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The map label for this gene is thiG [H]

Identifier: 57237931

GI number: 57237931

Start: 1107033

End: 1107809

Strand: Reverse

Name: thiG [H]

Synonym: CJE1189

Alternate gene names: 57237931

Gene position: 1107809-1107033 (Counterclockwise)

Preceding gene: 57237932

Following gene: 57237930

Centisome position: 62.31

GC content: 39.0

Gene sequence:

>777_bases
ATGCAAGAAAAATTAAAAAATGATAAATTAAAAATAGGCAAATACGAGTTTGATTCAAGATTTATTTTAGGTTCTGGAAA
ATATTCGCTAGAGCTTATAAAATCAGCTATAGAAGAAGCTAAAGCGCAGATCATCACTCTAGCCTTACGCCGTGCAAATA
CAGGGGAAATTGCTAATATACTTGATTATATCCCTAAAAATATCACACTTTTACCCAACACTTCAGGCGCAAGAAACGCC
GAAGAAGCCTTACGCATTGCAAGGCTTTCAAGAGAGCTTGGTTGTGGAGAACTTATCAAGATAGAAGTGATAAGCGATAG
TAGGTATTTATTGCCTGATAATTATGAAACGATTAAAGCTTGCGAGCTTTTAGCTAAAGAGGGTTTTACACCCTTACCTT
ACATGCATGCTGATCTTTACGCAGCTAGAGCTATGCGTGATGCTGGAGCTGCGGCTATCATGCCTTTAGCAGCGCCTATT
GGGAGTAATAAAGGCTTATGTGCTAAGGAATTTATACAAATTTTGCTTAATGAAATTGATTTGCCTATTATTGTAGATGC
GGGTATTGGAAGTCCTTCACAAGCTTGTGAAGCTATGCAAATGGGAGTAAGTGCGGTGATGGTAAATACAGCCATAGCTG
AAGCAAAAGATATAGCCTTGATGGCTAGAGCTTTTTCTTTGGCGGTAAATGCAGGAAGAGCGGCTTTTTTAGCAGGTTTG
GCAAGTGTGAGTGAAGCTAAAGCAAGTTCCCCATTAACAGGCTTTTTAAGGGATTGA

Upstream 100 bases:

>100_bases
GTGCTAAGCTAGGAAATGGACTTATGGCGCCAAGGGTAAATATTTGCGCAGGACATCAAAGTAACCTTGTTTTAGAACTT
TTAGCAAATAAGGAGTAAAA

Downstream 100 bases:

>100_bases
TTATGCAAGATTATATGCAGTATTTACCTCACATGCAGGAAATAAAAAGCGAAATTTTAAACAAGGTTTTAACTCAAGTT
CAAAGCTATGATGAGAGTCA

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MQEKLKNDKLKIGKYEFDSRFILGSGKYSLELIKSAIEEAKAQIITLALRRANTGEIANILDYIPKNITLLPNTSGARNA
EEALRIARLSRELGCGELIKIEVISDSRYLLPDNYETIKACELLAKEGFTPLPYMHADLYAARAMRDAGAAAIMPLAAPI
GSNKGLCAKEFIQILLNEIDLPIIVDAGIGSPSQACEAMQMGVSAVMVNTAIAEAKDIALMARAFSLAVNAGRAAFLAGL
ASVSEAKASSPLTGFLRD

Sequences:

>Translated_258_residues
MQEKLKNDKLKIGKYEFDSRFILGSGKYSLELIKSAIEEAKAQIITLALRRANTGEIANILDYIPKNITLLPNTSGARNA
EEALRIARLSRELGCGELIKIEVISDSRYLLPDNYETIKACELLAKEGFTPLPYMHADLYAARAMRDAGAAAIMPLAAPI
GSNKGLCAKEFIQILLNEIDLPIIVDAGIGSPSQACEAMQMGVSAVMVNTAIAEAKDIALMARAFSLAVNAGRAAFLAGL
ASVSEAKASSPLTGFLRD
>Mature_258_residues
MQEKLKNDKLKIGKYEFDSRFILGSGKYSLELIKSAIEEAKAQIITLALRRANTGEIANILDYIPKNITLLPNTSGARNA
EEALRIARLSRELGCGELIKIEVISDSRYLLPDNYETIKACELLAKEGFTPLPYMHADLYAARAMRDAGAAAIMPLAAPI
GSNKGLCAKEFIQILLNEIDLPIIVDAGIGSPSQACEAMQMGVSAVMVNTAIAEAKDIALMARAFSLAVNAGRAAFLAGL
ASVSEAKASSPLTGFLRD

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family [H]

Homologues:

Organism=Escherichia coli, GI48994993, Length=251, Percent_Identity=47.0119521912351, Blast_Score=211, Evalue=3e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR008867 [H]

Pfam domain/function: PF05690 ThiG [H]

EC number: NA

Molecular weight: Translated: 27662; Mature: 27662

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEKLKNDKLKIGKYEFDSRFILGSGKYSLELIKSAIEEAKAQIITLALRRANTGEIANI
CCCCCCCCCEEECEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
LDYIPKNITLLPNTSGARNAEEALRIARLSRELGCGELIKIEVISDSRYLLPDNYETIKA
HHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCEECCCCCHHHHH
CELLAKEGFTPLPYMHADLYAARAMRDAGAAAIMPLAAPIGSNKGLCAKEFIQILLNEID
HHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHCC
LPIIVDAGIGSPSQACEAMQMGVSAVMVNTAIAEAKDIALMARAFSLAVNAGRAAFLAGL
CCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
ASVSEAKASSPLTGFLRD
HHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MQEKLKNDKLKIGKYEFDSRFILGSGKYSLELIKSAIEEAKAQIITLALRRANTGEIANI
CCCCCCCCCEEECEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
LDYIPKNITLLPNTSGARNAEEALRIARLSRELGCGELIKIEVISDSRYLLPDNYETIKA
HHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCEECCCCCHHHHH
CELLAKEGFTPLPYMHADLYAARAMRDAGAAAIMPLAAPIGSNKGLCAKEFIQILLNEID
HHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHCC
LPIIVDAGIGSPSQACEAMQMGVSAVMVNTAIAEAKDIALMARAFSLAVNAGRAAFLAGL
CCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
ASVSEAKASSPLTGFLRD
HHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA