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The map label for this gene is hacA [H]

Identifier: 55981880

GI number: 55981880

Start: 1793647

End: 1794909

Strand: Reverse

Name: hacA [H]

Synonym: TTHA1911

Alternate gene names: 55981880

Gene position: 1794909-1793647 (Counterclockwise)

Preceding gene: 55981883

Following gene: 55981879

Centisome position: 97.04

GC content: 69.75

Gene sequence:

>1263_bases
GTGTCCGTGGGACAGACGCTAGCGGAAAAGATCCTCTCCCACAAGGTGGGAAGGCCCGTGCGGGCGGGGGAGCTCGTGGT
GGTGGAGGTGGACCAGGTGATGGTGGTGGACTCCATCGCCGGGAGCTTCTTCAAGCGCCTGGAGTACCTGGAGGCCACCC
CCCGCTACCCGGAAAGGGTCTCCATCGTGATAGACCACGTGGCCCCGGCGGCGAACCTGGAGGTGGCCAAGGCGCAGAAG
GAGATCCGGGAGTGGGGCAAGCGGCACGGCATCCGGGTCTTTGACGTGGGAAGGGGGGTGTGCCACCAGGTCCTCATAGA
GGAGGGCCTGGCCCAGCCGGGATGGGTGGTGGTGGGCTCGGACAGCCACTCCACCACCTACGGGGCGGTGGGGGCCTTCG
GCACGGGGATGGGGGCCACGGACATCGCCTTGGCGGCGGCGAGCGGGCGCACCTGGCTTAGGGTGCCCGAGAGCGTCAAG
GTGGTCTTCCGGGGAAGGCTCCCCAAGGGGGTCACGGCCAAGGACGCCGCCCTGGAGATGGTCCGCCTCCTCACCGCCGA
AGGGGCCACCTACATGGCGGTGGAGATCCACCTCTTAGACGGGGCGGAGGCCCTCACGCGGGGCGAGCGCATGACCCTCG
CCAACCTCACCGTGGAGGCGGGGGCCAAGGCGGGGCTCGTGGTGCCGAGTGGGGAGATCCTGGAGATGTACCGGGTGCCC
GACTGGCTCTATCCCGACCCCGACGCCCGCTACGCCAAGGAGGTGGAGATAGACCTTTCCGCCCTCACCCCCAGGGTCTC
CGTGCCCTTCTACGTGGACAACGTCCACGAGGTGGCCCAGGTCAAGGGCAAGCGGGTGGACCAGGTCTTCATCGGCACCT
GCACCAACGGGCGCATTGAGGACTTGAGGGCGGCGGCGGAGGTGCTAAAGGGGAGGAAGGTGGCCCCTTGGGTGCGGCTT
TTGGTGGTCCCGGCGAGCTCCCAGGTCCTGGAGGAGGCGGCCAGGGACGGCACCCTCCTCACCCTCCTCGAGGCCGGGGC
CACCATTGGCACCCCGGGGTGCGGCCCCTGCATGGGGCGGCACATGGGGGTCTTGGCCCCGGGGGAGGTGTGCGTCTCCA
CCTCCAACCGCAACTTCCGGGGGCGCATGGGGGCCCCGGACGCCGAGATCTACCTGGCGAGCCCCCGCGTGGCCGCGGCG
AGCGCCGTGGCGGGCTACCTCACCACCCCAGAGGAGCTGGAAGAGGAGGAGGTCCATGCCTAG

Upstream 100 bases:

>100_bases
GGCTTGGGGCACCGCGGGAAACTCGGCGATCCAGACCCCTGGGGTCTCCGGGTCCGGGTAGAGGAGGGCGGTGTACTTGA
GCATACCCTCAGTATAGGAG

Downstream 100 bases:

>100_bases
GGTCTGGAAGTTCGGCGACCAGATCAACACCGACGACATCCTTCCCGGAAAGTACGCCCCCTTCATGGTGGGGGAGGACC
GGTTTCACCTCTACGCCTTC

Product: 3-isopropylmalate dehydratase large subunit

Products: NA

Alternate protein names: HACN; Homoaconitate hydratase [H]

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MSVGQTLAEKILSHKVGRPVRAGELVVVEVDQVMVVDSIAGSFFKRLEYLEATPRYPERVSIVIDHVAPAANLEVAKAQK
EIREWGKRHGIRVFDVGRGVCHQVLIEEGLAQPGWVVVGSDSHSTTYGAVGAFGTGMGATDIALAAASGRTWLRVPESVK
VVFRGRLPKGVTAKDAALEMVRLLTAEGATYMAVEIHLLDGAEALTRGERMTLANLTVEAGAKAGLVVPSGEILEMYRVP
DWLYPDPDARYAKEVEIDLSALTPRVSVPFYVDNVHEVAQVKGKRVDQVFIGTCTNGRIEDLRAAAEVLKGRKVAPWVRL
LVVPASSQVLEEAARDGTLLTLLEAGATIGTPGCGPCMGRHMGVLAPGEVCVSTSNRNFRGRMGAPDAEIYLASPRVAAA
SAVAGYLTTPEELEEEEVHA

Sequences:

>Translated_420_residues
MSVGQTLAEKILSHKVGRPVRAGELVVVEVDQVMVVDSIAGSFFKRLEYLEATPRYPERVSIVIDHVAPAANLEVAKAQK
EIREWGKRHGIRVFDVGRGVCHQVLIEEGLAQPGWVVVGSDSHSTTYGAVGAFGTGMGATDIALAAASGRTWLRVPESVK
VVFRGRLPKGVTAKDAALEMVRLLTAEGATYMAVEIHLLDGAEALTRGERMTLANLTVEAGAKAGLVVPSGEILEMYRVP
DWLYPDPDARYAKEVEIDLSALTPRVSVPFYVDNVHEVAQVKGKRVDQVFIGTCTNGRIEDLRAAAEVLKGRKVAPWVRL
LVVPASSQVLEEAARDGTLLTLLEAGATIGTPGCGPCMGRHMGVLAPGEVCVSTSNRNFRGRMGAPDAEIYLASPRVAAA
SAVAGYLTTPEELEEEEVHA
>Mature_419_residues
SVGQTLAEKILSHKVGRPVRAGELVVVEVDQVMVVDSIAGSFFKRLEYLEATPRYPERVSIVIDHVAPAANLEVAKAQKE
IREWGKRHGIRVFDVGRGVCHQVLIEEGLAQPGWVVVGSDSHSTTYGAVGAFGTGMGATDIALAAASGRTWLRVPESVKV
VFRGRLPKGVTAKDAALEMVRLLTAEGATYMAVEIHLLDGAEALTRGERMTLANLTVEAGAKAGLVVPSGEILEMYRVPD
WLYPDPDARYAKEVEIDLSALTPRVSVPFYVDNVHEVAQVKGKRVDQVFIGTCTNGRIEDLRAAAEVLKGRKVAPWVRLL
VVPASSQVLEEAARDGTLLTLLEAGATIGTPGCGPCMGRHMGVLAPGEVCVSTSNRNFRGRMGAPDAEIYLASPRVAAAS
AVAGYLTTPEELEEEEVHA

Specific function: Catalyzes the reversible hydration of cis-homoaconitate ((Z)-but-1-ene-1,2,4-tricarboxylate) to homoisocitrate ((1R,2S)-1- hydroxybutane-1,2,4-tricarboxylate). Can catalyze neither the dehydration of (R)-homocitrate ((2R)-2-hydroxybutane-1,2,4- tricarboxy

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=360, Percent_Identity=31.1111111111111, Blast_Score=134, Evalue=1e-31,
Organism=Escherichia coli, GI1786259, Length=462, Percent_Identity=31.1688311688312, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI87081781, Length=362, Percent_Identity=29.2817679558011, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI2367097, Length=361, Percent_Identity=31.8559556786704, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1787531, Length=407, Percent_Identity=26.2899262899263, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI25149337, Length=447, Percent_Identity=27.9642058165548, Blast_Score=131, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI32564738, Length=351, Percent_Identity=29.3447293447293, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI25149342, Length=284, Percent_Identity=29.2253521126761, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320440, Length=452, Percent_Identity=35.1769911504425, Blast_Score=233, Evalue=6e-62,
Organism=Saccharomyces cerevisiae, GI6321429, Length=478, Percent_Identity=28.0334728033473, Blast_Score=167, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6322261, Length=457, Percent_Identity=27.5711159737418, Blast_Score=147, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6323335, Length=448, Percent_Identity=28.125, Blast_Score=145, Evalue=1e-35,
Organism=Drosophila melanogaster, GI28571643, Length=448, Percent_Identity=27.6785714285714, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI281365315, Length=447, Percent_Identity=25.503355704698, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI17864292, Length=447, Percent_Identity=25.503355704698, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI161076999, Length=349, Percent_Identity=26.0744985673352, Blast_Score=111, Evalue=9e-25,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.36 [H]

Molecular weight: Translated: 44978; Mature: 44847

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVGQTLAEKILSHKVGRPVRAGELVVVEVDQVMVVDSIAGSFFKRLEYLEATPRYPERV
CCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCEEEHHHHHHHHHHHHHHHHCCCCCCCEE
SIVIDHVAPAANLEVAKAQKEIREWGKRHGIRVFDVGRGVCHQVLIEEGLAQPGWVVVGS
EEEEECCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHCCCCCCEEEEEC
DSHSTTYGAVGAFGTGMGATDIALAAASGRTWLRVPESVKVVFRGRLPKGVTAKDAALEM
CCCCCCCCCHHCCCCCCCCHHHEEEECCCCEEEECCHHEEEEEECCCCCCCCHHHHHHHH
VRLLTAEGATYMAVEIHLLDGAEALTRGERMTLANLTVEAGAKAGLVVPSGEILEMYRVP
HHHHHCCCCEEEEEEEEEECCHHHHHCCCEEEEEEEEEECCCCCCEEECCHHHHHEECCC
DWLYPDPDARYAKEVEIDLSALTPRVSVPFYVDNVHEVAQVKGKRVDQVFIGTCTNGRIE
CCCCCCCCCCCHHEEEEEHHHCCCCCCCCEEECCHHHHHHHCCCCCCEEEEEECCCCCHH
DLRAAAEVLKGRKVAPWVRLLVVPASSQVLEEAARDGTLLTLLEAGATIGTPGCGPCMGR
HHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHC
HMGVLAPGEVCVSTSNRNFRGRMGAPDAEIYLASPRVAAASAVAGYLTTPEELEEEEVHA
CCCCCCCCCEEEECCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCCCHHHHHHHHCCC
>Mature Secondary Structure 
SVGQTLAEKILSHKVGRPVRAGELVVVEVDQVMVVDSIAGSFFKRLEYLEATPRYPERV
CHHHHHHHHHHHHHCCCCCCCCCEEEEEECCEEEHHHHHHHHHHHHHHHHCCCCCCCEE
SIVIDHVAPAANLEVAKAQKEIREWGKRHGIRVFDVGRGVCHQVLIEEGLAQPGWVVVGS
EEEEECCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHCCCCCCEEEEEC
DSHSTTYGAVGAFGTGMGATDIALAAASGRTWLRVPESVKVVFRGRLPKGVTAKDAALEM
CCCCCCCCCHHCCCCCCCCHHHEEEECCCCEEEECCHHEEEEEECCCCCCCCHHHHHHHH
VRLLTAEGATYMAVEIHLLDGAEALTRGERMTLANLTVEAGAKAGLVVPSGEILEMYRVP
HHHHHCCCCEEEEEEEEEECCHHHHHCCCEEEEEEEEEECCCCCCEEECCHHHHHEECCC
DWLYPDPDARYAKEVEIDLSALTPRVSVPFYVDNVHEVAQVKGKRVDQVFIGTCTNGRIE
CCCCCCCCCCCHHEEEEEHHHCCCCCCCCEEECCHHHHHHHCCCCCCEEEEEECCCCCHH
DLRAAAEVLKGRKVAPWVRLLVVPASSQVLEEAARDGTLLTLLEAGATIGTPGCGPCMGR
HHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHC
HMGVLAPGEVCVSTSNRNFRGRMGAPDAEIYLASPRVAAASAVAGYLTTPEELEEEEVHA
CCCCCCCCCEEEECCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10074061; 9868782 [H]