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The map label for this gene is hemK [H]

Identifier: 57237024

GI number: 57237024

Start: 756238

End: 757053

Strand: Reverse

Name: hemK [H]

Synonym: CJE0822

Alternate gene names: 57237024

Gene position: 757053-756238 (Counterclockwise)

Preceding gene: 57237025

Following gene: 57237023

Centisome position: 42.58

GC content: 26.47

Gene sequence:

>816_bases
TTGACTATAAAAAATGCTCTTATGGAAGCTAAATCTAGCTTAAAAGGTTATGAAAATGAAGCAGTATTTATACTCTGTGA
ATATCTAAAAAAAGATAAAGCTTGGTTATTTTTAAACCAAGATATACAAATAGATCATGAACCTTATTTTAAGCTTATAA
GGCGTTTTAAATCTGGAGAACCTTTTGAATATATATTTGAAAAAGTGGATTTTTGGGGCTTAGAATTTAAGATAAAAAAA
GGCATTCTAATCCCTCGCTACGATAGTGAAATTTTACTTTTTCAAATTTTAAATTTATGCAAAAAAAATACTTTTAATGG
GATTTTAGAAATAGGATTTGGAAGTGGAATTTTAAGCATTATTTTAGCCAAAGAACTTGGGTTAAAAATCACAGCCTGCG
ATATCAATCTCAAAGCCTTAGAATTAGCTTTAGAGAATGCCAAATTACACAAAGTCGATCACTTGATTGACTTTAAACTT
TGTAATTTTAAGCAAATTAAAGAAAATTATGATTTTATTTTTTCAAATCCACCCTATATTAAAAATTCATATCCTATAGA
TATTTGGGTGCAAAAAGAACCCAAAGAGGCTTTATTTGGAGGAGAAAAAGGATATGAAATTCTAGAAGAAATCATACATT
TTTCCCTTGATAAAAAAGTTAAATTTTTAGCTTGCGAATTTGGCTATGATCAAAAAGAAATTTTGGAAAAAATTTTATAT
CAAAATAATTTCATTGTTGATTTTTTCAAAGATGAACAAGGTTATAATCGTGCTTTTGTAGCTAAATTTACAAATATGCG
TTATGATAAAAAATAA

Upstream 100 bases:

>100_bases
CCCTAGCAAGAGAAAATAAAGCTTTTATCAAAACAAGTAAAATATATGCATTTTTTTATCTCAGCCATCCAAGTATTAGT
GATAGAATCAAGGCTCTTTC

Downstream 100 bases:

>100_bases
AAGGAGAAACTCTTGAAAGCTGTTAATTTATTTTTACTTGCCTCCATCATAGGGGTTGAACTCATACTAGGAATAGTTGT
TGCGCCAACAATTTTTTTTC

Product: HemK family modification methylase

Products: NA

Alternate protein names: M.AaoHemKP [H]

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MTIKNALMEAKSSLKGYENEAVFILCEYLKKDKAWLFLNQDIQIDHEPYFKLIRRFKSGEPFEYIFEKVDFWGLEFKIKK
GILIPRYDSEILLFQILNLCKKNTFNGILEIGFGSGILSIILAKELGLKITACDINLKALELALENAKLHKVDHLIDFKL
CNFKQIKENYDFIFSNPPYIKNSYPIDIWVQKEPKEALFGGEKGYEILEEIIHFSLDKKVKFLACEFGYDQKEILEKILY
QNNFIVDFFKDEQGYNRAFVAKFTNMRYDKK

Sequences:

>Translated_271_residues
MTIKNALMEAKSSLKGYENEAVFILCEYLKKDKAWLFLNQDIQIDHEPYFKLIRRFKSGEPFEYIFEKVDFWGLEFKIKK
GILIPRYDSEILLFQILNLCKKNTFNGILEIGFGSGILSIILAKELGLKITACDINLKALELALENAKLHKVDHLIDFKL
CNFKQIKENYDFIFSNPPYIKNSYPIDIWVQKEPKEALFGGEKGYEILEEIIHFSLDKKVKFLACEFGYDQKEILEKILY
QNNFIVDFFKDEQGYNRAFVAKFTNMRYDKK
>Mature_270_residues
TIKNALMEAKSSLKGYENEAVFILCEYLKKDKAWLFLNQDIQIDHEPYFKLIRRFKSGEPFEYIFEKVDFWGLEFKIKKG
ILIPRYDSEILLFQILNLCKKNTFNGILEIGFGSGILSIILAKELGLKITACDINLKALELALENAKLHKVDHLIDFKLC
NFKQIKENYDFIFSNPPYIKNSYPIDIWVQKEPKEALFGGEKGYEILEEIIHFSLDKKVKFLACEFGYDQKEILEKILYQ
NNFIVDFFKDEQGYNRAFVAKFTNMRYDKK

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Escherichia coli, GI1787463, Length=249, Percent_Identity=27.710843373494, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI87082085, Length=195, Percent_Identity=28.2051282051282, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 31948; Mature: 31816

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIKNALMEAKSSLKGYENEAVFILCEYLKKDKAWLFLNQDIQIDHEPYFKLIRRFKSGE
CCHHHHHHHHHHHHCCCCCCEEEEEEHHHHCCCEEEEECCCEEECCCHHHHHHHHHCCCC
PFEYIFEKVDFWGLEFKIKKGILIPRYDSEILLFQILNLCKKNTFNGILEIGFGSGILSI
CHHHHHHHHHHCCEEEEEECCEEEECCCCHHHHHHHHHHHHCCCCCCEEEECCCCHHHHH
ILAKELGLKITACDINLKALELALENAKLHKVDHLIDFKLCNFKQIKENYDFIFSNPPYI
HHHHHHCCEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHCCCCEEECCCCCC
KNSYPIDIWVQKEPKEALFGGEKGYEILEEIIHFSLDKKVKFLACEFGYDQKEILEKILY
CCCCCEEEEEECCCHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHH
QNNFIVDFFKDEQGYNRAFVAKFTNMRYDKK
CCCEEEEEEECCCCCCEEEEEHHHCCCCCCC
>Mature Secondary Structure 
TIKNALMEAKSSLKGYENEAVFILCEYLKKDKAWLFLNQDIQIDHEPYFKLIRRFKSGE
CHHHHHHHHHHHHCCCCCCEEEEEEHHHHCCCEEEEECCCEEECCCHHHHHHHHHCCCC
PFEYIFEKVDFWGLEFKIKKGILIPRYDSEILLFQILNLCKKNTFNGILEIGFGSGILSI
CHHHHHHHHHHCCEEEEEECCEEEECCCCHHHHHHHHHHHHCCCCCCEEEECCCCHHHHH
ILAKELGLKITACDINLKALELALENAKLHKVDHLIDFKLCNFKQIKENYDFIFSNPPYI
HHHHHHCCEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHCCCCEEECCCCCC
KNSYPIDIWVQKEPKEALFGGEKGYEILEEIIHFSLDKKVKFLACEFGYDQKEILEKILY
CCCCCEEEEEECCCHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHH
QNNFIVDFFKDEQGYNRAFVAKFTNMRYDKK
CCCEEEEEEECCCCCCEEEEEHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]