI number: 57238481

Start: 1532855

End: 1533973

Strand: Reverse

Name: truD [H]

Synonym: CJE1631

Alternate gene names: 57238481

Gene position: 1533973-1532855 (Counterclockwise)

Preceding gene: 57238482

Following gene: 57238479

Centisome position: 86.28

GC content: 30.83

Gene sequence:

>1119_bases
ATGAATTTAGAGGAAGAGAACACCATTTTTAAGCCTTTGTATAGTTTAAAACACAGTCCTATTAATGCGTATTTTAGTAA
AAATAGCGATGATTTTGTAGTGCGTGAAAGGCCTTTGTATGAATTTAGCGGTAAGGGTGAACATCTTATTTTGCATATCA
ATAAAAAAGATTTAACCACAAATGAAGCTTTAAAAATTTTAAGCGAAACAAGCGGGGTTAAAATAAGAGATTTTGGTTAT
GCAGGGTTTAAAGACAAGCAAGGATCAACCTTTCAATACCTTTCTATGCCTAAAAAATTTGAGAGTTTTTTGTCAAATTT
TTCTCATCCTAAACTTAAAATTTTAGAAACTTTTATCCATGAAAACAAGCTAAGAATAGGGCATTTAAAAGGAAATACTT
TTTTCATACGCTTAAAAAAAGTTTTACCAAGCGATGCTTTAAAACTAGAACAAGCTTTGATGAATTTGGATAAGCAAGGT
TTTACAAATTATTTTGGTTATCAGCGTTTTGGAAAATTTGGGGATAATTATAAAGAAGGGCTTGAAATTTTGCGTGGCAA
AAAAATGAAAAATGTAAAAATGAAAGAGTTTTTAATTTCAGCTTTTCAAAGTGAGCTTTTTAATCGCTATTTGAGTAAAA
GAGTGGAGTTATCACATTTTGCAAATGATTTTAGCGAAAAAGAATTGATCCAAATTTATAAAATTTCTAAAGAAGAAGCT
AAGGAACTTAAAAAACAAGAACAATTTTTTAAGCTTTTAAAAGGTGAGGTTTTGGGGCATTATCCTTTTGGAAAGTGTTT
TTTATGCGAGGATTTAAGTGCTGAGCTTGGGCGTTTTAAGGCAAGAGATATAAGCGCCATGGGGCTTTTAATCGGTGCTA
AGGCTTATGAAACAGGAGAAGGTTTGGCCTTAAATTTAGAAAATGAAATTTTTAAAGATACTTTGGAGTTTAAGGCTAAA
ATGCAAGGTTCAAGACGCTTTATGTGGGGATATTTAGAAGAGTTAAAATGGCGTTATGATGAAGAAAAAGCTCATTTTTG
TATAGAGTTTTTCTTGCAAAAAGGCTCTTATGCTACTGTGGTTTTAGAAGAAATTTTGCATAAAAATTTGTTTGAGTGA

Upstream 100 bases:

>100_bases
TTGTTTGCTTTTGATGAAAAAGAGCGTCAAAATATAAAAACAATAGCCAAAAAACACGGCGTGAAATTAAATATCTTTGG
AAAAGCAGTGAAAGGAAAAT

Downstream 100 bases:

>100_bases
AATAATTATGAGTTAAATTTTATGTGATTTTTACTTTAGAATTTATTGCAAAAATGATATTTAAATTTTAAATTTAGGTA
TAGTTATATTAATTTAATTT

Product: tRNA pseudouridine synthase D

Products: NA

Alternate protein names: tRNA pseudouridylate synthase D; tRNA-uridine isomerase D [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MNLEEENTIFKPLYSLKHSPINAYFSKNSDDFVVRERPLYEFSGKGEHLILHINKKDLTTNEALKILSETSGVKIRDFGY
AGFKDKQGSTFQYLSMPKKFESFLSNFSHPKLKILETFIHENKLRIGHLKGNTFFIRLKKVLPSDALKLEQALMNLDKQG
FTNYFGYQRFGKFGDNYKEGLEILRGKKMKNVKMKEFLISAFQSELFNRYLSKRVELSHFANDFSEKELIQIYKISKEEA
KELKKQEQFFKLLKGEVLGHYPFGKCFLCEDLSAELGRFKARDISAMGLLIGAKAYETGEGLALNLENEIFKDTLEFKAK
MQGSRRFMWGYLEELKWRYDEEKAHFCIEFFLQKGSYATVVLEEILHKNLFE

Sequences:

>Translated_372_residues
MNLEEENTIFKPLYSLKHSPINAYFSKNSDDFVVRERPLYEFSGKGEHLILHINKKDLTTNEALKILSETSGVKIRDFGY
AGFKDKQGSTFQYLSMPKKFESFLSNFSHPKLKILETFIHENKLRIGHLKGNTFFIRLKKVLPSDALKLEQALMNLDKQG
FTNYFGYQRFGKFGDNYKEGLEILRGKKMKNVKMKEFLISAFQSELFNRYLSKRVELSHFANDFSEKELIQIYKISKEEA
KELKKQEQFFKLLKGEVLGHYPFGKCFLCEDLSAELGRFKARDISAMGLLIGAKAYETGEGLALNLENEIFKDTLEFKAK
MQGSRRFMWGYLEELKWRYDEEKAHFCIEFFLQKGSYATVVLEEILHKNLFE
>Mature_372_residues
MNLEEENTIFKPLYSLKHSPINAYFSKNSDDFVVRERPLYEFSGKGEHLILHINKKDLTTNEALKILSETSGVKIRDFGY
AGFKDKQGSTFQYLSMPKKFESFLSNFSHPKLKILETFIHENKLRIGHLKGNTFFIRLKKVLPSDALKLEQALMNLDKQG
FTNYFGYQRFGKFGDNYKEGLEILRGKKMKNVKMKEFLISAFQSELFNRYLSKRVELSHFANDFSEKELIQIYKISKEEA
KELKKQEQFFKLLKGEVLGHYPFGKCFLCEDLSAELGRFKARDISAMGLLIGAKAYETGEGLALNLENEIFKDTLEFKAK
MQGSRRFMWGYLEELKWRYDEEKAHFCIEFFLQKGSYATVVLEEILHKNLFE

Specific function: Responsible for synthesis of pseudouridine from uracil- 13 in transfer RNAs [H]

COG id: COG0585

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRUD domain [H]

Homologues:

Organism=Homo sapiens, GI148762985, Length=186, Percent_Identity=32.258064516129, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI148762961, Length=186, Percent_Identity=32.258064516129, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI148762948, Length=186, Percent_Identity=32.258064516129, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI50727002, Length=145, Percent_Identity=33.1034482758621, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1789102, Length=351, Percent_Identity=27.9202279202279, Blast_Score=119, Evalue=4e-28,
Organism=Drosophila melanogaster, GI21355799, Length=148, Percent_Identity=29.7297297297297, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001656
- InterPro:   IPR020119
- InterPro:   IPR011760 [H]

Pfam domain/function: PF01142 TruD [H]

EC number: NA

Molecular weight: Translated: 43554; Mature: 43554

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: PS50984 TRUD ; PS01268 UPF0024

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLEEENTIFKPLYSLKHSPINAYFSKNSDDFVVRERPLYEFSGKGEHLILHINKKDLTT
CCCCCCCHHHHHHHHHCCCCCCCEECCCCCCEEEECCCCCEECCCCCEEEEEECCCCCCH
NEALKILSETSGVKIRDFGYAGFKDKQGSTFQYLSMPKKFESFLSNFSHPKLKILETFIH
HHHHHHHHHCCCCEEEECCCCCCCCCCCCEEHHHCCCHHHHHHHHCCCCCHHHHHHHHHH
ENKLRIGHLKGNTFFIRLKKVLPSDALKLEQALMNLDKQGFTNYFGYQRFGKFGDNYKEG
CCCEEEEEECCCEEEEEEHHHCCHHHHHHHHHHHCCCHHCCHHHHHHHHHHHCCCCHHHH
LEILRGKKMKNVKMKEFLISAFQSELFNRYLSKRVELSHFANDFSEKELIQIYKISKEEA
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHH
KELKKQEQFFKLLKGEVLGHYPFGKCFLCEDLSAELGRFKARDISAMGLLIGAKAYETGE
HHHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHCCCHHHHHHHHHHHHCCCCEECCC
GLALNLENEIFKDTLEFKAKMQGSRRFMWGYLEELKWRYDEEKAHFCIEFFLQKGSYATV
CEEEECHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHH
VLEEILHKNLFE
HHHHHHHHHCCC
>Mature Secondary Structure
MNLEEENTIFKPLYSLKHSPINAYFSKNSDDFVVRERPLYEFSGKGEHLILHINKKDLTT
CCCCCCCHHHHHHHHHCCCCCCCEECCCCCCEEEECCCCCEECCCCCEEEEEECCCCCCH
NEALKILSETSGVKIRDFGYAGFKDKQGSTFQYLSMPKKFESFLSNFSHPKLKILETFIH
HHHHHHHHHCCCCEEEECCCCCCCCCCCCEEHHHCCCHHHHHHHHCCCCCHHHHHHHHHH
ENKLRIGHLKGNTFFIRLKKVLPSDALKLEQALMNLDKQGFTNYFGYQRFGKFGDNYKEG
CCCEEEEEECCCEEEEEEHHHCCHHHHHHHHHHHCCCHHCCHHHHHHHHHHHCCCCHHHH
LEILRGKKMKNVKMKEFLISAFQSELFNRYLSKRVELSHFANDFSEKELIQIYKISKEEA
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHH
KELKKQEQFFKLLKGEVLGHYPFGKCFLCEDLSAELGRFKARDISAMGLLIGAKAYETGE
HHHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHCCCHHHHHHHHHHHHCCCCEECCC
GLALNLENEIFKDTLEFKAKMQGSRRFMWGYLEELKWRYDEEKAHFCIEFFLQKGSYATV
CEEEECHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHH
VLEEILHKNLFE
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA