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The map label for this gene is nuoF2 [H]

Identifier: 57235004

GI number: 57235004

Start: 141870

End: 143123

Strand: Direct

Name: nuoF2 [H]

Synonym: DET0146

Alternate gene names: 57235004

Gene position: 141870-143123 (Clockwise)

Preceding gene: 57235005

Following gene: 57235003

Centisome position: 9.65

GC content: 52.87

Gene sequence:

>1254_bases
ATGCAAGACATTAAGATTATTACCCGTCATCTGAATACCGCCAGCTCAGCTGATATTGATGTATATATGGCTGACGGCGG
CTACCAGGCCCTTAAAAAAGCCCTGTCCATGACTCCGGACGAAGTAATTGCCGAAGTCAAACGCTCAAAGCTGGTGGGTC
GGGGTGGAGCGGCCTTTCCTACCGGGCTCAAGTGGGAGCTTACCCGCAAGGAAAAGGCTAATCCGAAATATATCGTCTGT
AATGCCAGCGAGGGTGAACCGGGCACTTTTAAAGACCGGCTGATACTTAAAAACGACCCCCACATGGTGCTGGAAGGTTT
TATTATTGCCGCTTATGCGGTGGGTACTTCCCAGGGCTTTATCCATGCCCGTGAGGTTTATACCGAAGAAATAGAGCTGT
TCCAGAAAGCCATAGACCAGGCAACCGAACGCGGTTTTCTGGGGCAGAATATCATGGGGAGCAATTTCTCGCTGGATATC
CAGTTTTACAAGAGCGCCGGTGCTTATATCTGCGGTGAGGAAACCGCCCTTTTTGAATCCCTTGAAGGGCACAGAGGTAT
TCCCGCCGCCCGCCCGCCGTATCCGGTGCAGGTGGGGCTTATGGATAAACCCACCACTGTTAATAACGTAGAAACTCTCT
GCAATGTGCCTGCCATTATAAATAACGGGGCAGACTGGTTTGCTGCAATCGGCCATCCGGACTATCCCGGCACCAAGCTC
TTCTGCCTTTCGGGTAACGTGAAAGAGCCGGGTGTTTTTGAAATGCCGCTGGGAACCAACCTTAAGGACTTGCTTGAGGC
CGGGGGAGGCGTAGACGGCAAGTTCAAGGCGGTTTTGCCGGGGGGCATATCTTCCAGCCTGCTTACCGAAATGGACGTCA
ATCTGGACTTTAAGACGCTGGCCAAGGCTGGTACTATGCTGGGGTCAGGTGCAGTTATTGTGATAAACTCCGATACCAGC
ATGGTAAATGTAGCTTCCAACGTAGCCCACTTCTTTGACGAAGAGGGTTGCGGCAAGTGCTCTATCTGCCGTGAAGGCAC
CCGCCGGGCGGCTGAAATCCTTAGCCGCTTTTCCCGCGGGCAGGGCAACCGCAACGAACTGGAGTGGCTGCTGGAACTGC
ACGAAGTAATGAAAGATACCGCTTCCTGCGGTCTGGGACAGGTAGCCTTAAATGTGGCTGCCAGTGCCATTCGCAACTTC
AAGGGTGAATTCTTGGCGCAGGTGCGTAAGGAGGAAGGCGTATGGTTACGCTAA

Upstream 100 bases:

>100_bases
TGTGACCAGTCACCGGCTATGGTAATAAACGGGGTGGTTTACGGAAAGCTGACTGCTCAGCTGGTAACCGAAGTCCTGGA
CGAATTGAGGACTTACTAAA

Downstream 100 bases:

>100_bases
ATATTGACAACAAGCAGATAAGCGTACCTGAAGGTACGACTATTATGCAGGCCGCCAAGCAGGCCAATATCAATATCCCC
CACCTGTGTTATTTTGAAGG

Product: [Fe] hydrogenase, HymB subunit, putative

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MQDIKIITRHLNTASSADIDVYMADGGYQALKKALSMTPDEVIAEVKRSKLVGRGGAAFPTGLKWELTRKEKANPKYIVC
NASEGEPGTFKDRLILKNDPHMVLEGFIIAAYAVGTSQGFIHAREVYTEEIELFQKAIDQATERGFLGQNIMGSNFSLDI
QFYKSAGAYICGEETALFESLEGHRGIPAARPPYPVQVGLMDKPTTVNNVETLCNVPAIINNGADWFAAIGHPDYPGTKL
FCLSGNVKEPGVFEMPLGTNLKDLLEAGGGVDGKFKAVLPGGISSSLLTEMDVNLDFKTLAKAGTMLGSGAVIVINSDTS
MVNVASNVAHFFDEEGCGKCSICREGTRRAAEILSRFSRGQGNRNELEWLLELHEVMKDTASCGLGQVALNVAASAIRNF
KGEFLAQVRKEEGVWLR

Sequences:

>Translated_417_residues
MQDIKIITRHLNTASSADIDVYMADGGYQALKKALSMTPDEVIAEVKRSKLVGRGGAAFPTGLKWELTRKEKANPKYIVC
NASEGEPGTFKDRLILKNDPHMVLEGFIIAAYAVGTSQGFIHAREVYTEEIELFQKAIDQATERGFLGQNIMGSNFSLDI
QFYKSAGAYICGEETALFESLEGHRGIPAARPPYPVQVGLMDKPTTVNNVETLCNVPAIINNGADWFAAIGHPDYPGTKL
FCLSGNVKEPGVFEMPLGTNLKDLLEAGGGVDGKFKAVLPGGISSSLLTEMDVNLDFKTLAKAGTMLGSGAVIVINSDTS
MVNVASNVAHFFDEEGCGKCSICREGTRRAAEILSRFSRGQGNRNELEWLLELHEVMKDTASCGLGQVALNVAASAIRNF
KGEFLAQVRKEEGVWLR
>Mature_417_residues
MQDIKIITRHLNTASSADIDVYMADGGYQALKKALSMTPDEVIAEVKRSKLVGRGGAAFPTGLKWELTRKEKANPKYIVC
NASEGEPGTFKDRLILKNDPHMVLEGFIIAAYAVGTSQGFIHAREVYTEEIELFQKAIDQATERGFLGQNIMGSNFSLDI
QFYKSAGAYICGEETALFESLEGHRGIPAARPPYPVQVGLMDKPTTVNNVETLCNVPAIINNGADWFAAIGHPDYPGTKL
FCLSGNVKEPGVFEMPLGTNLKDLLEAGGGVDGKFKAVLPGGISSSLLTEMDVNLDFKTLAKAGTMLGSGAVIVINSDTS
MVNVASNVAHFFDEEGCGKCSICREGTRRAAEILSRFSRGQGNRNELEWLLELHEVMKDTASCGLGQVALNVAASAIRNF
KGEFLAQVRKEEGVWLR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI20149568, Length=396, Percent_Identity=40.9090909090909, Blast_Score=301, Evalue=7e-82,
Organism=Homo sapiens, GI260656005, Length=396, Percent_Identity=40.9090909090909, Blast_Score=301, Evalue=9e-82,
Organism=Escherichia coli, GI1788620, Length=400, Percent_Identity=43.5, Blast_Score=339, Evalue=2e-94,
Organism=Caenorhabditis elegans, GI17531727, Length=406, Percent_Identity=40.3940886699507, Blast_Score=307, Evalue=7e-84,
Organism=Drosophila melanogaster, GI19920794, Length=390, Percent_Identity=39.4871794871795, Blast_Score=290, Evalue=2e-78,
Organism=Drosophila melanogaster, GI45550452, Length=397, Percent_Identity=39.5465994962217, Blast_Score=286, Evalue=2e-77,
Organism=Drosophila melanogaster, GI24653819, Length=385, Percent_Identity=36.6233766233766, Blast_Score=264, Evalue=8e-71,
Organism=Drosophila melanogaster, GI24653817, Length=347, Percent_Identity=36.3112391930836, Blast_Score=237, Evalue=9e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 45164; Mature: 45164

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDIKIITRHLNTASSADIDVYMADGGYQALKKALSMTPDEVIAEVKRSKLVGRGGAAFP
CCCHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCC
TGLKWELTRKEKANPKYIVCNASEGEPGTFKDRLILKNDPHMVLEGFIIAAYAVGTSQGF
CCCEEEECCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHEEEEECCCCCC
IHAREVYTEEIELFQKAIDQATERGFLGQNIMGSNFSLDIQFYKSAGAYICGEETALFES
EEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCEEECCHHHHHHH
LEGHRGIPAARPPYPVQVGLMDKPTTVNNVETLCNVPAIINNGADWFAAIGHPDYPGTKL
HCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHCCCHHCCCCCCEEEECCCCCCCCCEE
FCLSGNVKEPGVFEMPLGTNLKDLLEAGGGVDGKFKAVLPGGISSSLLTEMDVNLDFKTL
EEEECCCCCCCEEECCCCCCHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHCCCCCHHHH
AKAGTMLGSGAVIVINSDTSMVNVASNVAHFFDEEGCGKCSICREGTRRAAEILSRFSRG
HHHHHEECCCEEEEEECCCCEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCC
QGNRNELEWLLELHEVMKDTASCGLGQVALNVAASAIRNFKGEFLAQVRKEEGVWLR
CCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MQDIKIITRHLNTASSADIDVYMADGGYQALKKALSMTPDEVIAEVKRSKLVGRGGAAFP
CCCHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCC
TGLKWELTRKEKANPKYIVCNASEGEPGTFKDRLILKNDPHMVLEGFIIAAYAVGTSQGF
CCCEEEECCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHEEEEECCCCCC
IHAREVYTEEIELFQKAIDQATERGFLGQNIMGSNFSLDIQFYKSAGAYICGEETALFES
EEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCEEECCHHHHHHH
LEGHRGIPAARPPYPVQVGLMDKPTTVNNVETLCNVPAIINNGADWFAAIGHPDYPGTKL
HCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHCCCHHCCCCCCEEEECCCCCCCCCEE
FCLSGNVKEPGVFEMPLGTNLKDLLEAGGGVDGKFKAVLPGGISSSLLTEMDVNLDFKTL
EEEECCCCCCCEEECCCCCCHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHCCCCCHHHH
AKAGTMLGSGAVIVINSDTSMVNVASNVAHFFDEEGCGKCSICREGTRRAAEILSRFSRG
HHHHHEECCCEEEEEECCCCEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCC
QGNRNELEWLLELHEVMKDTASCGLGQVALNVAASAIRNFKGEFLAQVRKEEGVWLR
CCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]