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The map label for this gene is lys20

Identifier: 55981883

GI number: 55981883

Start: 1795349

End: 1796479

Strand: Reverse

Name: lys20

Synonym: TTHA1914

Alternate gene names: 55981883

Gene position: 1796479-1795349 (Counterclockwise)

Preceding gene: 55981884

Following gene: 55981880

Centisome position: 97.12

GC content: 66.49

Gene sequence:

>1131_bases
ATGCGGGAGTGGAAGATTATTGACTCCACCTTACGGGAAGGGGAACAGTTTGAAAAGGCGAACTTCTCCACCCAAGACAA
GGTGGAGATCGCCAAGGCCCTGGACGAGTTCGGCATTGAGTACATTGAGGTCACCACCCCGGTGGCCTCCCCCCAGTCCC
GCAAGGACGCCGAGGTCCTCGCCTCCTTGGGCCTCAAGGCCAAGGTGGTGACCCACATCCAGTGCCGCCTGGACGCGGCC
AAGGTGGCGGTGGAGACGGGAGTCCAGGGGATTGACCTCCTCTTCGGCACGAGCAAGTACCTCCGGGCCGCCCATGGGCG
GGACATCCCCAGGATCATTGAGGAGGCCAAAGAGGTGATCGCCTACATCCGCGAGGCGGCCCCCCACGTGGAGGTGCGCT
TCTCCGCCGAGGACACCTTCCGCTCCGAGGAGCAGGACCTCCTCGCCGTCTACGAGGCCGTGGCCCCCTACGTGGACCGG
GTGGGCCTGGCGGACACCGTGGGCGTGGCCACGCCCCGGCAGGTCTACGCCCTGGTGCGGGAGGTGCGGCGGGTGGTGGG
GCCCAGGGTGGACATTGAGTTCCACGGCCACAACGACACCGGGTGCGCCATCGCCAACGCGTATGAGGCCATTGAGGCCG
GGGCCACCCACGTGGACACCACCATCCTGGGGATCGGGGAGAGGAACGGGATCACGCCTTTGGGGGGGTTCCTCGCCCGC
ATGTACACCCTCCAGCCTGAGTACGTGCGCAGGAAGTACAAGCTGGAGATGCTCCCCGAGCTGGACCGGATGGTGGCCCG
GATGGTGGGGGTGGAGATTCCCTTCAACAACTACATCACCGGGGAGACGGCCTTCAGCCACAAGGCGGGGATGCACCTCA
AGGCCATCTACATCAACCCCGAGGCCTACGAGCCCTACCCCCCGGAGGTCTTCGGGGTGAAGCGGAAGCTCATCATCGCC
TCGAGGCTCACCGGGCGGCACGCCATCAAGGCGCGGGCGGAGGAGCTCGGCCTCCACTACGGGGAGGAGGAGCTCCACCG
GGTCACCCAGCACATCAAGGCCCTGGCCGACCGGGGCCAGCTCACCCTGGAGGAGCTGGACCGGATCCTCCGGGAGTGGA
TCACGGCGTGA

Upstream 100 bases:

>100_bases
CCGCGCGAAAAAACTCGGCCGCCGGGGGGTAGGGTAGCCCTCCAAGGGCTTCTTATCCCCCGGCCCGGGTTCGGGCCGGT
TTTCTTTTGGGGAGGGGTGC

Downstream 100 bases:

>100_bases
GGCTAGAGGTTATGGAACTCCTCCTCGGTGAGCCCGGCATCGCGCAGGATGCGCTTGAAGGTGCCCTTGGGGAGTTCCCC
GCTGTGGAAGGGCACCACCA

Product: homocitrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MREWKIIDSTLREGEQFEKANFSTQDKVEIAKALDEFGIEYIEVTTPVASPQSRKDAEVLASLGLKAKVVTHIQCRLDAA
KVAVETGVQGIDLLFGTSKYLRAAHGRDIPRIIEEAKEVIAYIREAAPHVEVRFSAEDTFRSEEQDLLAVYEAVAPYVDR
VGLADTVGVATPRQVYALVREVRRVVGPRVDIEFHGHNDTGCAIANAYEAIEAGATHVDTTILGIGERNGITPLGGFLAR
MYTLQPEYVRRKYKLEMLPELDRMVARMVGVEIPFNNYITGETAFSHKAGMHLKAIYINPEAYEPYPPEVFGVKRKLIIA
SRLTGRHAIKARAEELGLHYGEEELHRVTQHIKALADRGQLTLEELDRILREWITA

Sequences:

>Translated_376_residues
MREWKIIDSTLREGEQFEKANFSTQDKVEIAKALDEFGIEYIEVTTPVASPQSRKDAEVLASLGLKAKVVTHIQCRLDAA
KVAVETGVQGIDLLFGTSKYLRAAHGRDIPRIIEEAKEVIAYIREAAPHVEVRFSAEDTFRSEEQDLLAVYEAVAPYVDR
VGLADTVGVATPRQVYALVREVRRVVGPRVDIEFHGHNDTGCAIANAYEAIEAGATHVDTTILGIGERNGITPLGGFLAR
MYTLQPEYVRRKYKLEMLPELDRMVARMVGVEIPFNNYITGETAFSHKAGMHLKAIYINPEAYEPYPPEVFGVKRKLIIA
SRLTGRHAIKARAEELGLHYGEEELHRVTQHIKALADRGQLTLEELDRILREWITA
>Mature_376_residues
MREWKIIDSTLREGEQFEKANFSTQDKVEIAKALDEFGIEYIEVTTPVASPQSRKDAEVLASLGLKAKVVTHIQCRLDAA
KVAVETGVQGIDLLFGTSKYLRAAHGRDIPRIIEEAKEVIAYIREAAPHVEVRFSAEDTFRSEEQDLLAVYEAVAPYVDR
VGLADTVGVATPRQVYALVREVRRVVGPRVDIEFHGHNDTGCAIANAYEAIEAGATHVDTTILGIGERNGITPLGGFLAR
MYTLQPEYVRRKYKLEMLPELDRMVARMVGVEIPFNNYITGETAFSHKAGMHLKAIYINPEAYEPYPPEVFGVKRKLIIA
SRLTGRHAIKARAEELGLHYGEEELHRVTQHIKALADRGQLTLEELDRILREWITA

Specific function: Catalyzes The Condensation Of The Acetyl Group Of Acetyl-CoA With 3-Methyl-2-Oxobutanoate (2-Oxoisovalerate) To Form 3-Carboxy-3-Hydroxy-4-Methylpentanoate (2-Isopropylmalate). [C]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family

Homologues:

Organism=Escherichia coli, GI1786261, Length=368, Percent_Identity=30.4347826086957, Blast_Score=136, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6320019, Length=375, Percent_Identity=54.1333333333333, Blast_Score=419, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6320071, Length=375, Percent_Identity=53.8666666666667, Blast_Score=419, Evalue=1e-118,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): HOSC_THET2 (O87198)

Other databases:

- EMBL:   AB018379
- EMBL:   AE017221
- PIR:   T51170
- RefSeq:   YP_005519.1
- PDB:   2ZTJ
- PDB:   2ZTK
- PDB:   2ZYF
- PDB:   3A9I
- PDBsum:   2ZTJ
- PDBsum:   2ZTK
- PDBsum:   2ZYF
- PDBsum:   3A9I
- ProteinModelPortal:   O87198
- SMR:   O87198
- STRING:   O87198
- GeneID:   2775799
- GenomeReviews:   AE017221_GR
- eggNOG:   COG0119
- HOGENOM:   HBG701974
- OMA:   ERTGNTA
- PhylomeDB:   O87198
- ProtClustDB:   CLSK881007
- BioCyc:   MetaCyc:MONOMER-6722
- BioCyc:   TTHE262724:TT_C1550-MONOMER
- GO:   GO:0005737
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR011872
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR10277:SF8
- TIGRFAMs:   TIGR02146

Pfam domain/function: PF00682 HMGL-like

EC number: =2.3.3.14

Molecular weight: Translated: 42160; Mature: 42160

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREWKIIDSTLREGEQFEKANFSTQDKVEIAKALDEFGIEYIEVTTPVASPQSRKDAEVL
CCCCHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHH
ASLGLKAKVVTHIQCRLDAAKVAVETGVQGIDLLFGTSKYLRAAHGRDIPRIIEEAKEVI
HHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHH
AYIREAAPHVEVRFSAEDTFRSEEQDLLAVYEAVAPYVDRVGLADTVGVATPRQVYALVR
HHHHHCCCCEEEEECCHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHH
EVRRVVGPRVDIEFHGHNDTGCAIANAYEAIEAGATHVDTTILGIGERNGITPLGGFLAR
HHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHH
MYTLQPEYVRRKYKLEMLPELDRMVARMVGVEIPFNNYITGETAFSHKAGMHLKAIYINP
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEECC
EAYEPYPPEVFGVKRKLIIASRLTGRHAIKARAEELGLHYGEEELHRVTQHIKALADRGQ
CCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCC
LTLEELDRILREWITA
EEHHHHHHHHHHHHCC
>Mature Secondary Structure
MREWKIIDSTLREGEQFEKANFSTQDKVEIAKALDEFGIEYIEVTTPVASPQSRKDAEVL
CCCCHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHH
ASLGLKAKVVTHIQCRLDAAKVAVETGVQGIDLLFGTSKYLRAAHGRDIPRIIEEAKEVI
HHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHH
AYIREAAPHVEVRFSAEDTFRSEEQDLLAVYEAVAPYVDRVGLADTVGVATPRQVYALVR
HHHHHCCCCEEEEECCHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHH
EVRRVVGPRVDIEFHGHNDTGCAIANAYEAIEAGATHVDTTILGIGERNGITPLGGFLAR
HHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHH
MYTLQPEYVRRKYKLEMLPELDRMVARMVGVEIPFNNYITGETAFSHKAGMHLKAIYINP
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEECC
EAYEPYPPEVFGVKRKLIIASRLTGRHAIKARAEELGLHYGEEELHRVTQHIKALADRGQ
CCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCC
LTLEELDRILREWITA
EEHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9868782