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The map label for this gene is waaM [H]

Identifier: 57238015

GI number: 57238015

Start: 1193562

End: 1194449

Strand: Direct

Name: waaM [H]

Synonym: CJE1276

Alternate gene names: 57238015

Gene position: 1193562-1194449 (Clockwise)

Preceding gene: 57238014

Following gene: 57238016

Centisome position: 67.14

GC content: 31.19

Gene sequence:

>888_bases
ATGAAAAATAGTGATAGAATATATCTTAGTCTTTATTATATTTTGAAATTTTTTGTTACTTTTATGCCTGAGTGTATCTT
GCATTTTTTAGCTTTAATTGTAGCAAGAATCACTTTTTATCTTAACAAAAAACATCGCAAAATCATCGATACAAATTTGC
AAATCTGCTTCCCTCAATACACTCAAAAAGAACGCGATAAATTGTCTTTGAAAATTTATGAAAATTTTGCTCAATTTGGA
ATTGATTGTTTGCAAAATCAAAACACCACCAAAGAAAAAATTCTCAATAAAGTAAATTTCATCAATGAAAATTTACTTAT
AGACGCTCTAGCTTTAAAGCGTCCTATTATCTTCACAACTGCACACTATGGAAACTGGGAAATTTTAAGCCTTGCTTATG
CGGCTAAATATGGTGCGATTGCCATAGTAGGGAAAAAGTTAAAAAGTGAAATTATGTATGAAATTTTAAGCCAAAGTCGC
ACCCAATTTGACATAGAACTTATCGACAAAAAAGGCGGCATAAGACAAATGCTAAGCGCTCTAAAAAAAGGGAGAACTTT
AGGAATTTTAACCGATCAAGACTGCGCAGAAAACGAAAGCGTAAGATTAAAATTTTTTAACAAAGAAGTGAATTATCAAA
TGGGAGCAAGCCTTATCGCACAAAGAAGCAATGCTTTGATCATCCCTGTTTATGCCTATAAAGAAGATGGTAAATTTTGC
ATAGAGTTTTTTAAAGCAAAAGATTCTCAAAATGCAAGCTTAGAAGAACTGACACTTTATCAAGCACAAAGTTGCGAAGA
AATGATTAAAAAAAGACCTTGGGAATACTTTTTTTTTCATAGACGCTTTGCAAGTTATAATAAGGAAATTTACAAGGATG
CAAAATGA

Upstream 100 bases:

>100_bases
GGTAAAAAAATCCAAAATGCCAAGCATATCGATAAAAGTGATTTTTGTATCACGCGTATAGAAGAAGAAGATATCTTCAA
ACTTGCCAAAGGCTTACTTA

Downstream 100 bases:

>100_bases
ATCTAAAGCAAATAAGTGTTATTATCATCGTAAAAAATGCAAAACAAACCTTGCTTGAATGTTTAAATTCTTTAAAAGAT
TTTGATGAAATTATTTTACT

Product: lipid A biosynthesis lauroyl acyltransferase

Products: KDO2-(lauroyl)-lipid IVA [C]

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MKNSDRIYLSLYYILKFFVTFMPECILHFLALIVARITFYLNKKHRKIIDTNLQICFPQYTQKERDKLSLKIYENFAQFG
IDCLQNQNTTKEKILNKVNFINENLLIDALALKRPIIFTTAHYGNWEILSLAYAAKYGAIAIVGKKLKSEIMYEILSQSR
TQFDIELIDKKGGIRQMLSALKKGRTLGILTDQDCAENESVRLKFFNKEVNYQMGASLIAQRSNALIIPVYAYKEDGKFC
IEFFKAKDSQNASLEELTLYQAQSCEEMIKKRPWEYFFFHRRFASYNKEIYKDAK

Sequences:

>Translated_295_residues
MKNSDRIYLSLYYILKFFVTFMPECILHFLALIVARITFYLNKKHRKIIDTNLQICFPQYTQKERDKLSLKIYENFAQFG
IDCLQNQNTTKEKILNKVNFINENLLIDALALKRPIIFTTAHYGNWEILSLAYAAKYGAIAIVGKKLKSEIMYEILSQSR
TQFDIELIDKKGGIRQMLSALKKGRTLGILTDQDCAENESVRLKFFNKEVNYQMGASLIAQRSNALIIPVYAYKEDGKFC
IEFFKAKDSQNASLEELTLYQAQSCEEMIKKRPWEYFFFHRRFASYNKEIYKDAK
>Mature_295_residues
MKNSDRIYLSLYYILKFFVTFMPECILHFLALIVARITFYLNKKHRKIIDTNLQICFPQYTQKERDKLSLKIYENFAQFG
IDCLQNQNTTKEKILNKVNFINENLLIDALALKRPIIFTTAHYGNWEILSLAYAAKYGAIAIVGKKLKSEIMYEILSQSR
TQFDIELIDKKGGIRQMLSALKKGRTLGILTDQDCAENESVRLKFFNKEVNYQMGASLIAQRSNALIIPVYAYKEDGKFC
IEFFKAKDSQNASLEELTLYQAQSCEEMIKKRPWEYFFFHRRFASYNKEIYKDAK

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=281, Percent_Identity=22.7758007117438, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.- [C]

Molecular weight: Translated: 34628; Mature: 34628

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNSDRIYLSLYYILKFFVTFMPECILHFLALIVARITFYLNKKHRKIIDTNLQICFPQY
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCH
TQKERDKLSLKIYENFAQFGIDCLQNQNTTKEKILNKVNFINENLLIDALALKRPIIFTT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEE
AHYGNWEILSLAYAAKYGAIAIVGKKLKSEIMYEILSQSRTQFDIELIDKKGGIRQMLSA
ECCCCCHHHHHHHHHHCCHHEEHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHH
LKKGRTLGILTDQDCAENESVRLKFFNKEVNYQMGASLIAQRSNALIIPVYAYKEDGKFC
HHCCCEEEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEEEEEEECCCHHH
IEFFKAKDSQNASLEELTLYQAQSCEEMIKKRPWEYFFFHRRFASYNKEIYKDAK
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKNSDRIYLSLYYILKFFVTFMPECILHFLALIVARITFYLNKKHRKIIDTNLQICFPQY
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCH
TQKERDKLSLKIYENFAQFGIDCLQNQNTTKEKILNKVNFINENLLIDALALKRPIIFTT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEE
AHYGNWEILSLAYAAKYGAIAIVGKKLKSEIMYEILSQSRTQFDIELIDKKGGIRQMLSA
ECCCCCHHHHHHHHHHCCHHEEHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHH
LKKGRTLGILTDQDCAENESVRLKFFNKEVNYQMGASLIAQRSNALIIPVYAYKEDGKFC
HHCCCEEEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEEEEEEECCCHHH
IEFFKAKDSQNASLEELTLYQAQSCEEMIKKRPWEYFFFHRRFASYNKEIYKDAK
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: KDO2-lipid IVA; lauroyl-ACP [C]

Specific reaction: KDO2-lipid IVA + lauroyl-ACP = KDO2-(lauroyl)-lipid IVA + [acyl carrier protein] [C]

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]