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The map label for this gene is nplT [H]

Identifier: 55981532

GI number: 55981532

Start: 1486211

End: 1487638

Strand: Direct

Name: nplT [H]

Synonym: TTHA1563

Alternate gene names: 55981532

Gene position: 1486211-1487638 (Clockwise)

Preceding gene: 55981526

Following gene: 55981551

Centisome position: 80.35

GC content: 69.33

Gene sequence:

>1428_bases
ATGGCATGGTACGAAGGCGCCTTCTTTTACCAGATCTTCCCCGACCGCTTCTTCCGCGCAGGCCCGCCGGGCAGGCCCGC
CCCCGCGGGGCCCTTTGAGCCCTGGGAAGCCCCCCCTACCCTCAGGGGCTTCAAAGGAGGGACGCTTTGGGGCGTGGCGG
AGAAGCTCCCCTACCTCCTGGACCTGGGGGTGGAGGCCATCTACCTCAACCCCGTCTTCGCCTCCACCGCCAACCACCGC
TACCACACCGTGGACTACTTCCAGGTAGACCCCATCCTGGGGGGAAACGAGGCCTTAAGGCACCTCCTGGAGGTGGCCCA
CGCCCACGGGGTGAGGGTCATCCTGGACGGGGTCTTCAACCACACGGGCCGGGGCTTCTTCGCCTTCCAGCACCTCATGG
AAAACGGCGAGCAAAGCCCCTACCGGGACTGGTACCACGTCAAGGGCTTCCCCCTAAAGGCCTACACCGCCCACCCCAAC
TACGAGGCCTGGTGGGGCAACCCCGAGCTCCCCAAGCTCAAGGTGGAGACCCCGGCGGTGCGGGAATACCTCCTCGCCGT
GGCCGAGCACTGGATCCGCTTCGGCGTAGACGGCTGGCGTCTGGACGTGCCCAACGAAATCCCCGACCCCACCTTCTGGC
GGGAGTTCCGCCAAAGGGTCAAGGGGGCGAACCCCGAGGCCTACATCGTGGGGGAGATCTGGGAGGAGGCGGACTTTTGG
CTCCAGGGGGACATGTTTGACGCGGTGATGAACTACCCCCTCGCCCGGGCCGTCCTGGGCTTCGTGGGCGGGGAGGCCCT
GGACCGGGACCTCGCGGCGCAAACGGGCCTAGGCCGCATAGAACCCCTCCAGGCCCTGGCCTTCAGCCATCGGCTGGAGG
ACCTCTTCGGCCGCTACCGCCCCGAGGTGGTCCGGGCCCAGATGAACCTCCTCACCTCCCACGACACCCCCAGGCTCCTC
AGCCTGATGCGGGGGAGCGTGGAGCGGGCCCGGCTGGCCCTGGCCCTCCTCTTCCTCCTCCCGGGGAACCCCACCGTCTA
CTACGGGGAGGAAGTGGGGATGGCGGGGGGCAAGGACCCGGAAAACCGGGGCGGGATGGTCTGGGAGGAGGCCCGCTGGC
AGAAGGACCTCCGGGAGACGGTCAAGCGCCTGGCCCGGCTCAGGAAGGAACACCCCGCGCTCCGCACCGCCCCCTACCTG
CGGATCTACGCCCAGGACGGCCACCTGGCCTTCGCCCGGGGGCCCTACCTGGCGGTGGTGAACGCCTCTCCCCACCCCTT
CCGCCAGGACTTCCCCCTGCACGGGGTCTTCCCCCGGGGGGGAAGGGCGGTGGACCTCCTCTCCGGGGAGGTCTGCACGC
CCCAGGGGGGGAGGCTCTGCGGCCCCGTGCTTCCGCCCTTCTCCCTGGCCCTGTGGCGGGAGGCCTAA

Upstream 100 bases:

>100_bases
CTTGCCCACCCCGCTCGCCCCGGTCATCACGAAAAGGCGGCCCCGCATCCCCTTCACCCGGGAGAGGATACCACAGGACC
GGGGTGTTAGACTCGGAGGC

Downstream 100 bases:

>100_bases
AGGGCCCCCTCAAAGCGGAGGAGCCAAAGCTTCAGCCCCTCCTGCCCCTGAAACCCCCCAAGCCGCCCGTCGGCGTGGAT
CACCCGGTGGGCGGGGACGA

Product: (neo)pullulanase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MAWYEGAFFYQIFPDRFFRAGPPGRPAPAGPFEPWEAPPTLRGFKGGTLWGVAEKLPYLLDLGVEAIYLNPVFASTANHR
YHTVDYFQVDPILGGNEALRHLLEVAHAHGVRVILDGVFNHTGRGFFAFQHLMENGEQSPYRDWYHVKGFPLKAYTAHPN
YEAWWGNPELPKLKVETPAVREYLLAVAEHWIRFGVDGWRLDVPNEIPDPTFWREFRQRVKGANPEAYIVGEIWEEADFW
LQGDMFDAVMNYPLARAVLGFVGGEALDRDLAAQTGLGRIEPLQALAFSHRLEDLFGRYRPEVVRAQMNLLTSHDTPRLL
SLMRGSVERARLALALLFLLPGNPTVYYGEEVGMAGGKDPENRGGMVWEEARWQKDLRETVKRLARLRKEHPALRTAPYL
RIYAQDGHLAFARGPYLAVVNASPHPFRQDFPLHGVFPRGGRAVDLLSGEVCTPQGGRLCGPVLPPFSLALWREA

Sequences:

>Translated_475_residues
MAWYEGAFFYQIFPDRFFRAGPPGRPAPAGPFEPWEAPPTLRGFKGGTLWGVAEKLPYLLDLGVEAIYLNPVFASTANHR
YHTVDYFQVDPILGGNEALRHLLEVAHAHGVRVILDGVFNHTGRGFFAFQHLMENGEQSPYRDWYHVKGFPLKAYTAHPN
YEAWWGNPELPKLKVETPAVREYLLAVAEHWIRFGVDGWRLDVPNEIPDPTFWREFRQRVKGANPEAYIVGEIWEEADFW
LQGDMFDAVMNYPLARAVLGFVGGEALDRDLAAQTGLGRIEPLQALAFSHRLEDLFGRYRPEVVRAQMNLLTSHDTPRLL
SLMRGSVERARLALALLFLLPGNPTVYYGEEVGMAGGKDPENRGGMVWEEARWQKDLRETVKRLARLRKEHPALRTAPYL
RIYAQDGHLAFARGPYLAVVNASPHPFRQDFPLHGVFPRGGRAVDLLSGEVCTPQGGRLCGPVLPPFSLALWREA
>Mature_474_residues
AWYEGAFFYQIFPDRFFRAGPPGRPAPAGPFEPWEAPPTLRGFKGGTLWGVAEKLPYLLDLGVEAIYLNPVFASTANHRY
HTVDYFQVDPILGGNEALRHLLEVAHAHGVRVILDGVFNHTGRGFFAFQHLMENGEQSPYRDWYHVKGFPLKAYTAHPNY
EAWWGNPELPKLKVETPAVREYLLAVAEHWIRFGVDGWRLDVPNEIPDPTFWREFRQRVKGANPEAYIVGEIWEEADFWL
QGDMFDAVMNYPLARAVLGFVGGEALDRDLAAQTGLGRIEPLQALAFSHRLEDLFGRYRPEVVRAQMNLLTSHDTPRLLS
LMRGSVERARLALALLFLLPGNPTVYYGEEVGMAGGKDPENRGGMVWEEARWQKDLRETVKRLARLRKEHPALRTAPYLR
IYAQDGHLAFARGPYLAVVNASPHPFRQDFPLHGVFPRGGRAVDLLSGEVCTPQGGRLCGPVLPPFSLALWREA

Specific function: Hydrolyzes pullulan efficiently but only a small amount of starch. Endohydrolysis of 1,4-alpha-glucosidic linkages in pullulan to form panose. Cleaves also (1-6)-alpha-glucosidic linkages to form maltotriose [H]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=400, Percent_Identity=24.25, Blast_Score=90, Evalue=5e-18,
Organism=Escherichia coli, GI1786604, Length=450, Percent_Identity=35.1111111111111, Blast_Score=215, Evalue=4e-57,
Organism=Escherichia coli, GI1790687, Length=399, Percent_Identity=27.8195488721804, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1789995, Length=516, Percent_Identity=23.4496124031008, Blast_Score=98, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6321726, Length=424, Percent_Identity=25.2358490566038, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6322245, Length=225, Percent_Identity=27.5555555555556, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6322241, Length=227, Percent_Identity=26.8722466960352, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6322021, Length=227, Percent_Identity=26.8722466960352, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324416, Length=227, Percent_Identity=26.8722466960352, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24586593, Length=413, Percent_Identity=27.1186440677966, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24586587, Length=428, Percent_Identity=23.3644859813084, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24586591, Length=406, Percent_Identity=26.1083743842365, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24586589, Length=408, Percent_Identity=25, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24586599, Length=413, Percent_Identity=25.4237288135593, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24583747, Length=229, Percent_Identity=26.2008733624454, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24583749, Length=229, Percent_Identity=26.2008733624454, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI221330053, Length=223, Percent_Identity=26.457399103139, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45549022, Length=223, Percent_Identity=26.9058295964126, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N [H]

EC number: =3.2.1.135 [H]

Molecular weight: Translated: 53759; Mature: 53628

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAWYEGAFFYQIFPDRFFRAGPPGRPAPAGPFEPWEAPPTLRGFKGGTLWGVAEKLPYLL
CCEECCEEEEEECCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHH
DLGVEAIYLNPVFASTANHRYHTVDYFQVDPILGGNEALRHLLEVAHAHGVRVILDGVFN
HCCCEEEEECCHHHCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEHHHC
HTGRGFFAFQHLMENGEQSPYRDWYHVKGFPLKAYTAHPNYEAWWGNPELPKLKVETPAV
CCCCHHHHHHHHHHCCCCCCCCCCEEECCCCEEEEECCCCCCEECCCCCCCEEEECCHHH
REYLLAVAEHWIRFGVDGWRLDVPNEIPDPTFWREFRQRVKGANPEAYIVGEIWEEADFW
HHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEHHCCCCCE
LQGDMFDAVMNYPLARAVLGFVGGEALDRDLAAQTGLGRIEPLQALAFSHRLEDLFGRYR
EECHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHC
PEVVRAQMNLLTSHDTPRLLSLMRGSVERARLALALLFLLPGNPTVYYGEEVGMAGGKDP
HHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCC
ENRGGMVWEEARWQKDLRETVKRLARLRKEHPALRTAPYLRIYAQDGHLAFARGPYLAVV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEECCCEEEEE
NASPHPFRQDFPLHGVFPRGGRAVDLLSGEVCTPQGGRLCGPVLPPFSLALWREA
CCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHCCCC
>Mature Secondary Structure 
AWYEGAFFYQIFPDRFFRAGPPGRPAPAGPFEPWEAPPTLRGFKGGTLWGVAEKLPYLL
CEECCEEEEEECCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHH
DLGVEAIYLNPVFASTANHRYHTVDYFQVDPILGGNEALRHLLEVAHAHGVRVILDGVFN
HCCCEEEEECCHHHCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEHHHC
HTGRGFFAFQHLMENGEQSPYRDWYHVKGFPLKAYTAHPNYEAWWGNPELPKLKVETPAV
CCCCHHHHHHHHHHCCCCCCCCCCEEECCCCEEEEECCCCCCEECCCCCCCEEEECCHHH
REYLLAVAEHWIRFGVDGWRLDVPNEIPDPTFWREFRQRVKGANPEAYIVGEIWEEADFW
HHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEHHCCCCCE
LQGDMFDAVMNYPLARAVLGFVGGEALDRDLAAQTGLGRIEPLQALAFSHRLEDLFGRYR
EECHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHC
PEVVRAQMNLLTSHDTPRLLSLMRGSVERARLALALLFLLPGNPTVYYGEEVGMAGGKDP
HHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCC
ENRGGMVWEEARWQKDLRETVKRLARLRKEHPALRTAPYLRIYAQDGHLAFARGPYLAVV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEECCCEEEEE
NASPHPFRQDFPLHGVFPRGGRAVDLLSGEVCTPQGGRLCGPVLPPFSLALWREA
CCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2482332; 12547200 [H]