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The map label for this gene is hisH [H]

Identifier: 57238367

GI number: 57238367

Start: 1401000

End: 1401605

Strand: Reverse

Name: hisH [H]

Synonym: CJE1510

Alternate gene names: 57238367

Gene position: 1401605-1401000 (Counterclockwise)

Preceding gene: 57238368

Following gene: 57238366

Centisome position: 78.84

GC content: 30.53

Gene sequence:

>606_bases
ATGATAGCGCTCATTGATTACAAGGCGGGAAATTTAAACTCTGTTGCTAAGGCTTTTGAAAAAATAGGAGCTATAAATTT
CATAGCTAAAAATCCAAAAGATTTGCAAAAAGCAGATAAATTGCTTTTACCTGGAGTGGGATCTTTTAAAGAAGCGATGA
AAAATTTAAAAGAACTTGGTTTTATAGAAGCTTTAAAAGAGCAAGTTTTAGTACAAAAAAAACCTATTTTAGGCATTTGT
TTAGGAATGCAGCTTTTTTTAGAAAGGGGCTATGAAGGTGGAGTTTGCGAAGGACTTGGTTTTATAGAAGGTGAAGTAGT
CAAATTTGAAGAGGATTTAAATTTAAAAATCCCACATATGGGTTGGAATGAGCTTGAAATTTTAAAACAAGTTCCTTTGT
ATCAAGGCATAGATAATAAGAGTGATTTTTATTTTGTGCATTCTTTTTATGTGAAATGCAAAGATGAGTTTGTAAGTGCT
AAAGCACAATACGGACATAAATTTGTTGCATCTTTACAAAAAGATCATATTTTTGCTACACAATTTCACCCCGAAAAAAG
TCAAAATTTAGGCTTAAAACTTTTAGAAAATTTTATAAGGCTTTAA

Upstream 100 bases:

>100_bases
ATTCTTTTACCAATAAAAAAATTTTTAAACGTGATGAAAATGGTAAATTTATAAGAGATTATGATGGTTCTTTGGTAAGA
AAAGACGAGTGTGTTTTAAA

Downstream 100 bases:

>100_bases
ATGTTAAAAACTAGAATTATCCCTTGTGTTTTGTTAAAAAACGGACAACTTGTTAAAAGTATAGAATTTAAAGATTTTCG
TACTATAGGGCATTTAACTT

Product: imidazole glycerol phosphate synthase subunit HisH

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase glutamine amidotransferase subunit 1; IGP synthase subunit hisH 1; ImGP synthase subunit hisH 1; IGPS subunit hisH 1 [H]

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MIALIDYKAGNLNSVAKAFEKIGAINFIAKNPKDLQKADKLLLPGVGSFKEAMKNLKELGFIEALKEQVLVQKKPILGIC
LGMQLFLERGYEGGVCEGLGFIEGEVVKFEEDLNLKIPHMGWNELEILKQVPLYQGIDNKSDFYFVHSFYVKCKDEFVSA
KAQYGHKFVASLQKDHIFATQFHPEKSQNLGLKLLENFIRL

Sequences:

>Translated_201_residues
MIALIDYKAGNLNSVAKAFEKIGAINFIAKNPKDLQKADKLLLPGVGSFKEAMKNLKELGFIEALKEQVLVQKKPILGIC
LGMQLFLERGYEGGVCEGLGFIEGEVVKFEEDLNLKIPHMGWNELEILKQVPLYQGIDNKSDFYFVHSFYVKCKDEFVSA
KAQYGHKFVASLQKDHIFATQFHPEKSQNLGLKLLENFIRL
>Mature_201_residues
MIALIDYKAGNLNSVAKAFEKIGAINFIAKNPKDLQKADKLLLPGVGSFKEAMKNLKELGFIEALKEQVLVQKKPILGIC
LGMQLFLERGYEGGVCEGLGFIEGEVVKFEEDLNLKIPHMGWNELEILKQVPLYQGIDNKSDFYFVHSFYVKCKDEFVSA
KAQYGHKFVASLQKDHIFATQFHPEKSQNLGLKLLENFIRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=200, Percent_Identity=38.5, Blast_Score=144, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6319725, Length=212, Percent_Identity=35.8490566037736, Blast_Score=128, Evalue=5e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.- [C]

Molecular weight: Translated: 22741; Mature: 22741

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIALIDYKAGNLNSVAKAFEKIGAINFIAKNPKDLQKADKLLLPGVGSFKEAMKNLKELG
CEEEEECCCCCHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
FIEALKEQVLVQKKPILGICLGMQLFLERGYEGGVCEGLGFIEGEVVKFEEDLNLKIPHM
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCEECCCC
GWNELEILKQVPLYQGIDNKSDFYFVHSFYVKCKDEFVSAKAQYGHKFVASLQKDHIFAT
CCHHHHHHHHCHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
QFHPEKSQNLGLKLLENFIRL
EECCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MIALIDYKAGNLNSVAKAFEKIGAINFIAKNPKDLQKADKLLLPGVGSFKEAMKNLKELG
CEEEEECCCCCHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
FIEALKEQVLVQKKPILGICLGMQLFLERGYEGGVCEGLGFIEGEVVKFEEDLNLKIPHM
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCEECCCC
GWNELEILKQVPLYQGIDNKSDFYFVHSFYVKCKDEFVSAKAQYGHKFVASLQKDHIFAT
CCHHHHHHHHCHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
QFHPEKSQNLGLKLLENFIRL
EECCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10688204 [H]