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The map label for this gene is hisF-1 [H]

Identifier: 57238366

GI number: 57238366

Start: 1400253

End: 1400999

Strand: Reverse

Name: hisF-1 [H]

Synonym: CJE1509

Alternate gene names: 57238366

Gene position: 1400999-1400253 (Counterclockwise)

Preceding gene: 57238367

Following gene: 57238362

Centisome position: 78.8

GC content: 31.73

Gene sequence:

>747_bases
ATGTTAAAAACTAGAATTATCCCTTGTGTTTTGTTAAAAAACGGACAACTTGTTAAAAGTATAGAATTTAAAGATTTTCG
TACTATAGGGCATTTAACTTCAACAATGAGAATTTATAATGCACGCAATGTTGATGAGCTTATTATCTTAGATATAGATG
CTTCAAAAAGTGGAGAAATAGATTTTGAAAGTATAGAAGATCTTGCTAAAGAATGTTTTATGCCTTTAACTATGGGTGGC
GGGATAAAAACCCTTGAAGATATACAAAAGATTTTAAATTTAGGTGCGGATAAAATTTCTATCAATTCTAAGGCCTTAGA
AGATATGGATTTTATAAGTAAAGCGGCTAATCGTTTTGGTTCTCAATGTATAGTTTGTTCTATAGATGTTAAAAGAAAAG
GAGGGCAATTTTGTGTTTATGACAGGGGAAATTTACTCGAAAAAAGTCCTTTAGAACTTGCTTTAGAATATGAAAAAAAA
GGAGCTGGAGAGCTTCTTTTAACTTCTGTGGATTTTGAAGGAAGGGCAAAGGGTTATGATTTAGAACTTTTAAAGATCTT
TCAAAATAAACTTAAAATTCCTCTTATTATCAATGGAGGACTTAGCAATCCAAGTGATGGAGTTGAGGCTTTAAATTTAG
GTGCTGATGCTTTAGCGGGTGCTTATATTTTTCATTTTTCTAAGTATACCCCTAAAGATGTAAAAGAAGAATTAGCTAGG
CAAGGCTTTGCAGTCAGATTGCTTTAG

Upstream 100 bases:

>100_bases
TGCATCTTTACAAAAAGATCATATTTTTGCTACACAATTTCACCCCGAAAAAAGTCAAAATTTAGGCTTAAAACTTTTAG
AAAATTTTATAAGGCTTTAA

Downstream 100 bases:

>100_bases
GCAAATATGGTAAAAATCTTTATCTTCATCATAAATAGTAAAATGATTTTGTTGATATAATTTTAAAGCTTTGCGATTGT
CTTTGAAAACATAAGCTTTA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF2; ImGP synthase subunit hisF2; IGPS subunit hisF2 [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MLKTRIIPCVLLKNGQLVKSIEFKDFRTIGHLTSTMRIYNARNVDELIILDIDASKSGEIDFESIEDLAKECFMPLTMGG
GIKTLEDIQKILNLGADKISINSKALEDMDFISKAANRFGSQCIVCSIDVKRKGGQFCVYDRGNLLEKSPLELALEYEKK
GAGELLLTSVDFEGRAKGYDLELLKIFQNKLKIPLIINGGLSNPSDGVEALNLGADALAGAYIFHFSKYTPKDVKEELAR
QGFAVRLL

Sequences:

>Translated_248_residues
MLKTRIIPCVLLKNGQLVKSIEFKDFRTIGHLTSTMRIYNARNVDELIILDIDASKSGEIDFESIEDLAKECFMPLTMGG
GIKTLEDIQKILNLGADKISINSKALEDMDFISKAANRFGSQCIVCSIDVKRKGGQFCVYDRGNLLEKSPLELALEYEKK
GAGELLLTSVDFEGRAKGYDLELLKIFQNKLKIPLIINGGLSNPSDGVEALNLGADALAGAYIFHFSKYTPKDVKEELAR
QGFAVRLL
>Mature_248_residues
MLKTRIIPCVLLKNGQLVKSIEFKDFRTIGHLTSTMRIYNARNVDELIILDIDASKSGEIDFESIEDLAKECFMPLTMGG
GIKTLEDIQKILNLGADKISINSKALEDMDFISKAANRFGSQCIVCSIDVKRKGGQFCVYDRGNLLEKSPLELALEYEKK
GAGELLLTSVDFEGRAKGYDLELLKIFQNKLKIPLIINGGLSNPSDGVEALNLGADALAGAYIFHFSKYTPKDVKEELAR
QGFAVRLL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=36.9649805447471, Blast_Score=160, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=29.9363057324841, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR020021
- InterPro:   IPR006062
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.- [C]

Molecular weight: Translated: 27456; Mature: 27456

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKTRIIPCVLLKNGQLVKSIEFKDFRTIGHLTSTMRIYNARNVDELIILDIDASKSGEI
CCCCCEEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCC
DFESIEDLAKECFMPLTMGGGIKTLEDIQKILNLGADKISINSKALEDMDFISKAANRFG
CHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHCC
SQCIVCSIDVKRKGGQFCVYDRGNLLEKSPLELALEYEKKGAGELLLTSVDFEGRAKGYD
CEEEEEEEEEECCCCCEEEECCCCCCCCCCHHEEEEECCCCCCEEEEEEECCCCCCCCCC
LELLKIFQNKLKIPLIINGGLSNPSDGVEALNLGADALAGAYIFHFSKYTPKDVKEELAR
HHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHH
QGFAVRLL
CCEEEEEC
>Mature Secondary Structure
MLKTRIIPCVLLKNGQLVKSIEFKDFRTIGHLTSTMRIYNARNVDELIILDIDASKSGEI
CCCCCEEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCC
DFESIEDLAKECFMPLTMGGGIKTLEDIQKILNLGADKISINSKALEDMDFISKAANRFG
CHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHCC
SQCIVCSIDVKRKGGQFCVYDRGNLLEKSPLELALEYEKKGAGELLLTSVDFEGRAKGYD
CEEEEEEEEEECCCCCEEEECCCCCCCCCCHHEEEEECCCCCCEEEEEEECCCCCCCCCC
LELLKIFQNKLKIPLIINGGLSNPSDGVEALNLGADALAGAYIFHFSKYTPKDVKEELAR
HHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHH
QGFAVRLL
CCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10688204 [H]