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The map label for this gene is ppdK [H]

Identifier: 55980632

GI number: 55980632

Start: 628449

End: 631046

Strand: Direct

Name: ppdK [H]

Synonym: TTHA0663

Alternate gene names: 55980632

Gene position: 628449-631046 (Clockwise)

Preceding gene: 55980631

Following gene: 55980634

Centisome position: 33.97

GC content: 72.67

Gene sequence:

>2598_bases
ATGGCGAAGCGCGTCTACTTGCTCTCCGAGGCCCGGGGGCTTTCCCGGGACCTCTTGGGCGGCAAGGGCCACGGCCTTGC
GGAGATGGCGGCGGCGGGCCTGCCCGTCCCCCCGGCCTTCATCGTCACCACGGAGGCCTGCCGCCAGTACCTGAAGGCGG
GGGAGGTGCCGGGGCTATGGGGGGAGGTGCGGGCGGGGATGGCGGCCCTAGAGGGCCTAACGGGCAAGCGCTTTGGCCGG
GGGGAGGGGAAGGCCCCTCCCCTCCTCGTTTCCGTGCGGAGCGGGGCCCCGGTTTCCATGCCCGGGATGATGGACACCGT
CCTCAACCTCGGCCTCACCCTGGAGGGGGTGGAGGCCCTGGCCCGGGCCACGGGCAACCCCCGCTTCGCCTGGGACAGCC
TCCGCAGGCTCCTCGCCATGTACGGGGAGGTGGTCTTGGGGGAGGGGCCCGAGGTCTTTGAGGGGATGCTCTCCGCCCTC
AAGGCGGAGAGGGGGGTGGGGACGGACGCGGCCCTTGGGCCCGAGGACCTGGAGGAGCTCGCCTACCGCTACCTCCGCCA
CCTCGAGGCCCGCGGCACCCCCTTCCCCCTGGACCCCTGGGCCCAACTCCAGGGAGCCATTGAGGCCGTATTCCGGAGCT
GGCAGAACCCCAGGGCCAGGACCTACCGCCGCATCTACGGCATCCCCGAGGACCTGGGCACGGCGGTGGTGGTCCAGGCC
ATGGTCTTCGGCAACCTGGGGGAGGACTCGGGCACCGGGGTGGGCTTCACCCGCAACCCCGCCACCGGGGAGAAGGGCCT
CTACGGGGAGTACCTGAGGAACGCCCAGGGGGAGGACGTGGTGGCGGGCGTCCGCACCCCCGAGCCCCTGGAGCGCCTTA
AAGGGTACGCCCCCGGGCTCTACGAGGAGCTCCTCCAGGTGGCGGAGCGCCTGGAGCGGCACTTCCGCGACATGCAGGAC
TTTGAGTTCACCGTGGAGCGGGGAAGGCTTTTCCTCCTCCAGACCCGCGCGGGCAAGCGCACGGCCCAGGCGGCGGTGCG
GATCGCCGTGGAGATGGCCGAGGAGGGGCTCATCTCCCGGGAGGAGGCGGTCATGAGGGTGGAGGCCAACGCCCTCCCCG
GCCTCCTCCGGCCCAGCGTGGACCGGGACCAGGCCCCAAGGCCCCTCCTCAAGGGCCTCCCCGCAAGCCCCGGGGCCGCG
GTGGGCCACGCCGCCTTCACCAACGAGGCCGTGGAGCGCTTCCACGCCCAGGGGCTTCCCGCCGTCCTCGTCCGCCCCGA
GACCACCCCCGAGGACATCACCGGGATGTACCTCGCCAAGGGGATCCTCACCGCCCGGGGCGGCCTCACCTCCCACGCGG
CGGTGGTGGCCCGGGGCCTCGGGGTGCCCGCGGTGGTGGGGGCGGAGGCCCTTAGGGTCTTCCCCGGGGAGGGCCGGGCC
CTGGCGGAGGGGGTGGAGATCCGGGAGGGGGACCTCCTCACCCTGGACGGGGGCACGGGGGAGGTGTACCTGGGGGCGGT
GCCCCTGGTGGAGGCTGGGGGGGAGGCCCTTTTGGAGAAGCTCCTCTCCTGGGCGGAGCCCCATCGGCGCCTCGGGGTCA
GGGCCAACGCCGACACCCCCGAGGACGCCCAAAGGGCCAGGGCCTTCGGGGCCGAGGGGATCGGGCTATGCCGCACGGAG
CACATGTTCTTCCGGGAGGACCGCCTCCCTTGGGTGCGCCGCCTCATCCTGGCGGAGACCCCCGAGGAGGAGGCCTTGGC
CCTGGAGCGCCTCTTCCACTTCCAGAAGGAGGACTTCAAGGAGATCCTCCGCGCCATGGACGGCCTCCCCGTCACGGTGC
GCCTCCTGGACCCGCCGCTTCACGAGTTCCTTCCTCCCCTGGAGGCGCTCGCCCGCCGGGCCGAGGAGGGGGACGAGGAG
GCGGGGCGCCTCCTAAGGCGGGCGGAGGCCCTGAAGGAGGCGAACCCCATGCTCGGCTTCCGGGGGGCGAGGCTCCTCCT
CCTCAGGCCCGGCGTCTTCCGCATGCAGCTTAGGGCCCTTCTGGAGGCGGCCAGGGAGCTCAAGGAGGCGGGGCACGACC
CCAGGCCCGAGGTCATGGTCCCCCTGGTGGCCGACCCCAAGGAGGTGGAAAGGGCGCGGGCCCTCGCCGAGGAGCTCTTC
CGGGAGTACGGCCCCATCCCCTTCGGCACCATGGTGGAGACCCCCCGGGCCGCCCTCCTCGCCGCCGAGATCGCCCCTTT
GGTGGACTTCTTCAGCTTCGGCACCAACGACCTCACCCAGATGGCCTTCGGCCTCTCCCGGGACGACGCGGGGAAGTTCC
TGCCCCGGTACGTGGAGGAGGGGCTTTTCCCCTTTGACCCCACGGAGCGCCTGGACGAGAAGGGGGTGGGGCGGCTTCTC
AGGATGGCGGCGGAGGAGGGGAGGAAGGCGAACCCCAGGCTCAAGCTCGGCCTCTGCGGGGAGCACGGGGGGGAGGCGGG
AAGCGTCCGCTTCGTGGCGGACCTCTTGGACTACACCTCGGCGAGCCCCTTCCGGGTCCTCACCGCCCGCCTCGCCGCCG
CCCAGGCGGGGCTTTCCCGGGCCCTAAAGCCCGCCTAG

Upstream 100 bases:

>100_bases
CTCGCCGAGTGGCGGGCCCTCCTTTCCCCTTAGGCGAACGGTGTAGCTCTCCTCGGGCTACACGCTTCCACGCTTCCACG
GGCGGGGCCAGAGTGGAGGC

Downstream 100 bases:

>100_bases
AGCCGCACCACCACCTTCCCCGTGTGCCCCCGGTCCAGGAGGGCCCGGAAGGCGGCCTCCGCCTCGGCGAAGGGGAAGAC
GGGGCCCACCACGGGCCTAA

Product: pyruvate phosphate dikinase

Products: NA

Alternate protein names: Pyruvate, orthophosphate dikinase [H]

Number of amino acids: Translated: 865; Mature: 864

Protein sequence:

>865_residues
MAKRVYLLSEARGLSRDLLGGKGHGLAEMAAAGLPVPPAFIVTTEACRQYLKAGEVPGLWGEVRAGMAALEGLTGKRFGR
GEGKAPPLLVSVRSGAPVSMPGMMDTVLNLGLTLEGVEALARATGNPRFAWDSLRRLLAMYGEVVLGEGPEVFEGMLSAL
KAERGVGTDAALGPEDLEELAYRYLRHLEARGTPFPLDPWAQLQGAIEAVFRSWQNPRARTYRRIYGIPEDLGTAVVVQA
MVFGNLGEDSGTGVGFTRNPATGEKGLYGEYLRNAQGEDVVAGVRTPEPLERLKGYAPGLYEELLQVAERLERHFRDMQD
FEFTVERGRLFLLQTRAGKRTAQAAVRIAVEMAEEGLISREEAVMRVEANALPGLLRPSVDRDQAPRPLLKGLPASPGAA
VGHAAFTNEAVERFHAQGLPAVLVRPETTPEDITGMYLAKGILTARGGLTSHAAVVARGLGVPAVVGAEALRVFPGEGRA
LAEGVEIREGDLLTLDGGTGEVYLGAVPLVEAGGEALLEKLLSWAEPHRRLGVRANADTPEDAQRARAFGAEGIGLCRTE
HMFFREDRLPWVRRLILAETPEEEALALERLFHFQKEDFKEILRAMDGLPVTVRLLDPPLHEFLPPLEALARRAEEGDEE
AGRLLRRAEALKEANPMLGFRGARLLLLRPGVFRMQLRALLEAARELKEAGHDPRPEVMVPLVADPKEVERARALAEELF
REYGPIPFGTMVETPRAALLAAEIAPLVDFFSFGTNDLTQMAFGLSRDDAGKFLPRYVEEGLFPFDPTERLDEKGVGRLL
RMAAEEGRKANPRLKLGLCGEHGGEAGSVRFVADLLDYTSASPFRVLTARLAAAQAGLSRALKPA

Sequences:

>Translated_865_residues
MAKRVYLLSEARGLSRDLLGGKGHGLAEMAAAGLPVPPAFIVTTEACRQYLKAGEVPGLWGEVRAGMAALEGLTGKRFGR
GEGKAPPLLVSVRSGAPVSMPGMMDTVLNLGLTLEGVEALARATGNPRFAWDSLRRLLAMYGEVVLGEGPEVFEGMLSAL
KAERGVGTDAALGPEDLEELAYRYLRHLEARGTPFPLDPWAQLQGAIEAVFRSWQNPRARTYRRIYGIPEDLGTAVVVQA
MVFGNLGEDSGTGVGFTRNPATGEKGLYGEYLRNAQGEDVVAGVRTPEPLERLKGYAPGLYEELLQVAERLERHFRDMQD
FEFTVERGRLFLLQTRAGKRTAQAAVRIAVEMAEEGLISREEAVMRVEANALPGLLRPSVDRDQAPRPLLKGLPASPGAA
VGHAAFTNEAVERFHAQGLPAVLVRPETTPEDITGMYLAKGILTARGGLTSHAAVVARGLGVPAVVGAEALRVFPGEGRA
LAEGVEIREGDLLTLDGGTGEVYLGAVPLVEAGGEALLEKLLSWAEPHRRLGVRANADTPEDAQRARAFGAEGIGLCRTE
HMFFREDRLPWVRRLILAETPEEEALALERLFHFQKEDFKEILRAMDGLPVTVRLLDPPLHEFLPPLEALARRAEEGDEE
AGRLLRRAEALKEANPMLGFRGARLLLLRPGVFRMQLRALLEAARELKEAGHDPRPEVMVPLVADPKEVERARALAEELF
REYGPIPFGTMVETPRAALLAAEIAPLVDFFSFGTNDLTQMAFGLSRDDAGKFLPRYVEEGLFPFDPTERLDEKGVGRLL
RMAAEEGRKANPRLKLGLCGEHGGEAGSVRFVADLLDYTSASPFRVLTARLAAAQAGLSRALKPA
>Mature_864_residues
AKRVYLLSEARGLSRDLLGGKGHGLAEMAAAGLPVPPAFIVTTEACRQYLKAGEVPGLWGEVRAGMAALEGLTGKRFGRG
EGKAPPLLVSVRSGAPVSMPGMMDTVLNLGLTLEGVEALARATGNPRFAWDSLRRLLAMYGEVVLGEGPEVFEGMLSALK
AERGVGTDAALGPEDLEELAYRYLRHLEARGTPFPLDPWAQLQGAIEAVFRSWQNPRARTYRRIYGIPEDLGTAVVVQAM
VFGNLGEDSGTGVGFTRNPATGEKGLYGEYLRNAQGEDVVAGVRTPEPLERLKGYAPGLYEELLQVAERLERHFRDMQDF
EFTVERGRLFLLQTRAGKRTAQAAVRIAVEMAEEGLISREEAVMRVEANALPGLLRPSVDRDQAPRPLLKGLPASPGAAV
GHAAFTNEAVERFHAQGLPAVLVRPETTPEDITGMYLAKGILTARGGLTSHAAVVARGLGVPAVVGAEALRVFPGEGRAL
AEGVEIREGDLLTLDGGTGEVYLGAVPLVEAGGEALLEKLLSWAEPHRRLGVRANADTPEDAQRARAFGAEGIGLCRTEH
MFFREDRLPWVRRLILAETPEEEALALERLFHFQKEDFKEILRAMDGLPVTVRLLDPPLHEFLPPLEALARRAEEGDEEA
GRLLRRAEALKEANPMLGFRGARLLLLRPGVFRMQLRALLEAARELKEAGHDPRPEVMVPLVADPKEVERARALAEELFR
EYGPIPFGTMVETPRAALLAAEIAPLVDFFSFGTNDLTQMAFGLSRDDAGKFLPRYVEEGLFPFDPTERLDEKGVGRLLR
MAAEEGRKANPRLKLGLCGEHGGEAGSVRFVADLLDYTSASPFRVLTARLAAAQAGLSRALKPA

Specific function: Catalyzes the reversible phosphorylation of pyruvate and phosphate. In E.histolytica and C.symbiosus, PPDK functions in the direction of ATP synthesis [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=430, Percent_Identity=28.8372093023256, Blast_Score=120, Evalue=4e-28,
Organism=Escherichia coli, GI1787994, Length=701, Percent_Identity=26.8188302425107, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1788726, Length=423, Percent_Identity=26.9503546099291, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI48994992, Length=379, Percent_Identity=27.4406332453826, Blast_Score=100, Evalue=5e-22,
Organism=Escherichia coli, GI1789193, Length=410, Percent_Identity=25.8536585365854, Blast_Score=97, Evalue=5e-21,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR010121
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.1 [H]

Molecular weight: Translated: 93938; Mature: 93807

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKRVYLLSEARGLSRDLLGGKGHGLAEMAAAGLPVPPAFIVTTEACRQYLKAGEVPGLW
CCCEEEEHHHHCCCHHHHCCCCCCCHHHHHHCCCCCCCHHEEEHHHHHHHHHCCCCCCCH
GEVRAGMAALEGLTGKRFGRGEGKAPPLLVSVRSGAPVSMPGMMDTVLNLGLTLEGVEAL
HHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCHHHHHHH
ARATGNPRFAWDSLRRLLAMYGEVVLGEGPEVFEGMLSALKAERGVGTDAALGPEDLEEL
HHCCCCCCHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
AYRYLRHLEARGTPFPLDPWAQLQGAIEAVFRSWQNPRARTYRRIYGIPEDLGTAVVVQA
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHH
MVFGNLGEDSGTGVGFTRNPATGEKGLYGEYLRNAQGEDVVAGVRTPEPLERLKGYAPGL
HHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCEEECCCCCHHHHHHCCCCCHH
YEELLQVAERLERHFRDMQDFEFTVERGRLFLLQTRAGKRTAQAAVRIAVEMAEEGLISR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCH
EEAVMRVEANALPGLLRPSVDRDQAPRPLLKGLPASPGAAVGHAAFTNEAVERFHAQGLP
HHHHHHHCCCCCCCHHCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
AVLVRPETTPEDITGMYLAKGILTARGGLTSHAAVVARGLGVPAVVGAEALRVFPGEGRA
EEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHCCHHHEECCCCCCH
LAEGVEIREGDLLTLDGGTGEVYLGAVPLVEAGGEALLEKLLSWAEPHRRLGVRANADTP
HHCCCEECCCCEEEEECCCCEEEEECCHHHHCCCHHHHHHHHHHHCHHHHCCCCCCCCCC
EDAQRARAFGAEGIGLCRTEHMFFREDRLPWVRRLILAETPEEEALALERLFHFQKEDFK
HHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
EILRAMDGLPVTVRLLDPPLHEFLPPLEALARRAEEGDEEAGRLLRRAEALKEANPMLGF
HHHHHHCCCCEEEEECCCCHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
RGARLLLLRPGVFRMQLRALLEAARELKEAGHDPRPEVMVPLVADPKEVERARALAEELF
CCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHH
REYGPIPFGTMVETPRAALLAAEIAPLVDFFSFGTNDLTQMAFGLSRDDAGKFLPRYVEE
HHHCCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHC
GLFPFDPTERLDEKGVGRLLRMAAEEGRKANPRLKLGLCGEHGGEAGSVRFVADLLDYTS
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCC
ASPFRVLTARLAAAQAGLSRALKPA
CCHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AKRVYLLSEARGLSRDLLGGKGHGLAEMAAAGLPVPPAFIVTTEACRQYLKAGEVPGLW
CCEEEEHHHHCCCHHHHCCCCCCCHHHHHHCCCCCCCHHEEEHHHHHHHHHCCCCCCCH
GEVRAGMAALEGLTGKRFGRGEGKAPPLLVSVRSGAPVSMPGMMDTVLNLGLTLEGVEAL
HHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCHHHHHHH
ARATGNPRFAWDSLRRLLAMYGEVVLGEGPEVFEGMLSALKAERGVGTDAALGPEDLEEL
HHCCCCCCHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
AYRYLRHLEARGTPFPLDPWAQLQGAIEAVFRSWQNPRARTYRRIYGIPEDLGTAVVVQA
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHH
MVFGNLGEDSGTGVGFTRNPATGEKGLYGEYLRNAQGEDVVAGVRTPEPLERLKGYAPGL
HHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCEEECCCCCHHHHHHCCCCCHH
YEELLQVAERLERHFRDMQDFEFTVERGRLFLLQTRAGKRTAQAAVRIAVEMAEEGLISR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCH
EEAVMRVEANALPGLLRPSVDRDQAPRPLLKGLPASPGAAVGHAAFTNEAVERFHAQGLP
HHHHHHHCCCCCCCHHCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
AVLVRPETTPEDITGMYLAKGILTARGGLTSHAAVVARGLGVPAVVGAEALRVFPGEGRA
EEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHCCHHHEECCCCCCH
LAEGVEIREGDLLTLDGGTGEVYLGAVPLVEAGGEALLEKLLSWAEPHRRLGVRANADTP
HHCCCEECCCCEEEEECCCCEEEEECCHHHHCCCHHHHHHHHHHHCHHHHCCCCCCCCCC
EDAQRARAFGAEGIGLCRTEHMFFREDRLPWVRRLILAETPEEEALALERLFHFQKEDFK
HHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
EILRAMDGLPVTVRLLDPPLHEFLPPLEALARRAEEGDEEAGRLLRRAEALKEANPMLGF
HHHHHHCCCCEEEEECCCCHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
RGARLLLLRPGVFRMQLRALLEAARELKEAGHDPRPEVMVPLVADPKEVERARALAEELF
CCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHH
REYGPIPFGTMVETPRAALLAAEIAPLVDFFSFGTNDLTQMAFGLSRDDAGKFLPRYVEE
HHHCCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHC
GLFPFDPTERLDEKGVGRLLRMAAEEGRKANPRLKLGLCGEHGGEAGSVRFVADLLDYTS
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCC
ASPFRVLTARLAAAQAGLSRALKPA
CCHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2176881; 6257292; 11695893; 11468288; 8610096; 9753432 [H]