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The map label for this gene is nuoB

Identifier: 57234306

GI number: 57234306

Start: 845987

End: 846589

Strand: Direct

Name: nuoB

Synonym: DET0924

Alternate gene names: 57234306

Gene position: 845987-846589 (Clockwise)

Preceding gene: 57234307

Following gene: 57234305

Centisome position: 57.56

GC content: 52.07

Gene sequence:

>603_bases
ATGGAACTAGATTCGGGAAAACCACTGAGCCTGCTTACGCTGGATGAAATTGACCAGCATGAAGGCGGCATAATTCAAAG
CTTCCGCACCGGTCATACCACGCCTTACCCCGACCCTGCAGACTGGCTAAACAGCGAAGAGCCGCCTCCGGGTGTTTTCG
TTACCAGCGTTGAAAAGGTACTGAACTGGTCACGCCATTATTCCCTTTGGCCGGTGATGTTCGGTTTGGCCTGCTGCGCT
ATTGAAATGATGTGCATGGCAGCTTCCCGTTGGGATTTAGCCCGTTTCGGTATGGATATTTTCCGCGCCTCCCCCCGCCA
GGCAGACCTGATGATAGTTGCCGGTACTCTTACCTGGAAAATGGCTCCCTGGCTGAAAAGGATTTATGACCAGATGCCGG
AACCGAAATGGGTGCTGGCTATGGGTGCCTGCGGAACCAGCGGCGGTTTGTTCCGGGATTCATATTCGGTAGTTCCGGGT
TTCAATATGGTAGTCCCGGTTGATGTATATGTACCCGGCTGCCCTCCCCGTCCGGAAGCCCTTTTAAGGGCTATCATGGA
TATCCACGAAAAAATAGATAAAACCCGCATTATAAAGAGATAG

Upstream 100 bases:

>100_bases
AGATAGGTTCGCTCGGGTTTTATGCTTTTGGAATAGTGCTTGCCTTTCTGATAATAGTCATGGTGGGCTACCTGTATGCC
TGGAAGAAGGGGGCTTTGGA

Downstream 100 bases:

>100_bases
ATTCATGACCGCCGTACTTGATTACCAACAACTGAAGGCTGATTTATCTAAAGAACTGCCCGGGATTGAAATCCAGGCGG
AAAGTTCTTTCCTGCTGGCT

Product: proton-translocating NADH-quinone oxidoreductase, B subunit

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MELDSGKPLSLLTLDEIDQHEGGIIQSFRTGHTTPYPDPADWLNSEEPPPGVFVTSVEKVLNWSRHYSLWPVMFGLACCA
IEMMCMAASRWDLARFGMDIFRASPRQADLMIVAGTLTWKMAPWLKRIYDQMPEPKWVLAMGACGTSGGLFRDSYSVVPG
FNMVVPVDVYVPGCPPRPEALLRAIMDIHEKIDKTRIIKR

Sequences:

>Translated_200_residues
MELDSGKPLSLLTLDEIDQHEGGIIQSFRTGHTTPYPDPADWLNSEEPPPGVFVTSVEKVLNWSRHYSLWPVMFGLACCA
IEMMCMAASRWDLARFGMDIFRASPRQADLMIVAGTLTWKMAPWLKRIYDQMPEPKWVLAMGACGTSGGLFRDSYSVVPG
FNMVVPVDVYVPGCPPRPEALLRAIMDIHEKIDKTRIIKR
>Mature_200_residues
MELDSGKPLSLLTLDEIDQHEGGIIQSFRTGHTTPYPDPADWLNSEEPPPGVFVTSVEKVLNWSRHYSLWPVMFGLACCA
IEMMCMAASRWDLARFGMDIFRASPRQADLMIVAGTLTWKMAPWLKRIYDQMPEPKWVLAMGACGTSGGLFRDSYSVVPG
FNMVVPVDVYVPGCPPRPEALLRAIMDIHEKIDKTRIIKR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=146, Percent_Identity=57.5342465753425, Blast_Score=188, Evalue=3e-48,
Organism=Escherichia coli, GI1788624, Length=152, Percent_Identity=53.9473684210526, Blast_Score=181, Evalue=4e-47,
Organism=Escherichia coli, GI1789074, Length=108, Percent_Identity=42.5925925925926, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1788834, Length=110, Percent_Identity=39.0909090909091, Blast_Score=90, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17509685, Length=143, Percent_Identity=55.9440559440559, Blast_Score=179, Evalue=1e-45,
Organism=Drosophila melanogaster, GI18859983, Length=146, Percent_Identity=53.4246575342466, Blast_Score=184, Evalue=5e-47,
Organism=Drosophila melanogaster, GI24642371, Length=146, Percent_Identity=53.4246575342466, Blast_Score=184, Evalue=5e-47,
Organism=Drosophila melanogaster, GI24651058, Length=143, Percent_Identity=57.3426573426573, Blast_Score=182, Evalue=1e-46,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB_DEHE1 (Q3Z800)

Other databases:

- EMBL:   CP000027
- RefSeq:   YP_181649.1
- HSSP:   Q56218
- ProteinModelPortal:   Q3Z800
- SMR:   Q3Z800
- STRING:   Q3Z800
- GeneID:   3229773
- GenomeReviews:   CP000027_GR
- KEGG:   det:DET0924
- NMPDR:   fig|243164.3.peg.1223
- TIGR:   DET0924
- eggNOG:   COG0377
- HOGENOM:   HBG553221
- OMA:   CCAIEFM
- PhylomeDB:   Q3Z800
- ProtClustDB:   CLSK837205
- BioCyc:   DETH243164:DET_0924-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 22515; Mature: 22515

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
8.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
6.0 %Met     (Mature Protein)
8.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELDSGKPLSLLTLDEIDQHEGGIIQSFRTGHTTPYPDPADWLNSEEPPPGVFVTSVEKV
CCCCCCCCEEEEEHHHCCCCCCCHHHHHHCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHH
LNWSRHYSLWPVMFGLACCAIEMMCMAASRWDLARFGMDIFRASPRQADLMIVAGTLTWK
HHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEECCHHHH
MAPWLKRIYDQMPEPKWVLAMGACGTSGGLFRDSYSVVPGFNMVVPVDVYVPGCPPRPEA
HHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCHHH
LLRAIMDIHEKIDKTRIIKR
HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MELDSGKPLSLLTLDEIDQHEGGIIQSFRTGHTTPYPDPADWLNSEEPPPGVFVTSVEKV
CCCCCCCCEEEEEHHHCCCCCCCHHHHHHCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHH
LNWSRHYSLWPVMFGLACCAIEMMCMAASRWDLARFGMDIFRASPRQADLMIVAGTLTWK
HHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEECCHHHH
MAPWLKRIYDQMPEPKWVLAMGACGTSGGLFRDSYSVVPGFNMVVPVDVYVPGCPPRPEA
HHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCHHH
LLRAIMDIHEKIDKTRIIKR
HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA