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The map label for this gene is thiC

Identifier: 56963498

GI number: 56963498

Start: 1861118

End: 1861747

Strand: Direct

Name: thiC

Synonym: ABC1733

Alternate gene names: 56963498

Gene position: 1861118-1861747 (Clockwise)

Preceding gene: 56963497

Following gene: 56963499

Centisome position: 43.24

GC content: 46.98

Gene sequence:

>630_bases
ATGAAGCCTTTTCGACTTTACGCGATCACAGGAGAAGAATTCCATCCTGGACGCGATGTCGTAGAAGTGATGGAAGAAGC
TATCCAAGGTGGGGTTGACATTATTCAGCTTCGTGATAAAACGAGCAGCAAAAAGGCTGTGTTGGAAAAAGCCCGTAGGC
TAAAAAAGCTTGCAGCTGATTATGGAATTCCGTTTATTGTCAATGACCATATCGATGTCGCCCTTGCCGTCGACGCAAGC
GGTGTCCATGTTGGACAAGATGATTTACCCCTTCCAGAAGTAAGAAAATTGCTGGGACCAGACAAAATCATTGGCGTTTC
TACCCACAAGTTAGAGGAAGCGCTTGAAGCTGAAAAGAACGGAGCCGATTATATTGGCGTCGGTCCGATTTTTCCAACAA
ACAGCAAAGCGGATGTAGTAGACCCAGTTACAACGGCTTATATTCGCGAAGTAAAGGAACATGTGACGATTCCATTTGTG
GCCATTGGCGGCATCAAACGCCATAATGTCCGTGAAGTGATCGAAGCGGGTGCTGAAGCCATTTGCGTTATAACCGAAAT
TGTTGCTGCCCGTGACGTAAAAGCAGCTAGTCAAGCGTTGCTTGCCGCGATGGAGGAGGCAAGTCAATGA

Upstream 100 bases:

>100_bases
CCAGCGGAGGGAAGTCGGTTTGCAGTGATTGAATGACATGCTTGCATGTGGCCGAAGCTCCTCGCTTTGGCCTTTTTATT
TTGCAAAAGGAGGGAACAAC

Downstream 100 bases:

>100_bases
GATTAGTCGTAAATGGTGAAGAACGAATTTCAGAAAGCACAACGCTGAGCGAACTAGTTTCTGAATTTGGCTTAGCGAGC
CAACTAGTGGTGGCGGAAGT

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MKPFRLYAITGEEFHPGRDVVEVMEEAIQGGVDIIQLRDKTSSKKAVLEKARRLKKLAADYGIPFIVNDHIDVALAVDAS
GVHVGQDDLPLPEVRKLLGPDKIIGVSTHKLEEALEAEKNGADYIGVGPIFPTNSKADVVDPVTTAYIREVKEHVTIPFV
AIGGIKRHNVREVIEAGAEAICVITEIVAARDVKAASQALLAAMEEASQ

Sequences:

>Translated_209_residues
MKPFRLYAITGEEFHPGRDVVEVMEEAIQGGVDIIQLRDKTSSKKAVLEKARRLKKLAADYGIPFIVNDHIDVALAVDAS
GVHVGQDDLPLPEVRKLLGPDKIIGVSTHKLEEALEAEKNGADYIGVGPIFPTNSKADVVDPVTTAYIREVKEHVTIPFV
AIGGIKRHNVREVIEAGAEAICVITEIVAARDVKAASQALLAAMEEASQ
>Mature_209_residues
MKPFRLYAITGEEFHPGRDVVEVMEEAIQGGVDIIQLRDKTSSKKAVLEKARRLKKLAADYGIPFIVNDHIDVALAVDAS
GVHVGQDDLPLPEVRKLLGPDKIIGVSTHKLEEALEAEKNGADYIGVGPIFPTNSKADVVDPVTTAYIREVKEHVTIPFV
AIGGIKRHNVREVIEAGAEAICVITEIVAARDVKAASQALLAAMEEASQ

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=203, Percent_Identity=31.0344827586207, Blast_Score=79, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6325042, Length=216, Percent_Identity=36.5740740740741, Blast_Score=132, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_BACSK (Q5WH87)

Other databases:

- EMBL:   AP006627
- RefSeq:   YP_175229.1
- HSSP:   P39594
- ProteinModelPortal:   Q5WH87
- STRING:   Q5WH87
- EnsemblBacteria:   EBBACT00000047601
- GeneID:   3203412
- GenomeReviews:   AP006627_GR
- KEGG:   bcl:ABC1733
- NMPDR:   fig|66692.3.peg.1681
- eggNOG:   COG0352
- GeneTree:   EBGT00050000001882
- HOGENOM:   HBG754477
- OMA:   GARCCAL
- ProtClustDB:   CLSK248057
- BioCyc:   BCLA66692:ABC1733-MONOMER
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 22579; Mature: 22579

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPFRLYAITGEEFHPGRDVVEVMEEAIQGGVDIIQLRDKTSSKKAVLEKARRLKKLAAD
CCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
YGIPFIVNDHIDVALAVDASGVHVGQDDLPLPEVRKLLGPDKIIGVSTHKLEEALEAEKN
CCCCEEECCCCEEEEEECCCCCEECCCCCCHHHHHHHHCCCCEECCHHHHHHHHHHHHCC
GADYIGVGPIFPTNSKADVVDPVTTAYIREVKEHVTIPFVAIGGIKRHNVREVIEAGAEA
CCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHCCCHH
ICVITEIVAARDVKAASQALLAAMEEASQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKPFRLYAITGEEFHPGRDVVEVMEEAIQGGVDIIQLRDKTSSKKAVLEKARRLKKLAAD
CCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
YGIPFIVNDHIDVALAVDASGVHVGQDDLPLPEVRKLLGPDKIIGVSTHKLEEALEAEKN
CCCCEEECCCCEEEEEECCCCCEECCCCCCHHHHHHHHCCCCEECCHHHHHHHHHHHHCC
GADYIGVGPIFPTNSKADVVDPVTTAYIREVKEHVTIPFVAIGGIKRHNVREVIEAGAEA
CCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHCCCHH
ICVITEIVAARDVKAASQALLAAMEEASQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA