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The map label for this gene is rsmE [H]

Identifier: 56963427

GI number: 56963427

Start: 1792480

End: 1793226

Strand: Direct

Name: rsmE [H]

Synonym: ABC1662

Alternate gene names: 56963427

Gene position: 1792480-1793226 (Clockwise)

Preceding gene: 56963426

Following gene: 56963428

Centisome position: 41.65

GC content: 49.13

Gene sequence:

>747_bases
ATGCAGCGTTACTTTATTAAAAATGAACAGTTGACAAATGAAGTTGTCGAAATAACTGGCGACGACGCCGCGCACATTAC
AAAAGTGATGCGTATGCAAGCAGGCGATGAAGTCATTTGCGTCAATGAAGACGGCAAAGCCGTGCTTGTTCAGCTTTCGC
AATTCGACCCATCTAGCGTCAAAGGCACCGTTCTCAACGAAATGGACAACGAGACAGAATTGCCAATCCGTGTGACGGTC
GCGCAAGGCCTGCCGAAAGGCGATAAGCTTGAAACCATCGTCCAAAAAGGAACAGAACTCGGCGCGTACGCATTCGCGCC
TTTTTCTGCGAGTCGATCTATAGTAAAATGGGATGAAAAGAAGGGTGCAAAAAAAACGGAGCGTTTGCAAAAGATTGCTA
AGGAAGCCGCAGAACAAGCACATCGCCTTTTCGTGCCAAACGTGGCCAAGCCTGTGCCGCTAAAAGTGGTTGCCGAGGCC
CTTACTCATTACGATGTCTGCATTGTTTGTGATGAAGAAGAAGCGAAGCAAGGTGAAAAGGCAAACTTAAAGCGCGCCTT
TGCTATGCTCAAACCAGGCCTGTCTCTCTTGGTCATTGTTGGTCCTGAAGGCGGGCTGACTCGTGAGGAAGTAGCGCTTT
TGTCGGCGAAGGGCGCGGTCTCTTGCAGCATAGGCCCTCGCATTGTCCGTACTGAAACGGCTGCTATGTACGTGTTAGCT
GCTCTTTCGTACCAATTTGAATTATGA

Upstream 100 bases:

>100_bases
TAAAGGCAGTAGGTTTTCTGATTCAGGAAGTGAACGAATTGGACGACTGGGTAGCGATTGTTGCGCAAAAACCCAGCCAA
GCGTAGGTAGGTGCAAAGGC

Downstream 100 bases:

>100_bases
GGTGAATCCAGTTGTCAACTGTTGCGTTTCATACATTAGGCTGTAAAGTAAACCATTACGAGACGGAAGCGATTTGGCAG
CTGTTTAAACAAGAAGGCTA

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MQRYFIKNEQLTNEVVEITGDDAAHITKVMRMQAGDEVICVNEDGKAVLVQLSQFDPSSVKGTVLNEMDNETELPIRVTV
AQGLPKGDKLETIVQKGTELGAYAFAPFSASRSIVKWDEKKGAKKTERLQKIAKEAAEQAHRLFVPNVAKPVPLKVVAEA
LTHYDVCIVCDEEEAKQGEKANLKRAFAMLKPGLSLLVIVGPEGGLTREEVALLSAKGAVSCSIGPRIVRTETAAMYVLA
ALSYQFEL

Sequences:

>Translated_248_residues
MQRYFIKNEQLTNEVVEITGDDAAHITKVMRMQAGDEVICVNEDGKAVLVQLSQFDPSSVKGTVLNEMDNETELPIRVTV
AQGLPKGDKLETIVQKGTELGAYAFAPFSASRSIVKWDEKKGAKKTERLQKIAKEAAEQAHRLFVPNVAKPVPLKVVAEA
LTHYDVCIVCDEEEAKQGEKANLKRAFAMLKPGLSLLVIVGPEGGLTREEVALLSAKGAVSCSIGPRIVRTETAAMYVLA
ALSYQFEL
>Mature_248_residues
MQRYFIKNEQLTNEVVEITGDDAAHITKVMRMQAGDEVICVNEDGKAVLVQLSQFDPSSVKGTVLNEMDNETELPIRVTV
AQGLPKGDKLETIVQKGTELGAYAFAPFSASRSIVKWDEKKGAKKTERLQKIAKEAAEQAHRLFVPNVAKPVPLKVVAEA
LTHYDVCIVCDEEEAKQGEKANLKRAFAMLKPGLSLLVIVGPEGGLTREEVALLSAKGAVSCSIGPRIVRTETAAMYVLA
ALSYQFEL

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=231, Percent_Identity=30.3030303030303, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 27058; Mature: 27058

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRYFIKNEQLTNEVVEITGDDAAHITKVMRMQAGDEVICVNEDGKAVLVQLSQFDPSSV
CCCEEECCHHHHHHHEEECCCCHHHHHHHHHHCCCCCEEEECCCCCEEEEEEECCCCCCC
KGTVLNEMDNETELPIRVTVAQGLPKGDKLETIVQKGTELGAYAFAPFSASRSIVKWDEK
CCHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHCCCCCCCEEECCCCCCCCCEEEHHH
KGAKKTERLQKIAKEAAEQAHRLFVPNVAKPVPLKVVAEALTHYDVCIVCDEEEAKQGEK
CCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHCCEEEEECCHHHHCCCH
ANLKRAFAMLKPGLSLLVIVGPEGGLTREEVALLSAKGAVSCSIGPRIVRTETAAMYVLA
HHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHEECCCCEEECCCCEEEEHHHHHHHHHH
ALSYQFEL
HHHHHCCC
>Mature Secondary Structure
MQRYFIKNEQLTNEVVEITGDDAAHITKVMRMQAGDEVICVNEDGKAVLVQLSQFDPSSV
CCCEEECCHHHHHHHEEECCCCHHHHHHHHHHCCCCCEEEECCCCCEEEEEEECCCCCCC
KGTVLNEMDNETELPIRVTVAQGLPKGDKLETIVQKGTELGAYAFAPFSASRSIVKWDEK
CCHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHCCCCCCCEEECCCCCCCCCEEEHHH
KGAKKTERLQKIAKEAAEQAHRLFVPNVAKPVPLKVVAEALTHYDVCIVCDEEEAKQGEK
CCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHCCEEEEECCHHHHCCCH
ANLKRAFAMLKPGLSLLVIVGPEGGLTREEVALLSAKGAVSCSIGPRIVRTETAAMYVLA
HHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHEECCCCEEECCCCEEEEHHHHHHHHHH
ALSYQFEL
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9023197; 9384377 [H]