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The map label for this gene is gdhA [H]

Identifier: 55981545

GI number: 55981545

Start: 1498260

End: 1499534

Strand: Reverse

Name: gdhA [H]

Synonym: TTHA1576

Alternate gene names: 55981545

Gene position: 1499534-1498260 (Counterclockwise)

Preceding gene: 55981546

Following gene: 55981544

Centisome position: 81.07

GC content: 69.8

Gene sequence:

>1275_bases
ATGAAGAGCGAACCCCTTTCCTACCTGGGCAAGGACGGAGGCCCCTGGGAGATCTTCACGGAGCAGGTGGACCGGGTCGT
CCCCTACCTGGGGCGGCTCGCCCCCCTGGCGGAAAGCCTCAAGCGGCCCAAGAGAGTCCTCATCGTGGACGTCCCCGTGC
GTCTGGACGACGGGAGCGTGGCCTACTTTGAAGGGTACCGGGTCCACCACAACACCGCCCGGGGGCCCGCCAAGGGCGGG
GTGCGCTACCACCCCGAGGTCACCCTCTCCGAGGTCATGGCCCTGGCGGGGTGGATGACCATCAAAAACGCCGCCGTGGG
CCTGCCCTACGGGGGCGGCAAGGGCGGGATACGGGTGGACCCGAGGAAGCTTTCCCCCGGGGAGCTGGAGCGCCTCACCC
GCCGCTACACCTCGGAGATCGGGATCCTCCTCGGTCCCGACCGGGACATCCCCGCCCCCGACGTGAACACCGGGGAGCGG
GAGATGGCCTGGATGATGGACACCTACTCCATGAACGTGGGCCGCACCGTGCCCGGGGTGGTGACGGGGAAGCCCATCGC
CTTAGGCGGCTCCCTGGGCCGGAGGGACGCCACGGGGAGGGGGGTCTTCATCACCGCGGCGGCCGCGGCGGAGAAGATCG
GGCTTCAGGTGGAGGGGGCCAGGGTGGCCATCCAGGGCTTCGGCAACGTGGGCAACGCCGCCGCCCGGGCCTTCCACGAC
CACGGGGCCCGCGTCGTCGCCGTCCAGGACCACACCGGCACCGTCTACAACGAGGCGGGCATTGACCCTTACGACCTCCT
CCGCTACGTGCAGGAGTTCGGGGGGGTGAGGGGTTACCCCAAGGCGGAGCCCCTGCCGGCGGCGGACTTCTGGGGCCTGC
CCGTGGAGTTCCTCGTCCCGGCGGCCTTGGAGAAGCAGATCACCGAGCAGAACGCCTGGCGCATCCGGGCCCGGATCGTG
GCCGAGGGGGCGAACGGCCCCACCACCCCGGCCGCCGACGACATCCTCTTGGAGAAGGGGGTCCTGGTGGTCCCCGACGT
GATCGCCAACGCCGGGGGCGTGACGGTGAGCTACTTTGAGTGGGTCCAGGACTTCAACTCTTACTTCTGGACGGAGGAGG
AGATCAACGCCCGCCTGGAGCGGGTGCTCCGCAACGCCTTTGGGGCGGTGTGGCAGGTAGCTCAGGAGAAGAAGATCCCC
TTGCGCACCGCCGCCTACGTGGTGGCGGCGACCCGGGTCCTCGAGGCCCAGGCCCTCCGGGGCCTATACCCTTAG

Upstream 100 bases:

>100_bases
GCGGGGCGGGCTGGACCTGAGGATGGGCGCCTTGGCCCTCGCCTTGGAGCGGCTGGACGAGGCCACCCGGCTTCGGGGCG
TGTACCCGTGAGGAGCGGCG

Downstream 100 bases:

>100_bases
GCTATCCCCGTGGAATGGCCCCGCTTCGGCCGCGTGGTCCTCAAGCCCTTCTCCGCCGGGCTTTCCGAGGCGGAGTGGAA
GGGCCTCTACGAGACCTTCC

Product: NAD-dependent glutamate dehydrogenase

Products: NA

Alternate protein names: GDH [H]

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MKSEPLSYLGKDGGPWEIFTEQVDRVVPYLGRLAPLAESLKRPKRVLIVDVPVRLDDGSVAYFEGYRVHHNTARGPAKGG
VRYHPEVTLSEVMALAGWMTIKNAAVGLPYGGGKGGIRVDPRKLSPGELERLTRRYTSEIGILLGPDRDIPAPDVNTGER
EMAWMMDTYSMNVGRTVPGVVTGKPIALGGSLGRRDATGRGVFITAAAAAEKIGLQVEGARVAIQGFGNVGNAAARAFHD
HGARVVAVQDHTGTVYNEAGIDPYDLLRYVQEFGGVRGYPKAEPLPAADFWGLPVEFLVPAALEKQITEQNAWRIRARIV
AEGANGPTTPAADDILLEKGVLVVPDVIANAGGVTVSYFEWVQDFNSYFWTEEEINARLERVLRNAFGAVWQVAQEKKIP
LRTAAYVVAATRVLEAQALRGLYP

Sequences:

>Translated_424_residues
MKSEPLSYLGKDGGPWEIFTEQVDRVVPYLGRLAPLAESLKRPKRVLIVDVPVRLDDGSVAYFEGYRVHHNTARGPAKGG
VRYHPEVTLSEVMALAGWMTIKNAAVGLPYGGGKGGIRVDPRKLSPGELERLTRRYTSEIGILLGPDRDIPAPDVNTGER
EMAWMMDTYSMNVGRTVPGVVTGKPIALGGSLGRRDATGRGVFITAAAAAEKIGLQVEGARVAIQGFGNVGNAAARAFHD
HGARVVAVQDHTGTVYNEAGIDPYDLLRYVQEFGGVRGYPKAEPLPAADFWGLPVEFLVPAALEKQITEQNAWRIRARIV
AEGANGPTTPAADDILLEKGVLVVPDVIANAGGVTVSYFEWVQDFNSYFWTEEEINARLERVLRNAFGAVWQVAQEKKIP
LRTAAYVVAATRVLEAQALRGLYP
>Mature_424_residues
MKSEPLSYLGKDGGPWEIFTEQVDRVVPYLGRLAPLAESLKRPKRVLIVDVPVRLDDGSVAYFEGYRVHHNTARGPAKGG
VRYHPEVTLSEVMALAGWMTIKNAAVGLPYGGGKGGIRVDPRKLSPGELERLTRRYTSEIGILLGPDRDIPAPDVNTGER
EMAWMMDTYSMNVGRTVPGVVTGKPIALGGSLGRRDATGRGVFITAAAAAEKIGLQVEGARVAIQGFGNVGNAAARAFHD
HGARVVAVQDHTGTVYNEAGIDPYDLLRYVQEFGGVRGYPKAEPLPAADFWGLPVEFLVPAALEKQITEQNAWRIRARIV
AEGANGPTTPAADDILLEKGVLVVPDVIANAGGVTVSYFEWVQDFNSYFWTEEEINARLERVLRNAFGAVWQVAQEKKIP
LRTAAYVVAATRVLEAQALRGLYP

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=372, Percent_Identity=44.3548387096774, Blast_Score=311, Evalue=1e-84,
Organism=Homo sapiens, GI4885281, Length=372, Percent_Identity=43.8172043010753, Blast_Score=305, Evalue=6e-83,
Organism=Escherichia coli, GI1788059, Length=372, Percent_Identity=36.8279569892473, Blast_Score=204, Evalue=8e-54,
Organism=Caenorhabditis elegans, GI17544676, Length=362, Percent_Identity=43.0939226519337, Blast_Score=285, Evalue=3e-77,
Organism=Saccharomyces cerevisiae, GI6319256, Length=418, Percent_Identity=33.0143540669856, Blast_Score=159, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6324951, Length=384, Percent_Identity=32.03125, Blast_Score=152, Evalue=7e-38,
Organism=Drosophila melanogaster, GI45553475, Length=369, Percent_Identity=43.6314363143631, Blast_Score=293, Evalue=1e-79,
Organism=Drosophila melanogaster, GI45549226, Length=354, Percent_Identity=44.3502824858757, Blast_Score=293, Evalue=2e-79,
Organism=Drosophila melanogaster, GI24649283, Length=395, Percent_Identity=40.7594936708861, Blast_Score=286, Evalue=3e-77,
Organism=Drosophila melanogaster, GI24649460, Length=300, Percent_Identity=46, Blast_Score=251, Evalue=6e-67,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 46039; Mature: 46039

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSEPLSYLGKDGGPWEIFTEQVDRVVPYLGRLAPLAESLKRPKRVLIVDVPVRLDDGSV
CCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCE
AYFEGYRVHHNTARGPAKGGVRYHPEVTLSEVMALAGWMTIKNAAVGLPYGGGKGGIRVD
EEEECEEEECCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHCCEEECCCCCCCCCEEEC
PRKLSPGELERLTRRYTSEIGILLGPDRDIPAPDVNTGEREMAWMMDTYSMNVGRTVPGV
CCCCCCHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCE
VTGKPIALGGSLGRRDATGRGVFITAAAAAEKIGLQVEGARVAIQGFGNVGNAAARAFHD
ECCCCEEECCCCCCCCCCCCEEEEEEHHHHHHHCEEECCCEEEEEECCCCCHHHHHHHHH
HGARVVAVQDHTGTVYNEAGIDPYDLLRYVQEFGGVRGYPKAEPLPAADFWGLPVEFLVP
CCCEEEEEECCCCCEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHH
AALEKQITEQNAWRIRARIVAEGANGPTTPAADDILLEKGVLVVPDVIANAGGVTVSYFE
HHHHHHHHHCCCEEEEEEEEEECCCCCCCCCCCHHHHHCCCEEECHHHCCCCCCCHHHHH
WVQDFNSYFWTEEEINARLERVLRNAFGAVWQVAQEKKIPLRTAAYVVAATRVLEAQALR
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
GLYP
CCCC
>Mature Secondary Structure
MKSEPLSYLGKDGGPWEIFTEQVDRVVPYLGRLAPLAESLKRPKRVLIVDVPVRLDDGSV
CCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCE
AYFEGYRVHHNTARGPAKGGVRYHPEVTLSEVMALAGWMTIKNAAVGLPYGGGKGGIRVD
EEEECEEEECCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHCCEEECCCCCCCCCEEEC
PRKLSPGELERLTRRYTSEIGILLGPDRDIPAPDVNTGEREMAWMMDTYSMNVGRTVPGV
CCCCCCHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCE
VTGKPIALGGSLGRRDATGRGVFITAAAAAEKIGLQVEGARVAIQGFGNVGNAAARAFHD
ECCCCEEECCCCCCCCCCCCEEEEEEHHHHHHHCEEECCCEEEEEECCCCCHHHHHHHHH
HGARVVAVQDHTGTVYNEAGIDPYDLLRYVQEFGGVRGYPKAEPLPAADFWGLPVEFLVP
CCCEEEEEECCCCCEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHH
AALEKQITEQNAWRIRARIVAEGANGPTTPAADDILLEKGVLVVPDVIANAGGVTVSYFE
HHHHHHHHHCCCEEEEEEEEEECCCCCCCCCCCHHHHHCCCEEECHHHCCCCCCCHHHHH
WVQDFNSYFWTEEEINARLERVLRNAFGAVWQVAQEKKIPLRTAAYVVAATRVLEAQALR
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
GLYP
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9680336; 10360571; 9135121; 9654452; 10366510 [H]