Click here to switch to the map view.

The map label for this gene is aspA [H]

Identifier: 56963497

GI number: 56963497

Start: 1859467

End: 1860879

Strand: Direct

Name: aspA [H]

Synonym: ABC1732

Alternate gene names: 56963497

Gene position: 1859467-1860879 (Clockwise)

Preceding gene: 56963496

Following gene: 56963498

Centisome position: 43.2

GC content: 46.43

Gene sequence:

>1413_bases
ATGGAGTACCGGGTTGAACGAGACTTACTAGGAGAAAAACAAGTACCGAAACATGCTTATTATGGCATCCAATCGCTGCG
GGCAAAGGATAATTTTCCGATTACCGGCTATCCGCCCCACCCTGAACTTATTCGCGCTTTCGGCTATGTGAAAAAAGCGG
CTGCCCTTGCAAACCGGGATGTCGGTGTACTGCGCCCCCACATTGCTGAAGCGATTATTAAGGCAAGCGATGAAGTAATT
GAAGGGCGTTTGAATGATCATTTTATTGTCGATTCGATCCAAGGAGGCGCCGGCACGTCGTTTAATATGAATGCCAATGA
AGTCATTGCTAACCGGGCGATTGAAATTCTTGGCGGCGAAAAAGGCGATTATTTAAAAGTCAGTCCCAACACACACGTCA
ACATGGCCCAGTCTACCAATGATTCATTTCCGACGGCGATTCATATTGCCGCTTTAAATATGACAAAGGGCTTGACGGAA
GAATTGCAGAAATTGATCAACGAAATGGAAGCGAAAGCCGAAGAATTTGACAGCGTCCTAAAAATGGGGCGGACCCACTT
GCAAGATGCAGTCCCTATTCGACTAGGGCAGGAATTTGGCTCGTACCGCAGAGTGCTAGCCCGTGATCTAAAACGGGTAA
GCCGTTCGGCAGACCACCTCCACGACATCAATATGGGTGCAACCGCTGTAGGAACGGGTTTGAACGCCAAACCGGAATAC
ATTGAAAAAGTTGCAAAACATTTGGCTGACTTGACTGAGCTGCCGTTGAAAACGGCGGAGGATCTTGTCGATGCCACCCA
AAATACAGATGCCTATACAGAGCTTTCTAGTTCGTTAAAAATTTTAGCTATTAATTTATCGAAAATCGCAAATGACTTAC
GTTTAATGAGCTCGGGCCCGCGGACCGGATTGAATGAAATCAATTTGCCGCCGCGACAGCCGGGTTCATCGATCATGCCT
GGAAAAGTAAATCCGGTTATGTGTGAAGTCATTAACCAGCTTTCTTTTCAAGTTATCGGCAATGACCATACGATCAGCCT
TGCTTCAGAAGCGGGCCAACTTGAACTAAACGTGATGGAACCTGTACTCGTTTTTAATTTATTGCAATCATTAACGGTGC
TACAAAACGGTATGCGCGTCTTTAAAGATTACGCAATTGCCGGCATTACCGCTAACGTGGAGCGATGCCGGGAACTTGTA
GAACATAGCGTCGGTATTGTAACGGCAATCAATCCCCATGTCGGCTATGAAGTAGCTACTCGTGTAGCTAAAGAAGCAAT
TGAAACAGGGCGGCCGGTCCGGGAAATTTGTCTTGAACGGGGCATCCTTTCAGAGGAAGAATTGAATGAAATTCTTGATC
CAAAAGAAATGACAAAACCCGGTATCGCGGGCTCCCGTTTTTTGCCGTTATAA

Upstream 100 bases:

>100_bases
GCTGGGATTTACGGTGCTGTTTGGCTTGCTATTCAGGCAAAAGCAACACAGGAAGAAGAGATGTTAGGAAAAAGTGAATG
AGTAAAAGGAGTCGCATGAA

Downstream 100 bases:

>100_bases
CAACGAAAGAGCGACTTTTGCTTTTGCAAATGGATCAATGTATGCGATAATGATATAGAAAGACTAGGGGTGCCTAATGA
AGTTAGGCTGAGAATAAGAA

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MEYRVERDLLGEKQVPKHAYYGIQSLRAKDNFPITGYPPHPELIRAFGYVKKAAALANRDVGVLRPHIAEAIIKASDEVI
EGRLNDHFIVDSIQGGAGTSFNMNANEVIANRAIEILGGEKGDYLKVSPNTHVNMAQSTNDSFPTAIHIAALNMTKGLTE
ELQKLINEMEAKAEEFDSVLKMGRTHLQDAVPIRLGQEFGSYRRVLARDLKRVSRSADHLHDINMGATAVGTGLNAKPEY
IEKVAKHLADLTELPLKTAEDLVDATQNTDAYTELSSSLKILAINLSKIANDLRLMSSGPRTGLNEINLPPRQPGSSIMP
GKVNPVMCEVINQLSFQVIGNDHTISLASEAGQLELNVMEPVLVFNLLQSLTVLQNGMRVFKDYAIAGITANVERCRELV
EHSVGIVTAINPHVGYEVATRVAKEAIETGRPVREICLERGILSEEELNEILDPKEMTKPGIAGSRFLPL

Sequences:

>Translated_470_residues
MEYRVERDLLGEKQVPKHAYYGIQSLRAKDNFPITGYPPHPELIRAFGYVKKAAALANRDVGVLRPHIAEAIIKASDEVI
EGRLNDHFIVDSIQGGAGTSFNMNANEVIANRAIEILGGEKGDYLKVSPNTHVNMAQSTNDSFPTAIHIAALNMTKGLTE
ELQKLINEMEAKAEEFDSVLKMGRTHLQDAVPIRLGQEFGSYRRVLARDLKRVSRSADHLHDINMGATAVGTGLNAKPEY
IEKVAKHLADLTELPLKTAEDLVDATQNTDAYTELSSSLKILAINLSKIANDLRLMSSGPRTGLNEINLPPRQPGSSIMP
GKVNPVMCEVINQLSFQVIGNDHTISLASEAGQLELNVMEPVLVFNLLQSLTVLQNGMRVFKDYAIAGITANVERCRELV
EHSVGIVTAINPHVGYEVATRVAKEAIETGRPVREICLERGILSEEELNEILDPKEMTKPGIAGSRFLPL
>Mature_470_residues
MEYRVERDLLGEKQVPKHAYYGIQSLRAKDNFPITGYPPHPELIRAFGYVKKAAALANRDVGVLRPHIAEAIIKASDEVI
EGRLNDHFIVDSIQGGAGTSFNMNANEVIANRAIEILGGEKGDYLKVSPNTHVNMAQSTNDSFPTAIHIAALNMTKGLTE
ELQKLINEMEAKAEEFDSVLKMGRTHLQDAVPIRLGQEFGSYRRVLARDLKRVSRSADHLHDINMGATAVGTGLNAKPEY
IEKVAKHLADLTELPLKTAEDLVDATQNTDAYTELSSSLKILAINLSKIANDLRLMSSGPRTGLNEINLPPRQPGSSIMP
GKVNPVMCEVINQLSFQVIGNDHTISLASEAGQLELNVMEPVLVFNLLQSLTVLQNGMRVFKDYAIAGITANVERCRELV
EHSVGIVTAINPHVGYEVATRVAKEAIETGRPVREICLERGILSEEELNEILDPKEMTKPGIAGSRFLPL

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=462, Percent_Identity=46.5367965367965, Blast_Score=410, Evalue=1e-114,
Organism=Escherichia coli, GI87082375, Length=467, Percent_Identity=49.2505353319058, Blast_Score=434, Evalue=1e-123,
Organism=Escherichia coli, GI1787896, Length=462, Percent_Identity=43.2900432900433, Blast_Score=377, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17553882, Length=463, Percent_Identity=45.5723542116631, Blast_Score=404, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI32565146, Length=327, Percent_Identity=42.8134556574924, Blast_Score=280, Evalue=1e-75,
Organism=Saccharomyces cerevisiae, GI6324993, Length=462, Percent_Identity=46.5367965367965, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24640177, Length=464, Percent_Identity=45.2586206896552, Blast_Score=412, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24640179, Length=464, Percent_Identity=45.2586206896552, Blast_Score=412, Evalue=1e-115,
Organism=Drosophila melanogaster, GI78710009, Length=464, Percent_Identity=43.3189655172414, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24662684, Length=465, Percent_Identity=43.2258064516129, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24583245, Length=465, Percent_Identity=40.4301075268817, Blast_Score=352, Evalue=2e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR005677
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 51576; Mature: 51576

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYRVERDLLGEKQVPKHAYYGIQSLRAKDNFPITGYPPHPELIRAFGYVKKAAALANRD
CCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
VGVLRPHIAEAIIKASDEVIEGRLNDHFIVDSIQGGAGTSFNMNANEVIANRAIEILGGE
CHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCC
KGDYLKVSPNTHVNMAQSTNDSFPTAIHIAALNMTKGLTEELQKLINEMEAKAEEFDSVL
CCCEEEECCCCCEEECCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KMGRTHLQDAVPIRLGQEFGSYRRVLARDLKRVSRSADHLHDINMGATAVGTGLNAKPEY
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCHHH
IEKVAKHLADLTELPLKTAEDLVDATQNTDAYTELSSSLKILAINLSKIANDLRLMSSGP
HHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHCCCEEEEEEHHHHHHHHHHHCCCC
RTGLNEINLPPRQPGSSIMPGKVNPVMCEVINQLSFQVIGNDHTISLASEAGQLELNVME
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCEEEECCCCEEEEECCCCCEEEHHHH
PVLVFNLLQSLTVLQNGMRVFKDYAIAGITANVERCRELVEHSVGIVTAINPHVGYEVAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCEECCHHHHHHHHHHCCCEEEEECCCCCHHHHH
RVAKEAIETGRPVREICLERGILSEEELNEILDPKEMTKPGIAGSRFLPL
HHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHCCCHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MEYRVERDLLGEKQVPKHAYYGIQSLRAKDNFPITGYPPHPELIRAFGYVKKAAALANRD
CCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
VGVLRPHIAEAIIKASDEVIEGRLNDHFIVDSIQGGAGTSFNMNANEVIANRAIEILGGE
CHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCC
KGDYLKVSPNTHVNMAQSTNDSFPTAIHIAALNMTKGLTEELQKLINEMEAKAEEFDSVL
CCCEEEECCCCCEEECCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KMGRTHLQDAVPIRLGQEFGSYRRVLARDLKRVSRSADHLHDINMGATAVGTGLNAKPEY
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCHHH
IEKVAKHLADLTELPLKTAEDLVDATQNTDAYTELSSSLKILAINLSKIANDLRLMSSGP
HHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHCCCEEEEEEHHHHHHHHHHHCCCC
RTGLNEINLPPRQPGSSIMPGKVNPVMCEVINQLSFQVIGNDHTISLASEAGQLELNVME
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCEEEECCCCEEEEECCCCCEEEHHHH
PVLVFNLLQSLTVLQNGMRVFKDYAIAGITANVERCRELVEHSVGIVTAINPHVGYEVAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCEECCHHHHHHHHHHCCCEEEEECCCCCHHHHH
RVAKEAIETGRPVREICLERGILSEEELNEILDPKEMTKPGIAGSRFLPL
HHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHCCCHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1711029; 8969508; 9384377 [H]