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The map label for this gene is thiE [H]

Identifier: 57237929

GI number: 57237929

Start: 1105290

End: 1105895

Strand: Reverse

Name: thiE [H]

Synonym: CJE1187

Alternate gene names: 57237929

Gene position: 1105895-1105290 (Counterclockwise)

Preceding gene: 57237930

Following gene: 57237928

Centisome position: 62.2

GC content: 30.69

Gene sequence:

>606_bases
ATGTGGGATAAAAAAATCATCGCCATAAGCGATAGAAAATGTGTAGAAATAGACTTTTTAAAGCAGATTGAAAAACTTGC
TAAGGCTAAGGTAGATGCTATAGTTTTAAGAGAAAAAGATTTAAGCGAATTTGAGTATTATGACTTAGCAAAAGAAGTTT
TAAGCATTTGCGCAAAGCAAAAAGTGACTTGTTTTTTGCATTTTTTTGATAGGGAGTGTTTAAAATTGGGACATCGTTAT
TTTCACGCACCGCTTTCTTTGCTAAGAAAAGAACCTAAATTAACAAAATATTTTCATATACTTGGTACTTCAGTCCATAG
CAAAGAAGAGCTTTTAGAGGCGATGAGCTATAAGGTAAATTATGCTTTTGTAGGGCATATTTTTGAAAGTTCTTGCAAGA
TGGGTTTAGAACCTAAAGGGATTGATTTTTTAAAATCCTTGCTTGAATTTAGTCAAATTCCACTTTATGCTATAGGCGGT
ATCAATGCTCAAAATATAGAAAATTTTAAAGATATAAATGTAGTAGGTGTTTGCATGCGTGAAATTTTAATGAAAGAAAA
AGATCTTAAAAAGTATCTTTTAGAATGTAGGCAAAATTTACGATGA

Upstream 100 bases:

>100_bases
GGTGATGAGCAGTTTGAAATTTCAGATGATAGAAGCGTAGATGAAATTTTAGCTATGCTTAAAAGATCCAATTTACAAGC
TGTGATGAGCGATAGTATCT

Downstream 100 bases:

>100_bases
ATAAAATTCTTTCTTTACCAGAAGATTTAAAGCAATTAAAAGGTGTCAATTATAAAAAAATTAAATCTTGCACTTTTGCT
AAATATACACAAACGGATAC

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MWDKKIIAISDRKCVEIDFLKQIEKLAKAKVDAIVLREKDLSEFEYYDLAKEVLSICAKQKVTCFLHFFDRECLKLGHRY
FHAPLSLLRKEPKLTKYFHILGTSVHSKEELLEAMSYKVNYAFVGHIFESSCKMGLEPKGIDFLKSLLEFSQIPLYAIGG
INAQNIENFKDINVVGVCMREILMKEKDLKKYLLECRQNLR

Sequences:

>Translated_201_residues
MWDKKIIAISDRKCVEIDFLKQIEKLAKAKVDAIVLREKDLSEFEYYDLAKEVLSICAKQKVTCFLHFFDRECLKLGHRY
FHAPLSLLRKEPKLTKYFHILGTSVHSKEELLEAMSYKVNYAFVGHIFESSCKMGLEPKGIDFLKSLLEFSQIPLYAIGG
INAQNIENFKDINVVGVCMREILMKEKDLKKYLLECRQNLR
>Mature_201_residues
MWDKKIIAISDRKCVEIDFLKQIEKLAKAKVDAIVLREKDLSEFEYYDLAKEVLSICAKQKVTCFLHFFDRECLKLGHRY
FHAPLSLLRKEPKLTKYFHILGTSVHSKEELLEAMSYKVNYAFVGHIFESSCKMGLEPKGIDFLKSLLEFSQIPLYAIGG
INAQNIENFKDINVVGVCMREILMKEKDLKKYLLECRQNLR

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 23462; Mature: 23462

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWDKKIIAISDRKCVEIDFLKQIEKLAKAKVDAIVLREKDLSEFEYYDLAKEVLSICAKQ
CCCCEEEEECCCCEEHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHH
KVTCFLHFFDRECLKLGHRYFHAPLSLLRKEPKLTKYFHILGTSVHSKEELLEAMSYKVN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHH
YAFVGHIFESSCKMGLEPKGIDFLKSLLEFSQIPLYAIGGINAQNIENFKDINVVGVCMR
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEECCCCHHHCCCHHHHHHHHHHHH
EILMKEKDLKKYLLECRQNLR
HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MWDKKIIAISDRKCVEIDFLKQIEKLAKAKVDAIVLREKDLSEFEYYDLAKEVLSICAKQ
CCCCEEEEECCCCEEHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHH
KVTCFLHFFDRECLKLGHRYFHAPLSLLRKEPKLTKYFHILGTSVHSKEELLEAMSYKVN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHH
YAFVGHIFESSCKMGLEPKGIDFLKSLLEFSQIPLYAIGGINAQNIENFKDINVVGVCMR
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEECCCCHHHCCCHHHHHHHHHHHH
EILMKEKDLKKYLLECRQNLR
HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12552129 [H]