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The map label for this gene is hycF [C]

Identifier: 57238420

GI number: 57238420

Start: 1469116

End: 1470789

Strand: Reverse

Name: hycF [C]

Synonym: CJE1568

Alternate gene names: 57238420

Gene position: 1470789-1469116 (Counterclockwise)

Preceding gene: 57238425

Following gene: 57238418

Centisome position: 82.73

GC content: 31.3

Gene sequence:

>1674_bases
ATGCAAGATTTTGTTTATATAAAAAATGATGTTTTAATCCCTTTGCCTGATGCTATTGAAATTTTAGATCAGGCAAATGA
TAAAGAAACATTAATTTGCAATGATAAAAATCAAAAAGCACAAATTTATGCCCCTGAGATTAATTTTTATCTTAAAAATT
CTCAAGATGAAATTTTAGAGCAAAGTAAAAATGTTTTAACGCTTTATGAAGCAAGGGCTAGCGTTTATGATTTAGGGCTT
GATTTAGAACAAAGCAAAGAAGTGCAAAATCGTTTGATTTTAGTAGATAGCGATACTCAAACTGTAGAGTTTTTAAAAGA
ACATGGATTTAAGGTTATTGCTTTAAGTAGTGCAGAAATTTTAGCTGTTTTTGGAAGTGTGGGGGAGCTTTGTGCTGTGG
TTAAAAATCAAGGCGAAGAAGTGGAAATAGATTTTGATTTTTTACTTTTTAAAGCTGAAAATTTAAGCGTTGTGCGTAAA
GATTTTACAAGACAAAGTGGATGTTATAATCTTTTAAATTTTGAAAATCTTGAAGCATTGTTAGAGTTTTTGCAAAGCAA
AAGTCCAAAATATCCTTATAAAACTTATATTAGCTATAATGCTAGCGTGTGTCAATACCATGAAAGACGCAGTGAACATT
GTGCAAAATGTGCTGAAATTTGTCCTACTGTAGCGATTTTAAAAGATGATGAAAACAAACATTTGGAATTTTCTCAAGTG
GATTGTTTGGGTTGTGGAGGATGTATTAGTGTGTGTCCTAGCGGATCGCTTGATTATGCTCCTATGCCAAGAGAAAGTTT
TTTTACCCTTTGTGAGTTTTATAAAGATAAAAAAATTTTAATTATTCCTAAAAAAATGTCTTTAGAAAATTTAAACCTTG
CTTTACCAAAAGATGTTTTACCCTTTATGATAGAGGGTGAAAAATGGCTTTCTTCTATGCATTTTTTAACACTTTTGCAA
AATAGTGGTGCAAATTTGGTTTTTTATACGGACTTTGTATCTCGTGGTAGCAATGAAGCCATTACGCTTTTAAATACTTT
TTTTGAGAGAAAATTTCAAAAAAAAGCTATTTTTGTTGCTAAAGATGAAAAAGAATTGCAAAATGTTTTAAAAGAGCAAG
AATTTATACAAGATTTAAAATATGATTTTCATAACAACACCTTAACTACAAGAGAAAATTTTGCCAAAAGAATGCAAGAA
ATGATAAAAAATGAAGATTTTGGCAGTATAGAAAGTGGCGAATGGTTGCGTTATGGCAAGGTAGAAATCAATCCTAACAC
CTGTACGCTTTGTCTTTCTTGTGTGGGTGCCTGTAATGTAGGAGCTTTAATCGCTGATGCTAAAGAAAATGCTTTGAAAT
TCAATGCTTCTCTTTGTACGACTTGTGGGTATTGTGAGTTAAGTTGTGCCGAAAAAGATACTTTAAAATTATTTCGTAGT
GGAATGGAGTTTAGAGCTTCTTATTTTGAATATCAAACCATGGCAAAAGATGAGCTTTTTGCCTGTATAGAATGTGGTAA
AGAATTTGCCACCAAAAAAGCTGTAGAAAAAATAGCCAATTTAATGAAGCCTAAATTTGGTAATGATGAGAATAAAATCA
AAACACTTTATTGTTGTGCAGATTGCAAGGCAAAAGTGATGATAAAAGCAATGGGTTTTGCGGGGCAAAATTAG

Upstream 100 bases:

>100_bases
GGAAAAGTTCTAAATTTGTTCATCTTAAGCCTTAAGAAAATATTTGGTTATTTTAACATAGAATTTATAAAATGATGTTT
TAAAGAGATTAAGGAAGACA

Downstream 100 bases:

>100_bases
ATCTTAATTTTGCCTTCTAATTCTTCTTTATTTGTAAGTTTGCTATCAAATTTAACAACAAGATTTTTAGAGTTTTTATA
AATATCTACAACCCCTAAAT

Product: iron-sulfur cluster-binding domain-containing protein

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: Polyferredoxins

Number of amino acids: Translated: 557; Mature: 557

Protein sequence:

>557_residues
MQDFVYIKNDVLIPLPDAIEILDQANDKETLICNDKNQKAQIYAPEINFYLKNSQDEILEQSKNVLTLYEARASVYDLGL
DLEQSKEVQNRLILVDSDTQTVEFLKEHGFKVIALSSAEILAVFGSVGELCAVVKNQGEEVEIDFDFLLFKAENLSVVRK
DFTRQSGCYNLLNFENLEALLEFLQSKSPKYPYKTYISYNASVCQYHERRSEHCAKCAEICPTVAILKDDENKHLEFSQV
DCLGCGGCISVCPSGSLDYAPMPRESFFTLCEFYKDKKILIIPKKMSLENLNLALPKDVLPFMIEGEKWLSSMHFLTLLQ
NSGANLVFYTDFVSRGSNEAITLLNTFFERKFQKKAIFVAKDEKELQNVLKEQEFIQDLKYDFHNNTLTTRENFAKRMQE
MIKNEDFGSIESGEWLRYGKVEINPNTCTLCLSCVGACNVGALIADAKENALKFNASLCTTCGYCELSCAEKDTLKLFRS
GMEFRASYFEYQTMAKDELFACIECGKEFATKKAVEKIANLMKPKFGNDENKIKTLYCCADCKAKVMIKAMGFAGQN

Sequences:

>Translated_557_residues
MQDFVYIKNDVLIPLPDAIEILDQANDKETLICNDKNQKAQIYAPEINFYLKNSQDEILEQSKNVLTLYEARASVYDLGL
DLEQSKEVQNRLILVDSDTQTVEFLKEHGFKVIALSSAEILAVFGSVGELCAVVKNQGEEVEIDFDFLLFKAENLSVVRK
DFTRQSGCYNLLNFENLEALLEFLQSKSPKYPYKTYISYNASVCQYHERRSEHCAKCAEICPTVAILKDDENKHLEFSQV
DCLGCGGCISVCPSGSLDYAPMPRESFFTLCEFYKDKKILIIPKKMSLENLNLALPKDVLPFMIEGEKWLSSMHFLTLLQ
NSGANLVFYTDFVSRGSNEAITLLNTFFERKFQKKAIFVAKDEKELQNVLKEQEFIQDLKYDFHNNTLTTRENFAKRMQE
MIKNEDFGSIESGEWLRYGKVEINPNTCTLCLSCVGACNVGALIADAKENALKFNASLCTTCGYCELSCAEKDTLKLFRS
GMEFRASYFEYQTMAKDELFACIECGKEFATKKAVEKIANLMKPKFGNDENKIKTLYCCADCKAKVMIKAMGFAGQN
>Mature_557_residues
MQDFVYIKNDVLIPLPDAIEILDQANDKETLICNDKNQKAQIYAPEINFYLKNSQDEILEQSKNVLTLYEARASVYDLGL
DLEQSKEVQNRLILVDSDTQTVEFLKEHGFKVIALSSAEILAVFGSVGELCAVVKNQGEEVEIDFDFLLFKAENLSVVRK
DFTRQSGCYNLLNFENLEALLEFLQSKSPKYPYKTYISYNASVCQYHERRSEHCAKCAEICPTVAILKDDENKHLEFSQV
DCLGCGGCISVCPSGSLDYAPMPRESFFTLCEFYKDKKILIIPKKMSLENLNLALPKDVLPFMIEGEKWLSSMHFLTLLQ
NSGANLVFYTDFVSRGSNEAITLLNTFFERKFQKKAIFVAKDEKELQNVLKEQEFIQDLKYDFHNNTLTTRENFAKRMQE
MIKNEDFGSIESGEWLRYGKVEINPNTCTLCLSCVGACNVGALIADAKENALKFNASLCTTCGYCELSCAEKDTLKLFRS
GMEFRASYFEYQTMAKDELFACIECGKEFATKKAVEKIANLMKPKFGNDENKIKTLYCCADCKAKVMIKAMGFAGQN

Specific function: Probable Electron Transfer Protein For Hydrogenase 3 [C]

COG id: COG1145

COG function: function code C; Ferredoxin

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 63298; Mature: 63298

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
4.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDFVYIKNDVLIPLPDAIEILDQANDKETLICNDKNQKAQIYAPEINFYLKNSQDEILE
CCCCEEEECCEEEECCHHHHHHHCCCCCCEEEECCCCCCEEEEECCEEEEEECCHHHHHH
QSKNVLTLYEARASVYDLGLDLEQSKEVQNRLILVDSDTQTVEFLKEHGFKVIALSSAEI
HCCCEEEEEHHHHHHHHCCCCCHHHHHHCCCEEEEECCHHHHHHHHHCCCEEEEECCCHH
LAVFGSVGELCAVVKNQGEEVEIDFDFLLFKAENLSVVRKDFTRQSGCYNLLNFENLEAL
HHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEECCHHHHHHHHHHCCCCEECCCHHHHHHH
LEFLQSKSPKYPYKTYISYNASVCQYHERRSEHCAKCAEICPTVAILKDDENKHLEFSQV
HHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEECCC
DCLGCGGCISVCPSGSLDYAPMPRESFFTLCEFYKDKKILIIPKKMSLENLNLALPKDVL
CEECCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHC
PFMIEGEKWLSSMHFLTLLQNSGANLVFYTDFVSRGSNEAITLLNTFFERKFQKKAIFVA
CEEECCHHHHHHHHHHHHHHCCCCCEEEEEHHHHCCCCCHHHHHHHHHHHHHHHCEEEEE
KDEKELQNVLKEQEFIQDLKYDFHNNTLTTRENFAKRMQEMIKNEDFGSIESGEWLRYGK
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEE
VEINPNTCTLCLSCVGACNVGALIADAKENALKFNASLCTTCGYCELSCAEKDTLKLFRS
EEECCCHHHHHHHHHHCCCCCCEEECCCCCCEEECCHHHCCCCCEEEECCCHHHHHHHHC
GMEFRASYFEYQTMAKDELFACIECGKEFATKKAVEKIANLMKPKFGNDENKIKTLYCCA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEC
DCKAKVMIKAMGFAGQN
CCCCEEEEEEECCCCCC
>Mature Secondary Structure
MQDFVYIKNDVLIPLPDAIEILDQANDKETLICNDKNQKAQIYAPEINFYLKNSQDEILE
CCCCEEEECCEEEECCHHHHHHHCCCCCCEEEECCCCCCEEEEECCEEEEEECCHHHHHH
QSKNVLTLYEARASVYDLGLDLEQSKEVQNRLILVDSDTQTVEFLKEHGFKVIALSSAEI
HCCCEEEEEHHHHHHHHCCCCCHHHHHHCCCEEEEECCHHHHHHHHHCCCEEEEECCCHH
LAVFGSVGELCAVVKNQGEEVEIDFDFLLFKAENLSVVRKDFTRQSGCYNLLNFENLEAL
HHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEECCHHHHHHHHHHCCCCEECCCHHHHHHH
LEFLQSKSPKYPYKTYISYNASVCQYHERRSEHCAKCAEICPTVAILKDDENKHLEFSQV
HHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEECCC
DCLGCGGCISVCPSGSLDYAPMPRESFFTLCEFYKDKKILIIPKKMSLENLNLALPKDVL
CEECCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHC
PFMIEGEKWLSSMHFLTLLQNSGANLVFYTDFVSRGSNEAITLLNTFFERKFQKKAIFVA
CEEECCHHHHHHHHHHHHHHCCCCCEEEEEHHHHCCCCCHHHHHHHHHHHHHHHCEEEEE
KDEKELQNVLKEQEFIQDLKYDFHNNTLTTRENFAKRMQEMIKNEDFGSIESGEWLRYGK
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEE
VEINPNTCTLCLSCVGACNVGALIADAKENALKFNASLCTTCGYCELSCAEKDTLKLFRS
EEECCCHHHHHHHHHHCCCCCCEEECCCCCCEEECCHHHCCCCCEEEECCCHHHHHHHHC
GMEFRASYFEYQTMAKDELFACIECGKEFATKKAVEKIANLMKPKFGNDENKIKTLYCCA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEC
DCKAKVMIKAMGFAGQN
CCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA