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The map label for this gene is trpA

Identifier: 57233819

GI number: 57233819

Start: 1345894

End: 1346661

Strand: Direct

Name: trpA

Synonym: DET1488

Alternate gene names: 57233819

Gene position: 1345894-1346661 (Clockwise)

Preceding gene: 57233820

Following gene: 57233818

Centisome position: 91.57

GC content: 48.7

Gene sequence:

>768_bases
ATGAGCCGTATCAGTGATGCATTCCAAAAACGAAAATCTCTTATAGCTTACATAACAGTCGGCTACCCGGATATTGAAAC
CACCCTGCGGCTGGTGCCTCTGCTGGAAGAAAACGGGGTGGATATTATAGAGCTGGGTATTCCCTTCTCAGACCCGCTGG
CAGACGGAGTTACTATTCAAAATGCCAGTTATCAGGCACTCCAGAACGGGGTGACGCCTGAAGTCTGTTTATCTGTTGCC
GCCCTGCTCAAAGAAAAAATCTCCATACCCATGGTATTCATGGGCTATTATAATCCCATTTACAACTACGGACTGACCAA
GTTCTGCCAAAAGTGTGCAACTGCGGGTGTAAGCGGATTTATTATTCCTGACCTGCCGCCGGGAGAAGCCCAAGACATAG
ACTTTGCCGCCACAGAAGCCGGCTTGGATATAATCTTTCTGTTAGCCCCCACCAGTACCGACGAACGGATTAAGCTAGTT
GCCGCCAAATCACGGGGGTTTATTTATCTCGTTTCCCACTCAGGCGTTACCGGGGCAACTGCCAACTTGCCGGCTGATTT
GAGCAGTTTTGTAAACAGGGTAAGGAAAACCGCCCGCCAGCCTCTGGCAGTGGGCTTTGGTATTTCAACCCCCGAACAAG
CCCAAAATATCGCAAAATTTTCCGACGGGATAATAGTAGGCAGCCGTATCCTGCAGCTTGTCCAGACAGACCCTTCACTT
GAAAAAGTAGCCACATTTATCAGGCAACTCAGACAATCATTAGACTAA

Upstream 100 bases:

>100_bases
TAGCAGTCGGCATGCCGAAAAACAAAAATATAGTCATAAACCTCTCCGGCAGAGGGGACAAAGACATGGATATTGTTCAA
AAAGCAATGGGGGTAAAGAT

Downstream 100 bases:

>100_bases
CCGAAAAATCTGCTTTATGCTGAATAATTTCGGCTACTAATCTATAAAAACCGGGGGATAGCAACAGGGGAGGTTAACCT
CCCCGCTGTACTTAGTAATA

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MSRISDAFQKRKSLIAYITVGYPDIETTLRLVPLLEENGVDIIELGIPFSDPLADGVTIQNASYQALQNGVTPEVCLSVA
ALLKEKISIPMVFMGYYNPIYNYGLTKFCQKCATAGVSGFIIPDLPPGEAQDIDFAATEAGLDIIFLLAPTSTDERIKLV
AAKSRGFIYLVSHSGVTGATANLPADLSSFVNRVRKTARQPLAVGFGISTPEQAQNIAKFSDGIIVGSRILQLVQTDPSL
EKVATFIRQLRQSLD

Sequences:

>Translated_255_residues
MSRISDAFQKRKSLIAYITVGYPDIETTLRLVPLLEENGVDIIELGIPFSDPLADGVTIQNASYQALQNGVTPEVCLSVA
ALLKEKISIPMVFMGYYNPIYNYGLTKFCQKCATAGVSGFIIPDLPPGEAQDIDFAATEAGLDIIFLLAPTSTDERIKLV
AAKSRGFIYLVSHSGVTGATANLPADLSSFVNRVRKTARQPLAVGFGISTPEQAQNIAKFSDGIIVGSRILQLVQTDPSL
EKVATFIRQLRQSLD
>Mature_254_residues
SRISDAFQKRKSLIAYITVGYPDIETTLRLVPLLEENGVDIIELGIPFSDPLADGVTIQNASYQALQNGVTPEVCLSVAA
LLKEKISIPMVFMGYYNPIYNYGLTKFCQKCATAGVSGFIIPDLPPGEAQDIDFAATEAGLDIIFLLAPTSTDERIKLVA
AKSRGFIYLVSHSGVTGATANLPADLSSFVNRVRKTARQPLAVGFGISTPEQAQNIAKFSDGIIVGSRILQLVQTDPSLE
KVATFIRQLRQSLD

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=208, Percent_Identity=37.5, Blast_Score=152, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6321412, Length=232, Percent_Identity=45.2586206896552, Blast_Score=215, Evalue=5e-57,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_DEHE1 (Q3Z6G1)

Other databases:

- EMBL:   CP000027
- RefSeq:   YP_182188.1
- HSSP:   P42390
- ProteinModelPortal:   Q3Z6G1
- SMR:   Q3Z6G1
- STRING:   Q3Z6G1
- GeneID:   3229282
- GenomeReviews:   CP000027_GR
- KEGG:   det:DET1488
- TIGR:   DET1488
- eggNOG:   COG0159
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- PhylomeDB:   Q3Z6G1
- ProtClustDB:   PRK13111
- BioCyc:   DETH243164:DET_1488-MONOMER
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 27572; Mature: 27441

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 44-44 ACT_SITE 55-55

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRISDAFQKRKSLIAYITVGYPDIETTLRLVPLLEENGVDIIELGIPFSDPLADGVTIQ
CCHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEC
NASYQALQNGVTPEVCLSVAALLKEKISIPMVFMGYYNPIYNYGLTKFCQKCATAGVSGF
CCHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCHHHHHHHHHHCCCCCE
IIPDLPPGEAQDIDFAATEAGLDIIFLLAPTSTDERIKLVAAKSRGFIYLVSHSGVTGAT
ECCCCCCCCCCCCCHHHHHCCCEEEEEEECCCCCCEEEEEEECCCCEEEEEECCCCCCCC
ANLPADLSSFVNRVRKTARQPLAVGFGISTPEQAQNIAKFSDGIIVGSRILQLVQTDPSL
CCCCHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCH
EKVATFIRQLRQSLD
HHHHHHHHHHHHHCC
>Mature Secondary Structure 
SRISDAFQKRKSLIAYITVGYPDIETTLRLVPLLEENGVDIIELGIPFSDPLADGVTIQ
CHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEC
NASYQALQNGVTPEVCLSVAALLKEKISIPMVFMGYYNPIYNYGLTKFCQKCATAGVSGF
CCHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCHHHHHHHHHHCCCCCE
IIPDLPPGEAQDIDFAATEAGLDIIFLLAPTSTDERIKLVAAKSRGFIYLVSHSGVTGAT
ECCCCCCCCCCCCCHHHHHCCCEEEEEEECCCCCCEEEEEEECCCCEEEEEECCCCCCCC
ANLPADLSSFVNRVRKTARQPLAVGFGISTPEQAQNIAKFSDGIIVGSRILQLVQTDPSL
CCCCHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCH
EKVATFIRQLRQSLD
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA