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The map label for this gene is nfrA1 [H]

Identifier: 56963256

GI number: 56963256

Start: 1611715

End: 1612443

Strand: Direct

Name: nfrA1 [H]

Synonym: ABC1491

Alternate gene names: 56963256

Gene position: 1611715-1612443 (Clockwise)

Preceding gene: 56963255

Following gene: 56963257

Centisome position: 37.45

GC content: 49.25

Gene sequence:

>729_bases
GTGCTAAAGCTTATGACCAGTCATCGGTCGATTCGCGCTTTTACAAATAAGCGCGTCACAAAGGAGCAAATTGAAACGAT
TATCAGCTCGGCGCAGTGGGCGTCGACGTCCCATCATGTGCAAGCGTACTCCGTTATAACTGTGGAGGATGCTGCTCGTA
AAGAAACACTCGCCGATTTAACAGGCGGCCAACGTTGGGTAAAGGAGTGTAGTGTGTTTTTTGTCATTTGCGCAGATTTT
TCACGGCATGCAAGTGCCAGCAAACAGCATCACGTTTCATTTGACGTCGGTGGGGCGGAGCAACTTCTCGTTGGCGCTGT
GGATGCCGCTTTATTTGCCCAAAATGTTATGCTTGCCGCTGAATCGCTTGGCCTTGGCGGTGTCATGATTGGCGGAATCC
GCAATCAACCAAAGGAAGTAAGCGACTCGCTTCATTTGCCTACATATGTATTCCCAGTAATGGGGATGGCGATAGGCGAG
CCTGACAGTGAACCTGGGCAAAAGCCGCGCCTGCCTTTAAAAGCTGTGCTCCACCAAGAAACATATCAAAGCGATCAGGA
AGATGCCCTTTCTGCCTATGATGAAACAATGAGCGCTTATTATGCGAAGCGCACGAATGGCAAACGAACCAATGGCTGGA
CAGCATCGATGGCGGCTTACACAAAAGAACGGAAGCGGCCTCATTTGGATGCTTTCTTGAAAGAAAAGGGCTTTTTGCAA
CAGGAATAA

Upstream 100 bases:

>100_bases
GATCCTGCCGCTAATAGTTCCTCTTTTTCGGCATTTTTAGTACACTACCATTATCAGCAAAAAAATGAAACAGGTGGTGG
TGGAGATGGAAAGCAATGAT

Downstream 100 bases:

>100_bases
CAGACAGTGAGTGGGGGGATGGCGATGGACATACAGCAAGCAAAAATGATGGAACAGCTGCCAAGTCAATTTTTTGCGAA
ATTAGTAAACAAAGCACAAC

Product: nitro/flavin reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MLKLMTSHRSIRAFTNKRVTKEQIETIISSAQWASTSHHVQAYSVITVEDAARKETLADLTGGQRWVKECSVFFVICADF
SRHASASKQHHVSFDVGGAEQLLVGAVDAALFAQNVMLAAESLGLGGVMIGGIRNQPKEVSDSLHLPTYVFPVMGMAIGE
PDSEPGQKPRLPLKAVLHQETYQSDQEDALSAYDETMSAYYAKRTNGKRTNGWTASMAAYTKERKRPHLDAFLKEKGFLQ
QE

Sequences:

>Translated_242_residues
MLKLMTSHRSIRAFTNKRVTKEQIETIISSAQWASTSHHVQAYSVITVEDAARKETLADLTGGQRWVKECSVFFVICADF
SRHASASKQHHVSFDVGGAEQLLVGAVDAALFAQNVMLAAESLGLGGVMIGGIRNQPKEVSDSLHLPTYVFPVMGMAIGE
PDSEPGQKPRLPLKAVLHQETYQSDQEDALSAYDETMSAYYAKRTNGKRTNGWTASMAAYTKERKRPHLDAFLKEKGFLQ
QE
>Mature_242_residues
MLKLMTSHRSIRAFTNKRVTKEQIETIISSAQWASTSHHVQAYSVITVEDAARKETLADLTGGQRWVKECSVFFVICADF
SRHASASKQHHVSFDVGGAEQLLVGAVDAALFAQNVMLAAESLGLGGVMIGGIRNQPKEVSDSLHLPTYVFPVMGMAIGE
PDSEPGQKPRLPLKAVLHQETYQSDQEDALSAYDETMSAYYAKRTNGKRTNGWTASMAAYTKERKRPHLDAFLKEKGFLQ
QE

Specific function: Reduction Of Nitroaromatic Compounds Using NADH. Reduces Nitrofurazone By A Ping-Pong Bi-Bi Mechanism Possibly To Generate A Two-Electron Transfer Product. Major Component Of The Oxygen- Insensitive Nitroreductase Activity In E.Coli. [C]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the flavin oxidoreductase frp family [H]

Homologues:

Organism=Escherichia coli, GI1787075, Length=237, Percent_Identity=40.9282700421941, Blast_Score=187, Evalue=4e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016446
- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 26748; Mature: 26748

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKLMTSHRSIRAFTNKRVTKEQIETIISSAQWASTSHHVQAYSVITVEDAARKETLADL
CCEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEECHHHHHHHHHHH
TGGQRWVKECSVFFVICADFSRHASASKQHHVSFDVGGAEQLLVGAVDAALFAQNVMLAA
CCHHHHHHHHHEEEEEECCHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
ESLGLGGVMIGGIRNQPKEVSDSLHLPTYVFPVMGMAIGEPDSEPGQKPRLPLKAVLHQE
HHCCCCCEEECCCCCCCHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
TYQSDQEDALSAYDETMSAYYAKRTNGKRTNGWTASMAAYTKERKRPHLDAFLKEKGFLQ
HHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCC
QE
CC
>Mature Secondary Structure
MLKLMTSHRSIRAFTNKRVTKEQIETIISSAQWASTSHHVQAYSVITVEDAARKETLADL
CCEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEECHHHHHHHHHHH
TGGQRWVKECSVFFVICADFSRHASASKQHHVSFDVGGAEQLLVGAVDAALFAQNVMLAA
CCHHHHHHHHHEEEEEECCHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
ESLGLGGVMIGGIRNQPKEVSDSLHLPTYVFPVMGMAIGEPDSEPGQKPRLPLKAVLHQE
HHCCCCCEEECCCCCCCHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
TYQSDQEDALSAYDETMSAYYAKRTNGKRTNGWTASMAAYTKERKRPHLDAFLKEKGFLQ
HHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCC
QE
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA