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The map label for this gene is thiH [H]

Identifier: 57237930

GI number: 57237930

Start: 1105885

End: 1107030

Strand: Reverse

Name: thiH [H]

Synonym: CJE1188

Alternate gene names: 57237930

Gene position: 1107030-1105885 (Counterclockwise)

Preceding gene: 57237931

Following gene: 57237929

Centisome position: 62.27

GC content: 34.9

Gene sequence:

>1146_bases
ATGCAAGATTATATGCAGTATTTACCTCACATGCAGGAAATAAAAAGCGAAATTTTAAACAAGGTTTTAACTCAAGTTCA
AAGCTATGATGAGAGTCAATTTAGCGCCAAGGATGTTAAAAATGCTTTAAATCAAACGCATTTAAGTATAGAGCATTTAA
AAGCCTTGCTTTCAAGTGCAGCAGAAGATTTCATAGAAGAATTAGCCTTTAAATCCGCCAAAGTCAAGCAAAAATATTTT
GGAAATTCTATCTCTCTTTTTACTCCGCTTTATTTGTCAAATTATTGTAATTCTAAATGTGTTTATTGTGGTTTTCAAAA
AGGCAATAAAATCGCAAGAGCTAAGCTAAATGAAGCTGAAATTCACGAAGAAATGCAAGCCATTGCTAAAAGTGGTTTAG
AAGAAATTTTAATGCTAACAGGTGAGGGTAGGGAGTTTGCAAGTGTAGAATATATAGCAAATGCTTGCAAGATTGCTAGA
GAGTATTTTAAGGTTGTAGGTGTTGAAATTTATCCTATGAATGAAGATGAATATAAAATTTTGCATGAAAAGGGTTGTGA
TTATGTGACTGTTTTTCAAGAAACTTACAATCCTCTAAAATATTCTAAAATTCATTTAGCTGGTGAAAAACGCATTTTTC
CTTATCGCTTTAATGCTCAAGAAAGAGCTTTAAAAGCGGGTATGCGTGGAGTGGCTTTTGCGGCACTTTTGGGTATAGAT
GATTTTAGAAAAGACGCTCTTGCTACGGCGCTTCATGCACATTTTTTACAACAAGCTTATTCGCATGCTGAAATTTCTAT
TTCAGTGCCGCGTTTAAGACCTATTATCAATAATGCCAAAATCCATCCTAAAGATGTAAGTGAAAAACGCCTTTTGCAAG
TACTTTGTGCTTATAGGCTCTTTTTGCCTTTTGCGGGCATTACCATTTCAAGTCGTGAAAGAATAGGTTTTAGAGATGAG
GTTATCAAGCTTGGTGCTACTAAAATGAGTGCAGGAGTGAGTGTGGGTATAGGTGAGCATAAGGGTGAGAAAAAGGGTGA
TGAGCAGTTTGAAATTTCAGATGATAGAAGCGTAGATGAAATTTTAGCTATGCTTAAAAGATCCAATTTACAAGCTGTGA
TGAGCGATAGTATCTATGTGGGATAA

Upstream 100 bases:

>100_bases
TGGCGGTAAATGCAGGAAGAGCGGCTTTTTTAGCAGGTTTGGCAAGTGTGAGTGAAGCTAAAGCAAGTTCCCCATTAACA
GGCTTTTTAAGGGATTGATT

Downstream 100 bases:

>100_bases
AAAAATCATCGCCATAAGCGATAGAAAATGTGTAGAAATAGACTTTTTAAAGCAGATTGAAAAACTTGCTAAGGCTAAGG
TAGATGCTATAGTTTTAAGA

Product: thiamine biosynthesis protein ThiH

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Dehydroglycine synthase; Tyrosine lyase [H]

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MQDYMQYLPHMQEIKSEILNKVLTQVQSYDESQFSAKDVKNALNQTHLSIEHLKALLSSAAEDFIEELAFKSAKVKQKYF
GNSISLFTPLYLSNYCNSKCVYCGFQKGNKIARAKLNEAEIHEEMQAIAKSGLEEILMLTGEGREFASVEYIANACKIAR
EYFKVVGVEIYPMNEDEYKILHEKGCDYVTVFQETYNPLKYSKIHLAGEKRIFPYRFNAQERALKAGMRGVAFAALLGID
DFRKDALATALHAHFLQQAYSHAEISISVPRLRPIINNAKIHPKDVSEKRLLQVLCAYRLFLPFAGITISSRERIGFRDE
VIKLGATKMSAGVSVGIGEHKGEKKGDEQFEISDDRSVDEILAMLKRSNLQAVMSDSIYVG

Sequences:

>Translated_381_residues
MQDYMQYLPHMQEIKSEILNKVLTQVQSYDESQFSAKDVKNALNQTHLSIEHLKALLSSAAEDFIEELAFKSAKVKQKYF
GNSISLFTPLYLSNYCNSKCVYCGFQKGNKIARAKLNEAEIHEEMQAIAKSGLEEILMLTGEGREFASVEYIANACKIAR
EYFKVVGVEIYPMNEDEYKILHEKGCDYVTVFQETYNPLKYSKIHLAGEKRIFPYRFNAQERALKAGMRGVAFAALLGID
DFRKDALATALHAHFLQQAYSHAEISISVPRLRPIINNAKIHPKDVSEKRLLQVLCAYRLFLPFAGITISSRERIGFRDE
VIKLGATKMSAGVSVGIGEHKGEKKGDEQFEISDDRSVDEILAMLKRSNLQAVMSDSIYVG
>Mature_381_residues
MQDYMQYLPHMQEIKSEILNKVLTQVQSYDESQFSAKDVKNALNQTHLSIEHLKALLSSAAEDFIEELAFKSAKVKQKYF
GNSISLFTPLYLSNYCNSKCVYCGFQKGNKIARAKLNEAEIHEEMQAIAKSGLEEILMLTGEGREFASVEYIANACKIAR
EYFKVVGVEIYPMNEDEYKILHEKGCDYVTVFQETYNPLKYSKIHLAGEKRIFPYRFNAQERALKAGMRGVAFAALLGID
DFRKDALATALHAHFLQQAYSHAEISISVPRLRPIINNAKIHPKDVSEKRLLQVLCAYRLFLPFAGITISSRERIGFRDE
VIKLGATKMSAGVSVGIGEHKGEKKGDEQFEISDDRSVDEILAMLKRSNLQAVMSDSIYVG

Specific function: Catalyzes the radical-mediated cleavage of tyrosine to 2-iminoacetate and 4-cresol [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. ThiH family [H]

Homologues:

Organism=Escherichia coli, GI1790423, Length=350, Percent_Identity=37.4285714285714, Blast_Score=244, Evalue=5e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR007197
- InterPro:   IPR012726 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 43096; Mature: 43096

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDYMQYLPHMQEIKSEILNKVLTQVQSYDESQFSAKDVKNALNQTHLSIEHLKALLSSA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
AEDFIEELAFKSAKVKQKYFGNSISLFTPLYLSNYCNSKCVYCGFQKGNKIARAKLNEAE
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHCCCHHH
IHEEMQAIAKSGLEEILMLTGEGREFASVEYIANACKIAREYFKVVGVEIYPMNEDEYKI
HHHHHHHHHHHCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHH
LHEKGCDYVTVFQETYNPLKYSKIHLAGEKRIFPYRFNAQERALKAGMRGVAFAALLGID
HHHCCCCEEEEEHHHCCCCCEEEEEECCCCEECEEECCHHHHHHHHCCHHHHHHHHHHHH
DFRKDALATALHAHFLQQAYSHAEISISVPRLRPIINNAKIHPKDVSEKRLLQVLCAYRL
HHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
FLPFAGITISSRERIGFRDEVIKLGATKMSAGVSVGIGEHKGEKKGDEQFEISDDRSVDE
HHHHCCCEECCCCCCCHHHHHHHHCCHHHHCCCEECCCCCCCCCCCCCCEECCCCCCHHH
ILAMLKRSNLQAVMSDSIYVG
HHHHHHHCCHHHHHCCCCCCC
>Mature Secondary Structure
MQDYMQYLPHMQEIKSEILNKVLTQVQSYDESQFSAKDVKNALNQTHLSIEHLKALLSSA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
AEDFIEELAFKSAKVKQKYFGNSISLFTPLYLSNYCNSKCVYCGFQKGNKIARAKLNEAE
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHCCCHHH
IHEEMQAIAKSGLEEILMLTGEGREFASVEYIANACKIAREYFKVVGVEIYPMNEDEYKI
HHHHHHHHHHHCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHH
LHEKGCDYVTVFQETYNPLKYSKIHLAGEKRIFPYRFNAQERALKAGMRGVAFAALLGID
HHHCCCCEEEEEHHHCCCCCEEEEEECCCCEECEEECCHHHHHHHHCCHHHHHHHHHHHH
DFRKDALATALHAHFLQQAYSHAEISISVPRLRPIINNAKIHPKDVSEKRLLQVLCAYRL
HHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
FLPFAGITISSRERIGFRDEVIKLGATKMSAGVSVGIGEHKGEKKGDEQFEISDDRSVDE
HHHHCCCEECCCCCCCHHHHHHHHCCHHHHCCCEECCCCCCCCCCCCCCEECCCCCCHHH
ILAMLKRSNLQAVMSDSIYVG
HHHHHHHCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8432721; 8265357; 9278503; 10082377 [H]