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The map label for this gene is gspE [H]

Identifier: 57233906

GI number: 57233906

Start: 1220622

End: 1222958

Strand: Direct

Name: gspE [H]

Synonym: DET1351

Alternate gene names: 57233906

Gene position: 1220622-1222958 (Clockwise)

Preceding gene: 57233907

Following gene: 57233905

Centisome position: 83.05

GC content: 49.42

Gene sequence:

>2337_bases
ATGTTGCAGAACAAAGTTAAAGATACAGAACTCCGCAAACTGGTTAGTAAACGGGTGGCAGATTATCTGTCCAGCCACAG
CTACCAGGTGGAAACTGATGTCAAAATAACCGGTCAGTCCGGTGTGGAGCACGTCTTTGATATAGTAGCCCGCCGGAATG
ACGGTTTTACTACCAGAACAATGGCGGTTTGCATAACTCTGGGTTCAAACCAGGAAGAAGAAAGCAGCGCTATTTTTAAC
TTTGCCAATAAGGCTTTTGACACCGGTATAAATGAACGTATTCTTATTGCCATACCCAAACTCACCCCCGAAACCCGCAA
TCTGGCTGAAAAACAGCGAATAAAAGTCTTTGATGAAGACCGTCTGGAAGACCTGCTGGTCAGCAATACCAAACACCCCA
GCCGTCTGGAGAGTATAGGCAACATCTCCACCCGCGAAGACCTTATTAACGCTCTGACCGGCCTGGGCTATACCATAAGG
GAAAATGCCCGTATCAAAGGGCGTTCCGGCATTTCACACGTGTTTGACATAGTGGCTAATGATATCAATACTGACAACTA
TACCCTGGGGATAGACATTATCAAAAAACAGCCCTCCCTTGAGCTTCAGGATGTGGCTCTGTTTGACGCTAAAGCCTTTG
ATTGCGGCATAGTTGACAAGCTGATAGCTACCGCCTCCCAGATAACCGCTGATGCCCAGCAGTTTGCTGATGCCCAGAAG
GTCAAGCTTATAAAATTTAAACCCGTTACTATAGCTACCGAAGAGGGGGCTGCGGTAAATACAGCCCAAGAAGTTATAAA
GACCTCAGCCAAAACCGACACTAAAAACGGCAAAACTGCCATAACCGACCTGCGCCAGTCACCCCGCCCCGAAGCGCTCA
AGCTCATTCCCGAAGTGCTTGCCCGGCGTTACACTGCTATACCCCTGAATGTAGTGGGTAATACCCTTGAGATGGCCATG
GCAGACCCGTCTGACATACTGGCACTTGAAGCCTTTTCCGCCCATTCCAAACGCCGCATCAAGCCCATACCGGCAGATGC
CCGCGAAATACGCGAAGCTATAGACTTTAACTACAAAGGTTACGGGGATATTGAAAAATACCTTTCCCGCATGTCAATAC
CCAGCGAAGTCACCGATGACCGTCTGGCTATTGATGCCGCTATTGACGCACCTTTAGCCCAAGCCCTGAACCTGATTATT
GAAGAAGCGGTAAAAGCCCGCTCGTCAGACGTACATATTGAGCCCAACGAAGACCGTCTGCGGGTGCGTTTCCGTATTGA
CGGCACACTTCAGGATATGATGTCTTTACCCATCACCGCCCACCGGGCTATTATTTCCAGAATAAAAATCCTGGGTGATA
TGAATATTGCAGACCATTTCCACGCACTTGACGGCCAGTTTACGGTAAATGCCGGCGGACGGGAAATAGATATCCGGGCG
GCAACCGCTCCTACCGTAAACGGGGAAATGTCGGTGCTGCGGCTGCTGGATAAAAGCCGGGCTACCATAGAACTTTCCCA
GCTGGGCTTTTTGCCGGACAGCCTGGAAAAATACAACAAAATGCTCAAAGTGCCCTACGGCATGATACTTATCAGCGGCC
CTACCGGCGCCGGTAAGACCACTACCCTTTATGCATCTATAAACTCGCTGGATATCATGCGGCAAAATATTATTACCATA
GAAGACCCGGCGGAATACCGCTTTAAAAATATAAACCAGATACAGGTAAATACCCAGGCCGGCATTACCTTTGCCAGCGG
CCTGCGTTCTATCCTCCGCCTTGACCCTGATGTAATCCTGGTAGGTGAAATACGTGATGCTGAAACAGCCAATATAGCCG
TTCAGGCAGCCCTTACCGGCCACCTGATGCTTTCATCTATACACGCCAATGACACTACCGGCGTCCTTTACCGCTTGATA
GACCTGGGAGTAGAACCCTTCCTGATTGCTTCAGCTGTGGTAGGGGTAGTAGCCCAGCGTATGGTCAGGCGGATTTGCCC
GTACTGCCAGCATACCATTGAAGTACCGGTTATTGAGCAGGTAGCTTACGAACGGGAAATCGGGGAGAAACGAACCAAAT
TTGTATATAGCAGCGGCTGTAAATCATGTGCCTTCACCGGCTATCTGGGCCGGGTGGGCCTGTTTGAGATAATGACTATA
AGTGATGAAATCAGGCGGCTGATGCTAAGCGGGGCTTCCCCGTCCGAAATACACGCCCAATCCATTAAAGACGGCATGAT
TACCATGATGAAAGATGGTATGCTGAAGGTAAAAGACAATATAACCACCCCCTCCGAAGTACTACGCAGTGCCTATTCTC
CGGAGGAGCAGTATTGA

Upstream 100 bases:

>100_bases
CTTATTTTTGACAAATAGTTGCACCTTTAGGTGAATTATGCGTAAGATATGATAAGTAACTGGATAAAAAGCCAATAAAC
AAGCTGGAATGTAGTAAGAA

Downstream 100 bases:

>100_bases
GGTAAGTAGATGGACTTTAATTACGTAGCTTACGGTCAGGACAAAAGGCTTGTAAAAGGGAAAATCCCGGCGACCTCTCT
GGAGGCAGCCCAGAGGCTGC

Product: type II secretion system protein GspE

Products: NA

Alternate protein names: Pullulanase secretion protein pulE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 778; Mature: 778

Protein sequence:

>778_residues
MLQNKVKDTELRKLVSKRVADYLSSHSYQVETDVKITGQSGVEHVFDIVARRNDGFTTRTMAVCITLGSNQEEESSAIFN
FANKAFDTGINERILIAIPKLTPETRNLAEKQRIKVFDEDRLEDLLVSNTKHPSRLESIGNISTREDLINALTGLGYTIR
ENARIKGRSGISHVFDIVANDINTDNYTLGIDIIKKQPSLELQDVALFDAKAFDCGIVDKLIATASQITADAQQFADAQK
VKLIKFKPVTIATEEGAAVNTAQEVIKTSAKTDTKNGKTAITDLRQSPRPEALKLIPEVLARRYTAIPLNVVGNTLEMAM
ADPSDILALEAFSAHSKRRIKPIPADAREIREAIDFNYKGYGDIEKYLSRMSIPSEVTDDRLAIDAAIDAPLAQALNLII
EEAVKARSSDVHIEPNEDRLRVRFRIDGTLQDMMSLPITAHRAIISRIKILGDMNIADHFHALDGQFTVNAGGREIDIRA
ATAPTVNGEMSVLRLLDKSRATIELSQLGFLPDSLEKYNKMLKVPYGMILISGPTGAGKTTTLYASINSLDIMRQNIITI
EDPAEYRFKNINQIQVNTQAGITFASGLRSILRLDPDVILVGEIRDAETANIAVQAALTGHLMLSSIHANDTTGVLYRLI
DLGVEPFLIASAVVGVVAQRMVRRICPYCQHTIEVPVIEQVAYEREIGEKRTKFVYSSGCKSCAFTGYLGRVGLFEIMTI
SDEIRRLMLSGASPSEIHAQSIKDGMITMMKDGMLKVKDNITTPSEVLRSAYSPEEQY

Sequences:

>Translated_778_residues
MLQNKVKDTELRKLVSKRVADYLSSHSYQVETDVKITGQSGVEHVFDIVARRNDGFTTRTMAVCITLGSNQEEESSAIFN
FANKAFDTGINERILIAIPKLTPETRNLAEKQRIKVFDEDRLEDLLVSNTKHPSRLESIGNISTREDLINALTGLGYTIR
ENARIKGRSGISHVFDIVANDINTDNYTLGIDIIKKQPSLELQDVALFDAKAFDCGIVDKLIATASQITADAQQFADAQK
VKLIKFKPVTIATEEGAAVNTAQEVIKTSAKTDTKNGKTAITDLRQSPRPEALKLIPEVLARRYTAIPLNVVGNTLEMAM
ADPSDILALEAFSAHSKRRIKPIPADAREIREAIDFNYKGYGDIEKYLSRMSIPSEVTDDRLAIDAAIDAPLAQALNLII
EEAVKARSSDVHIEPNEDRLRVRFRIDGTLQDMMSLPITAHRAIISRIKILGDMNIADHFHALDGQFTVNAGGREIDIRA
ATAPTVNGEMSVLRLLDKSRATIELSQLGFLPDSLEKYNKMLKVPYGMILISGPTGAGKTTTLYASINSLDIMRQNIITI
EDPAEYRFKNINQIQVNTQAGITFASGLRSILRLDPDVILVGEIRDAETANIAVQAALTGHLMLSSIHANDTTGVLYRLI
DLGVEPFLIASAVVGVVAQRMVRRICPYCQHTIEVPVIEQVAYEREIGEKRTKFVYSSGCKSCAFTGYLGRVGLFEIMTI
SDEIRRLMLSGASPSEIHAQSIKDGMITMMKDGMLKVKDNITTPSEVLRSAYSPEEQY
>Mature_778_residues
MLQNKVKDTELRKLVSKRVADYLSSHSYQVETDVKITGQSGVEHVFDIVARRNDGFTTRTMAVCITLGSNQEEESSAIFN
FANKAFDTGINERILIAIPKLTPETRNLAEKQRIKVFDEDRLEDLLVSNTKHPSRLESIGNISTREDLINALTGLGYTIR
ENARIKGRSGISHVFDIVANDINTDNYTLGIDIIKKQPSLELQDVALFDAKAFDCGIVDKLIATASQITADAQQFADAQK
VKLIKFKPVTIATEEGAAVNTAQEVIKTSAKTDTKNGKTAITDLRQSPRPEALKLIPEVLARRYTAIPLNVVGNTLEMAM
ADPSDILALEAFSAHSKRRIKPIPADAREIREAIDFNYKGYGDIEKYLSRMSIPSEVTDDRLAIDAAIDAPLAQALNLII
EEAVKARSSDVHIEPNEDRLRVRFRIDGTLQDMMSLPITAHRAIISRIKILGDMNIADHFHALDGQFTVNAGGREIDIRA
ATAPTVNGEMSVLRLLDKSRATIELSQLGFLPDSLEKYNKMLKVPYGMILISGPTGAGKTTTLYASINSLDIMRQNIITI
EDPAEYRFKNINQIQVNTQAGITFASGLRSILRLDPDVILVGEIRDAETANIAVQAALTGHLMLSSIHANDTTGVLYRLI
DLGVEPFLIASAVVGVVAQRMVRRICPYCQHTIEVPVIEQVAYEREIGEKRTKFVYSSGCKSCAFTGYLGRVGLFEIMTI
SDEIRRLMLSGASPSEIHAQSIKDGMITMMKDGMLKVKDNITTPSEVLRSAYSPEEQY

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of pullulanase [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=412, Percent_Identity=40.2912621359223, Blast_Score=317, Evalue=2e-87,
Organism=Escherichia coli, GI1786296, Length=434, Percent_Identity=35.7142857142857, Blast_Score=298, Evalue=9e-82,
Organism=Escherichia coli, GI87082188, Length=172, Percent_Identity=40.1162790697674, Blast_Score=108, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 85957; Mature: 85957

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQNKVKDTELRKLVSKRVADYLSSHSYQVETDVKITGQSGVEHVFDIVARRNDGFTTRT
CCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHCCCCCCEEE
MAVCITLGSNQEEESSAIFNFANKAFDTGINERILIAIPKLTPETRNLAEKQRIKVFDED
EEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCHH
RLEDLLVSNTKHPSRLESIGNISTREDLINALTGLGYTIRENARIKGRSGISHVFDIVAN
HHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHCCEEEECCCEECCCCCHHHHHHHHHC
DINTDNYTLGIDIIKKQPSLELQDVALFDAKAFDCGIVDKLIATASQITADAQQFADAQK
CCCCCCEEEEEEEEECCCCCCCHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHH
VKLIKFKPVTIATEEGAAVNTAQEVIKTSAKTDTKNGKTAITDLRQSPRPEALKLIPEVL
EEEEEEECEEEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHH
ARRYTAIPLNVVGNTLEMAMADPSDILALEAFSAHSKRRIKPIPADAREIREAIDFNYKG
HHHHCCCCHHHHCCHHHHHCCCCHHHEEEHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCC
YGDIEKYLSRMSIPSEVTDDRLAIDAAIDAPLAQALNLIIEEAVKARSSDVHIEPNEDRL
CCHHHHHHHHCCCCCCCCCCCEEEEHHHCHHHHHHHHHHHHHHHHCCCCCEEECCCCCEE
RVRFRIDGTLQDMMSLPITAHRAIISRIKILGDMNIADHFHALDGQFTVNAGGREIDIRA
EEEEEECCCHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEEECCCCEEEEEE
ATAPTVNGEMSVLRLLDKSRATIELSQLGFLPDSLEKYNKMLKVPYGMILISGPTGAGKT
ECCCCCCCHHHHHHHHHCCCCEEEHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCC
TTLYASINSLDIMRQNIITIEDPAEYRFKNINQIQVNTQAGITFASGLRSILRLDPDVIL
EEEEEECCHHHHHHHCCEEEECCHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEE
VGEIRDAETANIAVQAALTGHLMLSSIHANDTTGVLYRLIDLGVEPFLIASAVVGVVAQR
EECCCCCCCCCEEEEEEHHHHHHEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
MVRRICPYCQHTIEVPVIEQVAYEREIGEKRTKFVYSSGCKSCAFTGYLGRVGLFEIMTI
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHH
SDEIRRLMLSGASPSEIHAQSIKDGMITMMKDGMLKVKDNITTPSEVLRSAYSPEEQY
HHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHCCCEEEECCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLQNKVKDTELRKLVSKRVADYLSSHSYQVETDVKITGQSGVEHVFDIVARRNDGFTTRT
CCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHCCCCCCEEE
MAVCITLGSNQEEESSAIFNFANKAFDTGINERILIAIPKLTPETRNLAEKQRIKVFDED
EEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCHH
RLEDLLVSNTKHPSRLESIGNISTREDLINALTGLGYTIRENARIKGRSGISHVFDIVAN
HHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHCCEEEECCCEECCCCCHHHHHHHHHC
DINTDNYTLGIDIIKKQPSLELQDVALFDAKAFDCGIVDKLIATASQITADAQQFADAQK
CCCCCCEEEEEEEEECCCCCCCHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHH
VKLIKFKPVTIATEEGAAVNTAQEVIKTSAKTDTKNGKTAITDLRQSPRPEALKLIPEVL
EEEEEEECEEEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHH
ARRYTAIPLNVVGNTLEMAMADPSDILALEAFSAHSKRRIKPIPADAREIREAIDFNYKG
HHHHCCCCHHHHCCHHHHHCCCCHHHEEEHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCC
YGDIEKYLSRMSIPSEVTDDRLAIDAAIDAPLAQALNLIIEEAVKARSSDVHIEPNEDRL
CCHHHHHHHHCCCCCCCCCCCEEEEHHHCHHHHHHHHHHHHHHHHCCCCCEEECCCCCEE
RVRFRIDGTLQDMMSLPITAHRAIISRIKILGDMNIADHFHALDGQFTVNAGGREIDIRA
EEEEEECCCHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEEECCCCEEEEEE
ATAPTVNGEMSVLRLLDKSRATIELSQLGFLPDSLEKYNKMLKVPYGMILISGPTGAGKT
ECCCCCCCHHHHHHHHHCCCCEEEHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCC
TTLYASINSLDIMRQNIITIEDPAEYRFKNINQIQVNTQAGITFASGLRSILRLDPDVIL
EEEEEECCHHHHHHHCCEEEECCHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEE
VGEIRDAETANIAVQAALTGHLMLSSIHANDTTGVLYRLIDLGVEPFLIASAVVGVVAQR
EECCCCCCCCCEEEEEEHHHHHHEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
MVRRICPYCQHTIEVPVIEQVAYEREIGEKRTKFVYSSGCKSCAFTGYLGRVGLFEIMTI
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHH
SDEIRRLMLSGASPSEIHAQSIKDGMITMMKDGMLKVKDNITTPSEVLRSAYSPEEQY
HHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHCCCEEEECCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1738317; 2677007 [H]