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The map label for this gene is purU [H]

Identifier: 55981290

GI number: 55981290

Start: 1258888

End: 1259745

Strand: Reverse

Name: purU [H]

Synonym: TTHA1321

Alternate gene names: 55981290

Gene position: 1259745-1258888 (Counterclockwise)

Preceding gene: 55981292

Following gene: 55981289

Centisome position: 68.1

GC content: 67.95

Gene sequence:

>858_bases
ATGGAGGAGGCGAGGCTTCTCGTCACCTGTCCGGACCGGCCCGGCATCGTGGCCGCCGTGAGCGGTTTCCTCTACGCCCA
TGGGGCCAACATCACCGATCTGCAGCAGCACTCCACCGACCCCGAGGGGGGGACCTTCTTCATGCGGGTGGCCTTCACCG
CCTCCCACCTGGACCTGGCCCGCCCCGCCCTGGAAAGGGCCTTCCAGGAGGTGGTGGCGAGCCGCTTCCAGATGCAGTGG
CGCCTGGCTTACGCCTCCGAGAGGAAGCGGACGGCCATCCTCGTCTCCAAGCCCGCCCACGCCCTCTTGGAGCTCCTTTG
GCGCTACCGGGTGGGAGAGCTTCCCATGGAGCTCCGCCTGGTCATCTCCAACCACCCCGACCACCGGGAGGAGGTGGAGC
GCTTCGGCATCCCCTACCACCACGTGCCCGTGGAGAAGGGCAGGAAGGAGGAGGCGGAGGAGAGGATCCTCGCCCTTTTG
GAGGCGGAGGGGGTGGAGCTTGTGGTCCTCGCCCGCTACATGCAGATCCTCTCCCCCGGCTTCGTGGAGCGCTTCCCCAT
GCGCATCATCAACATCCACCACTCCTTCCTTCCCGCCTTCGCCGGGGCCGACCCCTACCGCCAGGCCTACGAGCGGGGCG
TGAAGCTCATCGGGGCCACGGCCCACTACGTCACCGAGGAGCTGGACCAGGGCCCCATCATTGAGCAGGACGTGGTGCGG
GTCTCCCACCGCCACTCGGTGCGGGAGATGAAGCGCCTTGGGCGGGAGCTGGAACGCACCGTCTTGGCCCGGGCGGTGCG
CTGGCACCTGGAGGACCGGATCCTGGTCCACGAGAACCGCACCGTGGTCTTCGTCTAA

Upstream 100 bases:

>100_bases
TTCCGCCGAAGCTTTTGAAGACCATGGGGAGGGCCTCGCCCTCCGGGTAAAGGTCGGAGAGGTCCGTGGTGCGCGCCTCC
ATGGGGATATAGTAGCCCCC

Downstream 100 bases:

>100_bases
TGGCGGGCTAACCGGGGGGGCGTAGGGTGGAACTGGGAGGTGTGGCATGAACCTGGGACGTTCCTTTGTGGGCTTTCTGG
TGCTCTCCCTGATGGCGGGG

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MEEARLLVTCPDRPGIVAAVSGFLYAHGANITDLQQHSTDPEGGTFFMRVAFTASHLDLARPALERAFQEVVASRFQMQW
RLAYASERKRTAILVSKPAHALLELLWRYRVGELPMELRLVISNHPDHREEVERFGIPYHHVPVEKGRKEEAEERILALL
EAEGVELVVLARYMQILSPGFVERFPMRIINIHHSFLPAFAGADPYRQAYERGVKLIGATAHYVTEELDQGPIIEQDVVR
VSHRHSVREMKRLGRELERTVLARAVRWHLEDRILVHENRTVVFV

Sequences:

>Translated_285_residues
MEEARLLVTCPDRPGIVAAVSGFLYAHGANITDLQQHSTDPEGGTFFMRVAFTASHLDLARPALERAFQEVVASRFQMQW
RLAYASERKRTAILVSKPAHALLELLWRYRVGELPMELRLVISNHPDHREEVERFGIPYHHVPVEKGRKEEAEERILALL
EAEGVELVVLARYMQILSPGFVERFPMRIINIHHSFLPAFAGADPYRQAYERGVKLIGATAHYVTEELDQGPIIEQDVVR
VSHRHSVREMKRLGRELERTVLARAVRWHLEDRILVHENRTVVFV
>Mature_285_residues
MEEARLLVTCPDRPGIVAAVSGFLYAHGANITDLQQHSTDPEGGTFFMRVAFTASHLDLARPALERAFQEVVASRFQMQW
RLAYASERKRTAILVSKPAHALLELLWRYRVGELPMELRLVISNHPDHREEVERFGIPYHHVPVEKGRKEEAEERILALL
EAEGVELVVLARYMQILSPGFVERFPMRIINIHHSFLPAFAGADPYRQAYERGVKLIGATAHYVTEELDQGPIIEQDVVR
VSHRHSVREMKRLGRELERTVLARAVRWHLEDRILVHENRTVVFV

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=208, Percent_Identity=29.8076923076923, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI209869995, Length=208, Percent_Identity=29.8076923076923, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI209869993, Length=208, Percent_Identity=29.8076923076923, Blast_Score=93, Evalue=3e-19,
Organism=Escherichia coli, GI1787483, Length=280, Percent_Identity=43.5714285714286, Blast_Score=222, Evalue=3e-59,
Organism=Escherichia coli, GI1788846, Length=190, Percent_Identity=31.0526315789474, Blast_Score=93, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17567511, Length=180, Percent_Identity=36.1111111111111, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24582400, Length=162, Percent_Identity=32.7160493827161, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32786; Mature: 32786

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEARLLVTCPDRPGIVAAVSGFLYAHGANITDLQQHSTDPEGGTFFMRVAFTASHLDLA
CCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHCCCCCCCCEEEEEEEEHHHHHHHH
RPALERAFQEVVASRFQMQWRLAYASERKRTAILVSKPAHALLELLWRYRVGELPMELRL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCEEEE
VISNHPDHREEVERFGIPYHHVPVEKGRKEEAEERILALLEAEGVELVVLARYMQILSPG
EECCCCCHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCH
FVERFPMRIINIHHSFLPAFAGADPYRQAYERGVKLIGATAHYVTEELDQGPIIEQDVVR
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHH
VSHRHSVREMKRLGRELERTVLARAVRWHLEDRILVHENRTVVFV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEC
>Mature Secondary Structure
MEEARLLVTCPDRPGIVAAVSGFLYAHGANITDLQQHSTDPEGGTFFMRVAFTASHLDLA
CCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHCCCCCCCCEEEEEEEEHHHHHHHH
RPALERAFQEVVASRFQMQWRLAYASERKRTAILVSKPAHALLELLWRYRVGELPMELRL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCEEEE
VISNHPDHREEVERFGIPYHHVPVEKGRKEEAEERILALLEAEGVELVVLARYMQILSPG
EECCCCCHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCH
FVERFPMRIINIHHSFLPAFAGADPYRQAYERGVKLIGATAHYVTEELDQGPIIEQDVVR
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHH
VSHRHSVREMKRLGRELERTVLARAVRWHLEDRILVHENRTVVFV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]