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The map label for this gene is truB [H]

Identifier: 57237984

GI number: 57237984

Start: 1163646

End: 1164464

Strand: Direct

Name: truB [H]

Synonym: CJE1245

Alternate gene names: 57237984

Gene position: 1163646-1164464 (Clockwise)

Preceding gene: 57237983

Following gene: 57237985

Centisome position: 65.45

GC content: 28.33

Gene sequence:

>819_bases
ATGAACAAGATCTTTGCAGCCTTTAAACCAAAGGGTTTGAGTTCTAATGCCTTTTTAAGTACTTTAAAGAAAAAATATAA
AAATAAAAAAGCAGGATATTCTGGCACTCTTGATCCTTTCGCCAAAGGGGTTTTAATCGTTGCATTTGGACAATACACAA
AACTTTTCCGTTTTTTAAAAAAAACCCCAAAAACCTATAAAGCTACTTTATGGCTTGGAGTTTACTCCTTAAGTCTTGAT
GATCAAAATATAAAAGAAATTCAAAATATAAAAGAATTTGATCTTGCAAATTTAAAACAAATCATTGATCAAATGCAAGG
AATTATCTCCTATACACCACCTCAATTTAGCGCCAAAAGAATCAATGGAACTCGCGCCTATGAACTTGCAAAAAAAGGTA
TAGAAGCAAATTTAAAACCTTGTCAAATGGAAGTATTTGATTGTAAGATCTTAAGCTATAATCATCCTTTTTTAAATATT
GAAATCACAGTCAGTGAAGGTGCTTACATAAGATCTTATTGTGAACTTTTTGCTAGAAAACTAGGTATTAATGCGACTTT
AAGCTCTTTAGAGCGCATTAAAGAAGGCAAATTTGTTTATAATAATGAGAAAAGCTTAAATGTGTTAAAATACATTAACC
TAAAGCCAAATTTTATCAAGGATTTAAATAAACTCGAAAATGGTGCTAAAATTTTTGTCGAAGAATTAGAATTTCATGAT
GAAGGAGATTATTATATTGAAACAGAAAAATATTTTAGCATAATTAATATTAAAGAAAATACAGTAAAATATCTTCTAAA
TAAGGTTGAAAAATGTTAA

Upstream 100 bases:

>100_bases
GGGTTGTTGAAGTTAATAAAAGCGGAAAAGAGGAAAAGCTTAATATTAATTTTGGTGGTATAATGCGTGTAATCATGGCA
AGCTTTGTGGAAAAAGCAGT

Downstream 100 bases:

>100_bases
TATTATCAAGAAAAGAAAATGAAAGTATAATTATCGGAGAAGGTATAGAAATCAAAGTCGTTCAAACAGGGAAAGGATAT
GCCAAAATAGGTATAGAAGC

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MNKIFAAFKPKGLSSNAFLSTLKKKYKNKKAGYSGTLDPFAKGVLIVAFGQYTKLFRFLKKTPKTYKATLWLGVYSLSLD
DQNIKEIQNIKEFDLANLKQIIDQMQGIISYTPPQFSAKRINGTRAYELAKKGIEANLKPCQMEVFDCKILSYNHPFLNI
EITVSEGAYIRSYCELFARKLGINATLSSLERIKEGKFVYNNEKSLNVLKYINLKPNFIKDLNKLENGAKIFVEELEFHD
EGDYYIETEKYFSIINIKENTVKYLLNKVEKC

Sequences:

>Translated_272_residues
MNKIFAAFKPKGLSSNAFLSTLKKKYKNKKAGYSGTLDPFAKGVLIVAFGQYTKLFRFLKKTPKTYKATLWLGVYSLSLD
DQNIKEIQNIKEFDLANLKQIIDQMQGIISYTPPQFSAKRINGTRAYELAKKGIEANLKPCQMEVFDCKILSYNHPFLNI
EITVSEGAYIRSYCELFARKLGINATLSSLERIKEGKFVYNNEKSLNVLKYINLKPNFIKDLNKLENGAKIFVEELEFHD
EGDYYIETEKYFSIINIKENTVKYLLNKVEKC
>Mature_272_residues
MNKIFAAFKPKGLSSNAFLSTLKKKYKNKKAGYSGTLDPFAKGVLIVAFGQYTKLFRFLKKTPKTYKATLWLGVYSLSLD
DQNIKEIQNIKEFDLANLKQIIDQMQGIISYTPPQFSAKRINGTRAYELAKKGIEANLKPCQMEVFDCKILSYNHPFLNI
EITVSEGAYIRSYCELFARKLGINATLSSLERIKEGKFVYNNEKSLNVLKYINLKPNFIKDLNKLENGAKIFVEELEFHD
EGDYYIETEKYFSIINIKENTVKYLLNKVEKC

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=257, Percent_Identity=28.0155642023346, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI2367200, Length=211, Percent_Identity=30.3317535545024, Blast_Score=103, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6324037, Length=205, Percent_Identity=28.780487804878, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780 [H]

Pfam domain/function: PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 31338; Mature: 31338

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKIFAAFKPKGLSSNAFLSTLKKKYKNKKAGYSGTLDPFAKGVLIVAFGQYTKLFRFLK
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHCCEEEEEEHHHHHHHHHHH
KTPKTYKATLWLGVYSLSLDDQNIKEIQNIKEFDLANLKQIIDQMQGIISYTPPQFSAKR
HCCCCEEEEEEEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHH
INGTRAYELAKKGIEANLKPCQMEVFDCKILSYNHPFLNIEITVSEGAYIRSYCELFARK
CCCHHHHHHHHHCCCCCCCCCEEEEEEEEEEEECCCEEEEEEEECCCHHHHHHHHHHHHH
LGINATLSSLERIKEGKFVYNNEKSLNVLKYINLKPNFIKDLNKLENGAKIFVEELEFHD
HCCHHHHHHHHHHHCCCEEECCCCCEEEEEEECCCCHHHHHHHHHCCCHHEEHHHHHCCC
EGDYYIETEKYFSIINIKENTVKYLLNKVEKC
CCCEEEEHHHEEEEEEECHHHHHHHHHHHHCC
>Mature Secondary Structure
MNKIFAAFKPKGLSSNAFLSTLKKKYKNKKAGYSGTLDPFAKGVLIVAFGQYTKLFRFLK
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHCCEEEEEEHHHHHHHHHHH
KTPKTYKATLWLGVYSLSLDDQNIKEIQNIKEFDLANLKQIIDQMQGIISYTPPQFSAKR
HCCCCEEEEEEEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHH
INGTRAYELAKKGIEANLKPCQMEVFDCKILSYNHPFLNIEITVSEGAYIRSYCELFARK
CCCHHHHHHHHHCCCCCCCCCEEEEEEEEEEEECCCEEEEEEEECCCHHHHHHHHHHHHH
LGINATLSSLERIKEGKFVYNNEKSLNVLKYINLKPNFIKDLNKLENGAKIFVEELEFHD
HCCHHHHHHHHHHHCCCEEECCCCCEEEEEEECCCCHHHHHHHHHCCCHHEEHHHHHCCC
EGDYYIETEKYFSIINIKENTVKYLLNKVEKC
CCCEEEEHHHEEEEEEECHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA