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The map label for this gene is pcrA [H]

Identifier: 55980967

GI number: 55980967

Start: 942364

End: 944937

Strand: Reverse

Name: pcrA [H]

Synonym: TTHA0998

Alternate gene names: 55980967

Gene position: 944937-942364 (Counterclockwise)

Preceding gene: 55980968

Following gene: 55980965

Centisome position: 51.08

GC content: 74.09

Gene sequence:

>2574_bases
GTGAAGCTCTACGTGGCCTCGGCGGGCACGGGGAAGACCCATACCCTGGTGGAGGAGCTTTTGGCCCTGGTGAAGGGGGG
TGTGCCCCTGCGGCGCATCGCCGCCGTCACCTTCACCCGCAAGGCGGCGGAGGAGCTTCGGGCCCGGGCGAGGAAGGGGG
TGGAGGCCCTGGCCCGGGAGGAGCCGGGCCTTGGCGGGGCGGTCCGGGAGGTCCACGGGGCGGTCTTCGGCACCATCCAC
GGCTTCATGGCGGAGGCCTTGCGCCACGCGGCCCCCTTCCTCTCCCTGGACCCGGACTTCGCCGTGGTGGACGAGCTCCT
CGCCGAGGCCCTCTTCCTGGAGGAGGCGCGAAGCCTCCTCTACCTCCTGGGGGAGGACCCGGGGCTTGAGGCCTTCCTCC
GCCGCCTCTACCAGGGGCGGACCCTGGCCGAGGCGTTTAGGCCTCTGGAGGGGGCGGAGGGGCTTGTGCGCCTCTTCGCG
GAGGCCCTGGCCCGGTACCGCCGCCGGGCCCAGGAGGTCCTGGGCCCGGGGGACCTCGAGGCCCTCGCCCTCCGCCTCCT
CCGCCACCCCCAGGCCCTCGCCCGGGTGGTGGAGCGCTTTCCCCACGTCCTCGTGGACGAGTACCAGGACACGAGCCCCC
TCCAGGGGCGCTTCTTCCGGGCCCTGGAGGAGGCGGGGGCCCGGGTGGTGGCCGTGGGGGACCCGAAGCAGTCCATCTAC
CTCTTCCGCAACGCCCGGCTGGAGGTCTTCCGCGAGGCCCTGGGGCGGGCGGAGGAAGTCTTGGTCCTGGACGAGACCCG
CCGCCACGCCCGGAGGGTGGCCGCTTTCCTGAACCGCTTCGTGGCCCTCTTCCCCGAGGGGGAGCGGGTGGCGGTGCGCC
CCACCAGGGAAGCCGAGGGCCGGGTGGAGGTCCACTGGGTGGAGGGGGGGGCGGGGCTTGAGGAGCGGAGGCGGTTTGAG
GCCGACCTCCTCGCCCGCCGCCTCCTCGCCCTAAAGGCGGAGGGCTACGCCTTCGGGGAGATGGCCGTCTTGGTGCGGAG
CCGCGCGAGCCTTCCCCTTTTGGAGCGGGCCTTCCGCGCCCGGGGCGTGCCTTACGTCCTTCGCCGGGGGCAGAGCTTCT
TCAACCGCCTCGAGGTCCGGGACCTTTACCACGCCCTGCGGCTCGCCCTCCTCGAGGGGCCCCCGGGGCCCGAGGAGCGC
CGCTCCCTCCTCGCCTTTCTGCGAAGCCCCTTCGTGGGGCTCAACCTTGGGGAGGTGGAGGAGGCCCTCCTCCGGGAAGA
CCCGCTTCCTTACCTCCCCCAGGCGGTGCGGGACCGGGTGGCCTGGCTGAGGGGCCTCGCCGGGAAGCGGCCCCTCGAGG
CCCTCAAGGCCCTGGTGCGGGACGAGGTCTTTCTTTCTCGGCTTTCCCCCCGGGCCCGGGCCAACCTGGACGCCCTCCTC
CTCCTTGCGGGCCTGGAGCGCTTCCCCGACCTGGAGGCCCTCCTGGAGTGGCTCAGGCTCCGGGCCCTGGACCCCGAGGC
GAGCGAGCTTCCCGAGGGTGGGGAAGGGGTGGGCCTCCTCACGGTCCACGCCGCCAAGGGGCTGGAGTGGCCGGTGGTGG
CCGTCTACGACGTGGCCCGAGGGGAGCCAGGCCCGGCCTCCCCGGTCCTCGTGGGGCCTGAGGGGGAGGTGGCCGTGGCG
GGGACGCGGGCCCACCGGGACCTCGCCCAAAGGCTTGCTCAGGCCCAGAGGGAGGAGGCGGTCCGCCTCCTCTACGTGGC
CCTCTCCCGGGCCCGGGACGTCCTCCTCGTCACCGGAAGCCGCTCGGGCCGGGAAAGCCCCTGGGCCCAGGCCCTGGAAA
GGCTCGGCCTGGGCCCGGAAAGCCAGGACCCCCTGGTGGCGCGCCACCCTGTGCCCCAGGGCTGGAGGCCGCCCCGTCCT
GCGCCCGCCGCGAGGCGGGAAGGGCCTCCAGCCCCCTACGCCGAGCACCTCCTTCCCCCAAGGCCCCTCCCCCTCGTCCA
CTCCCCGAGCGCCCACCTGAAGGCGGAGCCCAAGCCCCTCGCCGAGGCCTTGGAGGGGGAGGCCGTCTCCGGGTTCGCCC
GCGCCCTCGGCACCCTGGTCCACTACGCCCTCGCCCGGGACCTGGACCCGGAGGACGGGAGGGCCATGCGGGCCCTCCTC
CACCAGGAGGTGGCCTTTCCCTTCGCCGAGGGGGAGAAGGAGCGGCTTTTGGCCGAGGTCCGGGCCCTGCTTCGGCGCTA
CCGGGGGATGCTGGGGCGGGAGCTTCCCGCTTTGGAGGAGCGGGCGGAGGATTACCGGGAGCTTCCCCTCGTCCTCCCCC
TCAAGGGGACGGTCTGGCACGGGGTGCTGGACCGGGTCTACCGGGTGGGGGACCGCTGGTACCTGGACGACTACAAGACG
GACCGGGAGGTCCGGCCCGAGGCCTACCGCCTCCAGCTCGCCCTCTACCGGCGGGCCCTCCTCGAGGCCTGGGGGGTGAG
GGCCGAGGCCCGGCTCGTCTACCTGCGCGCCGGGCGGGTCCACGCCTTCGGCGAGGCGGAGCTTGAGGAGGCCTTGGCGG
CCCTCCCCTCATAG

Upstream 100 bases:

>100_bases
TTCTGGAGAAGGCCCTCCGGTCCTGGGGCGCCTTTCCCCCGAGGCCCGGGCCCCACTGCCACGCCTGCGGCCTCGCCGAC
CTTTGCCGGAAGGAGGAGGA

Downstream 100 bases:

>100_bases
CTCCAGGTGCTGGGCGTCGTGGCGGCCTCCCCAGAGGCGCTCCTCTCCCTCGGGGGTGAGGAGGAAGCTGTTGCGGTCGT
CCCGGACCTGGCGCTTGAGC

Product: ATP-dependent DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 857; Mature: 857

Protein sequence:

>857_residues
MKLYVASAGTGKTHTLVEELLALVKGGVPLRRIAAVTFTRKAAEELRARARKGVEALAREEPGLGGAVREVHGAVFGTIH
GFMAEALRHAAPFLSLDPDFAVVDELLAEALFLEEARSLLYLLGEDPGLEAFLRRLYQGRTLAEAFRPLEGAEGLVRLFA
EALARYRRRAQEVLGPGDLEALALRLLRHPQALARVVERFPHVLVDEYQDTSPLQGRFFRALEEAGARVVAVGDPKQSIY
LFRNARLEVFREALGRAEEVLVLDETRRHARRVAAFLNRFVALFPEGERVAVRPTREAEGRVEVHWVEGGAGLEERRRFE
ADLLARRLLALKAEGYAFGEMAVLVRSRASLPLLERAFRARGVPYVLRRGQSFFNRLEVRDLYHALRLALLEGPPGPEER
RSLLAFLRSPFVGLNLGEVEEALLREDPLPYLPQAVRDRVAWLRGLAGKRPLEALKALVRDEVFLSRLSPRARANLDALL
LLAGLERFPDLEALLEWLRLRALDPEASELPEGGEGVGLLTVHAAKGLEWPVVAVYDVARGEPGPASPVLVGPEGEVAVA
GTRAHRDLAQRLAQAQREEAVRLLYVALSRARDVLLVTGSRSGRESPWAQALERLGLGPESQDPLVARHPVPQGWRPPRP
APAARREGPPAPYAEHLLPPRPLPLVHSPSAHLKAEPKPLAEALEGEAVSGFARALGTLVHYALARDLDPEDGRAMRALL
HQEVAFPFAEGEKERLLAEVRALLRRYRGMLGRELPALEERAEDYRELPLVLPLKGTVWHGVLDRVYRVGDRWYLDDYKT
DREVRPEAYRLQLALYRRALLEAWGVRAEARLVYLRAGRVHAFGEAELEEALAALPS

Sequences:

>Translated_857_residues
MKLYVASAGTGKTHTLVEELLALVKGGVPLRRIAAVTFTRKAAEELRARARKGVEALAREEPGLGGAVREVHGAVFGTIH
GFMAEALRHAAPFLSLDPDFAVVDELLAEALFLEEARSLLYLLGEDPGLEAFLRRLYQGRTLAEAFRPLEGAEGLVRLFA
EALARYRRRAQEVLGPGDLEALALRLLRHPQALARVVERFPHVLVDEYQDTSPLQGRFFRALEEAGARVVAVGDPKQSIY
LFRNARLEVFREALGRAEEVLVLDETRRHARRVAAFLNRFVALFPEGERVAVRPTREAEGRVEVHWVEGGAGLEERRRFE
ADLLARRLLALKAEGYAFGEMAVLVRSRASLPLLERAFRARGVPYVLRRGQSFFNRLEVRDLYHALRLALLEGPPGPEER
RSLLAFLRSPFVGLNLGEVEEALLREDPLPYLPQAVRDRVAWLRGLAGKRPLEALKALVRDEVFLSRLSPRARANLDALL
LLAGLERFPDLEALLEWLRLRALDPEASELPEGGEGVGLLTVHAAKGLEWPVVAVYDVARGEPGPASPVLVGPEGEVAVA
GTRAHRDLAQRLAQAQREEAVRLLYVALSRARDVLLVTGSRSGRESPWAQALERLGLGPESQDPLVARHPVPQGWRPPRP
APAARREGPPAPYAEHLLPPRPLPLVHSPSAHLKAEPKPLAEALEGEAVSGFARALGTLVHYALARDLDPEDGRAMRALL
HQEVAFPFAEGEKERLLAEVRALLRRYRGMLGRELPALEERAEDYRELPLVLPLKGTVWHGVLDRVYRVGDRWYLDDYKT
DREVRPEAYRLQLALYRRALLEAWGVRAEARLVYLRAGRVHAFGEAELEEALAALPS
>Mature_857_residues
MKLYVASAGTGKTHTLVEELLALVKGGVPLRRIAAVTFTRKAAEELRARARKGVEALAREEPGLGGAVREVHGAVFGTIH
GFMAEALRHAAPFLSLDPDFAVVDELLAEALFLEEARSLLYLLGEDPGLEAFLRRLYQGRTLAEAFRPLEGAEGLVRLFA
EALARYRRRAQEVLGPGDLEALALRLLRHPQALARVVERFPHVLVDEYQDTSPLQGRFFRALEEAGARVVAVGDPKQSIY
LFRNARLEVFREALGRAEEVLVLDETRRHARRVAAFLNRFVALFPEGERVAVRPTREAEGRVEVHWVEGGAGLEERRRFE
ADLLARRLLALKAEGYAFGEMAVLVRSRASLPLLERAFRARGVPYVLRRGQSFFNRLEVRDLYHALRLALLEGPPGPEER
RSLLAFLRSPFVGLNLGEVEEALLREDPLPYLPQAVRDRVAWLRGLAGKRPLEALKALVRDEVFLSRLSPRARANLDALL
LLAGLERFPDLEALLEWLRLRALDPEASELPEGGEGVGLLTVHAAKGLEWPVVAVYDVARGEPGPASPVLVGPEGEVAVA
GTRAHRDLAQRLAQAQREEAVRLLYVALSRARDVLLVTGSRSGRESPWAQALERLGLGPESQDPLVARHPVPQGWRPPRP
APAARREGPPAPYAEHLLPPRPLPLVHSPSAHLKAEPKPLAEALEGEAVSGFARALGTLVHYALARDLDPEDGRAMRALL
HQEVAFPFAEGEKERLLAEVRALLRRYRGMLGRELPALEERAEDYRELPLVLPLKGTVWHGVLDRVYRVGDRWYLDDYKT
DREVRPEAYRLQLALYRRALLEAWGVRAEARLVYLRAGRVHAFGEAELEEALAALPS

Specific function: Has Both Atpase And Helicase Activities. Unwinds DNA Duplexes With 3' To 5' Polarity With Respect To The Bound Strand And Initiates Unwinding Most Effectively When A Single-Stranded Region Is Present. Involved In The Post-Incision Events Of Nucleotide Exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=605, Percent_Identity=26.7768595041322, Blast_Score=119, Evalue=7e-28,
Organism=Escherichia coli, GI48994965, Length=631, Percent_Identity=26.3074484944533, Blast_Score=114, Evalue=3e-26,
Organism=Escherichia coli, GI1789183, Length=487, Percent_Identity=26.2833675564682, Blast_Score=82, Evalue=2e-16,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 95411; Mature: 95411

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLYVASAGTGKTHTLVEELLALVKGGVPLRRIAAVTFTRKAAEELRARARKGVEALARE
CEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EPGLGGAVREVHGAVFGTIHGFMAEALRHAAPFLSLDPDFAVVDELLAEALFLEEARSLL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
YLLGEDPGLEAFLRRLYQGRTLAEAFRPLEGAEGLVRLFAEALARYRRRAQEVLGPGDLE
HHHCCCCCHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
ALALRLLRHPQALARVVERFPHVLVDEYQDTSPLQGRFFRALEEAGARVVAVGDPKQSIY
HHHHHHHHCHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEECCCCHHEE
LFRNARLEVFREALGRAEEVLVLDETRRHARRVAAFLNRFVALFPEGERVAVRPTREAEG
EHHHHHHHHHHHHHCCHHEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCC
RVEVHWVEGGAGLEERRRFEADLLARRLLALKAEGYAFGEMAVLVRSRASLPLLERAFRA
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHH
RGVPYVLRRGQSFFNRLEVRDLYHALRLALLEGPPGPEERRSLLAFLRSPFVGLNLGEVE
CCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHH
EALLREDPLPYLPQAVRDRVAWLRGLAGKRPLEALKALVRDEVFLSRLSPRARANLDALL
HHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCHHHHH
LLAGLERFPDLEALLEWLRLRALDPEASELPEGGEGVGLLTVHAAKGLEWPVVAVYDVAR
HHHHHHHCCCHHHHHHHHHHHCCCCCHHHCCCCCCCCEEEEEECCCCCCCCEEEEEEHHC
GEPGPASPVLVGPEGEVAVAGTRAHRDLAQRLAQAQREEAVRLLYVALSRARDVLLVTGS
CCCCCCCCEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
RSGRESPWAQALERLGLGPESQDPLVARHPVPQGWRPPRPAPAARREGPPAPYAEHLLPP
CCCCCCHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCC
RPLPLVHSPSAHLKAEPKPLAEALEGEAVSGFARALGTLVHYALARDLDPEDGRAMRALL
CCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
HQEVAFPFAEGEKERLLAEVRALLRRYRGMLGRELPALEERAEDYRELPLVLPLKGTVWH
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEECCCHHHH
GVLDRVYRVGDRWYLDDYKTDREVRPEAYRLQLALYRRALLEAWGVRAEARLVYLRAGRV
HHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHEEEEEECCCE
HAFGEAELEEALAALPS
EECCHHHHHHHHHCCCC
>Mature Secondary Structure
MKLYVASAGTGKTHTLVEELLALVKGGVPLRRIAAVTFTRKAAEELRARARKGVEALARE
CEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EPGLGGAVREVHGAVFGTIHGFMAEALRHAAPFLSLDPDFAVVDELLAEALFLEEARSLL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
YLLGEDPGLEAFLRRLYQGRTLAEAFRPLEGAEGLVRLFAEALARYRRRAQEVLGPGDLE
HHHCCCCCHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
ALALRLLRHPQALARVVERFPHVLVDEYQDTSPLQGRFFRALEEAGARVVAVGDPKQSIY
HHHHHHHHCHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEECCCCHHEE
LFRNARLEVFREALGRAEEVLVLDETRRHARRVAAFLNRFVALFPEGERVAVRPTREAEG
EHHHHHHHHHHHHHCCHHEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCC
RVEVHWVEGGAGLEERRRFEADLLARRLLALKAEGYAFGEMAVLVRSRASLPLLERAFRA
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHH
RGVPYVLRRGQSFFNRLEVRDLYHALRLALLEGPPGPEERRSLLAFLRSPFVGLNLGEVE
CCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHH
EALLREDPLPYLPQAVRDRVAWLRGLAGKRPLEALKALVRDEVFLSRLSPRARANLDALL
HHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCHHHHH
LLAGLERFPDLEALLEWLRLRALDPEASELPEGGEGVGLLTVHAAKGLEWPVVAVYDVAR
HHHHHHHCCCHHHHHHHHHHHCCCCCHHHCCCCCCCCEEEEEECCCCCCCCEEEEEEHHC
GEPGPASPVLVGPEGEVAVAGTRAHRDLAQRLAQAQREEAVRLLYVALSRARDVLLVTGS
CCCCCCCCEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
RSGRESPWAQALERLGLGPESQDPLVARHPVPQGWRPPRPAPAARREGPPAPYAEHLLPP
CCCCCCHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCC
RPLPLVHSPSAHLKAEPKPLAEALEGEAVSGFARALGTLVHYALARDLDPEDGRAMRALL
CCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
HQEVAFPFAEGEKERLLAEVRALLRRYRGMLGRELPALEERAEDYRELPLVLPLKGTVWH
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEECCCHHHH
GVLDRVYRVGDRWYLDDYKTDREVRPEAYRLQLALYRRALLEAWGVRAEARLVYLRAGRV
HHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHEEEEEECCCE
HAFGEAELEEALAALPS
EECCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]