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The map label for this gene is nuoH [H]

Identifier: 57234360

GI number: 57234360

Start: 789877

End: 790725

Strand: Direct

Name: nuoH [H]

Synonym: DET0861

Alternate gene names: 57234360

Gene position: 789877-790725 (Clockwise)

Preceding gene: 57234361

Following gene: 57234359

Centisome position: 53.74

GC content: 50.29

Gene sequence:

>849_bases
ATGAATGAATGGTTGCTGGTTGCCTTAGCTCTGGTTTTACCCCCGGTACTGGGCAGTTTGCTCCGCGGTCTTGACCGCAT
ACTGACAGCCCGAATGCAGGGCAGATTCGGCCCGCCCCTGCTTCAGCCGGTTTATGATACTATCAAACTTTTAGCTAAAC
GCCGCATGATTACCGGCCGTATGCAGGCCGTTGGTTCTATAGGTTACCTGCTGTTTATCATGGCGGCTACGGTCATGTTT
TTCCTCGGCCAGGACCTGCTGCTGATTGTGCTGGTGCTGGGGGTGGCCGATGTGTTTCTGGTTGTGGGTGCTTTTGCCAC
CCGTTCCCCGTACAGCCAGATTGGTGCTAACCGGGAACTTTTGCAAATACTCTCCTATGAACCTATCTTGATTATAACGG
CTATCGGGCTTTATGTTGCTAACGGCAGTTTCATGATTTCCGAAATAGACCAGCCCTTGCTGCCTTCTTTGTGGCCCATA
CTTTTAGCCCTGGTTCTGGTACTGGTTATTCTTATGCGTAAATCCCCGTTTGATATATCTTCTTCGGAGCATGCCCATCA
GGAACTGGTGAAGGGTGTATTTATCGAATATTCAGGTATCCACCTGGGGCTTATAGAGATTGCCCACATGTATGAGCTGA
TACTGGTGCTGGGCTTTATTTCTCTCATGTGGCTTCCGGGTATTGTGCCCGGCGTTGCCCTGGCACTGGGCTGCTGGTTT
TTGGTGCTACTGATTGACAATATTACCGCCCGCCTTACCTGGTCTTCCATGCTCAAAATCTCCTGGATGTTCGGTTTGGG
TCTGGCTTTGGTTAATCTTATCGGACTTTCAGCACTGGGAGGGGTTTAA

Upstream 100 bases:

>100_bases
CTATGTATCTTGACACCCAGTTTGCCAACCCCCATCTGGATACCGGGCTAAAAATAGCTGGCCTTTTGATACTGGTTAGT
ATGCTGGTAGTGGTGCTGAT

Downstream 100 bases:

>100_bases
GTGGATTTTGTTACTAAATCTCAAATAAAATCACCCTGGCTGCTCCATTTTGACTGCGGCAGCTGTAACGGCTGTGATAT
TGAAATACTGGCCTGTCTTA

Product: hydrogenase, EchB subunit, putative

Products: NA

Alternate protein names: NADH dehydrogenase I subunit H; NDH-1 subunit H [H]

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MNEWLLVALALVLPPVLGSLLRGLDRILTARMQGRFGPPLLQPVYDTIKLLAKRRMITGRMQAVGSIGYLLFIMAATVMF
FLGQDLLLIVLVLGVADVFLVVGAFATRSPYSQIGANRELLQILSYEPILIITAIGLYVANGSFMISEIDQPLLPSLWPI
LLALVLVLVILMRKSPFDISSSEHAHQELVKGVFIEYSGIHLGLIEIAHMYELILVLGFISLMWLPGIVPGVALALGCWF
LVLLIDNITARLTWSSMLKISWMFGLGLALVNLIGLSALGGV

Sequences:

>Translated_282_residues
MNEWLLVALALVLPPVLGSLLRGLDRILTARMQGRFGPPLLQPVYDTIKLLAKRRMITGRMQAVGSIGYLLFIMAATVMF
FLGQDLLLIVLVLGVADVFLVVGAFATRSPYSQIGANRELLQILSYEPILIITAIGLYVANGSFMISEIDQPLLPSLWPI
LLALVLVLVILMRKSPFDISSSEHAHQELVKGVFIEYSGIHLGLIEIAHMYELILVLGFISLMWLPGIVPGVALALGCWF
LVLLIDNITARLTWSSMLKISWMFGLGLALVNLIGLSALGGV
>Mature_282_residues
MNEWLLVALALVLPPVLGSLLRGLDRILTARMQGRFGPPLLQPVYDTIKLLAKRRMITGRMQAVGSIGYLLFIMAATVMF
FLGQDLLLIVLVLGVADVFLVVGAFATRSPYSQIGANRELLQILSYEPILIITAIGLYVANGSFMISEIDQPLLPSLWPI
LLALVLVLVILMRKSPFDISSSEHAHQELVKGVFIEYSGIHLGLIEIAHMYELILVLGFISLMWLPGIVPGVALALGCWF
LVLLIDNITARLTWSSMLKISWMFGLGLALVNLIGLSALGGV

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0650

COG function: function code C; Formate hydrogenlyase subunit 4

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789077, Length=297, Percent_Identity=29.6296296296296, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87082115, Length=211, Percent_Identity=30.3317535545024, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1788618, Length=300, Percent_Identity=24, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023020
- InterPro:   IPR001694
- InterPro:   IPR018086 [H]

Pfam domain/function: PF00146 NADHdh [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 30861; Mature: 30861

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEWLLVALALVLPPVLGSLLRGLDRILTARMQGRFGPPLLQPVYDTIKLLAKRRMITGR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
MQAVGSIGYLLFIMAATVMFFLGQDLLLIVLVLGVADVFLVVGAFATRSPYSQIGANREL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHH
LQILSYEPILIITAIGLYVANGSFMISEIDQPLLPSLWPILLALVLVLVILMRKSPFDIS
HHHHCCCHHHHHHHHHHHHHCCCEEHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
SSEHAHQELVKGVFIEYSGIHLGLIEIAHMYELILVLGFISLMWLPGIVPGVALALGCWF
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVLLIDNITARLTWSSMLKISWMFGLGLALVNLIGLSALGGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNEWLLVALALVLPPVLGSLLRGLDRILTARMQGRFGPPLLQPVYDTIKLLAKRRMITGR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
MQAVGSIGYLLFIMAATVMFFLGQDLLLIVLVLGVADVFLVVGAFATRSPYSQIGANREL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHH
LQILSYEPILIITAIGLYVANGSFMISEIDQPLLPSLWPILLALVLVLVILMRKSPFDIS
HHHHCCCHHHHHHHHHHHHHCCCEEHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
SSEHAHQELVKGVFIEYSGIHLGLIEIAHMYELILVLGFISLMWLPGIVPGVALALGCWF
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVLLIDNITARLTWSSMLKISWMFGLGLALVNLIGLSALGGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA