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The map label for this gene is pilT-3 [H]

Identifier: 57233890

GI number: 57233890

Start: 1229887

End: 1231002

Strand: Direct

Name: pilT-3 [H]

Synonym: DET1359

Alternate gene names: 57233890

Gene position: 1229887-1231002 (Clockwise)

Preceding gene: 57233891

Following gene: 57233881

Centisome position: 83.68

GC content: 54.75

Gene sequence:

>1116_bases
ATGGACGTTATAAAACTTATCTACGAAGCCGAAGAAAAAAAAGGCTCTGACCTGCATCTGGTAGCCCAAAGCCCGCCGCT
GGTGCGGGTAAGAGGGGATTTGGAAGCCCTGAACTCTTATGAACAGCTGCAGCCGGCAGATATCAAAACCGCTTTTGAAC
AGCTGACTACCCATGAAGAACGGGAAACTTTTTACAAGGAATGGGAGCTGGATTTCGGCTACTCACTGGAGGGAGTGGGA
CGCCTAAGGTGCAATGCCGCCATGCAAAGGGGAGCCATAAGTCTGGCTATCCGCCTGCTGCCGCCCAGCATACCCACTAT
CAGCGAGCTGGAATTACCGGATATTTGTGAAAAACTGATTACCAAACCCCGCGGCTTGGTGATTGTAACCGGACCTACCG
GCAGCGGCAAGAGCACCACCCTGGCGGCTATGATACAGCACCTGAACAACACCGAAAACAAGCACGTGGTCACTATTGAA
GACCCGATTGAATACGTTCACCCCTGTATAAAATGCGCCGTCACCCAGCGCCAGCTTGGCTCAGACACCCTTTCCTTCGG
CCATGCCCTGAAACACGTACTCCGCCAGAACCCTGACGTAATCATGGTGGGTGAAATGCGTGACCTGGATACGGCCGCGG
CAGTGCTGACTATTGCCGAAACAGGCCATCTGGTGCTCTCCACCTCCCATGCACCCAGCGCCTATCAGGCCCTGGAGCGG
ATTATAGACCTGTTCCCGCCCCATGAAAGGCATCTGGCCCAGACCCGTCTGGCCTCGCTGATTGAGGGCGTTCTCTGCCA
GACCTTAGTACCCCGTGCCAATAGCGACGGGCGGGTGGTGGCAGTGGAAATAATGCTGGGCAGCCCGGCGGTCAGAAACC
TTATCCGCGAGGGTAAGTTTTACCAGCTGCCGAATGTTATCCGCACCAGCCGGGATGAGGGCATGATAACCATGGACGAG
GCTTTAGTGGAACTTTACCGCCAGCAGGTAATCAGCCGGGAAGACCTGTTTAACTACTGTCAGGACGCATCCGAGGTGGA
AAAGCTGATAAGCAGCAGCTCCGCCAGCCAGAAACGCAAAAAGCTGGTGGAAAGCAGCAAGAGCCTGCTTTTTTAA

Upstream 100 bases:

>100_bases
GATGTAGTGGTAATCAGCATGGACAAGATACTGACCGAAGACCCGAATATGCCTATATACAGTTTTAATTTCCTTTTGAA
ATAAACTAGTCTGGTAAAAC

Downstream 100 bases:

>100_bases
AGAAGCTGCTACTAAAAACAGATTAAGGGCGGTCTAAACTAACCGCCCTTTTTGTTTGGCCAAACAAGGTTACGAAATAT
AATAAAGGTCTGCTTTAGGG

Product: twitching mobility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MDVIKLIYEAEEKKGSDLHLVAQSPPLVRVRGDLEALNSYEQLQPADIKTAFEQLTTHEERETFYKEWELDFGYSLEGVG
RLRCNAAMQRGAISLAIRLLPPSIPTISELELPDICEKLITKPRGLVIVTGPTGSGKSTTLAAMIQHLNNTENKHVVTIE
DPIEYVHPCIKCAVTQRQLGSDTLSFGHALKHVLRQNPDVIMVGEMRDLDTAAAVLTIAETGHLVLSTSHAPSAYQALER
IIDLFPPHERHLAQTRLASLIEGVLCQTLVPRANSDGRVVAVEIMLGSPAVRNLIREGKFYQLPNVIRTSRDEGMITMDE
ALVELYRQQVISREDLFNYCQDASEVEKLISSSSASQKRKKLVESSKSLLF

Sequences:

>Translated_371_residues
MDVIKLIYEAEEKKGSDLHLVAQSPPLVRVRGDLEALNSYEQLQPADIKTAFEQLTTHEERETFYKEWELDFGYSLEGVG
RLRCNAAMQRGAISLAIRLLPPSIPTISELELPDICEKLITKPRGLVIVTGPTGSGKSTTLAAMIQHLNNTENKHVVTIE
DPIEYVHPCIKCAVTQRQLGSDTLSFGHALKHVLRQNPDVIMVGEMRDLDTAAAVLTIAETGHLVLSTSHAPSAYQALER
IIDLFPPHERHLAQTRLASLIEGVLCQTLVPRANSDGRVVAVEIMLGSPAVRNLIREGKFYQLPNVIRTSRDEGMITMDE
ALVELYRQQVISREDLFNYCQDASEVEKLISSSSASQKRKKLVESSKSLLF
>Mature_371_residues
MDVIKLIYEAEEKKGSDLHLVAQSPPLVRVRGDLEALNSYEQLQPADIKTAFEQLTTHEERETFYKEWELDFGYSLEGVG
RLRCNAAMQRGAISLAIRLLPPSIPTISELELPDICEKLITKPRGLVIVTGPTGSGKSTTLAAMIQHLNNTENKHVVTIE
DPIEYVHPCIKCAVTQRQLGSDTLSFGHALKHVLRQNPDVIMVGEMRDLDTAAAVLTIAETGHLVLSTSHAPSAYQALER
IIDLFPPHERHLAQTRLASLIEGVLCQTLVPRANSDGRVVAVEIMLGSPAVRNLIREGKFYQLPNVIRTSRDEGMITMDE
ALVELYRQQVISREDLFNYCQDASEVEKLISSSSASQKRKKLVESSKSLLF

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=306, Percent_Identity=42.483660130719, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI1789723, Length=157, Percent_Identity=44.5859872611465, Blast_Score=117, Evalue=8e-28,
Organism=Escherichia coli, GI1786296, Length=161, Percent_Identity=41.6149068322981, Blast_Score=101, Evalue=9e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 41289; Mature: 41289

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVIKLIYEAEEKKGSDLHLVAQSPPLVRVRGDLEALNSYEQLQPADIKTAFEQLTTHEE
CHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
RETFYKEWELDFGYSLEGVGRLRCNAAMQRGAISLAIRLLPPSIPTISELELPDICEKLI
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCHHEEEEEECCCCCCCCCCCCCHHHHHHHH
TKPRGLVIVTGPTGSGKSTTLAAMIQHLNNTENKHVVTIEDPIEYVHPCIKCAVTQRQLG
CCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHC
SDTLSFGHALKHVLRQNPDVIMVGEMRDLDTAAAVLTIAETGHLVLSTSHAPSAYQALER
CHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHEEECCCCEEEEECCCCHHHHHHHH
IIDLFPPHERHLAQTRLASLIEGVLCQTLVPRANSDGRVVAVEIMLGSPAVRNLIREGKF
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCHHHHHHHHCCCC
YQLPNVIRTSRDEGMITMDEALVELYRQQVISREDLFNYCQDASEVEKLISSSSASQKRK
EECCHHHHCCCCCCCEEHHHHHHHHHHHHHHCHHHHHHHHCCHHHHHHHHHCCCHHHHHH
KLVESSKSLLF
HHHHHHHHCCC
>Mature Secondary Structure
MDVIKLIYEAEEKKGSDLHLVAQSPPLVRVRGDLEALNSYEQLQPADIKTAFEQLTTHEE
CHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
RETFYKEWELDFGYSLEGVGRLRCNAAMQRGAISLAIRLLPPSIPTISELELPDICEKLI
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCHHEEEEEECCCCCCCCCCCCCHHHHHHHH
TKPRGLVIVTGPTGSGKSTTLAAMIQHLNNTENKHVVTIEDPIEYVHPCIKCAVTQRQLG
CCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHC
SDTLSFGHALKHVLRQNPDVIMVGEMRDLDTAAAVLTIAETGHLVLSTSHAPSAYQALER
CHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHEEECCCCEEEEECCCCHHHHHHHH
IIDLFPPHERHLAQTRLASLIEGVLCQTLVPRANSDGRVVAVEIMLGSPAVRNLIREGKF
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCHHHHHHHHCCCC
YQLPNVIRTSRDEGMITMDEALVELYRQQVISREDLFNYCQDASEVEKLISSSSASQKRK
EECCHHHHCCCCCCCEEHHHHHHHHHHHHHHCHHHHHHHHCCHHHHHHHHHCCCHHHHHH
KLVESSKSLLF
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]