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The map label for this gene is pcrA [H]

Identifier: 57237983

GI number: 57237983

Start: 1161574

End: 1163649

Strand: Direct

Name: pcrA [H]

Synonym: CJE1244

Alternate gene names: 57237983

Gene position: 1161574-1163649 (Clockwise)

Preceding gene: 57237982

Following gene: 57237984

Centisome position: 65.34

GC content: 29.72

Gene sequence:

>2076_bases
ATGAATCTATTTGAAGATTTAAATGATAGTCAAAAACAAGCGGTAAGCCACATTGATGGAGCCATGCTTATCCTAGCAGG
CGCAGGTAGTGGAAAAACCAAAACTATCACCACTCGTCTTGCTTATCTTATCGGTGAAGTAGGCATCCCCTCACATAACA
CTCTTACTCTTACTTTTACAAATAAAGCAGCTAGTGTTATGCGTCATAGAGCATTAAATTTTCTACAAGGCAATCACAAT
CCTTTGCTTTGCACCTTTCATAAATTTGGACTTTTATTTTTAAAACTCCATTTTGAAAGATTGGAGCGTAAAAATAGTTT
TATTGTTATCGATACAGATGATACAAAAAAAATAATTAAAGACTTAATCCATGATAAAAACAAAGATAATGTCTATGATA
TTATCCAATACATATCTTATTGCAAAAATGAAGGCAAAAGAGTAAGCAATGTCTTTGAAGATTTAAATTTACTAAAAGAA
CATAATTTTGAAAAATATCAAAATGAATACAAATTTGCCAACTATTACCGTGCTTATGAAGAATACTTATTAAAACAAAA
TTTTGTAGATTTTGATGATCTTTTATTGCTAAGCAATCTCATTTTAGAAAATGATATAAATTTTGCCAAAGAACAAAGCT
TACTTTATAATTACATTACAGTTGATGAGTATCAAGATACTAATACCTTGCAATATAAAATTCTAAAAAATCTATGTTGT
ATGCATGAAAATATCACAGTTGTTGGTGATGATGATCAAAGTATTTATAGTTGGCGTGGAGCAAAAATTGAAAATATTTT
AAATTTTCAAAATGATTTTAAAAATGTTAAACTTGCAAAACTTGAACAAAACTATCGTTCAGTAGGGACTATTTTACAAG
CAGCAAATAATCTCATATCTCACAATGAGCAACGACTTGGAAAAACTTTAATCTGCACTAAAGATACTGGGGAAAATATT
AAAATATTAAAAAACGAAAACGAAAAAGATGAAGGTTTATATATCGCTCAAGAAGTGAAAAAATTGCTAAATTCTGGAGT
AGAAGCTAAAGAGATCGCCATTTTATTTCGAGTTAATGCACTATCAAGAGCAATAGAAGAAGCATTTATGAAGAAACAAA
TTTCTTATAAACTACTTAGTGGTATGCGTTTTTATGAGAGATTAGAAATAAAAGATTTAATCTCGTATCTTAGACTTATC
TTAAATCCAAGCGATGATTTGTCGTTTAAACGTATTATTAATCGCCCCAAAAGATCCATAGGAGAAAAAGCCCTAAAAAA
TTTAGAAGAATATGCAAAAAAACGTCAAATTTCACTTTTTGATGCCCTATGCGAAAGCGATGGTGGTGTAGGAATTTTAA
CAACAAAAAAAGCACAAAATGAAGCAAATATTTTTATACAAAATATTCACACTCTAAAAAGCTACGATAATGCAAAAAAG
GTTTTTGACAATATAGAAGAATTATTTAAAATCAAAGATTATTTTTCAGAGCAAGATGATGGAGATGAAAGAATCAGAAA
TCTTGATGAGTTTTATGCTAATCTAAGAGAAAAACTCAAAGAAGATCCCGAAGCTTCTTTAGAAGATCTACTTAGTGAAA
TTTCTTTATTAAGTGATCAAGATAATTTAGATGAAGAATGTGTTTGCTTGATGAGTATTCATGCTAGCAAAGGTTTGGAA
TTTGATTATGTTTTTATAATAGGCTTTGATGAAGGATTTTTTCCTTTAAATAGCGAAGAAAATCTCGAAGAAGAACGCCG
TCTTGCTTATGTTGCCATCACTCGCGCCAAGAAATTTCTCACAATAAGTGTTGCAAATTCTCGTTTTTATCATGGTTCAA
GAGCAAATATCAATCCTAGTCGCTTCTTAGAAGAAAGTAAATTAATCAATGAAAAATCAAAAAATCAAAATATACAAAAA
ACTAGTTTTTGCAAAGGAGATCTAGTTAAACATAAAATTTTTGGCATTGGCAGGGTTGTTGAAGTTAATAAAAGCGGAAA
AGAGGAAAAGCTTAATATTAATTTTGGTGGTATAATGCGTGTAATCATGGCAAGCTTTGTGGAAAAAGCAGTATGA

Upstream 100 bases:

>100_bases
TTGTTAATTTTAGCCTTTATAAACGCGAAGATAGCAAAAATTTACAAAAATCGAAAAATATATTTTTAAATAATCCAAAA
AATAAAGATATTTTCAAAAA

Downstream 100 bases:

>100_bases
ACAAGATCTTTGCAGCCTTTAAACCAAAGGGTTTGAGTTCTAATGCCTTTTTAAGTACTTTAAAGAAAAAATATAAAAAT
AAAAAAGCAGGATATTCTGG

Product: ATP-dependent DNA helicase UvrD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 691; Mature: 691

Protein sequence:

>691_residues
MNLFEDLNDSQKQAVSHIDGAMLILAGAGSGKTKTITTRLAYLIGEVGIPSHNTLTLTFTNKAASVMRHRALNFLQGNHN
PLLCTFHKFGLLFLKLHFERLERKNSFIVIDTDDTKKIIKDLIHDKNKDNVYDIIQYISYCKNEGKRVSNVFEDLNLLKE
HNFEKYQNEYKFANYYRAYEEYLLKQNFVDFDDLLLLSNLILENDINFAKEQSLLYNYITVDEYQDTNTLQYKILKNLCC
MHENITVVGDDDQSIYSWRGAKIENILNFQNDFKNVKLAKLEQNYRSVGTILQAANNLISHNEQRLGKTLICTKDTGENI
KILKNENEKDEGLYIAQEVKKLLNSGVEAKEIAILFRVNALSRAIEEAFMKKQISYKLLSGMRFYERLEIKDLISYLRLI
LNPSDDLSFKRIINRPKRSIGEKALKNLEEYAKKRQISLFDALCESDGGVGILTTKKAQNEANIFIQNIHTLKSYDNAKK
VFDNIEELFKIKDYFSEQDDGDERIRNLDEFYANLREKLKEDPEASLEDLLSEISLLSDQDNLDEECVCLMSIHASKGLE
FDYVFIIGFDEGFFPLNSEENLEEERRLAYVAITRAKKFLTISVANSRFYHGSRANINPSRFLEESKLINEKSKNQNIQK
TSFCKGDLVKHKIFGIGRVVEVNKSGKEEKLNINFGGIMRVIMASFVEKAV

Sequences:

>Translated_691_residues
MNLFEDLNDSQKQAVSHIDGAMLILAGAGSGKTKTITTRLAYLIGEVGIPSHNTLTLTFTNKAASVMRHRALNFLQGNHN
PLLCTFHKFGLLFLKLHFERLERKNSFIVIDTDDTKKIIKDLIHDKNKDNVYDIIQYISYCKNEGKRVSNVFEDLNLLKE
HNFEKYQNEYKFANYYRAYEEYLLKQNFVDFDDLLLLSNLILENDINFAKEQSLLYNYITVDEYQDTNTLQYKILKNLCC
MHENITVVGDDDQSIYSWRGAKIENILNFQNDFKNVKLAKLEQNYRSVGTILQAANNLISHNEQRLGKTLICTKDTGENI
KILKNENEKDEGLYIAQEVKKLLNSGVEAKEIAILFRVNALSRAIEEAFMKKQISYKLLSGMRFYERLEIKDLISYLRLI
LNPSDDLSFKRIINRPKRSIGEKALKNLEEYAKKRQISLFDALCESDGGVGILTTKKAQNEANIFIQNIHTLKSYDNAKK
VFDNIEELFKIKDYFSEQDDGDERIRNLDEFYANLREKLKEDPEASLEDLLSEISLLSDQDNLDEECVCLMSIHASKGLE
FDYVFIIGFDEGFFPLNSEENLEEERRLAYVAITRAKKFLTISVANSRFYHGSRANINPSRFLEESKLINEKSKNQNIQK
TSFCKGDLVKHKIFGIGRVVEVNKSGKEEKLNINFGGIMRVIMASFVEKAV
>Mature_691_residues
MNLFEDLNDSQKQAVSHIDGAMLILAGAGSGKTKTITTRLAYLIGEVGIPSHNTLTLTFTNKAASVMRHRALNFLQGNHN
PLLCTFHKFGLLFLKLHFERLERKNSFIVIDTDDTKKIIKDLIHDKNKDNVYDIIQYISYCKNEGKRVSNVFEDLNLLKE
HNFEKYQNEYKFANYYRAYEEYLLKQNFVDFDDLLLLSNLILENDINFAKEQSLLYNYITVDEYQDTNTLQYKILKNLCC
MHENITVVGDDDQSIYSWRGAKIENILNFQNDFKNVKLAKLEQNYRSVGTILQAANNLISHNEQRLGKTLICTKDTGENI
KILKNENEKDEGLYIAQEVKKLLNSGVEAKEIAILFRVNALSRAIEEAFMKKQISYKLLSGMRFYERLEIKDLISYLRLI
LNPSDDLSFKRIINRPKRSIGEKALKNLEEYAKKRQISLFDALCESDGGVGILTTKKAQNEANIFIQNIHTLKSYDNAKK
VFDNIEELFKIKDYFSEQDDGDERIRNLDEFYANLREKLKEDPEASLEDLLSEISLLSDQDNLDEECVCLMSIHASKGLE
FDYVFIIGFDEGFFPLNSEENLEEERRLAYVAITRAKKFLTISVANSRFYHGSRANINPSRFLEESKLINEKSKNQNIQK
TSFCKGDLVKHKIFGIGRVVEVNKSGKEEKLNINFGGIMRVIMASFVEKAV

Specific function: Essential helicase [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=717, Percent_Identity=34.5885634588563, Blast_Score=401, Evalue=1e-113,
Organism=Escherichia coli, GI48994965, Length=645, Percent_Identity=37.3643410852713, Blast_Score=359, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6322369, Length=463, Percent_Identity=26.7818574514039, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6324477, Length=441, Percent_Identity=23.3560090702948, Blast_Score=70, Evalue=8e-13,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 79790; Mature: 79790

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLFEDLNDSQKQAVSHIDGAMLILAGAGSGKTKTITTRLAYLIGEVGIPSHNTLTLTFT
CCCHHHCCHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEC
NKAASVMRHRALNFLQGNHNPLLCTFHKFGLLFLKLHFERLERKNSFIVIDTDDTKKIIK
CHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHH
DLIHDKNKDNVYDIIQYISYCKNEGKRVSNVFEDLNLLKEHNFEKYQNEYKFANYYRAYE
HHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
EYLLKQNFVDFDDLLLLSNLILENDINFAKEQSLLYNYITVDEYQDTNTLQYKILKNLCC
HHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHH
MHENITVVGDDDQSIYSWRGAKIENILNFQNDFKNVKLAKLEQNYRSVGTILQAANNLIS
HCCCEEEEECCCCHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHC
HNEQRLGKTLICTKDTGENIKILKNENEKDEGLYIAQEVKKLLNSGVEAKEIAILFRVNA
CCHHHCCCEEEEECCCCCCEEEEECCCCCCCCEEHHHHHHHHHHCCCCHHHEEEEEEHHH
LSRAIEEAFMKKQISYKLLSGMRFYERLEIKDLISYLRLILNPSDDLSFKRIINRPKRSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHH
GEKALKNLEEYAKKRQISLFDALCESDGGVGILTTKKAQNEANIFIQNIHTLKSYDNAKK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEHHHHHHHHHCCHHHH
VFDNIEELFKIKDYFSEQDDGDERIRNLDEFYANLREKLKEDPEASLEDLLSEISLLSDQ
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
DNLDEECVCLMSIHASKGLEFDYVFIIGFDEGFFPLNSEENLEEERRLAYVAITRAKKFL
CCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHEEECCCEEE
TISVANSRFYHGSRANINPSRFLEESKLINEKSKNQNIQKTSFCKGDLVKHKIFGIGRVV
EEEEECCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHCCCEEE
EVNKSGKEEKLNINFGGIMRVIMASFVEKAV
EECCCCCCCEEECCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNLFEDLNDSQKQAVSHIDGAMLILAGAGSGKTKTITTRLAYLIGEVGIPSHNTLTLTFT
CCCHHHCCHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEC
NKAASVMRHRALNFLQGNHNPLLCTFHKFGLLFLKLHFERLERKNSFIVIDTDDTKKIIK
CHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHH
DLIHDKNKDNVYDIIQYISYCKNEGKRVSNVFEDLNLLKEHNFEKYQNEYKFANYYRAYE
HHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
EYLLKQNFVDFDDLLLLSNLILENDINFAKEQSLLYNYITVDEYQDTNTLQYKILKNLCC
HHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHH
MHENITVVGDDDQSIYSWRGAKIENILNFQNDFKNVKLAKLEQNYRSVGTILQAANNLIS
HCCCEEEEECCCCHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHC
HNEQRLGKTLICTKDTGENIKILKNENEKDEGLYIAQEVKKLLNSGVEAKEIAILFRVNA
CCHHHCCCEEEEECCCCCCEEEEECCCCCCCCEEHHHHHHHHHHCCCCHHHEEEEEEHHH
LSRAIEEAFMKKQISYKLLSGMRFYERLEIKDLISYLRLILNPSDDLSFKRIINRPKRSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHH
GEKALKNLEEYAKKRQISLFDALCESDGGVGILTTKKAQNEANIFIQNIHTLKSYDNAKK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEHHHHHHHHHCCHHHH
VFDNIEELFKIKDYFSEQDDGDERIRNLDEFYANLREKLKEDPEASLEDLLSEISLLSDQ
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
DNLDEECVCLMSIHASKGLEFDYVFIIGFDEGFFPLNSEENLEEERRLAYVAITRAKKFL
CCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHEEECCCEEE
TISVANSRFYHGSRANINPSRFLEESKLINEKSKNQNIQKTSFCKGDLVKHKIFGIGRVV
EEEEECCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHCCCEEE
EVNKSGKEEKLNINFGGIMRVIMASFVEKAV
EECCCCCCCEEECCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA