Click here to switch to the map view.

The map label for this gene is metK

Identifier: 55981611

GI number: 55981611

Start: 1556998

End: 1558185

Strand: Reverse

Name: metK

Synonym: TTHA1642

Alternate gene names: 55981611

Gene position: 1558185-1556998 (Counterclockwise)

Preceding gene: 55981614

Following gene: 55981604

Centisome position: 84.24

GC content: 68.86

Gene sequence:

>1188_bases
GTGCGCGCGTTGAGGCTGGTCACGTCTGAATCGGTTACCGAGGGGCACCCGGATAAGCTGGCCGACCGGATCTCCGACGC
CATCCTGGACGCCCTCATCGCCCAGGACAAGAAGGCCCGGGTGGCGGCGGAGACCCTGGTGACCACCGGGCTCGTCTTCG
TGGCCGGGGAGATCACCACCGAGGGGTACGTGGACATCCCCAACCTGGTGCGCAAGACGGTGCGGGAGGTGGGGTACACC
CGGGCCAAGTACGGCTTTGACGCCGACACCTGCGCCGTCCTCACCGCCATAGACGAGCAGTCCCCCGACATCGCCGGCGG
GGTCAACCTCTCCTACGAGTGGCGGGTCCTCAAGTCCACCGACCCCCTGGACCGGGTGGGGGCCGGGGACCAGGGGCTCA
TGTTCGGTTACGCCACCGACGAGACCCCCGAGCTCATGCCCCTCCCCATCACCCTGGCCCACCGCCTCACCATGCGCCTC
GCCGAGGTGCGGAAGACGGGGCTTCTGCCCTACCTCCGGCCCGACGGCAAGGCCCAGGTCACCGTGGTCTACGAGGGGGA
CAAGCCCCTTTACGTCAAGACCGTGGTGGTCTCCGCCCAGCACTCCCCCGAGGTGGAGCAGGAGCAATTGCGGGAGGACC
TGATCCGGGAGGTGGTGCGCCAGGCCATCCCCCCGGAGTACCTGAAGGACGGGGAGACGGAGTACCTCATCAACCCCTCG
GGCCGCTTCATCCTGGGCGGGCCCCACGCGGACACGGGGCTTACGGGCCGGAAGATCATCGTGGACACCTATGGAGGGGC
GGTGCCCCACGGGGGCGGGGCCTTCAGCGGCAAGGACCCCACCAAGGTGGACCGCTCCGCCAGCTACTACGCCCGCTACA
TGGCGAAGAACATCGTGGCGGCGGGGCTTGCGAGGAGGGCCCTCGTGGAGCTCGCCTACGCCATCGGCAAGGCCAGGCCC
GTCTCCCTTAGGGTGGAGACCTTCGGCACCGGGGTCCTGCCCGACGAGAAGCTCACGGAGATCGCCAAGAAGGTCTTTGA
CCCGAGGCCCCTCGCCATCATTGAGGAGCTGGACCTCCTGCGGCCCATCTACACCCCCACCTCGGCCTACGGCCACTTCG
GCCGCCCGGGCTTCCCCTGGGAGGAGACGGACCGGGTGGAGGCCTTGAGGCGGGAAGCGGGGCTTTGA

Upstream 100 bases:

>100_bases
CTTGGGGTCCTTCACGGGGCCCCATCCTACCTCTCTGGACGGAAAGGGGGGGGTGCTATACTATCAGGGTTGCCCCGAAG
GGGGCATCGGGGTAAAGGAG

Downstream 100 bases:

>100_bases
GGCCACCCCATGCTGGCGCAAGCCAGCATGGGGGTAAGCGTCCGCGCGTCTAGAAAGACCCTTTTGGGGCCCCCACCGCG
GCGAAAGCCGCGGTGGGGCA

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MRALRLVTSESVTEGHPDKLADRISDAILDALIAQDKKARVAAETLVTTGLVFVAGEITTEGYVDIPNLVRKTVREVGYT
RAKYGFDADTCAVLTAIDEQSPDIAGGVNLSYEWRVLKSTDPLDRVGAGDQGLMFGYATDETPELMPLPITLAHRLTMRL
AEVRKTGLLPYLRPDGKAQVTVVYEGDKPLYVKTVVVSAQHSPEVEQEQLREDLIREVVRQAIPPEYLKDGETEYLINPS
GRFILGGPHADTGLTGRKIIVDTYGGAVPHGGGAFSGKDPTKVDRSASYYARYMAKNIVAAGLARRALVELAYAIGKARP
VSLRVETFGTGVLPDEKLTEIAKKVFDPRPLAIIEELDLLRPIYTPTSAYGHFGRPGFPWEETDRVEALRREAGL

Sequences:

>Translated_395_residues
MRALRLVTSESVTEGHPDKLADRISDAILDALIAQDKKARVAAETLVTTGLVFVAGEITTEGYVDIPNLVRKTVREVGYT
RAKYGFDADTCAVLTAIDEQSPDIAGGVNLSYEWRVLKSTDPLDRVGAGDQGLMFGYATDETPELMPLPITLAHRLTMRL
AEVRKTGLLPYLRPDGKAQVTVVYEGDKPLYVKTVVVSAQHSPEVEQEQLREDLIREVVRQAIPPEYLKDGETEYLINPS
GRFILGGPHADTGLTGRKIIVDTYGGAVPHGGGAFSGKDPTKVDRSASYYARYMAKNIVAAGLARRALVELAYAIGKARP
VSLRVETFGTGVLPDEKLTEIAKKVFDPRPLAIIEELDLLRPIYTPTSAYGHFGRPGFPWEETDRVEALRREAGL
>Mature_395_residues
MRALRLVTSESVTEGHPDKLADRISDAILDALIAQDKKARVAAETLVTTGLVFVAGEITTEGYVDIPNLVRKTVREVGYT
RAKYGFDADTCAVLTAIDEQSPDIAGGVNLSYEWRVLKSTDPLDRVGAGDQGLMFGYATDETPELMPLPITLAHRLTMRL
AEVRKTGLLPYLRPDGKAQVTVVYEGDKPLYVKTVVVSAQHSPEVEQEQLREDLIREVVRQAIPPEYLKDGETEYLINPS
GRFILGGPHADTGLTGRKIIVDTYGGAVPHGGGAFSGKDPTKVDRSASYYARYMAKNIVAAGLARRALVELAYAIGKARP
VSLRVETFGTGVLPDEKLTEIAKKVFDPRPLAIIEELDLLRPIYTPTSAYGHFGRPGFPWEETDRVEALRREAGL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI5174529, Length=380, Percent_Identity=54.4736842105263, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI4557737, Length=380, Percent_Identity=53.4210526315789, Blast_Score=398, Evalue=1e-111,
Organism=Escherichia coli, GI1789311, Length=393, Percent_Identity=59.5419847328244, Blast_Score=476, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI17538498, Length=381, Percent_Identity=54.0682414698163, Blast_Score=402, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17551082, Length=386, Percent_Identity=53.8860103626943, Blast_Score=401, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17538494, Length=397, Percent_Identity=52.1410579345088, Blast_Score=399, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI25145633, Length=386, Percent_Identity=52.8497409326425, Blast_Score=398, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI32565905, Length=397, Percent_Identity=48.3627204030227, Blast_Score=343, Evalue=9e-95,
Organism=Caenorhabditis elegans, GI32565911, Length=336, Percent_Identity=52.3809523809524, Blast_Score=340, Evalue=6e-94,
Organism=Caenorhabditis elegans, GI25145635, Length=341, Percent_Identity=51.0263929618768, Blast_Score=337, Evalue=9e-93,
Organism=Caenorhabditis elegans, GI32565903, Length=325, Percent_Identity=52, Blast_Score=312, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI32565909, Length=81, Percent_Identity=51.8518518518518, Blast_Score=96, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6320710, Length=386, Percent_Identity=54.4041450777202, Blast_Score=402, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6323209, Length=386, Percent_Identity=53.8860103626943, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI45552159, Length=389, Percent_Identity=51.413881748072, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580529, Length=389, Percent_Identity=51.413881748072, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580537, Length=389, Percent_Identity=51.413881748072, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580533, Length=389, Percent_Identity=51.413881748072, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580535, Length=389, Percent_Identity=51.413881748072, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580531, Length=389, Percent_Identity=51.413881748072, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580527, Length=389, Percent_Identity=51.413881748072, Blast_Score=371, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24580525, Length=389, Percent_Identity=51.413881748072, Blast_Score=371, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24580541, Length=388, Percent_Identity=49.2268041237113, Blast_Score=353, Evalue=1e-97,
Organism=Drosophila melanogaster, GI24580539, Length=129, Percent_Identity=49.6124031007752, Blast_Score=125, Evalue=6e-29,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_THET2 (Q72I53)

Other databases:

- EMBL:   AE017221
- RefSeq:   YP_005248.2
- ProteinModelPortal:   Q72I53
- SMR:   Q72I53
- STRING:   Q72I53
- GeneID:   2774677
- GenomeReviews:   AE017221_GR
- eggNOG:   COG0192
- HOGENOM:   HBG443662
- OMA:   RTAAYMA
- PhylomeDB:   Q72I53
- ProtClustDB:   PRK05250
- BioCyc:   TTHE262724:TT_C1279-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 43248; Mature: 43248

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRALRLVTSESVTEGHPDKLADRISDAILDALIAQDKKARVAAETLVTTGLVFVAGEITT
CCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEEEEEECC
EGYVDIPNLVRKTVREVGYTRAKYGFDADTCAVLTAIDEQSPDIAGGVNLSYEWRVLKST
CCCCCCHHHHHHHHHHHCCHHHHCCCCCCHHHEEEECCCCCCCCCCCCCCEEEEEEECCC
DPLDRVGAGDQGLMFGYATDETPELMPLPITLAHRLTMRLAEVRKTGLLPYLRPDGKAQV
CCHHHHCCCCCCEEEEECCCCCCCEEECCHHHHHHHHHHHHHHHHHCCCCEECCCCCEEE
TVVYEGDKPLYVKTVVVSAQHSPEVEQEQLREDLIREVVRQAIPPEYLKDGETEYLINPS
EEEEECCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEECCC
GRFILGGPHADTGLTGRKIIVDTYGGAVPHGGGAFSGKDPTKVDRSASYYARYMAKNIVA
CCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
AGLARRALVELAYAIGKARPVSLRVETFGTGVLPDEKLTEIAKKVFDPRPLAIIEELDLL
HHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHH
RPIYTPTSAYGHFGRPGFPWEETDRVEALRREAGL
HHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MRALRLVTSESVTEGHPDKLADRISDAILDALIAQDKKARVAAETLVTTGLVFVAGEITT
CCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEEEEEECC
EGYVDIPNLVRKTVREVGYTRAKYGFDADTCAVLTAIDEQSPDIAGGVNLSYEWRVLKST
CCCCCCHHHHHHHHHHHCCHHHHCCCCCCHHHEEEECCCCCCCCCCCCCCEEEEEEECCC
DPLDRVGAGDQGLMFGYATDETPELMPLPITLAHRLTMRLAEVRKTGLLPYLRPDGKAQV
CCHHHHCCCCCCEEEEECCCCCCCEEECCHHHHHHHHHHHHHHHHHCCCCEECCCCCEEE
TVVYEGDKPLYVKTVVVSAQHSPEVEQEQLREDLIREVVRQAIPPEYLKDGETEYLINPS
EEEEECCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEECCC
GRFILGGPHADTGLTGRKIIVDTYGGAVPHGGGAFSGKDPTKVDRSASYYARYMAKNIVA
CCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
AGLARRALVELAYAIGKARPVSLRVETFGTGVLPDEKLTEIAKKVFDPRPLAIIEELDLL
HHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHH
RPIYTPTSAYGHFGRPGFPWEETDRVEALRREAGL
HHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA