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The map label for this gene is nuoE [H]

Identifier: 57235005

GI number: 57235005

Start: 141401

End: 141868

Strand: Direct

Name: nuoE [H]

Synonym: DET0145

Alternate gene names: 57235005

Gene position: 141401-141868 (Clockwise)

Preceding gene: 57235015

Following gene: 57235004

Centisome position: 9.62

GC content: 47.01

Gene sequence:

>468_bases
ATGGAAAGTATCTGGAAAGAAAAAGTAGACGGCGTCATTACCCAGAGCGGAAGCTCTCGGCTCTCTCTCCTTCCATGTTT
GGAAGCGGTTCAGGAAGAATGTGGCTATATACCCCATGAAGCTGTAAATTATTTGAGGGAATGCTTAAGCATTCCCTCTA
TTGATATTTACGGCATGATTACCTTTTATAGCCTGCTCTCCACCAACCAAAAGGGAAAGTACGTAATTCGCCTGTGTAAT
TCGCTGCCCTGTTACCTGAACGGTACAGAAAACATCCTGGATACCCTGGTGGATAATCTGGGTATCGAACCCGGTCAAAC
CACTCTGGACCAACGTTTTACCCTGGAGCTGGTCCCCTGTTTGGGATTATGTGACCAGTCACCGGCTATGGTAATAAACG
GGGTGGTTTACGGAAAGCTGACTGCTCAGCTGGTAACCGAAGTCCTGGACGAATTGAGGACTTACTAA

Upstream 100 bases:

>100_bases
ATTATTATTCTGATTTGCTAATTTGATATCTTTTGCCGTTATATGCTAGTATTTAAAGGTCTTTTACGCCCCCTATTAAA
ATAACCGGAGTTACTTCAAT

Downstream 100 bases:

>100_bases
AATGCAAGACATTAAGATTATTACCCGTCATCTGAATACCGCCAGCTCAGCTGATATTGATGTATATATGGCTGACGGCG
GCTACCAGGCCCTTAAAAAA

Product: [Fe] hydrogenase, HymA subunit, putative

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E; NDH-1 subunit E; NUO5 [H]

Number of amino acids: Translated: 155; Mature: 155

Protein sequence:

>155_residues
MESIWKEKVDGVITQSGSSRLSLLPCLEAVQEECGYIPHEAVNYLRECLSIPSIDIYGMITFYSLLSTNQKGKYVIRLCN
SLPCYLNGTENILDTLVDNLGIEPGQTTLDQRFTLELVPCLGLCDQSPAMVINGVVYGKLTAQLVTEVLDELRTY

Sequences:

>Translated_155_residues
MESIWKEKVDGVITQSGSSRLSLLPCLEAVQEECGYIPHEAVNYLRECLSIPSIDIYGMITFYSLLSTNQKGKYVIRLCN
SLPCYLNGTENILDTLVDNLGIEPGQTTLDQRFTLELVPCLGLCDQSPAMVINGVVYGKLTAQLVTEVLDELRTY
>Mature_155_residues
MESIWKEKVDGVITQSGSSRLSLLPCLEAVQEECGYIPHEAVNYLRECLSIPSIDIYGMITFYSLLSTNQKGKYVIRLCN
SLPCYLNGTENILDTLVDNLGIEPGQTTLDQRFTLELVPCLGLCDQSPAMVINGVVYGKLTAQLVTEVLDELRTY

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=138, Percent_Identity=37.6811594202899, Blast_Score=108, Evalue=3e-24,
Organism=Escherichia coli, GI1788621, Length=148, Percent_Identity=35.1351351351351, Blast_Score=110, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17561328, Length=151, Percent_Identity=34.4370860927152, Blast_Score=103, Evalue=3e-23,
Organism=Drosophila melanogaster, GI18859877, Length=151, Percent_Identity=33.7748344370861, Blast_Score=110, Evalue=2e-25,
Organism=Drosophila melanogaster, GI21355353, Length=133, Percent_Identity=32.3308270676692, Blast_Score=89, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 17228; Mature: 17228

Theoretical pI: Translated: 4.19; Mature: 4.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
4.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESIWKEKVDGVITQSGSSRLSLLPCLEAVQEECGYIPHEAVNYLRECLSIPSIDIYGMI
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHH
TFYSLLSTNQKGKYVIRLCNSLPCYLNGTENILDTLVDNLGIEPGQTTLDQRFTLELVPC
HHHHHHHCCCCCHHHHHHHCCCCCEECCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
LGLCDQSPAMVINGVVYGKLTAQLVTEVLDELRTY
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MESIWKEKVDGVITQSGSSRLSLLPCLEAVQEECGYIPHEAVNYLRECLSIPSIDIYGMI
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHH
TFYSLLSTNQKGKYVIRLCNSLPCYLNGTENILDTLVDNLGIEPGQTTLDQRFTLELVPC
HHHHHHHCCCCCHHHHHHHCCCCCEECCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
LGLCDQSPAMVINGVVYGKLTAQLVTEVLDELRTY
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]