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The map label for this gene is wcbK [H]

Identifier: 57238461

GI number: 57238461

Start: 1512989

End: 1514020

Strand: Reverse

Name: wcbK [H]

Synonym: CJE1611

Alternate gene names: 57238461

Gene position: 1514020-1512989 (Counterclockwise)

Preceding gene: 57238462

Following gene: 57238460

Centisome position: 85.16

GC content: 34.01

Gene sequence:

>1032_bases
ATGAAAAAAACAGCGTTAATTACAGGATTTACAGGGCAAGTTGGTTCTCAAATGGCAGATTTTTTGCTTGCAAATACGGA
TTATGAAGTTATAGGAATGATGCGTTGGCAAGAGCCTATGGACAATATCTATCATTTAAGTGATAGGATTAATAAAAAAG
ATAGAATAAGCATTTTTTATGCAGATTTAAATGATTACTCTAGTCTTCAAAAACTTTTTGAGAGTCAAAGACCTGATGTG
ATTTTTCACCTAGCAGCTCAGTCTTATCCAAAAACTTCTTTTGATATACCGATTGAAACTTTACAGACTAATATAATAGG
TACGGCAAATATCCTAGAAAATATTAGAATTTTAAAGGCGAAAGATGGTTATGACCCTGTAGTGCATGTATGTTCTTCTA
GTGAAGTTTATGGTAGAGCAAAGGTAGGTATTAAGTTAAACGAAGATACAACTTTTCACGGTGCAAGTCCTTATAGTATA
AGCAAAATCGGCACCGATTATTTAGGAAGATTTTATGGCGAAGCTTATAACATTCGCACTTTTGTTACAAGAATGGGAAC
ACATAGTGGCCCAAGACGTTCTGATGTGTTTTTTGAAAGCACGGTTGCAAAGCAAATTGCTTTGATTGAAACGGGTTATC
AAGAGCCTGTTATCAAGGTTGGAAATCTCTCAAGCGTAAGAACCTTTCAAGATGCCAGAGATGCTATAAGGGCTTATTAT
CTACTTTCATTAGAAAGCCAAAAAGGAAAAGTTCCTTGTGGGGAAGCTTTTAATATAGCAGGAGAAGAGGCTTTTAAACT
CCCAGAGGTTATAGAAATTCTTTTATCTTTTTCTACAAGAAAGGATATAAAAATAGAGCAAGATGAAGAAAGATTGCGTC
CTATTGATGCGGATTATCAAATGTTTGATAACACAAAAATCAAAAGTTTTATTAACTGGAAGCCAGAAATTGCAGCTAGA
AAAATGTTTGAAGATTTGTTAAATCATTGGAGAGATGAGATAAAAAAAGGAAGGATTCCGTTAAATAGATGA

Upstream 100 bases:

>100_bases
GTTGTGATAAAATAAAGCAAATGAATTGGCAAGCTTATACAAAATTAGAAGATGGGATAAGATCAACATTTCATTGGTAT
AAAAGTAACAAAGGATTGAT

Downstream 100 bases:

>100_bases
AAACCATACGCACACAAACTCCTCTTCGCCTTGGTTTAGCAGGTGGAGGGACTGATATAAATTTATATTGTGATAAATAC
ACAGGTTATGTTTTAAATGC

Product: GDP-mannose 4,6-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MKKTALITGFTGQVGSQMADFLLANTDYEVIGMMRWQEPMDNIYHLSDRINKKDRISIFYADLNDYSSLQKLFESQRPDV
IFHLAAQSYPKTSFDIPIETLQTNIIGTANILENIRILKAKDGYDPVVHVCSSSEVYGRAKVGIKLNEDTTFHGASPYSI
SKIGTDYLGRFYGEAYNIRTFVTRMGTHSGPRRSDVFFESTVAKQIALIETGYQEPVIKVGNLSSVRTFQDARDAIRAYY
LLSLESQKGKVPCGEAFNIAGEEAFKLPEVIEILLSFSTRKDIKIEQDEERLRPIDADYQMFDNTKIKSFINWKPEIAAR
KMFEDLLNHWRDEIKKGRIPLNR

Sequences:

>Translated_343_residues
MKKTALITGFTGQVGSQMADFLLANTDYEVIGMMRWQEPMDNIYHLSDRINKKDRISIFYADLNDYSSLQKLFESQRPDV
IFHLAAQSYPKTSFDIPIETLQTNIIGTANILENIRILKAKDGYDPVVHVCSSSEVYGRAKVGIKLNEDTTFHGASPYSI
SKIGTDYLGRFYGEAYNIRTFVTRMGTHSGPRRSDVFFESTVAKQIALIETGYQEPVIKVGNLSSVRTFQDARDAIRAYY
LLSLESQKGKVPCGEAFNIAGEEAFKLPEVIEILLSFSTRKDIKIEQDEERLRPIDADYQMFDNTKIKSFINWKPEIAAR
KMFEDLLNHWRDEIKKGRIPLNR
>Mature_343_residues
MKKTALITGFTGQVGSQMADFLLANTDYEVIGMMRWQEPMDNIYHLSDRINKKDRISIFYADLNDYSSLQKLFESQRPDV
IFHLAAQSYPKTSFDIPIETLQTNIIGTANILENIRILKAKDGYDPVVHVCSSSEVYGRAKVGIKLNEDTTFHGASPYSI
SKIGTDYLGRFYGEAYNIRTFVTRMGTHSGPRRSDVFFESTVAKQIALIETGYQEPVIKVGNLSSVRTFQDARDAIRAYY
LLSLESQKGKVPCGEAFNIAGEEAFKLPEVIEILLSFSTRKDIKIEQDEERLRPIDADYQMFDNTKIKSFINWKPEIAAR
KMFEDLLNHWRDEIKKGRIPLNR

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=349, Percent_Identity=27.7936962750716, Blast_Score=125, Evalue=7e-29,
Organism=Homo sapiens, GI7657641, Length=338, Percent_Identity=23.0769230769231, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1788366, Length=369, Percent_Identity=28.7262872628726, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1788353, Length=365, Percent_Identity=26.8493150684932, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI48994969, Length=352, Percent_Identity=27.2727272727273, Blast_Score=98, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI133901786, Length=342, Percent_Identity=29.2397660818713, Blast_Score=134, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI133901790, Length=342, Percent_Identity=29.2397660818713, Blast_Score=134, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI17539422, Length=342, Percent_Identity=29.2397660818713, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI133901788, Length=342, Percent_Identity=29.2397660818713, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17539424, Length=342, Percent_Identity=29.2397660818713, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17507723, Length=342, Percent_Identity=26.9005847953216, Blast_Score=127, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17568069, Length=328, Percent_Identity=25.9146341463415, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17539532, Length=344, Percent_Identity=24.1279069767442, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24158427, Length=342, Percent_Identity=31.5789473684211, Blast_Score=134, Evalue=8e-32,
Organism=Drosophila melanogaster, GI21356223, Length=343, Percent_Identity=23.6151603498542, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 39230; Mature: 39230

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTALITGFTGQVGSQMADFLLANTDYEVIGMMRWQEPMDNIYHLSDRINKKDRISIFY
CCCCEEEECCCCHHHHHHHHHHHCCCCHHHEEHHHHHHHHHHHHHHHHHCCCCCEEEEEE
ADLNDYSSLQKLFESQRPDVIFHLAAQSYPKTSFDIPIETLQTNIIGTANILENIRILKA
ECCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHCCCCHHHHHHCCEEEEE
KDGYDPVVHVCSSSEVYGRAKVGIKLNEDTTFHGASPYSISKIGTDYLGRFYGEAYNIRT
CCCCCHHEEEECCCCCCEEEEEEEEECCCCEECCCCCCCHHHHCHHHHHHHHCCHHHHHH
FVTRMGTHSGPRRSDVFFESTVAKQIALIETGYQEPVIKVGNLSSVRTFQDARDAIRAYY
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHH
LLSLESQKGKVPCGEAFNIAGEEAFKLPEVIEILLSFSTRKDIKIEQDEERLRPIDADYQ
EEEEECCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHCCCCCCEECCCHHHCCCCCCCHH
MFDNTKIKSFINWKPEIAARKMFEDLLNHWRDEIKKGRIPLNR
HHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKKTALITGFTGQVGSQMADFLLANTDYEVIGMMRWQEPMDNIYHLSDRINKKDRISIFY
CCCCEEEECCCCHHHHHHHHHHHCCCCHHHEEHHHHHHHHHHHHHHHHHCCCCCEEEEEE
ADLNDYSSLQKLFESQRPDVIFHLAAQSYPKTSFDIPIETLQTNIIGTANILENIRILKA
ECCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHCCCCHHHHHHCCEEEEE
KDGYDPVVHVCSSSEVYGRAKVGIKLNEDTTFHGASPYSISKIGTDYLGRFYGEAYNIRT
CCCCCHHEEEECCCCCCEEEEEEEEECCCCEECCCCCCCHHHHCHHHHHHHHCCHHHHHH
FVTRMGTHSGPRRSDVFFESTVAKQIALIETGYQEPVIKVGNLSSVRTFQDARDAIRAYY
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHH
LLSLESQKGKVPCGEAFNIAGEEAFKLPEVIEILLSFSTRKDIKIEQDEERLRPIDADYQ
EEEEECCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHCCCCCCEECCCHHHCCCCCCCHH
MFDNTKIKSFINWKPEIAARKMFEDLLNHWRDEIKKGRIPLNR
HHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]