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The map label for this gene is fumB [H]

Identifier: 57234727

GI number: 57234727

Start: 430164

End: 430730

Strand: Direct

Name: fumB [H]

Synonym: DET0454

Alternate gene names: 57234727

Gene position: 430164-430730 (Clockwise)

Preceding gene: 57234728

Following gene: 57234719

Centisome position: 29.27

GC content: 53.26

Gene sequence:

>567_bases
GTGATATCCGGTATAAAACTGAATTCCCCGTTTAACCCTGCTGAACTGGAAAAGCTGCAAGCCGGAGATAGGGTGCTGAT
ATCCGGTGTTATATATGCCGCCCGTGATGCCGCCCATAAGCGGCTGGTGGAAGCTCTTAATCAGGGCAAACCGCTTCCTT
TTGACCTTAAAGGCCAGACTGTTTACTACATGGGGCCTTCGCCTGCCAAACCGGGTGAGGTTATAGGTTCAGCTGGCCCT
ACCACCAGTAGCCGGATGGACCGCTATACCCCGGAACTGCTGGATGCCGGGTTGCTGGCTATTATCGGCAAGGGCAACCG
TTCGGCTGAGGTCAGCCGGGCTATTGTAGGCAAAAAGGCGGTTTATTTCATATCTATAGGAGGGGCAGGGGCACTTCTCT
CACAGCGTATCAAAGAAAGCCGGATGGTGGCTTACCCTGAACTTGGGGCGGAAGCTATACTGGCGCTAAAGGTGGAGGAT
TTTCCGGCTATAGTGGCTATTGACTCTCAGGGGAACAACGCTTTTACTCTTGGTCAGTCCATTTACCGCACGGCTAAGGA
GGCCTGA

Upstream 100 bases:

>100_bases
CCCTGGGAGTGATGGCAGAAGCCGCACCCACCCATATTGCCAGTTTGCCGGTGGCGGTAAACCTGCAGTGCCATAGCGCC
CGCCATGGTGAGGTGGTGCT

Downstream 100 bases:

>100_bases
TATGAGTTGTATATTTTGCCAGATTGTCAAAGGGGAGATACCTGCCCAGATAGTTTACAAAGATGAAGATTTGGTGGCTT
TCAAAGATATAAATCCCCAG

Product: fumarate hydratase, beta subunit, putative

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 188; Mature: 188

Protein sequence:

>188_residues
MISGIKLNSPFNPAELEKLQAGDRVLISGVIYAARDAAHKRLVEALNQGKPLPFDLKGQTVYYMGPSPAKPGEVIGSAGP
TTSSRMDRYTPELLDAGLLAIIGKGNRSAEVSRAIVGKKAVYFISIGGAGALLSQRIKESRMVAYPELGAEAILALKVED
FPAIVAIDSQGNNAFTLGQSIYRTAKEA

Sequences:

>Translated_188_residues
MISGIKLNSPFNPAELEKLQAGDRVLISGVIYAARDAAHKRLVEALNQGKPLPFDLKGQTVYYMGPSPAKPGEVIGSAGP
TTSSRMDRYTPELLDAGLLAIIGKGNRSAEVSRAIVGKKAVYFISIGGAGALLSQRIKESRMVAYPELGAEAILALKVED
FPAIVAIDSQGNNAFTLGQSIYRTAKEA
>Mature_188_residues
MISGIKLNSPFNPAELEKLQAGDRVLISGVIYAARDAAHKRLVEALNQGKPLPFDLKGQTVYYMGPSPAKPGEVIGSAGP
TTSSRMDRYTPELLDAGLLAIIGKGNRSAEVSRAIVGKKAVYFISIGGAGALLSQRIKESRMVAYPELGAEAILALKVED
FPAIVAIDSQGNNAFTLGQSIYRTAKEA

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1838

COG function: function code C; Tartrate dehydratase beta subunit/Fumarate hydratase class I, C-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=176, Percent_Identity=42.0454545454545, Blast_Score=130, Evalue=4e-32,
Organism=Escherichia coli, GI1787897, Length=175, Percent_Identity=40.5714285714286, Blast_Score=128, Evalue=2e-31,
Organism=Escherichia coli, GI1789443, Length=181, Percent_Identity=30.3867403314917, Blast_Score=104, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 19917; Mature: 19917

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISGIKLNSPFNPAELEKLQAGDRVLISGVIYAARDAAHKRLVEALNQGKPLPFDLKGQT
CCCCEECCCCCCHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCE
VYYMGPSPAKPGEVIGSAGPTTSSRMDRYTPELLDAGLLAIIGKGNRSAEVSRAIVGKKA
EEEECCCCCCCCHHCCCCCCCCCHHHHHCCHHHHCCCEEEEEECCCCCHHHHHHHHCCCE
VYFISIGGAGALLSQRIKESRMVAYPELGAEAILALKVEDFPAIVAIDSQGNNAFTLGQS
EEEEEECCHHHHHHHHHHHHHEEECCCCCCCEEEEEEECCCCEEEEEECCCCCEEEHHHH
IYRTAKEA
HHHHHHCC
>Mature Secondary Structure
MISGIKLNSPFNPAELEKLQAGDRVLISGVIYAARDAAHKRLVEALNQGKPLPFDLKGQT
CCCCEECCCCCCHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCE
VYYMGPSPAKPGEVIGSAGPTTSSRMDRYTPELLDAGLLAIIGKGNRSAEVSRAIVGKKA
EEEECCCCCCCCHHCCCCCCCCCHHHHHCCHHHHCCCEEEEEECCCCCHHHHHHHHCCCE
VYFISIGGAGALLSQRIKESRMVAYPELGAEAILALKVEDFPAIVAIDSQGNNAFTLGQS
EEEEEECCHHHHHHHHHHHHHEEECCCCCCCEEEEEEECCCCEEEEEECCCCCEEEHHHH
IYRTAKEA
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]